##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031619_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 137663 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.012813900612365 33.0 31.0 34.0 30.0 34.0 2 32.165701749925546 34.0 31.0 34.0 30.0 34.0 3 32.31454348663039 34.0 31.0 34.0 30.0 34.0 4 35.2996738411919 37.0 35.0 37.0 33.0 37.0 5 35.159425553707244 37.0 35.0 37.0 33.0 37.0 6 35.53338224504769 37.0 35.0 37.0 33.0 37.0 7 35.67400100244801 37.0 36.0 37.0 35.0 37.0 8 35.75674654772887 37.0 36.0 37.0 35.0 37.0 9 37.563143328272666 39.0 38.0 39.0 35.0 39.0 10-11 37.56899820576335 39.0 38.0 39.0 35.0 39.0 12-13 37.64088026557608 39.0 38.0 39.0 35.0 39.0 14-15 38.73584042189986 40.5 38.0 41.0 35.5 41.0 16-17 38.87319759121914 40.5 38.5 41.0 35.5 41.0 18-19 38.961623675206845 41.0 39.0 41.0 36.0 41.0 20-21 38.985624314449055 41.0 39.0 41.0 36.0 41.0 22-23 38.93087467220677 41.0 39.0 41.0 35.5 41.0 24-25 38.76355302441469 40.5 38.5 41.0 35.0 41.0 26-27 38.49269229931063 40.0 38.0 41.0 34.5 41.0 28-29 38.45669134044732 40.0 38.0 41.0 35.0 41.0 30-31 38.29358287993143 40.0 38.0 41.0 35.0 41.0 32-33 37.921899856896914 40.0 37.0 41.0 34.0 41.0 34-35 37.579262401662035 40.0 36.0 41.0 33.0 41.0 36-37 37.28008252035768 40.0 36.0 41.0 32.5 41.0 38-39 36.964609226880135 39.5 35.0 41.0 32.0 41.0 40-41 36.91726898295112 40.0 35.0 41.0 31.5 41.0 42-43 36.540744426607006 40.0 35.0 41.0 30.5 41.0 44-45 36.03901919905857 39.0 35.0 41.0 28.0 41.0 46-47 35.4749932806927 39.0 35.0 41.0 23.5 41.0 48-49 35.14114540580984 39.0 35.0 41.0 20.0 41.0 50-51 34.56088055614072 38.5 34.5 40.5 18.0 41.0 52-53 34.82505466247285 39.0 35.0 40.5 16.0 41.0 54-55 35.02990999760284 40.0 35.0 41.0 14.0 41.0 56-57 34.6694899864161 39.5 35.0 41.0 10.5 41.0 58-59 34.37904883665182 39.0 35.0 41.0 10.0 41.0 60-61 33.66325011077777 39.0 34.0 41.0 10.0 41.0 62-63 33.491359334025844 38.0 33.5 41.0 9.0 41.0 64-65 33.20891234391231 37.5 33.5 40.0 8.0 41.0 66-67 32.67384119189615 37.0 33.0 39.0 8.0 41.0 68-69 32.14658259663091 36.5 32.0 39.0 8.0 41.0 70-71 31.444331447084547 35.5 31.5 38.0 7.0 40.0 72-73 30.880919346520123 35.0 30.0 37.0 7.0 39.0 74-75 30.528991086929675 35.0 30.5 37.0 7.0 39.0 76-77 30.14423265510704 35.0 30.0 36.5 7.0 37.5 78-79 29.74865795457022 35.0 29.0 36.0 7.0 37.0 80-81 29.31461612779033 35.0 29.0 35.5 7.0 36.5 82-83 28.880443546922557 34.5 27.5 35.0 7.0 36.0 84-85 28.6658579284194 34.0 27.0 35.0 7.0 36.0 86-87 28.72722518033168 34.5 27.5 35.0 7.0 36.0 88-89 28.444905312248025 34.0 26.5 35.0 7.0 35.0 90-91 28.157373440939104 34.0 25.0 35.0 7.0 35.0 92-93 27.942580795130137 34.0 25.0 35.0 7.0 35.0 94-95 27.663918409449163 34.0 24.0 35.0 7.0 35.0 96-97 27.439428168789 34.0 23.5 35.0 7.0 35.0 98-99 27.60169399184966 34.0 24.0 35.0 7.0 35.0 100-101 27.03355658383153 33.5 21.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37250298552396544 1101 2 0.2112128886786735 1101 3 0.11085877000835609 1101 4 0.24619435081308438 1101 5 0.3683386154010222 1101 6 0.19464083278995048 1101 7 0.11477370852882984 1101 8 0.14950074704492522 1101 9 0.38321292544314645 1101 10-11 0.04773887924057618 1101 12-13 0.26759694789678434 1101 14-15 0.3875856242898905 1101 16-17 0.24395322687971088 1101 18-19 0.23888528376114238 1101 20-21 -0.14852511690776282 1101 22-23 0.16547480881338572 1101 24-25 0.6384457103757413 1101 26-27 0.4977583804342345 1101 28-29 0.4229676886483773 1101 30-31 0.18461074571599312 1101 32-33 0.011388973109298206 1101 34-35 0.402103989767177 1101 36-37 0.2698976300238698 1101 38-39 0.3471976811620294 1101 40-41 0.44664464545297733 1101 42-43 0.840678596901995 1101 44-45 1.4159326100963128 1101 46-47 1.1939194452352382 1101 48-49 1.0625678609629858 1101 50-51 1.2872735204695687 1101 52-53 1.679033441306892 1101 54-55 1.6393541535552743 1101 56-57 1.5683768089865637 1101 58-59 2.135768581742674 1101 60-61 1.7185887456476578 1101 62-63 1.8692316771815172 1101 64-65 1.9636442812422885 1101 66-67 1.739988671130284 1101 68-69 1.747871392939885 1101 70-71 1.5118883448276392 1101 72-73 1.6235161807652396 1101 74-75 1.3795658087390095 1101 76-77 1.432772392375636 1101 78-79 1.7521691600656766 1101 80-81 1.945181526054224 1101 82-83 1.7330376974798547 1101 84-85 1.6296119091299133 1101 86-87 1.2666965850618581 1101 88-89 1.4030723527918667 1101 90-91 1.6763943161205113 1101 92-93 1.727033294447235 1101 94-95 2.1126669693480125 1101 96-97 2.246688307446341 1101 98-99 2.0977035076715715 1101 100-101 1.752265043457676 1102 1 0.15134913937011873 1102 2 0.20337813084391598 1102 3 0.13863654778613466 1102 4 0.038929393548123414 1102 5 0.28251240457480975 1102 6 0.41437017751929517 1102 7 0.5093606031157236 1102 8 0.25313322567740215 1102 9 0.3743097915400142 1102 10-11 0.22081580231749598 1102 12-13 0.031485836785670074 1102 14-15 0.3945300687343334 1102 16-17 0.42682359725007757 1102 18-19 0.9000320074078587 1102 20-21 0.6087754528928144 1102 22-23 0.7377682561068326 1102 24-25 0.629542576472609 1102 26-27 0.8601159302917836 1102 28-29 0.8132810789617579 1102 30-31 0.6730366716419169 1102 32-33 0.7140243007446259 1102 34-35 0.44590740857059785 1102 36-37 0.2506668607930962 1102 38-39 0.31318770965206255 1102 40-41 0.7290520528603821 1102 42-43 0.22404754277093275 1102 44-45 -0.47616140699771137 1102 46-47 -1.8794423781265834 1102 48-49 -1.0887854153902978 1102 50-51 -0.6906467074506608 1102 52-53 -0.2436873564139006 1102 54-55 -0.9454036812025493 1102 56-57 -0.500889572779819 1102 58-59 -1.3195376860635903 1102 60-61 -1.4249297728708612 1102 62-63 -3.065953508003666 1102 64-65 -1.9870323569343427 1102 66-67 -1.5942349755933662 1102 68-69 -1.3854975529290599 1102 70-71 -0.7968723389330492 1102 72-73 -1.015728833479777 1102 74-75 -0.889664960491757 1102 76-77 -1.297818775715534 1102 78-79 -1.3468336889371706 1102 80-81 -0.45741818904548737 1102 82-83 -0.6403597384175796 1102 84-85 -0.9506943586763548 1102 86-87 -0.7512877454224736 1102 88-89 -0.7796199549004399 1102 90-91 -0.8057994160732171 1102 92-93 -1.0511006086866708 1102 94-95 -0.4941706374895958 1102 96-97 -0.9468658236077943 1102 98-99 -0.8732722758042115 1102 100-101 -0.8182477770551415 1103 1 -0.11650800348702361 1103 2 0.062174427140213595 1103 3 0.06621591286550199 1103 4 0.24825479037352238 1103 5 -0.0561119340495253 1103 6 -0.0065954309463123195 1103 7 0.10790557666069844 1103 8 -0.09706518702100908 1103 9 0.25889973862996385 1103 10-11 0.16278008803178068 1103 12-13 0.21848980503964555 1103 14-15 0.2581213385755987 1103 16-17 0.3332389411654262 1103 18-19 0.35770396507981417 1103 20-21 -0.2505168751495219 1103 22-23 0.19329074287932713 1103 24-25 0.05431109499112807 1103 26-27 -0.01323063055478002 1103 28-29 -0.252856487175805 1103 30-31 0.4747893171445625 1103 32-33 0.3664713906917143 1103 34-35 0.5044391546023448 1103 36-37 0.2104882893645268 1103 38-39 0.12089273610708773 1103 40-41 0.29485893116726913 1103 42-43 0.5398544210778127 1103 44-45 0.7892155771292764 1103 46-47 1.4229716430374424 1103 48-49 1.4046008279959494 1103 50-51 1.2584273666234154 1103 52-53 1.0245004742739283 1103 54-55 1.2883926150937413 1103 56-57 1.4705059298656877 1103 58-59 1.5746422081163018 1103 60-61 1.1516244599333731 1103 62-63 1.6504816771815172 1103 64-65 2.0364464790444927 1103 66-67 1.747200209591817 1103 68-69 2.1558384259069143 1103 70-71 1.8851713118606028 1103 72-73 2.0380079390069916 1103 74-75 1.3022993252225277 1103 76-77 1.0193108539140958 1103 78-79 1.114806522703038 1103 80-81 1.423203504076202 1103 82-83 1.7182712139633693 1103 84-85 1.389914106932114 1103 86-87 0.7587982334135042 1103 88-89 0.6623443308138448 1103 90-91 0.8535921183183142 1103 92-93 1.1727750526889906 1103 94-95 0.6075158704469104 1103 96-97 1.1546553404133704 1103 98-99 1.113500210968276 1103 100-101 1.4600260324686651 1104 1 -0.047368809347830165 1104 2 0.09155476902055426 1104 3 0.09803825718784509 1104 4 -0.025173170554438684 1104 5 0.11727023933264746 1104 6 0.03545279860191641 1104 7 -0.00488441112928939 1104 8 9.964735406455816E-4 1104 9 0.07658899381921458 1104 10-11 0.3244482809499729 1104 12-13 0.35734053764037554 1104 14-15 0.4906839148881801 1104 16-17 0.47525664568313175 1104 18-19 0.34518870256455614 1104 20-21 0.3124791565965097 1104 22-23 0.30436369770227856 1104 24-25 0.3916508385808726 1104 26-27 0.38931820949406415 1104 28-29 0.3460446117252971 1104 30-31 0.4169825405877887 1104 32-33 0.7603205970409235 1104 34-35 0.7749672376304275 1104 36-37 1.031649766776006 1104 38-39 0.6896122110765646 1104 40-41 0.4808326796410114 1104 42-43 0.812366631090029 1104 44-45 0.1926420118057095 1104 46-47 0.2580220093378074 1104 48-49 0.5251746985698134 1104 50-51 1.083213691409739 1104 52-53 1.4942043814778359 1104 54-55 2.0004652646663885 1104 56-57 1.3733981765079264 1104 58-59 0.9974138808879829 1104 60-61 0.9878195148784314 1104 62-63 0.9290607370105732 1104 64-65 1.089178469276483 1104 66-67 0.9595399531815616 1104 68-69 0.6565252390937317 1104 70-71 0.8270592849985761 1104 72-73 1.8179606252096825 1104 74-75 1.5911042702774694 1104 76-77 1.5155715376747807 1104 78-79 1.533152066048583 1104 80-81 1.34849349186619 1104 82-83 1.231435133377289 1104 84-85 1.1686076356256407 1104 86-87 1.523641029506301 1104 88-89 1.5686706433901563 1104 90-91 1.6454114101376085 1104 92-93 1.7451956876096268 1104 94-95 1.5742054308864724 1104 96-97 1.2632481365061707 1104 98-99 1.2050753025433671 1104 100-101 1.2586752998679316 1105 1 -0.4263819530668549 1105 2 -0.23351885017071083 1105 3 -0.10342694427735921 1105 4 0.10382201726427809 1105 5 -0.15695227018397873 1105 6 -0.7571206410303475 1105 7 0.10055263548422744 1105 8 0.18077094743276945 1105 9 0.10711402434424855 1105 10-11 -0.16678923969931247 1105 12-13 -0.03492406050657593 1105 14-15 -0.16966227486977203 1105 16-17 -0.06539551261609233 1105 18-19 -0.16540527861766208 1105 20-21 -0.3873341020402705 1105 22-23 -0.297518080650093 1105 24-25 -0.21328344282400025 1105 26-27 -0.5137558406388152 1105 28-29 -0.40411699137748514 1105 30-31 -0.15520017865375024 1105 32-33 0.21363525623793578 1105 34-35 -0.26604403867496984 1105 36-37 -0.31130792912286864 1105 38-39 -0.16350852439711616 1105 40-41 -0.14159064866466764 1105 42-43 0.3040350933467266 1105 44-45 0.13611683763347315 1105 46-47 0.21945273547441957 1105 48-49 0.6630041893404837 1105 50-51 0.42229291284190396 1105 52-53 0.13742064234116214 1105 54-55 0.6282035394634846 1105 56-57 0.9124702155799653 1105 58-59 0.48850775433479754 1105 60-61 0.6009416868241289 1105 62-63 0.6171308368453836 1105 64-65 0.7796187479520498 1105 66-67 0.22855525160861845 1105 68-69 0.6878762410329671 1105 70-71 0.5219885387513585 1105 72-73 0.6905814684187597 1105 74-75 0.8813434428695857 1105 76-77 0.26878564383006065 1105 78-79 -0.056411964691918115 1105 80-81 0.6464177897904868 1105 82-83 0.9086598694255557 1105 84-85 0.9424351153354742 1105 86-87 0.5426742838336729 1105 88-89 0.6090355072844318 1105 90-91 0.49644926117546007 1105 92-93 0.782543960252017 1105 94-95 0.4591440217074165 1105 96-97 0.971357021085641 1105 98-99 0.7353489504640756 1105 100-101 0.5795113265863137 1106 1 0.1777719035977583 1106 2 -0.2503255728597864 1106 3 -0.1289786283656298 1106 4 0.46304863474053803 1106 5 0.3050959637437387 1106 6 -0.029170924558393097 1106 7 0.06536782985465805 1106 8 0.051236206707208964 1106 9 -0.07871640538861868 1106 10-11 -0.024102896869500512 1106 12-13 0.011499386921173027 1106 14-15 0.04967912022484455 1106 16-17 0.12962395858702536 1106 18-19 0.15590373279177783 1106 20-21 0.008956121366161085 1106 22-23 0.28508081256573803 1106 24-25 0.005784672226901932 1106 26-27 0.19503011276693627 1106 28-29 -0.02231061028846426 1106 30-31 0.12617578636639593 1106 32-33 0.2630306241411944 1106 34-35 0.02585320802858604 1106 36-37 0.024040844532933647 1106 38-39 0.3679528406367041 1106 40-41 0.16615684057492786 1106 42-43 -0.5166931979698006 1106 44-45 -0.12995544493809064 1106 46-47 0.14607704373430863 1106 48-49 -0.9894177550470289 1106 50-51 -1.0342555602058567 1106 52-53 -0.7771110239839132 1106 54-55 -0.6572374662070715 1106 56-57 -0.8993764743154955 1106 58-59 -1.097541299433054 1106 60-61 -1.1350697909377132 1106 62-63 -0.7080040951762072 1106 64-65 -0.15889324220986367 1106 66-67 -0.9710416603268825 1106 68-69 -0.9923177877980791 1106 70-71 -1.7779021492311529 1106 72-73 -1.3891479843379528 1106 74-75 -1.3591771556137076 1106 76-77 -1.4893273574680173 1106 78-79 -0.6048508524421408 1106 80-81 -0.5835473670039342 1106 82-83 -0.37613209614115917 1106 84-85 -0.32117764684256755 1106 86-87 -0.4536364355760405 1106 88-89 -0.47677442644515367 1106 90-91 -0.30320230676879234 1106 92-93 -0.0447772121193708 1106 94-95 -0.06056630144623654 1106 96-97 -0.5432976212591001 1106 98-99 -0.296488174629868 1106 100-101 0.11264653188794682 1107 1 0.30904144706242676 1107 2 0.20352058098636405 1107 3 -0.16606430691471985 1107 4 0.4577328123515443 1107 5 0.416415538477942 1107 6 0.023486986636100937 1107 7 0.022466016221137863 1107 8 0.22834690089107568 1107 9 0.39282831005853325 1107 10-11 0.4688927253944186 1107 12-13 0.5368277171275508 1107 14-15 0.4077779319822028 1107 16-17 0.39491476534124814 1107 18-19 0.6494622068380593 1107 20-21 0.5043594984768589 1107 22-23 0.36547480881338856 1107 24-25 0.48652263345266533 1107 26-27 0.1602583804342359 1107 28-29 0.35373691941760654 1107 30-31 0.08749536110060774 1107 32-33 0.28254281926314206 1107 34-35 0.6001809128441025 1107 36-37 0.9025899377161721 1107 38-39 0.4096976811620294 1107 40-41 0.5658754146837452 1107 42-43 0.2858709045942973 1107 44-45 0.32554799471169105 1107 46-47 0.2169963683121665 1107 48-49 0.28756786096298015 1107 50-51 -0.018495710299660573 1107 52-53 0.6078795951530509 1107 54-55 1.0624310766322083 1107 56-57 1.017415270525028 1107 58-59 1.4482685817426741 1107 60-61 1.3762810533399659 1107 62-63 1.3615393694892077 1107 64-65 2.0588365889346036 1107 66-67 1.3928732865148916 1107 68-69 1.0757560083244968 1107 70-71 1.244580652519943 1107 72-73 1.3965931038421608 1107 74-75 2.029565808739008 1107 76-77 1.620272392375636 1107 78-79 1.6079383908349065 1107 80-81 1.5749892183619174 1107 82-83 1.533999235941394 1107 84-85 1.2574965245145293 1107 86-87 1.244581200446472 1107 88-89 1.0780723527918674 1107 90-91 1.436009700735898 1107 92-93 0.8174179098318497 1107 94-95 1.0011285078095469 1107 96-97 1.0149575382155689 1107 98-99 1.1602035076715715 1107 100-101 1.502265043457676 1108 1 -0.8054177779231146 1108 2 -0.2954383548146744 1108 3 -0.3665848390142017 1108 4 -0.545448217145271 1108 5 -0.3794202047262232 1108 6 -0.4271781377132342 1108 7 -0.5810041977753926 1108 8 -0.41144488626913045 1108 9 -0.013186727535455134 1108 10-11 -0.11283081422385521 1108 12-13 -0.1948090671408096 1108 14-15 -0.4841249020258971 1108 16-17 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-1.0189526069915082 2316 74-75 -1.1684607720100821 2316 76-77 -0.8697598267141764 2316 78-79 -0.5891014802887327 2316 80-81 -0.684173085303005 2316 82-83 -0.7511799827456791 2316 84-85 -1.0216416148330332 2316 86-87 -0.8726761495333868 2316 88-89 -0.8516094836962509 2316 90-91 -1.186422840544811 2316 92-93 -1.071849105874957 2316 94-95 -0.43948768228307955 2316 96-97 -0.1016554299680763 2316 98-99 -0.33003008072955353 2316 100-101 -0.38718320462931644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 18.0 8 106.0 9 142.0 10 111.0 11 150.0 12 207.0 13 227.0 14 304.0 15 421.0 16 758.0 17 1272.0 18 1530.0 19 2102.0 20 2822.0 21 3132.0 22 2256.0 23 2022.0 24 1948.0 25 1716.0 26 1527.0 27 1672.0 28 1833.0 29 1935.0 30 2140.0 31 2609.0 32 3263.0 33 4268.0 34 6059.0 35 9982.0 36 16934.0 37 27334.0 38 31824.0 39 5022.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.82244150232823 20.19428671522618 12.954150670734377 15.029121111711213 2 13.960236510378316 27.995567253082143 32.93866332266461 25.105532913874935 3 29.252032401982547 26.378649044025064 31.321916462274107 13.04740209171828 4 10.429876606122376 16.242856622866086 44.85640446984312 28.47086230116842 5 8.082485036480405 45.6842442512415 34.1838146416765 12.0494560706016 6 23.510789272588333 39.83916160256177 16.321822349987265 20.32822677486263 7 22.518484019328174 37.790359201257495 22.06147755719858 17.629679222215756 8 24.086109150188857 24.083198090344744 20.98291935636467 30.847773403101737 9 21.256885874587937 22.864378288300742 26.815796942198677 29.062938894912637 10-11 24.964702114961938 23.804602553092387 27.416267594358164 23.81442773758752 12-13 32.660546452859435 24.854908724397532 24.841809574750663 17.642735247992373 14-15 23.94895140776646 27.66514918285134 33.16338970221654 15.222509707165655 16-17 17.377032494465507 30.1385349681744 32.050979123423716 20.433453413936377 18-19 15.507706618026438 35.89154779120552 31.08735620489939 17.513389385868656 20-21 20.793168825319803 26.981469240100825 28.46407531435462 23.76128662022475 22-23 16.63845768289228 29.338674879960486 30.0614544213042 23.961413015843036 24-25 23.630169326543808 28.840719728612623 27.575310722561618 19.953800222281952 26-27 21.489434343287595 23.141657525987373 37.55366365690128 17.815244473823757 28-29 16.685674436849407 35.957010961551035 30.950945424696542 16.406369176903016 30-31 18.917210870023172 27.972294661601154 29.567494533752715 23.54299993462296 32-33 18.123242991944092 30.457348742944728 32.65547024254884 18.763938022562343 34-35 22.83983350646143 28.074718697108153 30.102496676666934 18.98295111976348 36-37 21.077558966461577 26.02805401596653 34.78131378801857 18.11307322955333 38-39 18.557273922549996 31.740191627379904 32.64747971495609 17.055054735114012 40-41 17.07575746569521 33.33829714592883 30.67890428074356 18.90704110763241 42-43 16.054059551222913 33.606706231885106 26.047303923349048 24.291930293542926 44-45 20.4068568435678 27.004731993147523 28.09516361930188 24.493247543982804 46-47 23.26556460594414 31.302091294884953 29.180951965001928 16.251392134168977 48-49 20.924382272857812 30.193652051577537 28.939476008884657 19.942489666679997 50-51 13.051648311707192 31.07476465670774 35.629338857994405 20.244248173590666 52-53 18.752726162435664 34.49358806595132 25.726613161185263 21.02707261042775 54-55 16.286594032986887 28.22280144976534 28.13991616953675 27.35068834771102 56-57 21.593148841776063 25.523109931270227 28.2472089894178 24.63653223753591 58-59 26.64179837047217 25.074804852953473 26.798134150642248 21.485262625932105 60-61 20.141778450257515 28.7987022434312 32.93130037536008 18.128218930951203 62-63 16.293286437796056 28.24036147429666 32.59021383345531 22.87613825445198 64-65 15.383524328727866 34.69812302018817 28.384546284409172 21.53380636667479 66-67 20.472569789132642 26.29314890166432 25.390262793310715 27.844018515892323 68-69 17.294272092862574 26.72780895096859 29.086829559215527 26.891089396953312 70-71 23.337768858252257 26.474630251734215 27.718031499498274 22.469569390515247 72-73 21.554365724131163 20.947333602317627 35.4052393778466 22.09306129570461 74-75 16.5797925934548 32.41172854549886 29.307177750537434 21.701301110508915 76-77 19.20728710449 25.345041630715347 28.50475285517171 26.94291840962294 78-79 20.009821396093265 24.994725546542504 31.058164490196795 23.937288567167435 80-81 23.89673272941416 23.572841085891266 29.24112660491763 23.289299579776948 82-83 20.630644387243137 23.061457469860244 33.294795166004974 23.01310297689164 84-85 18.76499847288276 26.530026033712932 31.234637927774628 23.47033756562968 86-87 19.68957871396896 28.133110392206756 28.484969648504233 23.692341245320055 88-89 18.619516132550885 28.317226233847453 25.069627755113917 27.993629878487752 90-91 21.46182156728685 22.935426152480982 26.710719636908486 28.892032643323684 92-93 24.790883655270022 27.01575785800827 27.014302502847038 21.179055983874665 94-95 22.432411788882543 24.986815161291496 28.294845728294117 24.28592732153184 96-97 17.64042983234212 25.48930508499222 32.82997193584505 24.040293146820606 98-99 21.99794992584838 28.906045537817327 24.149800808398034 24.946203727936258 100-101 19.718898565052413 24.005568236337467 27.3661369815216 28.909396217088524 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 348.0 1 218.0 2 121.5 3 174.5 4 137.0 5 96.0 6 174.0 7 332.0 8 434.5 9 404.0 10 279.5 11 179.5 12 142.5 13 113.5 14 134.0 15 144.0 16 136.5 17 133.5 18 133.5 19 153.5 20 175.5 21 187.5 22 213.0 23 279.5 24 348.0 25 440.5 26 582.0 27 864.5 28 1212.0 29 1421.0 30 1671.0 31 2231.5 32 2955.5 33 3574.0 34 4524.0 35 5049.0 36 4542.0 37 4919.0 38 4637.0 39 3542.0 40 3343.5 41 3057.5 42 2475.5 43 2102.5 44 1795.5 45 1572.0 46 2721.5 47 8967.5 48 20911.0 49 20222.0 50 9674.0 51 4561.0 52 1980.5 53 1167.0 54 792.5 55 573.0 56 474.5 57 345.0 58 240.5 59 195.0 60 178.5 61 186.0 62 174.0 63 167.5 64 176.0 65 181.5 66 171.5 67 176.0 68 186.5 69 180.5 70 195.0 71 185.0 72 151.5 73 156.0 74 159.5 75 141.0 76 119.0 77 102.0 78 86.0 79 68.0 80 42.0 81 32.0 82 27.5 83 14.0 84 12.5 85 10.5 86 7.5 87 6.0 88 4.0 89 2.0 90 2.0 91 2.0 92 1.0 93 1.5 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47144112797193144 2 0.36465862286888995 3 0.19177266222587042 4 0.21647065660344464 5 0.2389894161829976 6 0.18741419262982792 7 0.17942366503708332 8 0.18596136943114708 9 0.17724443023906206 10-11 0.18959342742784915 12-13 0.18123969403543436 14-15 0.1921358680255406 16-17 0.08462695132315873 18-19 0.03922622636438258 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.14128705607171135 46-47 0.20630089421267878 48-49 0.08898542091920124 50-51 0.071188336735361 52-53 0.07772604112942476 54-55 0.08971183251854166 56-57 0.12203714868919027 58-59 0.10060800650864793 60-61 0.14165026187138158 62-63 0.24443750317805074 64-65 0.1318437052802859 66-67 0.11586265009479672 68-69 0.12312676608820089 70-71 0.09951838910963731 72-73 0.07881565852843538 74-75 0.1478247604657751 76-77 0.23572056398596572 78-79 0.15109361266280702 80-81 0.08499015712282894 82-83 0.09951838910963731 84-85 0.10823532830172232 86-87 0.07845245272876517 88-89 0.10605609350370106 90-91 0.12857485308325403 92-93 0.17397557804203018 94-95 0.14128705607171135 96-97 0.08789580352019061 98-99 0.07772604112942476 100-101 0.07046192513602058 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 137663.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.920733966279972 #Duplication Level Percentage of deduplicated Percentage of total 1 81.0780443364713 19.394463290789826 2 7.740662010324932 3.7032463334374524 3 2.991193440631643 2.146546276050936 4 1.6337686000607348 1.5632377617805802 5 1.0173094442757364 1.2167394288952007 6 0.6620103249316732 0.950146371937267 7 0.583054965077437 0.9762971895135222 8 0.41299726692985117 0.7903358200823751 9 0.3583358639538415 0.7714491184995241 >10 2.711812936532038 13.018748683378975 >50 0.41299726692985117 6.938683596899676 >100 0.31278469480716675 13.039088208160507 >500 0.03036744609778318 5.107399954962481 >1k 0.051624658366231396 23.77545164641189 >5k 0.0030367446097783178 6.608166319199785 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9097 6.608166319199785 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4807 3.4918605580293907 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3388 2.4610824985653372 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2929 2.127659574468085 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2129 1.5465302949957505 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2035 1.4782476046577513 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2030 1.474615546661049 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1874 1.3612953371639438 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1847 1.3416822239817525 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1701 1.2356261304780516 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1656 1.2029376085077326 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1343 0.9755707779141818 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1251 0.9087409107748632 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1209 0.8782316236025658 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1198 0.8702410960098211 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1197 0.8695146844104806 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1071 0.777986822893588 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1065 0.7736283532975454 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 902 0.6552232626050573 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 834 0.6058272738499089 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 758 0.550619992300037 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 721 0.5237427631244416 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 687 0.4990447687468673 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 664 0.4823373019620377 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 661 0.4801580671640165 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 653 0.4743467743692931 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 598 0.4343941364055701 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 553 0.4017056144352513 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 482 0.35013039088208164 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 411 0.2985551673289119 No Hit TATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAA 349 0.25351764816980593 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 346 0.2513384133717847 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 342 0.248432766974423 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 334 0.2426214741796997 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 310 0.22518759579552966 No Hit ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA 298 0.21647065660344464 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 298 0.21647065660344464 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 294 0.21356501020608296 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 286 0.20775371741135965 No Hit GTCCTAGCCTTAATCTTATCTATCCTAATTTTAGCCCTAATACCTTTCCT 270 0.19613113182191294 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 264 0.19177266222587042 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 259 0.18814060422916834 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 245 0.17797084183840248 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 241 0.17506519544104082 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 240 0.1743387838417004 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 237 0.17215954904367914 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 232 0.16852749104697703 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 224 0.1627161982522537 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 222 0.16126337505357285 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 218 0.15835772865621117 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 215 0.15617849385818994 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 212 0.15399925906016868 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 208 0.15109361266280702 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 207 0.15036720106346657 No Hit GTACATGGGAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGAC 196 0.142376673470722 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 191 0.1387446154740199 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 190 0.13801820387467947 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 188 0.13656538067599863 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 188 0.13656538067599863 No Hit ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA 188 0.13656538067599863 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 185 0.13438614587797737 No Hit ATATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCA 183 0.13293332267929656 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 181 0.13148049948061571 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 180 0.1307540878812753 No Hit TTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTT 173 0.12566920668589235 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 173 0.12566920668589235 No Hit CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC 172 0.12494279508655194 No Hit ATTCTACGCTCAATCCCCAATAAACTAGGAGGTGTCCTAGCCTTAATCTT 170 0.12348997188787111 No Hit GAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATA 170 0.12348997188787111 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 167 0.12131073708984985 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 165 0.11985791389116901 No Hit GGTTAAGATAAGTAGGTTGGCTACTAGGATTCAGTACAAAATTTGTGTGA 165 0.11985791389116901 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 157 0.11404662109644567 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 155 0.11259379789776483 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 154 0.11186738629842441 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 154 0.11186738629842441 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 152 0.11041456309974357 No Hit GTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTAT 151 0.10968815150040317 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 151 0.10968815150040317 No Hit CTCATAACCCTAGTATTATTTTTCCCAGACATACTAGGAGACCCAGACAA 150 0.10896173990106274 No Hit CGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACAT 150 0.10896173990106274 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 148 0.1075089167023819 No Hit CATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 148 0.1075089167023819 No Hit ATCCTAGGTATCCTAATCATATTCTTAATTCTCATAACCCTAGTATTATT 147 0.10678250510304148 No Hit GTATTAGGGCTAAAATTAGGATAGATAAGATTAAGGCTAGGACACCTCCT 146 0.10605609350370106 No Hit GTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCA 145 0.10532968190436064 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 145 0.10532968190436064 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 144 0.10460327030502024 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 139 0.10097121230831813 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.264115993404183E-4 0.0 14-15 0.0 0.0 0.0 0.003268852197031882 0.0 16-17 0.0 0.0 0.0 0.02360837697856359 0.0 18-19 0.0 0.0 0.0 0.0359573741673507 0.0 20-21 0.0 0.0 0.0 0.05847613374690368 0.0 22-23 0.0 0.0 0.0 0.13765499807500928 0.0 24-25 0.0 0.0 0.0 0.24879597277409324 0.0 26-27 0.0 0.0 0.0 0.2905646397361673 0.0 28-29 0.0 0.0 0.0 0.36138977067185807 0.0 30-31 0.0 0.0 0.0 0.45945533658281457 0.0 32-33 0.0 0.0 0.0 0.6348837378235256 0.0 34-35 0.0 0.0 0.0 0.8818636815992678 0.0 36-37 0.0 0.0 0.0 1.1335653007707227 0.0 38-39 0.0 0.0 0.0 1.4942286598432404 0.0 40-41 0.0 0.0 0.0 1.9315284426461723 0.0 42-43 0.0 0.0 0.0 2.268220218940456 0.0 44-45 0.0 0.0 0.0 2.5972846734416657 0.0 46-47 0.0 0.0 0.0 3.0298627808488847 0.0 48-49 0.0 0.0 0.0 3.367280968742509 0.0 50-51 0.0 0.0 0.0 3.769712994777101 0.0 52-53 0.0 0.0 0.0 4.111852858066438 0.0 54-55 0.0 0.0 0.0 4.459804014150498 0.0 56-57 0.0 0.0 0.0 4.975556249682195 0.0 58-59 0.0 0.0 0.0 5.395058948301287 0.0 60-61 0.0 0.0 0.0 5.816377675918729 0.0 62-63 0.0 0.0 0.0 6.1988333829714595 0.0 64-65 0.0 0.0 0.0 6.548963773853541 0.0 66-67 0.0 0.0 0.0 7.021131313424813 0.0 68-69 0.0 0.0 0.0 7.633496291668785 0.0 70-71 0.0 0.0 0.0 8.297073287666258 0.0 72-73 0.0 0.0 0.0 8.743090009661275 0.0 74-75 0.0 0.0 0.0 9.238139514611769 0.0 76-77 0.0 0.0 0.0 9.698684468593594 0.0 78-79 0.0 0.0 0.0 10.569651976202756 0.0 80-81 0.0 0.0 0.0 11.42790728082346 0.0 82-83 0.0 0.0 0.0 12.01811670528755 0.0 84-85 0.0 0.0 0.0 12.653726854710417 0.0 86-87 0.0 0.0 0.0 13.27008709675076 0.0 88-89 0.0 0.0 0.0 14.038630568852923 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGGGA 35 3.5448465E-8 81.50351 4 GGATATA 20 0.0020477653 71.44572 1 GATATAA 20 0.0020477653 71.44572 2 GCTGGAC 20 0.0020626355 71.315575 3 TATATGG 55 1.1477823E-8 60.6206 2 GGGCTTC 40 7.485576E-6 59.408005 7 GTATATG 65 6.475602E-10 58.622124 1 AGTACTC 50 4.187641E-7 57.05246 5 TAACTTT 120 0.0 55.447468 6 AACTTTA 125 0.0 53.229572 7 ATGGGAC 45 1.5007272E-5 52.82635 5 GGACTTC 45 1.5039692E-5 52.807114 8 GACTTCT 45 1.5039692E-5 52.807114 9 GGCTTCT 45 1.5039692E-5 52.807114 8 GGGACTT 45 1.5039692E-5 52.807114 7 ATAACTT 145 0.0 49.18316 5 TTATAGG 70 7.6848664E-8 47.630478 2 CTTATGT 60 1.4548441E-6 47.630478 2 GCTTATG 60 1.4548441E-6 47.630478 1 TATGTAC 60 1.4732541E-6 47.54372 4 >>END_MODULE