FastQCFastQC Report
Fri 10 Feb 2017
SRR2031617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630147
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC23780.14587641482639296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20270.12434461432005825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18100.1110329313859425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG704.311136E-640.7145234
GGTATCA31350.035.932451
GTATCAA47900.029.9672871
TACATGG66400.028.5439622
ACATGGG64200.027.820333
GTACATG70550.027.1508881
TACGTTC1056.9116344E-527.1405167
ATCAACG53400.026.4195043
TACACCG908.953667E-426.3866145
TCAACGC53500.026.3693124
AACGCAG54100.026.2500466
TACCTGG8700.026.2078532
CAACGCA54550.025.8593655
CATGGGA36650.024.3657634
CATGGGG28900.024.3254014
GGTCGTT3000.023.7479537
TATCAAC62150.022.9292372
CGCAGAG60650.022.866958
ACGCAGA61500.022.7825897
ACCTGGG10000.022.3258153
Produced by FastQC (version 0.11.5)