FastQCFastQC Report
Fri 10 Feb 2017
SRR2031618_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031618_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785786
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT176022.240050089973606No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT99701.2687932846856522No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT67880.863848426925397No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT60220.7663664152835505No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC54950.6992998093628545No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC52250.6649393091757807No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC51530.6557765091258944No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT49070.6244702756221159No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA45760.5823468476149996No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA42260.5378054584836075No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41560.528897180657329No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC40700.517952725042187No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC36830.4687026747740479No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT34860.4436322357486644No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA34750.44223236351882067No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC34110.4340876523633661No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA32790.4172891856052411No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT31660.4029086799713917No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA31460.4003634577353122No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG31310.3984545410582525No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT29740.3784745465050281No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC25980.33062436846673265No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA25720.32731557955982926No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC25190.3205707406342185No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG23800.3028814460934657No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG23000.29270055714914744No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC22590.2874828515651844No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA21330.2714479514778833No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT21160.2692845125772157No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC21060.2680119014591759No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT20510.26101254030995713No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG20430.25999445141552535No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT20360.2591036236328975No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC19760.2514679569246589No Hit
TTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACATCATTCTCCA18790.2391236290796731No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC18640.2372147124026134No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT18390.23403318460751402No Hit
GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT18270.2325060512658663No Hit
GCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGT17840.22703382345829526No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA17680.22499764566943165No Hit
TTCTAGGAGTCTGCCTAATAGTCCAAATCATTACAGGTCTTTTCTTAGCC17260.21965267897366458No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG17220.2191436345264487No Hit
GTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAA16430.2090900066939345No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA16270.20705382890507085No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG16230.20654478445785493No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG16060.20438134555718734No Hit
CTTCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGT15480.19700020107255664No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA15430.19636389551353678No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG15430.19636389551353678No Hit
ATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAG14960.19038262325874986No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT14930.1900008399233379No Hit
ACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCAT14860.18911001214071008No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC14830.18872822880529813No Hit
CTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTC14800.1883464454698862No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG14580.1855467010101987No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT14130.17981995097901973No Hit
ACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAG13970.1777837731901561No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT13810.17574759540129248No Hit
TCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTC13570.172693328717997No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA12910.16429409533893452No Hit
TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA12680.16136708976744304No Hit
TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA12340.15704021196610782No Hit
CTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTCTACATTCT12240.15576760084806807No Hit
ATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAAC12150.15462225084183226No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG11910.1515679841585368No Hit
AATCCGAAGTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAG11680.14864097858704534No Hit
CTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGCCTTATAGA11610.14775015080441747No Hit
TCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACA11300.1438050563384942No Hit
GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA11200.1425324452204544No Hit
TAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCA11160.1420234007732385No Hit
GTCGTAGTAGGCAAACAATAAGGAATGTTGATCCAATAATTACATGGAGT11100.14125983410241466No Hit
GTAGTAGGCAAACAATAAGGAATGTTGATCCAATAATTACATGGAGTCCA11050.14062352854339474No Hit
ATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATT11050.14062352854339474No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC11030.14036900631978683No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA10920.13896913408994307No Hit
GAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGA10820.13769652297190327No Hit
GGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTC10320.13133346738170443No Hit
CCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAAC10290.1309516840462925No Hit
GGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGC9950.12662480624495728No Hit
AATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTAT9920.12624302290954534No Hit
CCTCCAAGCTTCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTA9860.12547945623872148No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC9760.12420684512068171No Hit
GCCTAATAGTCCAAATCATTACAGGTCTTTTCTTAGCCATACACTACACA9590.1220434062200141No Hit
ATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGT9540.12140710066099421No Hit
GTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTAT9470.12051627287836637No Hit
GTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAA9370.11924366176032661No Hit
TCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAA9350.11898913953671865No Hit
GTATATATCGGATTAGTCACCCGTAATTTACGTCTCGACAAATGTGTGTT9280.1180983117540908No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG9190.11695296174785502No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA9190.11695296174785502No Hit
ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA9040.11504404507079535No Hit
GAATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGG8960.11402595617636355No Hit
ACCCTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGT8840.11249882283471582No Hit
ATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCT8730.11109895060487207No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT8710.1108444283812641No Hit
GTTGTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAA8660.11020812282224422No Hit
GGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACATCATT8660.11020812282224422No Hit
GGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATG8570.10906277281600843No Hit
GTCCTAGCCTTAATCTTATCTATCCTAATTTTAGCCCTAATACCTTTCCT8460.10766290058616468No Hit
ATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAA8440.10740837836255672No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC8340.10613576724451695No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC8250.10499041723828115No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT8250.10499041723828115No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG8190.1042268505674573No Hit
GATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTA8150.1037178061202414No Hit
CTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGATCAACAT8120.10333602278482945No Hit
ATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAG8080.10282697833761355No Hit
GAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAA8050.10244519500220162No Hit
ATCCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACA7960.10129984499596582No Hit
GTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCT7940.10104532277235787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCTTG3750.054.480747
CCTTGGC4150.049.242159
CTATTAA4200.048.7897641
AACCCTT4200.048.643526
CTCATAA1400.047.6551171
CCCTTGG4400.046.44438
TTATGAC459.58686E-442.2546923
TCATAAC1600.041.6502762
ATAAGCC3500.040.7455983
CATAACC1700.039.147733
TATAAGC3700.038.594852
AAGCCTC3650.037.771165
ATTAACC7600.036.277883
CTCGTCC3900.035.3386579
GTGATTA1353.0559022E-1035.2122463
ATATAAG4150.034.4494861
TATTGCC1404.5292836E-1033.9468
GGGATGC851.6286609E-533.538087
TTAGCCT1605.2750693E-1132.6834534
CCTCGTC4250.032.4284138
Produced by FastQC (version 0.11.5)