FastQCFastQC Report
Fri 10 Feb 2017
SRR2031619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137663
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT99357.216899239447056No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT46273.3611064701481155No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32012.325243529488679No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27371.9881885473947247No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22051.6017375765456223No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21791.5828508749627717No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT21351.550888764591793No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC20121.4615401378729214No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA19011.380908450346135No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG17481.2697674756470512No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC15911.1557208545506055No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC14001.0169762390765855No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12790.929080435556395No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA12770.9276276123577142No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC12490.9072880875761824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12350.8971183251854165No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG10670.7750811764962262No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA10250.7445718893239287No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG8660.6290724450288022No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT8590.6239875638334192No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC7940.5767708098762921No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.5419030531079521No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6930.5034032383429098No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT6910.501950415144229No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC6530.4743467743692931No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6080.44165825239897427No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5910.4293092552101872No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG5790.42059231601810215No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5360.3893566172464642No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT5160.37482838525965584No Hit
TATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAA4260.3094513413190182No Hit
ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA3930.2854797585407844No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA3660.2658666453585931No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.2636874105605718No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG3550.2578761177658485No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG3450.2506120017724443No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3360.24407429737838054No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT3290.2389894161829976No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC3270.23753659298431676No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA3060.22228194939816798No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT3010.2186498914014659No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC2950.2142914218054234No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG2700.19613113182191294No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT2640.19177266222587042No Hit
GTCCTAGCCTTAATCTTATCTATCCTAATTTTAGCCCTAATACCTTTCCT2490.18087648823576413No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.17724443023906206No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC2430.17651801863972164No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.17361237224235998No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT2350.1707067258449983TruSeq Adapter, Index 11 (95% over 21bp)
ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA2310.16780107944763661No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2230.16198978665291328No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA2220.16126337505357285No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA2160.15690490545753033No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA2060.14964078946412615No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA2000.14528231986808365No Hit
GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC1970.1431030850700624No Hit
ATTCTACGCTCAATCCCCAATAAACTAGGAGGTGTCCTAGCCTTAATCTT1970.1431030850700624No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1890.13729179227533905No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT1850.13438614587797737No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA1830.13293332267929656No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA1820.13220691107995614No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG1800.1307540878812753No Hit
GTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCA1800.1307540878812753No Hit
GTATTAGGGCTAAAATTAGGATAGATAAGATTAAGGCTAGGACACCTCCT1790.13002767628193487No Hit
ATCCTAGGTATCCTAATCATATTCTTAATTCTCATAACCCTAGTATTATT1740.12639561828523277No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1740.12639561828523277No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1730.12566920668589235No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA1690.1227635602885307No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA1670.12131073708984985No Hit
GAATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTA1660.12058432549050943No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1640.1191315022918286No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG1630.11840509069248818No Hit
CTCATAACCCTAGTATTATTTTTCCCAGACATACTAGGAGACCCAGACAA1610.11695226749380734No Hit
ATATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCA1610.11695226749380734No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC1600.11622585589446692No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC1590.1154994442951265No Hit
GGTTAAGATAAGTAGGTTGGCTACTAGGATTCAGTACAAAATTTGTGTGA1560.11332020949710525No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC1560.11332020949710525No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.11186738629842441No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT1530.11114097469908399No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1520.11041456309974357No Hit
AATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACCTTTTGCATAAT1520.11041456309974357No Hit
CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC1510.10968815150040317No Hit
TTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTT1500.10896173990106274No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1410.10242403550699897No Hit
GAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATA1390.10097121230831813No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1390.10097121230831813No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC1390.10097121230831813No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT1390.10097121230831813No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATATA200.002061843971.322551
TATATAA200.002070784871.244835
GATATAA200.002070784871.244832
ATGGGAT250.005013356556.995865
GCAAAGC250.005013356556.995863
ATAAGCA250.005013356556.995865
CGCAGAA1100.056.1322869
AACTTTA1100.051.814427
ACTTTAT1100.051.814428
TAACTTT1150.049.561626
ATGGGAG405.3734897E-447.496555
AAGTACT405.3734897E-447.496554
AAAGTAC405.3734897E-447.496553
TGGGCAG405.3734897E-447.496556
ATAACTT1200.047.4965485
TATAACT1250.045.5966874
CTATAAC1300.043.842973
ATGGGCA554.942595E-543.178685
AGTACTC459.598959E-442.219165
ATCTATA1400.040.755741
Produced by FastQC (version 0.11.5)