FastQCFastQC Report
Fri 10 Feb 2017
SRR2031617_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031617_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630147
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC21510.13195129028241012No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16870.10348759958457734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29600.038.9352231
CGTAATC702.0502906E-433.9381835
GTATCAA45050.030.4450511
ACATGGG71500.028.1093643
TACATGG74600.027.8051592
GTACATG78850.027.7827451
ATCAACG49800.027.4776653
AACGCAG50050.027.4269166
TCAACGC49950.027.3000284
CAACGCA50350.027.1775135
CATGGGG32450.026.2092764
TAGAGCG1109.460757E-525.9164355
CTTCGGC1951.5701517E-824.359899
GTCTACG1000.001629179823.8116231
CATGGGA37400.023.7567314
AGACCGT1401.7804017E-523.7494326
ACGCAGA58650.023.2432637
CGCAGAG58400.023.181528
TATCAAC60650.022.5127622
GTGTAGC7650.021.7884141
Produced by FastQC (version 0.11.5)