FastQCFastQC Report
Fri 10 Feb 2017
SRR2031618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785786
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT198962.5319870804519296No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT93551.1905277009262063No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC68010.8655028213788487No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT64520.8210886933592607No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT54890.6985362426920306No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA50290.6399961312622011No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC49020.623833970063096No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA47600.6057628921869314No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC46620.5932913032301416No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT45590.5801834087143319No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT45200.5752202253539768No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT44180.5622395919499711No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC41440.5273700473156814No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC40400.5141348916880678No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA36520.4647575803081246No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG36180.4604307025067894No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT31620.4023996355241758No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC30760.3914551799090338No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA29980.38152881318832355No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT29010.36918448534333775No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT28840.36702104644267014No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA28370.36103977418788324No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG27800.3537858908150565No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA26120.3324060240319884No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT25980.33062436846673265No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC24270.3088627183482526No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT23420.2980455238449145No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG23350.29715469606228667No Hit
TCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTC22870.2910461626956958No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC22610.2877373737887924No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT21990.2798471848569458No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT21260.2705571236952554No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG20820.26495763477588047No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA19990.25439496249615035No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC19970.2541404402725424No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG19900.25324961248991457No Hit
GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT18550.23606936239637766No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT17870.2274156067937072No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC17780.2262702567874714No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG17650.2246158623340197No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT17590.22385229566319584No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC17570.2235977734395879No Hit
GTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAA17310.22028898453268445No Hit
TTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACATCATTCTCCA17240.21939815675005664No Hit
CTTCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGT17080.217361978961193No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG16210.20629026223424698No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC16060.20438134555718734No Hit
GTATATATCGGATTAGTCACCCGTAATTTACGTCTCGACAAATGTGTGTT15560.19801828996698848No Hit
GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA15540.19776376774338053No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC15510.19738198440796859No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA15270.19432771772467314No Hit
ACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAG15250.19407319550106517No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA15190.19330962883024133No Hit
AATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTAT14600.18580122323380666No Hit
TTCTAGGAGTCTGCCTAATAGTCCAAATCATTACAGGTCTTTTCTTAGCC14370.18287421766231518No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT14370.18287421766231518No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG14120.17969268986721576No Hit
TAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCA14100.1794381676436078No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC14070.17905638430819587No Hit
CTACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTC13930.1772747287429402No Hit
GCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGT13690.17422046205964473No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA13420.17078441204093736No Hit
ATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAAC13260.16874823425207372No Hit
CCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAAC13090.1665847953514061No Hit
TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA12890.16403957311532655No Hit
CTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTCTACATTCT12110.15411320639461634No Hit
ATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAG11920.15169524527034078No Hit
GGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGC11880.15118620082312487No Hit
ACAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGT11830.15054989526410498No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG11680.14864097858704534No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG11430.1454594507919459No Hit
ATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGT11410.14520492856833794No Hit
ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA11390.14495040634472997No Hit
ACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCAT11310.14393231745029816No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT11280.14355053411488622TruSeq Adapter, Index 11 (95% over 21bp)
AATCCGAAGTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAG11240.14304148966767033No Hit
CTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTC11170.1421506618850425No Hit
GTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCT11000.13998722298437488No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT10880.13846008964272716No Hit
GTAGTAGGCAAACAATAAGGAATGTTGATCCAATAATTACATGGAGTCCA10780.13718747852468738No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA10720.13642391185386352No Hit
GATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTA10710.13629665074205954No Hit
GAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGA10660.13566034518303965No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC10530.13400595072958796No Hit
GAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAA10220.13006085626366468No Hit
ATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATT10040.12777015625119306No Hit
GGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTC9970.12687932846856523No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG9880.12573397846232945No Hit
ACATAAATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACC9810.1248431506797016No Hit
TCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAA9770.12433410623248568No Hit
GTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTAT9660.12293423400264193No Hit
ATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAG9610.12229792844362206No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC9450.12026175065475843No Hit
GTCGTAGTAGGCAAACAATAAGGAATGTTGATCCAATAATTACATGGAGT9300.11835283397769877No Hit
GTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAA9290.1182255728658948No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT9260.11784378953048286No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC9250.11771652841867887No Hit
TATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAA9210.11720748397146295No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG9120.11606213396522717No Hit
GTGAAGTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTG9010.11466226173538342No Hit
GTCCTAGCCTTAATCTTATCTATCCTAATTTTAGCCCTAATACCTTTCCT8930.1136441728409516No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG8840.11249882283471582No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA8800.1119897783874999No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG8680.11046264504585218No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC8550.10880825059240047No Hit
ATCCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACA8450.1075356394743607No Hit
GGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATG8400.10689933391534082No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA8130.10346328389663342No Hit
TCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACA8050.10244519500220162No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC7960.10129984499596582No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT7880.10028175610153402No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGCG551.1823431E-860.45582
GGTATAC250.00502280456.9939276
AGTACTC1200.051.4528545
GGGATGC752.386514E-950.661277
CATAACC1700.047.5009883
TCATAAC1800.044.862042
AGGGGTC554.964992E-543.177225
GATGATT2150.041.972278
CTCATAA1950.041.4295731
GTATGGT704.294665E-640.733281
TTTAGAT608.287341E-539.5841562
TTACACT1850.038.5143134
GGGGTGA2350.038.4001669
AGGGGTG2350.038.4001668
GTATACA500.001611807638.0177231
GCTCCAT500.001611807638.0177231
CGAGTGT250.001615326938.00320422-23
TACTAGC756.9185025E-638.000792
CACGAGT250.001616842737.99595320-21
GAACACG250.001616842737.99595316-17
Produced by FastQC (version 0.11.5)