##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031619_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 137663 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16978418311383 33.0 31.0 34.0 30.0 34.0 2 32.33077152175966 34.0 31.0 34.0 30.0 34.0 3 32.42234296797251 34.0 31.0 34.0 30.0 34.0 4 35.8439449961137 37.0 35.0 37.0 35.0 37.0 5 35.62257106121471 37.0 35.0 37.0 33.0 37.0 6 35.879219543377666 37.0 35.0 37.0 35.0 37.0 7 35.8221308557855 37.0 35.0 37.0 35.0 37.0 8 35.950480521272965 37.0 35.0 37.0 35.0 37.0 9 37.69485627946507 39.0 37.0 39.0 35.0 39.0 10-11 37.65718820598127 39.0 37.0 39.0 35.0 39.0 12-13 37.7311623312001 39.0 38.0 39.0 35.0 39.0 14-15 38.708236781124924 40.0 38.0 41.0 34.0 41.0 16-17 38.841664063691766 40.0 38.0 41.0 35.0 41.0 18-19 39.051756826453 40.5 38.5 41.0 35.5 41.0 20-21 39.09330756993528 40.5 39.0 41.0 35.5 41.0 22-23 38.99170074747754 40.0 38.5 41.0 35.0 41.0 24-25 38.792787459230155 40.0 38.0 41.0 34.5 41.0 26-27 38.44668502066641 40.0 37.5 41.0 34.5 41.0 28-29 38.42263716467025 40.0 38.0 41.0 34.5 41.0 30-31 38.30389792464206 40.0 37.5 41.0 34.0 41.0 32-33 37.902896929458166 40.0 36.5 41.0 33.5 41.0 34-35 37.496233555857415 40.0 35.5 41.0 33.0 41.0 36-37 37.18063314034998 39.5 35.5 41.0 32.0 41.0 38-39 36.89135788120265 39.0 35.0 41.0 32.0 41.0 40-41 36.90689582531254 40.0 35.0 41.0 31.5 41.0 42-43 36.56204644675766 40.0 35.0 41.0 30.5 41.0 44-45 36.20426330967653 39.5 35.0 41.0 30.0 41.0 46-47 35.848100070461925 39.5 35.0 41.0 26.0 41.0 48-49 35.506138178014425 39.5 35.0 41.0 23.0 41.0 50-51 35.087093118702924 39.0 35.0 41.0 20.0 41.0 52-53 35.17870814961174 40.0 35.0 41.0 18.0 41.0 54-55 35.0272041143953 40.0 35.0 41.0 16.0 41.0 56-57 34.671019082832714 39.5 35.0 41.0 13.5 41.0 58-59 34.40283155241423 39.0 35.0 41.0 10.5 41.0 60-61 33.66799721057946 38.0 33.0 40.5 10.0 41.0 62-63 33.626628069997025 38.0 33.5 40.0 10.0 41.0 64-65 33.34908072612103 37.5 33.0 39.5 10.0 41.0 66-67 32.79038666889433 37.0 33.0 39.0 10.0 41.0 68-69 32.23319265162026 36.0 32.0 39.0 9.0 40.0 70-71 31.52300545535111 35.0 31.5 38.0 8.0 39.5 72-73 31.00281847700544 35.0 30.5 37.0 8.0 39.0 74-75 30.79355019140946 35.0 31.0 37.0 8.0 39.0 76-77 30.233802837363708 34.5 30.5 36.0 7.0 37.0 78-79 30.192491809709217 35.0 30.5 36.0 7.0 37.0 80-81 29.88837959364535 35.0 29.5 35.5 7.0 36.5 82-83 29.581318146488165 34.5 29.5 35.0 7.0 36.0 84-85 29.401182598083725 34.0 29.0 35.0 7.0 36.0 86-87 29.526361476940064 35.0 30.0 35.0 7.0 35.5 88-89 29.333811554302898 35.0 29.0 35.0 7.0 35.0 90-91 29.151180782054727 34.0 29.0 35.0 7.0 35.0 92-93 29.074014077856795 34.0 29.0 35.0 7.0 35.0 94-95 28.910161045451574 34.0 29.0 35.0 7.0 35.0 96-97 28.714512977343222 34.0 28.0 35.0 7.0 35.0 98-99 28.907469690476017 34.0 29.0 35.0 7.0 35.0 100-101 28.43319192520866 34.0 27.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.047450882519143534 1101 2 0.18918178690093868 1101 3 -0.0022012563686004682 1101 4 0.008557902225092562 1101 5 0.2216747147706002 1101 6 -0.07417097140624662 1101 7 0.2991854198234236 1101 8 0.1320106637426477 1101 9 0.2540363936515959 1101 10-11 -0.027591495511174458 1101 12-13 0.32953801868837473 1101 14-15 0.30886550814216207 1101 16-17 0.23452523711227258 1101 18-19 0.3299870756001866 1101 20-21 -0.012771131325123974 1101 22-23 0.21941168716092108 1101 24-25 0.0037242840775846275 1101 26-27 0.25245175658898944 1101 28-29 -0.013755363770037832 1101 30-31 0.2736583460904214 1101 32-33 0.17266337829734368 1101 34-35 0.28843082671016873 1101 36-37 0.7739906936811849 1101 38-39 0.7705601999099443 1101 40-41 0.3885261754488667 1101 42-43 0.5760836119454424 1101 44-45 1.082655729652977 1101 46-47 0.9380745099916155 1101 48-49 1.317172473722792 1101 50-51 1.5915196445135464 1101 52-53 1.831740894441161 1101 54-55 1.407147962211333 1101 56-57 1.7893890385404703 1101 58-59 1.6875742549614117 1101 60-61 1.0594118134568404 1101 62-63 1.5188490987553465 1101 64-65 1.9878678069793452 1101 66-67 1.5167879756011189 1101 68-69 1.4533853914895047 1101 70-71 1.6680771700447146 1101 72-73 1.3677674757841949 1101 74-75 0.8989767391994157 1101 76-77 0.5837120377116811 1101 78-79 0.9391924091443116 1101 80-81 1.116737879647463 1101 82-83 1.4179926281176627 1101 84-85 1.6659408958078572 1101 86-87 1.3027955186403197 1101 88-89 1.1417620062609437 1101 90-91 1.2210230157119462 1101 92-93 0.3277944171269418 1101 94-95 1.057737295104996 1101 96-97 1.489474240192699 1101 98-99 0.7769944403148834 1101 100-101 0.7383809220771411 1102 1 0.2705690605007973 1102 2 0.3814894792086321 1102 3 0.31296968380234347 1102 4 0.31803083669802845 1102 5 0.12302799112387675 1102 6 0.098549826314553 1102 7 0.5171341377721461 1102 8 0.38770866944065574 1102 9 0.3744067640219697 1102 10-11 0.5721236042039237 1102 12-13 0.3576719218222806 1102 14-15 0.5328683571450057 1102 16-17 0.23808649067352405 1102 18-19 0.3682705513836666 1102 20-21 0.5743015182475304 1102 22-23 0.29651282676206137 1102 24-25 0.4846715775248711 1102 26-27 0.49087768251491326 1102 28-29 -0.1521100646247362 1102 30-31 0.18409281902489738 1102 32-33 0.5700992757332486 1102 34-35 0.6030675788469182 1102 36-37 0.7802228874133732 1102 38-39 0.5234092027589483 1102 40-41 0.07584811277080661 1102 42-43 0.3235907344525586 1102 44-45 0.4909534504507036 1102 46-47 -0.3941904472733455 1102 48-49 -1.3220725405222282 1102 50-51 -0.7462652557713554 1102 52-53 -1.457967082766821 1102 54-55 -1.4311711261077633 1102 56-57 -1.2440867449353163 1102 58-59 0.06595744584460306 1102 60-61 -0.13271781617279288 1102 62-63 -0.588344633438389 1102 64-65 -0.6016977200861717 1102 66-67 -0.6610966397834943 1102 68-69 -0.3325832694791515 1102 70-71 -0.7601635706960259 1102 72-73 -0.7501100170932951 1102 74-75 -0.7554036026809285 1102 76-77 -0.9812808397811956 1102 78-79 -1.4520112945593908 1102 80-81 -0.7598646844550991 1102 82-83 -1.0563663462413082 1102 84-85 -0.6221645172975556 1102 86-87 -0.46144949560469684 1102 88-89 -0.5298904153914812 1102 90-91 -0.8242049045159767 1102 92-93 -0.5559306540981304 1102 94-95 -0.6445419071742045 1102 96-97 -0.6091724834540244 1102 98-99 -0.24098989016944472 1102 100-101 -0.5144680807718593 1103 1 0.44980186473360106 1103 2 0.3547037649229168 1103 3 0.007414128246786333 1103 4 0.44468427585147197 1103 5 0.5849988905947754 1103 6 0.3928619956267241 1103 7 0.5945150901530951 1103 8 0.2947853890173704 1103 9 0.3968935365087418 1103 10-11 0.5084661967965189 1103 12-13 0.10701054616090033 1103 14-15 0.40158528836193597 1103 16-17 0.24139336898041108 1103 18-19 0.3859623503254639 1103 20-21 0.13283326427927733 1103 22-23 0.3100710278202641 1103 24-25 0.2571583500116432 1103 26-27 0.68102318516042 1103 28-29 0.40245342743875767 1103 30-31 0.5473534010354868 1103 32-33 0.796289751923716 1103 34-35 0.6229088486881835 1103 36-37 0.7726170673075501 1103 38-39 0.8608761339758857 1103 40-41 0.8926470545697427 1103 42-43 0.46859734820917964 1103 44-45 1.4549084769057288 1103 46-47 1.4044206638377688 1103 48-49 1.0664856605359745 1103 50-51 1.8507916225355245 1103 52-53 1.5051612241114896 1103 54-55 1.214496863310238 1103 56-57 2.135542884694317 1103 58-59 1.8771346945218497 1103 60-61 2.1040546705997016 1103 62-63 1.794261186667434 1103 64-65 1.8195985762101188 1103 66-67 1.3567605030736445 1103 68-69 1.3884815453356651 1103 70-71 1.6903985986161452 1103 72-73 1.265432310949027 1103 74-75 1.1592789370016128 1103 76-77 0.9583686311182724 1103 78-79 0.698120980572881 1103 80-81 1.604718648878233 1103 82-83 1.110300320425356 1103 84-85 1.1728090276759886 1103 86-87 0.9896087054535023 1103 88-89 0.8732180502169875 1103 90-91 1.2230834552723842 1103 92-93 0.5431103511928761 1103 94-95 1.1229845478522513 1103 96-97 1.5090484160168742 1103 98-99 1.0369532315236754 1103 100-101 0.7061007022969221 1104 1 0.09677988671162296 1104 2 -0.16338231566316352 1104 3 0.1569867778194336 1104 4 0.1314211500883431 1104 5 -0.04221417411829265 1104 6 0.22604270380742975 1104 7 0.25217687281487855 1104 8 0.22389100562298836 1104 9 0.2540363936515959 1104 10-11 -0.12481371773339589 1104 12-13 -0.2003765112261533 1104 14-15 -0.2509635516869011 1104 16-17 0.456747459334494 1104 18-19 0.008940067053181622 1104 20-21 0.051865620811632596 1104 22-23 0.3022108324600694 1104 24-25 0.1484892413425385 1104 26-27 0.06815688479411364 1104 28-29 0.22716343964876984 1104 30-31 0.12675663668871806 1104 32-33 0.3361249167588838 1104 34-35 0.11802484380417866 1104 36-37 0.3632000953905816 1104 38-39 0.13487643922618275 1104 40-41 0.7667313036539909 1104 42-43 0.48954515040697544 1104 44-45 0.24344632794358034 1104 46-47 0.3472625441796495 1104 48-49 0.5938818754321886 1104 50-51 0.8324384479323541 1104 52-53 0.6260785012787622 1104 54-55 0.8708231758865494 1104 56-57 0.6398163889678159 1104 58-59 0.7885357934229518 1104 60-61 1.0065271980722272 1104 62-63 1.175900380806624 1104 64-65 1.3634019950135396 1104 66-67 1.5237324200455618 1104 68-69 0.9608640239681421 1104 70-71 1.5852780247455662 1104 72-73 1.0963999544166718 1104 74-75 0.6046391323618074 1104 76-77 0.6130923795920218 1104 78-79 1.0113077937596948 1104 80-81 1.0382122386218242 1104 82-83 0.8079498930749303 1104 84-85 1.1264109812779424 1104 86-87 0.5538638947086945 1104 88-89 0.9131295276284632 1104 90-91 1.116322161011091 1104 92-93 1.2642260410585635 1104 94-95 1.5171390045067064 1104 96-97 1.580286206004665 1104 98-99 1.869408970229415 1104 100-101 1.249064682760899 1105 1 0.10001194876721797 1105 2 0.22812813467081838 1105 3 -0.12283797259355111 1105 4 0.2907977212296231 1105 5 0.333423260342677 1105 6 0.11450065108891039 1105 7 0.47791845149763645 1105 8 -0.03137007316750129 1105 9 0.2960532003742884 1105 10-11 0.05862375982172807 1105 12-13 0.2309601259928371 1105 14-15 0.07648024634512751 1105 16-17 -0.23864864782631656 1105 18-19 -0.05232546480058886 1105 20-21 0.03146771806078874 1105 22-23 0.20765506143371226 1105 24-25 0.14791465253265557 1105 26-27 -0.005426394671516732 1105 28-29 0.21075174676648345 1105 30-31 -0.11309827963678742 1105 32-33 0.32675193679767034 1105 34-35 0.5136651512091959 1105 36-37 0.3435204286520914 1105 38-39 0.4304664701103391 1105 40-41 0.5344537772588254 1105 42-43 0.7377675162764064 1105 44-45 0.4260219222838799 1105 46-47 -0.09190286557399929 1105 48-49 0.08066633280488134 1105 50-51 0.4230080090901467 1105 52-53 0.2144574425988779 1105 54-55 0.12967543473880738 1105 56-57 -0.03252434219643874 1105 58-59 0.16862629116050698 1105 60-61 0.2773739983307948 1105 62-63 0.29268555641532856 1105 64-65 0.041237231672305086 1105 66-67 0.7160042005526392 1105 68-69 0.6082819655037355 1105 70-71 0.17175364063295007 1105 72-73 0.9277222269154244 1105 74-75 0.8840688529679959 1105 76-77 0.9738623285972672 1105 78-79 1.1603058545224627 1105 80-81 0.27199806064293597 1105 82-83 0.7605104044589694 1105 84-85 0.5677670108692681 1105 86-87 1.0662893777224127 1105 88-89 0.5365583863514409 1105 90-91 1.183692698969864 1105 92-93 0.9020914436298497 1105 94-95 0.7957786654993093 1105 96-97 1.0297942143361993 1105 98-99 0.8239805424480444 1105 100-101 0.9114578451540645 1106 1 0.21185180666159198 1106 2 0.20872525156635646 1106 3 0.2391214453199595 1106 4 0.2118881086040787 1106 5 -0.16333153913809184 1106 6 0.0567702418520426 1106 7 0.037459341024174364 1106 8 0.04398689888961371 1106 9 0.3759876131637938 1106 10-11 0.318171481349367 1106 12-13 0.2751290130598605 1106 14-15 0.43112942308900415 1106 16-17 0.31238640033929244 1106 18-19 0.08721815752639372 1106 20-21 0.30160504128276244 1106 22-23 0.474922944196571 1106 24-25 0.447634540487833 1106 26-27 0.5090574476459011 1106 28-29 0.4193669001449152 1106 30-31 0.487502936459407 1106 32-33 0.38615618630235105 1106 34-35 0.24602150838622094 1106 36-37 0.5621786236374078 1106 38-39 0.2611011630118867 1106 40-41 0.34052742623060084 1106 42-43 0.6205645375239257 1106 44-45 0.6270193944434723 1106 46-47 0.06448163944752139 1106 48-49 -0.1070770572340578 1106 50-51 -0.4209490859429863 1106 52-53 -0.5401712381417028 1106 54-55 -0.8412885606154319 1106 56-57 -1.04331890392357 1106 58-59 -0.8481121115176364 1106 60-61 -0.6545109507707991 1106 62-63 -1.3062759794185155 1106 64-65 -1.1163770335459802 1106 66-67 -1.174308021897314 1106 68-69 -1.4445977229570204 1106 70-71 -1.338528521012197 1106 72-73 -1.0232581652414474 1106 74-75 -0.8404307029519309 1106 76-77 -0.8885753293927614 1106 78-79 -0.5196490542703245 1106 80-81 -0.7019378551868058 1106 82-83 -0.7281143137209831 1106 84-85 -0.4503036320220346 1106 86-87 -0.5726735245116537 1106 88-89 -0.23269077672842542 1106 90-91 -0.26943977353133164 1106 92-93 -0.45351421326705577 1106 94-95 -0.44648409326273253 1106 96-97 -0.7451255096509541 1106 98-99 -0.40065565349374666 1106 100-101 -0.486916138585773 1107 1 0.13524142517316307 1107 2 0.30072024843940426 1107 3 0.39202951286217314 1107 4 0.3297117483789407 1107 5 0.2543670224629082 1107 6 0.13544441320914302 1107 7 -0.016199195561192425 1107 8 0.0743183560503411 1107 9 0.2540363936515959 1107 10-11 -0.06028380320348248 1107 12-13 0.20261494176529737 1107 14-15 -0.018057568780918132 1107 16-17 0.4537560063430419 1107 18-19 0.4097947679078757 1107 20-21 0.34203656098257085 1107 22-23 -0.01616523591599872 1107 24-25 -0.15108340823011446 1107 26-27 -0.1638943972571596 1107 28-29 0.09297540546073435 1107 30-31 -0.05614934621726775 1107 32-33 0.016894147528113024 1107 34-35 -0.4490691732898284 1107 36-37 -0.33466315247266465 1107 38-39 -0.2544398000900472 1107 40-41 -0.09993536301266914 1107 42-43 -0.04507023420840284 1107 44-45 -0.008690424193169122 1107 46-47 0.73999758691469 1107 48-49 0.4844801660304796 1107 50-51 0.9636350291289304 1107 52-53 0.9192408944411525 1107 54-55 1.0725325775959433 1107 56-57 0.8663121154635434 1107 58-59 0.5077665626537211 1107 60-61 0.8372964288414622 1107 62-63 0.9361567910630342 1107 64-65 0.5561370377485773 1107 66-67 0.8331341294472736 1107 68-69 0.5553084684125906 1107 70-71 0.9805771700447146 1107 72-73 1.4946905527072722 1107 74-75 0.8499382776609501 1107 76-77 0.7558274223270658 1107 78-79 1.384384716836621 1107 80-81 1.0753917258013104 1107 82-83 0.7468387819638203 1107 84-85 1.064017818884782 1107 86-87 1.239333980178781 1107 88-89 1.1225312370301737 1107 90-91 0.5287153234042528 1107 92-93 1.1556790325115571 1107 94-95 0.8769680643357667 1107 96-97 0.4144742401926962 1107 98-99 0.6971867480071907 1107 100-101 1.0576116913079083 1108 1 0.10487705270352876 1108 2 0.07509850176502653 1108 3 0.05628630869791351 1108 4 -0.0961991827951465 1108 5 0.37588234924139385 1108 6 0.06673417607785126 1108 7 -0.041763105335625994 1108 8 0.20127035142338912 1108 9 0.201404814704226 1108 10-11 0.2746786028115551 1108 12-13 0.02242407999549556 1108 14-15 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78-79 -0.22147513012270537 2316 80-81 -0.5724737594987275 2316 82-83 -1.0133907790510825 2316 84-85 -1.385156566938818 2316 86-87 -0.9345685570745452 2316 88-89 -0.3488743207628211 2316 90-91 -0.5950261184000176 2316 92-93 -0.26184009757302107 2316 94-95 -0.37369645439158106 2316 96-97 -0.1779139998395216 2316 98-99 -0.3008599696730343 2316 100-101 -0.5518929401862493 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 12.0 14 48.0 15 174.0 16 425.0 17 939.0 18 1448.0 19 2024.0 20 2451.0 21 3409.0 22 2855.0 23 1893.0 24 1899.0 25 1728.0 26 1751.0 27 1805.0 28 2140.0 29 2174.0 30 2199.0 31 2700.0 32 3233.0 33 4273.0 34 5814.0 35 9505.0 36 15903.0 37 29169.0 38 33018.0 39 4667.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.74558879102351 20.457980872649053 13.288857882038313 14.50757245428912 2 14.221269795147466 29.13990992299869 32.76332994333866 23.87549033851518 3 29.606061266444385 26.144659707542438 31.703968444221676 12.545310581791503 4 10.115510352342898 16.8441699963676 44.8993824918271 28.140937159462403 5 7.672359312233498 46.61528515287332 34.01712878551245 11.695226749380733 6 22.963323478349302 41.22385826256873 15.46021806876212 20.35260019031984 7 22.588495093089648 37.89035543319555 21.811234681795398 17.7099147919194 8 24.05584652375729 24.47353319337803 20.539287971350326 30.931332311514346 9 20.880701423040325 23.070105983452343 26.263411374152824 29.78578121935451 10-11 24.95696011273908 23.75838097382739 27.863332921700096 23.421325991733436 12-13 33.232241052425124 24.97148834472589 24.55452808670449 17.241742516144498 14-15 23.72206039386037 28.0790771667042 33.439631564036816 14.759230875398618 16-17 17.31837893987491 30.035666809527616 32.263934390504346 20.382019860093127 18-19 15.43987854398059 35.91887435258566 31.08351554157617 17.55773156185758 20-21 20.894503243427792 26.50349040773483 28.838177288014933 23.763829060822445 22-23 16.798149076354232 29.390677098088787 30.06225437850953 23.748919447047456 24-25 24.059841787553662 29.019780187850042 27.94904949042226 18.971328534174035 26-27 21.737867110262016 22.915017106993165 38.06723665763495 17.279879125109872 28-29 16.26184317600558 36.23212624970937 31.24854684956987 16.257483724715183 30-31 19.327956989247312 27.149447834931706 30.026518453937808 23.496076721883174 32-33 18.500977023601113 30.418122516580343 32.83235146698822 18.24854899283032 34-35 23.103884122821675 27.824833106935053 30.82890827600735 18.242374494235925 36-37 21.319090823242266 25.45745770468463 35.312683872936084 17.910767599137024 38-39 18.142856105126288 31.30434466777566 33.547140480739195 17.00565874635886 40-41 16.974786253386895 32.7339226952776 31.038841228216736 19.252449823118774 42-43 16.24147374385274 32.867219223756564 26.48605652935066 24.405250503040033 44-45 20.906852240616576 26.17442595323362 28.798951061650552 24.11977074449925 46-47 23.973398807232154 30.61933852959764 29.52972113058701 15.877541532583193 48-49 21.248919023014817 29.835328144643803 29.272493405132003 19.643259427209372 50-51 12.810900544518669 30.776384090900834 36.21503220252171 20.197683162058784 52-53 19.02108772872885 33.90417178181501 26.15989772124681 20.914842768209326 54-55 16.4698660835897 27.79047003316129 28.22632490801646 27.51333897523255 56-57 21.984120642438416 25.195223117322733 28.32024581768522 24.500410422553628 58-59 26.863064149408338 24.78080529989903 26.86270094360867 21.493429607083968 60-61 20.014818796626543 29.49376375641966 32.706319054502664 17.785098392451133 62-63 15.880083973180884 28.69507420294487 32.265750419502695 23.159091404371544 64-65 14.845673855720129 35.57237601970028 27.61344733152699 21.9685027930526 66-67 20.2970297029703 26.768267435694415 24.775357212903977 28.159345648431316 68-69 17.10844598766553 26.999629530084334 28.476787517343077 27.415136964907056 70-71 22.929908544779643 26.783885285080235 27.430028402693534 22.85617776744659 72-73 21.117874810224972 21.179983001968576 35.14706202828647 22.55508015951999 74-75 15.557920428873409 33.20972229284557 28.413952914000127 22.81840436428089 76-77 18.63863202167612 25.541358244408446 27.583301250154364 28.23670848376107 78-79 19.144647235085806 25.40670117134296 30.413874511940435 25.0347770816308 80-81 22.779696972999282 23.620631409173633 28.888549851247237 24.71112176657985 82-83 19.170752969379972 23.52475391376993 32.85460026878791 24.449892848062184 84-85 16.65661797287579 27.062100927627615 30.796582959836705 25.484698139659894 86-87 17.707735557121378 28.525093888699217 27.764540944189797 26.002629609989615 88-89 16.30939322839107 29.086610054989357 24.10705854151079 30.496938175108777 90-91 19.319279690258092 23.404618524948607 26.122124318081113 31.153977466712192 92-93 21.94489441607404 27.85679521730603 26.541627016700204 23.65668334991973 94-95 19.230294269338895 26.15444963425176 27.832097222928454 26.78315887348089 96-97 13.830876851441564 26.83473409703406 32.555588647639524 26.77880040388485 98-99 18.21985573465637 30.001525464358615 23.803781698786167 27.974837102198848 100-101 15.187813719009466 25.64777754371182 27.483782861044727 31.680625876233993 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 272.0 1 184.0 2 111.0 3 180.5 4 160.5 5 110.5 6 203.5 7 345.0 8 436.5 9 402.0 10 262.0 11 152.0 12 119.5 13 108.5 14 127.0 15 138.5 16 127.5 17 127.5 18 138.5 19 159.5 20 177.0 21 195.5 22 217.0 23 244.5 24 294.5 25 397.5 26 534.5 27 768.5 28 1182.0 29 1350.5 30 1569.0 31 2129.5 32 2902.0 33 3723.0 34 4815.5 35 5370.0 36 4634.0 37 4746.5 38 4610.5 39 3798.5 40 3596.0 41 3084.0 42 2425.0 43 2070.0 44 1701.5 45 1461.0 46 2327.5 47 8958.0 48 21443.5 49 20691.5 50 10243.5 51 4993.0 52 1769.5 53 588.0 54 396.0 55 374.0 56 375.0 57 302.5 58 205.0 59 168.0 60 165.5 61 170.5 62 157.5 63 155.0 64 163.5 65 157.5 66 162.0 67 175.0 68 174.0 69 168.0 70 180.0 71 177.0 72 161.5 73 158.0 74 153.0 75 134.0 76 110.0 77 96.0 78 90.5 79 63.5 80 37.5 81 31.5 82 20.5 83 13.5 84 9.0 85 6.5 86 5.0 87 3.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04285828436108467 2 0.002179234798021255 3 0.0014528231986808365 4 0.009443350791425439 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014528231986808365 24-25 0.0 26-27 0.0 28-29 0.022518759579552967 30-31 0.01670746678482962 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.040679049563063424 50-51 0.013438614587797737 52-53 0.0 54-55 0.0018160289983510456 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.6320579967020914E-4 80-81 0.013438614587797737 82-83 0.005811292794723346 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 137663.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.99673841191896 #Duplication Level Percentage of deduplicated Percentage of total 1 80.28934234632636 18.46393003203475 2 8.086423652789184 3.7192273886229414 3 3.1650767578495165 2.1835932676172973 4 1.7057299892602185 1.5690490545753035 5 1.124518289215996 1.2930126468259444 6 0.7833722913639523 1.0809004598185423 7 0.4959252005812117 0.7983263476751197 8 0.45170257123002083 0.8310148696454386 9 0.3822098679638638 0.7910622316817155 >10 2.6912628719439007 12.829881667550467 >50 0.4264324973150546 6.982994704459442 >100 0.30324088697959445 12.457232517088833 >500 0.037905110872449306 5.907179125836281 >1k 0.05369890706930318 23.875696447120866 >5k 0.003158759239370775 7.216899239447056 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9935 7.216899239447056 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4627 3.3611064701481155 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3201 2.325243529488679 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2737 1.9881885473947247 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2205 1.6017375765456223 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2179 1.5828508749627717 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2135 1.550888764591793 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2012 1.4615401378729214 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1901 1.380908450346135 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1748 1.2697674756470512 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1591 1.1557208545506055 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1400 1.0169762390765855 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1279 0.929080435556395 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1277 0.9276276123577142 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1249 0.9072880875761824 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1235 0.8971183251854165 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1067 0.7750811764962262 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1025 0.7445718893239287 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 866 0.6290724450288022 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 859 0.6239875638334192 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 794 0.5767708098762921 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 746 0.5419030531079521 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 693 0.5034032383429098 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 691 0.501950415144229 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 653 0.4743467743692931 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 608 0.44165825239897427 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 591 0.4293092552101872 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 579 0.42059231601810215 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 536 0.3893566172464642 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 516 0.37482838525965584 No Hit TATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAA 426 0.3094513413190182 No Hit ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA 393 0.2854797585407844 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 366 0.2658666453585931 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 363 0.2636874105605718 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 355 0.2578761177658485 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 345 0.2506120017724443 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 336 0.24407429737838054 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 329 0.2389894161829976 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 327 0.23753659298431676 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 306 0.22228194939816798 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 301 0.2186498914014659 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 295 0.2142914218054234 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 270 0.19613113182191294 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 264 0.19177266222587042 No Hit GTCCTAGCCTTAATCTTATCTATCCTAATTTTAGCCCTAATACCTTTCCT 249 0.18087648823576413 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 244 0.17724443023906206 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 243 0.17651801863972164 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 239 0.17361237224235998 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 235 0.1707067258449983 TruSeq Adapter, Index 11 (95% over 21bp) ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA 231 0.16780107944763661 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 223 0.16198978665291328 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 222 0.16126337505357285 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 216 0.15690490545753033 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 206 0.14964078946412615 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 200 0.14528231986808365 No Hit GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC 197 0.1431030850700624 No Hit ATTCTACGCTCAATCCCCAATAAACTAGGAGGTGTCCTAGCCTTAATCTT 197 0.1431030850700624 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 189 0.13729179227533905 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 185 0.13438614587797737 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 183 0.13293332267929656 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 182 0.13220691107995614 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 180 0.1307540878812753 No Hit GTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCA 180 0.1307540878812753 No Hit GTATTAGGGCTAAAATTAGGATAGATAAGATTAAGGCTAGGACACCTCCT 179 0.13002767628193487 No Hit ATCCTAGGTATCCTAATCATATTCTTAATTCTCATAACCCTAGTATTATT 174 0.12639561828523277 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 174 0.12639561828523277 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 173 0.12566920668589235 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 169 0.1227635602885307 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 167 0.12131073708984985 No Hit GAATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTA 166 0.12058432549050943 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 164 0.1191315022918286 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 163 0.11840509069248818 No Hit CTCATAACCCTAGTATTATTTTTCCCAGACATACTAGGAGACCCAGACAA 161 0.11695226749380734 No Hit ATATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCA 161 0.11695226749380734 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 160 0.11622585589446692 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 159 0.1154994442951265 No Hit GGTTAAGATAAGTAGGTTGGCTACTAGGATTCAGTACAAAATTTGTGTGA 156 0.11332020949710525 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 156 0.11332020949710525 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 154 0.11186738629842441 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 153 0.11114097469908399 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 152 0.11041456309974357 No Hit AATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACCTTTTGCATAAT 152 0.11041456309974357 No Hit CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC 151 0.10968815150040317 No Hit TTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTT 150 0.10896173990106274 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 141 0.10242403550699897 No Hit GAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATA 139 0.10097121230831813 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 139 0.10097121230831813 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 139 0.10097121230831813 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 139 0.10097121230831813 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.264115993404183E-4 0.0 14-15 0.0 0.0 0.0 0.003268852197031882 0.0 16-17 0.0 0.0 0.0 0.02360837697856359 0.0 18-19 0.0 0.0 0.0 0.03523096256801028 0.0 20-21 0.0 0.0 0.0 0.05811292794723347 0.0 22-23 0.0 0.0 0.0 0.13765499807500928 0.0 24-25 0.0 0.0 0.0 0.24843276697442304 0.0 26-27 0.0 0.0 0.0 0.28911181653748647 0.0 28-29 0.0 0.0 0.0 0.36356900546987936 0.0 30-31 0.0 0.0 0.0 0.4609081597814954 0.0 32-33 0.0 0.0 0.0 0.6341573262241852 0.0 34-35 0.0 0.0 0.0 0.8829532989982785 0.0 36-37 0.0 0.0 0.0 1.12884362537501 0.0 38-39 0.0 0.0 0.0 1.4876909554491766 0.0 40-41 0.0 0.0 0.0 1.932981265844853 0.0 42-43 0.0 0.0 0.0 2.2725786885364987 0.0 44-45 0.0 0.0 0.0 2.608180847431772 0.0 46-47 0.0 0.0 0.0 3.0472966592330546 0.0 48-49 0.0 0.0 0.0 3.38616767032536 0.0 50-51 0.0 0.0 0.0 3.78605725576226 0.0 52-53 0.0 0.0 0.0 4.125291472654236 0.0 54-55 0.0 0.0 0.0 4.477237892534667 0.0 56-57 0.0 0.0 0.0 4.999891038260099 0.0 58-59 0.0 0.0 0.0 5.419756942678861 0.0 60-61 0.0 0.0 0.0 5.854514284884101 0.0 62-63 0.0 0.0 0.0 6.236243580337491 0.0 64-65 0.0 0.0 0.0 6.591822058214626 0.0 66-67 0.0 0.0 0.0 7.067621655782599 0.0 68-69 0.0 0.0 0.0 7.682165868824594 0.0 70-71 0.0 0.0 0.0 8.343563630024043 0.0 72-73 0.0 0.0 0.0 8.796118056413125 0.0 74-75 0.0 0.0 0.0 9.29007794396461 0.0 76-77 0.0 0.0 0.0 9.753528544343796 0.0 78-79 0.0 0.0 0.0 10.62703849255065 0.0 80-81 0.0 0.0 0.0 11.486020208770693 0.0 82-83 0.0 0.0 0.0 12.076956044834123 0.0 84-85 0.0 0.0 0.0 12.71474542905501 0.0 86-87 0.0 0.0 0.0 13.332195288494367 0.0 88-89 0.0 0.0 0.0 14.10291799539455 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATATA 20 0.0020618439 71.32255 1 TATATAA 20 0.0020707848 71.24483 5 GATATAA 20 0.0020707848 71.24483 2 ATGGGAT 25 0.0050133565 56.99586 5 GCAAAGC 25 0.0050133565 56.99586 3 ATAAGCA 25 0.0050133565 56.99586 5 CGCAGAA 110 0.0 56.132286 9 AACTTTA 110 0.0 51.81442 7 ACTTTAT 110 0.0 51.81442 8 TAACTTT 115 0.0 49.56162 6 ATGGGAG 40 5.3734897E-4 47.49655 5 AAGTACT 40 5.3734897E-4 47.49655 4 AAAGTAC 40 5.3734897E-4 47.49655 3 TGGGCAG 40 5.3734897E-4 47.49655 6 ATAACTT 120 0.0 47.496548 5 TATAACT 125 0.0 45.596687 4 CTATAAC 130 0.0 43.84297 3 ATGGGCA 55 4.942595E-5 43.17868 5 AGTACTC 45 9.598959E-4 42.21916 5 ATCTATA 140 0.0 40.75574 1 >>END_MODULE