##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031617_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1630147 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.522904376108414 34.0 31.0 34.0 31.0 34.0 2 32.720195786024206 34.0 31.0 34.0 31.0 34.0 3 32.837853886796715 34.0 31.0 34.0 31.0 34.0 4 36.266422598698156 37.0 37.0 37.0 35.0 37.0 5 35.99011684222343 37.0 35.0 37.0 35.0 37.0 6 36.04375863035665 37.0 37.0 37.0 35.0 37.0 7 36.01590838126868 37.0 37.0 37.0 35.0 37.0 8 36.12432805139659 37.0 37.0 37.0 35.0 37.0 9 38.03128674898644 39.0 39.0 39.0 35.0 39.0 10-11 38.00419318012425 39.0 39.0 39.0 35.0 39.0 12-13 38.02359756512756 39.0 39.0 39.0 35.0 39.0 14-15 39.497943743723724 41.0 39.0 41.0 37.0 41.0 16-17 39.470760305665685 41.0 39.0 41.0 36.5 41.0 18-19 39.49710792953029 41.0 39.5 41.0 37.0 41.0 20-21 39.53079630241935 41.0 40.0 41.0 37.0 41.0 22-23 39.47597302574553 41.0 39.0 41.0 36.5 41.0 24-25 39.41607904072455 41.0 39.0 41.0 36.0 41.0 26-27 39.33325092767707 41.0 39.0 41.0 36.0 41.0 28-29 39.24902846185037 41.0 39.0 41.0 36.0 41.0 30-31 39.15101276142581 41.0 39.0 41.0 36.0 41.0 32-33 39.02581484982643 40.0 39.0 41.0 35.0 41.0 34-35 38.915901449378495 40.0 38.5 41.0 35.0 41.0 36-37 38.798468174956 40.0 38.0 41.0 35.0 41.0 38-39 38.695215830228804 40.0 38.0 41.0 35.0 41.0 40-41 38.60030506451258 40.0 38.0 41.0 35.0 41.0 42-43 38.479441117886914 40.0 38.0 41.0 34.0 41.0 44-45 38.43382038552352 40.0 38.0 41.0 34.0 41.0 46-47 38.46250184799285 40.0 38.0 41.0 34.5 41.0 48-49 38.57516101308655 40.5 39.0 41.0 35.0 41.0 50-51 38.525428688333015 41.0 39.0 41.0 34.5 41.0 52-53 38.473119602097235 40.5 38.5 41.0 34.0 41.0 54-55 38.41184476001244 40.0 38.0 41.0 34.0 41.0 56-57 38.35713343643242 40.0 38.0 41.0 34.0 41.0 58-59 38.22007463130626 40.0 38.0 41.0 34.0 41.0 60-61 38.02165602243233 40.0 38.0 41.0 33.0 41.0 62-63 37.89605630657849 40.0 37.5 41.0 33.0 41.0 64-65 37.66212341586372 40.0 37.0 41.0 33.0 41.0 66-67 37.45561933985094 39.5 36.5 41.0 33.0 41.0 68-69 37.23804478982571 39.0 36.0 41.0 32.0 41.0 70-71 36.94858807211865 39.0 36.0 41.0 32.0 41.0 72-73 36.624260879540316 38.5 35.0 40.5 31.5 41.0 74-75 36.281320028193775 37.5 35.0 40.0 31.0 41.0 76-77 35.60050842040626 37.0 35.0 39.0 30.5 40.5 78-79 35.52686843579137 37.0 35.0 39.0 31.0 41.0 80-81 35.19320834256052 36.0 35.0 39.0 31.0 39.5 82-83 34.81884455819015 36.0 35.0 37.5 31.0 39.0 84-85 34.44983274514507 35.0 34.5 37.0 30.0 39.0 86-87 34.107103837874746 35.0 34.0 37.0 30.0 38.0 88-89 33.793750195534514 35.0 34.0 36.0 29.5 37.0 90-91 33.55665225283364 35.0 34.0 36.0 29.0 37.0 92-93 33.372944587205936 35.0 34.0 36.0 29.0 36.5 94-95 33.20268693559538 35.0 34.0 35.0 29.0 36.0 96-97 33.07944375568584 35.0 34.0 35.0 29.0 36.0 98-99 32.969419322306514 35.0 34.0 35.0 29.0 36.0 100-101 32.540673939221435 35.0 33.5 35.0 27.5 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16132246441733855 1101 2 0.20895640213431932 1101 3 0.20116836538223026 1101 4 0.14000580042265653 1101 5 0.17508252162570415 1101 6 0.1510057232866444 1101 7 0.30381410001083964 1101 8 0.20665740251354237 1101 9 0.28016690624717455 1101 10-11 0.09339869478265683 1101 12-13 0.1519532327522839 1101 14-15 0.18610892224617004 1101 16-17 0.18562785958415873 1101 18-19 0.12918717036919247 1101 20-21 0.0330422174934597 1101 22-23 0.004108445829103857 1101 24-25 0.12881299756322306 1101 26-27 0.1651494694967255 1101 28-29 0.1952786776584574 1101 30-31 0.14500277558198604 1101 32-33 0.027964592633381358 1101 34-35 -0.022502157002968204 1101 36-37 0.021185739064087272 1101 38-39 0.2002714646659669 1101 40-41 0.06410511250644646 1101 42-43 -0.0496013354274254 1101 44-45 0.08281294197990974 1101 46-47 0.24145698179253117 1101 48-49 0.17709812599934338 1101 50-51 0.31841258785727433 1101 52-53 0.16856118995629288 1101 54-55 0.18849367950377172 1101 56-57 0.21424823528787584 1101 58-59 0.24445639487085202 1101 60-61 0.15179764808286933 1101 62-63 0.18092389999203817 1101 64-65 0.22163769748204487 1101 66-67 0.3259783945591792 1101 68-69 0.3238921043753322 1101 70-71 0.2523505202299532 1101 72-73 0.1694043542812551 1101 74-75 0.3406898283307811 1101 76-77 0.26461970634448306 1101 78-79 0.2756145431278867 1101 80-81 0.25613042312321 1101 82-83 0.22245520977598687 1101 84-85 0.2953509321041565 1101 86-87 0.11783856421634908 1101 88-89 0.2360874101657302 1101 90-91 0.286966739054229 1101 92-93 0.012579993794318511 1101 94-95 0.09937073651173023 1101 96-97 0.194222790379186 1101 98-99 0.18770129144413517 1101 100-101 0.20796754439043497 1102 1 0.08349342600127585 1102 2 0.07845281445543151 1102 3 0.117994867924331 1102 4 0.0961915336704422 1102 5 0.01320074698919882 1102 6 0.03337217687653293 1102 7 0.14777977235691253 1102 8 0.11719061499398009 1102 9 0.1394672041925631 1102 10-11 0.17068938333863315 1102 12-13 0.06674515190305641 1102 14-15 0.07726974372901196 1102 16-17 0.07608002820336424 1102 18-19 0.17318701647412382 1102 20-21 0.2005360701773995 1102 22-23 0.0654605272033848 1102 24-25 0.1694752442728671 1102 26-27 0.12360176032672143 1102 28-29 -0.1707163388569839 1102 30-31 -0.1203544816457125 1102 32-33 -0.05790800940414442 1102 34-35 -0.02756343779471848 1102 36-37 0.01619659658779682 1102 38-39 0.024840982804647638 1102 40-41 -0.07916243068319062 1102 42-43 0.15623926548757083 1102 44-45 0.07406100839016005 1102 46-47 0.08816532460286197 1102 48-49 0.029717567247068644 1102 50-51 0.1821020072315278 1102 52-53 -0.004961728527980824 1102 54-55 -0.24126991050616908 1102 56-57 -0.2853995599954686 1102 58-59 -0.14945982736720964 1102 60-61 0.15682073800172702 1102 62-63 0.028784475582241953 1102 64-65 0.09807975655285617 1102 66-67 0.19497945711412967 1102 68-69 0.07763480991861371 1102 70-71 0.09467568241385749 1102 72-73 0.12857350194018125 1102 74-75 0.14996800385942066 1102 76-77 0.12499444084718903 1102 78-79 -0.14184649369490643 1102 80-81 -0.11078598356262148 1102 82-83 -0.16347544238833933 1102 84-85 -0.13517251482863912 1102 86-87 -0.0785527660594525 1102 88-89 -0.021576224994312554 1102 90-91 -0.11199265867288233 1102 92-93 3.023353983735433E-4 1102 94-95 0.030987573990614692 1102 96-97 0.12905473356474317 1102 98-99 0.12872128761939194 1102 100-101 0.0791998026082581 1103 1 0.1765833391161422 1103 2 0.16502795408842275 1103 3 0.10702301372287337 1103 4 0.11069101468056886 1103 5 0.0818347398587278 1103 6 0.08152003848783096 1103 7 0.22813616689236937 1103 8 0.1120813047669813 1103 9 0.2590078712933348 1103 10-11 0.1310906173095887 1103 12-13 0.03235723759762976 1103 14-15 0.08769601385733239 1103 16-17 0.17739722019953064 1103 18-19 0.12114511905574687 1103 20-21 0.034284165602770145 1103 22-23 -0.09725354268724828 1103 24-25 -0.0649921302830947 1103 26-27 0.005079194475186455 1103 28-29 -0.019699150943964128 1103 30-31 0.006765532881708225 1103 32-33 0.04016979394705089 1103 34-35 -0.010837028194579545 1103 36-37 0.03815319914900783 1103 38-39 0.21381427470475245 1103 40-41 0.25676504612344786 1103 42-43 0.08196918229874939 1103 44-45 0.1388039203638911 1103 46-47 0.032915199422070884 1103 48-49 -0.07918595651149474 1103 50-51 -0.057510673743351504 1103 52-53 -0.008158405273583469 1103 54-55 -0.027359853707409343 1103 56-57 0.019208812181517487 1103 58-59 0.12899731137608228 1103 60-61 0.19790770480141617 1103 62-63 -0.010854316063202418 1103 64-65 0.04078993670574249 1103 66-67 0.06708633034521228 1103 68-69 -0.014589045563660363 1103 70-71 -0.08524617898682862 1103 72-73 -0.13183584460536224 1103 74-75 -0.06958324719629161 1103 76-77 -0.03014672869594648 1103 78-79 -0.018915200217413997 1103 80-81 0.036673491172344086 1103 82-83 0.04976975937300665 1103 84-85 -0.07193499583661378 1103 86-87 -0.14250644809380475 1103 88-89 -0.06373045202175831 1103 90-91 0.023706537806155836 1103 92-93 -0.05934093696707521 1103 94-95 -0.0011062546503168846 1103 96-97 0.15316197427934242 1103 98-99 -0.139355247806229 1103 100-101 -0.09304197575405482 1104 1 0.138059146790944 1104 2 0.15606339571843364 1104 3 0.20668123709109665 1104 4 0.1776327205302053 1104 5 0.11543552857403228 1104 6 0.13410611462919064 1104 7 0.085083434196072 1104 8 0.17500764077992415 1104 9 0.22141547531780503 1104 10-11 0.16829446988049312 1104 12-13 0.1567510391042859 1104 14-15 0.23813006006796655 1104 16-17 0.3317242842573691 1104 18-19 0.25122854086675517 1104 20-21 0.2519038632619015 1104 22-23 0.3195081742852963 1104 24-25 0.25219984099958026 1104 26-27 0.24105927435788743 1104 28-29 0.20154787873398305 1104 30-31 0.17518380478625062 1104 32-33 0.17777078489704934 1104 34-35 0.21970293843668998 1104 36-37 0.1479019225425091 1104 38-39 0.14680102833234088 1104 40-41 0.22878764585042433 1104 42-43 0.14722399801239305 1104 44-45 0.17714433191149226 1104 46-47 0.23815080283983292 1104 48-49 0.23730577714541568 1104 50-51 0.3371983610915734 1104 52-53 0.3392009152178517 1104 54-55 0.3725367538056119 1104 56-57 0.3240128973223264 1104 58-59 0.2704561818953195 1104 60-61 0.3632621211015348 1104 62-63 0.2766819597849164 1104 64-65 0.2164368349311374 1104 66-67 0.24760632630052015 1104 68-69 0.2005901849333256 1104 70-71 0.3624255941058365 1104 72-73 0.3385974432251686 1104 74-75 0.2046836520402735 1104 76-77 0.3058123693373176 1104 78-79 0.38530998811364015 1104 80-81 0.4121568320894795 1104 82-83 0.4347654618857675 1104 84-85 0.3653275047953599 1104 86-87 0.2721603702488835 1104 88-89 0.26268139893127085 1104 90-91 0.26528902428171364 1104 92-93 0.28461098173457344 1104 94-95 0.25610315937466055 1104 96-97 0.29255173109211796 1104 98-99 0.4139907251954398 1104 100-101 0.3727121068588204 1105 1 0.1338127189888354 1105 2 0.09258573841008655 1105 3 0.07300899372688718 1105 4 0.03511435093637516 1105 5 0.00504420237312786 1105 6 0.054455077076234204 1105 7 0.12195809014016135 1105 8 0.15255293648849744 1105 9 0.1298202526089156 1105 10-11 0.1784194070528926 1105 12-13 0.10512135096852404 1105 14-15 0.12670165959884372 1105 16-17 0.03415864437321403 1105 18-19 0.037976584235757116 1105 20-21 0.14840107491313148 1105 22-23 0.12726002321183927 1105 24-25 0.21701973520986684 1105 26-27 0.17906219434544823 1105 28-29 0.19206677488294588 1105 30-31 0.044064875129230074 1105 32-33 0.20984182197131673 1105 34-35 0.23452666213885465 1105 36-37 0.289315962417092 1105 38-39 0.3469487031127443 1105 40-41 0.17361567773094322 1105 42-43 0.06699135638102405 1105 44-45 0.03153532303185358 1105 46-47 0.1033115218269387 1105 48-49 -0.016917673705457048 1105 50-51 0.05559188925030156 1105 52-53 0.10532355469512567 1105 54-55 0.005317922279324705 1105 56-57 0.022241039300737953 1105 58-59 0.10682175476336653 1105 60-61 0.08518404733594309 1105 62-63 0.15266578018175636 1105 64-65 0.12472217994441337 1105 66-67 0.01059500536516822 1105 68-69 0.008016527050557443 1105 70-71 0.001166887995410093 1105 72-73 0.14579329780021055 1105 74-75 0.20910791187129973 1105 76-77 0.15196037919826466 1105 78-79 0.16203553321787467 1105 80-81 0.18878759726982253 1105 82-83 0.12894774569898715 1105 84-85 0.11972878767514317 1105 86-87 0.06945507358293668 1105 88-89 0.03941244893007223 1105 90-91 0.061221625224725074 1105 92-93 0.060589297236838036 1105 94-95 0.018349635045417756 1105 96-97 0.11505813352384564 1105 98-99 0.21782931211293288 1105 100-101 0.10377167939179088 1106 1 0.23653418946049243 1106 2 0.18668422310125266 1106 3 0.17212389734861944 1106 4 0.16326417175920938 1106 5 0.2113065097994351 1106 6 0.09949675867809304 1106 7 0.17425082653529245 1106 8 0.15244143041615388 1106 9 0.15876984734980226 1106 10-11 0.1811273794210564 1106 12-13 0.07321300805710251 1106 14-15 0.05886827224301072 1106 16-17 0.09486151183989477 1106 18-19 0.17383747883045686 1106 20-21 0.16569512500328187 1106 22-23 0.20503257429923138 1106 24-25 0.13076730532551295 1106 26-27 0.1535652248728212 1106 28-29 0.1960622586612928 1106 30-31 0.06073932163927509 1106 32-33 0.03452673831467479 1106 34-35 -0.007825014470064673 1106 36-37 0.09833720996689266 1106 38-39 0.14730656085988159 1106 40-41 0.02341790117105802 1106 42-43 0.09895342084146819 1106 44-45 0.06986113384352421 1106 46-47 -0.0030074866628737595 1106 48-49 0.1195921681726162 1106 50-51 0.2340472005960308 1106 52-53 0.024027276289295685 1106 54-55 0.10312251669065375 1106 56-57 0.07235128133662982 1106 58-59 0.10764405561668156 1106 60-61 0.10383174502899095 1106 62-63 0.07315879051163421 1106 64-65 0.15147393131206144 1106 66-67 0.14308114836561003 1106 68-69 0.0813924885082784 1106 70-71 0.003931742003707939 1106 72-73 0.07856168208174097 1106 74-75 0.1429863910187663 1106 76-77 0.17712125040829818 1106 78-79 0.1361203031135858 1106 80-81 0.02364010678846995 1106 82-83 0.13538733423706617 1106 84-85 0.03338606947311007 1106 86-87 0.09177953729290067 1106 88-89 0.1147684331946337 1106 90-91 0.15522621006549997 1106 92-93 0.05221347572600621 1106 94-95 0.06639656330141719 1106 96-97 -0.1419881925090749 1106 98-99 0.049819907681765585 1106 100-101 0.16009353196360365 1107 1 0.09130476082603423 1107 2 0.04963964403152232 1107 3 0.022862073798251004 1107 4 0.05498089866785705 1107 5 -5.361313449938621E-4 1107 6 0.051683712469944965 1107 7 0.10496872434452342 1107 8 0.13171479327655078 1107 9 0.1694046790964805 1107 10-11 0.15492402453636345 1107 12-13 0.09994941967108417 1107 14-15 -0.0035568495136857337 1107 16-17 0.047426038643337165 1107 18-19 -9.711892277124434E-4 1107 20-21 0.03999428161547769 1107 22-23 0.08591168305722618 1107 24-25 0.0943590852640881 1107 26-27 0.02859301254952129 1107 28-29 0.05336299344383377 1107 30-31 0.08248137563645486 1107 32-33 0.132979961685173 1107 34-35 0.023818997815901355 1107 36-37 0.10071231390552526 1107 38-39 0.1256741727317987 1107 40-41 0.11577819915677168 1107 42-43 -0.087678161231878 1107 44-45 0.030396693584911816 1107 46-47 0.23004351539044166 1107 48-49 0.15524099981747952 1107 50-51 0.10253236918873654 1107 52-53 0.0022845859331042107 1107 54-55 0.16341732060723047 1107 56-57 0.14959656803757326 1107 58-59 0.06573620888587328 1107 60-61 0.1398579375657718 1107 62-63 0.04101056977142292 1107 64-65 -0.051586107039177875 1107 66-67 0.16399296597663238 1107 68-69 0.20771569100620013 1107 70-71 0.2763763363460541 1107 72-73 0.18488624214553795 1107 74-75 0.2334454604073528 1107 76-77 0.07330224787983042 1107 78-79 0.030748584604872065 1107 80-81 0.09179245961983895 1107 82-83 0.1555380664741648 1107 84-85 0.17972021734597377 1107 86-87 0.06339856188181159 1107 88-89 -0.10690362131163766 1107 90-91 0.05540036031410267 1107 92-93 0.2017837212757314 1107 94-95 0.2623211080926069 1107 96-97 0.03988978777228169 1107 98-99 0.18349814759759653 1107 100-101 0.2659813376280482 1108 1 -0.015751275432720035 1108 2 0.029706836830484917 1108 3 0.017589657710011863 1108 4 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-0.7443337189044357 2316 66-67 -0.7539799924375004 2316 68-69 -0.8522713807455702 2316 70-71 -0.8439973259393696 2316 72-73 -1.0197715183945775 2316 74-75 -0.9125997525496246 2316 76-77 -0.8594959996243006 2316 78-79 -0.8450217235183786 2316 80-81 -1.1464141050573105 2316 82-83 -1.4828830712116812 2316 84-85 -1.3896452211371155 2316 86-87 -0.9656729385132152 2316 88-89 -0.8272665100568801 2316 90-91 -0.6481863307725959 2316 92-93 -0.6326036234057284 2316 94-95 -0.680432788337761 2316 96-97 -0.6210282974105041 2316 98-99 -0.7710321260503221 2316 100-101 -0.9240666046257786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 2.0 13 20.0 14 84.0 15 295.0 16 738.0 17 1480.0 18 2331.0 19 3276.0 20 4432.0 21 5772.0 22 6128.0 23 6096.0 24 5832.0 25 6373.0 26 7352.0 27 8889.0 28 11553.0 29 14432.0 30 17819.0 31 22561.0 32 28714.0 33 37441.0 34 51572.0 35 76893.0 36 128040.0 37 271078.0 38 561364.0 39 348599.0 40 980.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.2774436348639 22.945900762009504 15.461931438783926 21.314724164342667 2 13.036876994482622 22.80203567585739 44.710763979628524 19.45032335003147 3 17.113746477283463 28.992156473026142 32.342494543393364 21.55160250629703 4 10.009196482636808 19.35506828035591 43.769367675866626 26.86636756114066 5 10.358575024215607 40.55940967287 38.07497115290829 11.007044150006104 6 25.240975200396036 42.643516198232426 20.642310172027432 11.473198429344102 7 23.831961166692327 34.70913972788957 25.427400105634646 16.031498999783455 8 21.689639032553508 40.58118685002027 22.8130346527031 14.916139464723118 9 24.957381144154482 17.363526111448845 23.04282987975931 34.63626286463736 10-11 21.943082433670092 29.38541738873856 31.40701421405554 17.264485963535805 12-13 22.032430204147232 26.704677553619398 31.906662405292284 19.356229836941086 14-15 19.497566783854463 28.909110650757263 29.22868305741752 22.364639507970754 16-17 18.68500202742452 32.45937329578253 29.883071894743235 18.972552782049718 18-19 18.689510823257045 31.467008803500544 31.586905966149065 18.25657440709335 20-21 19.789288941426754 30.475993882760267 31.407412951101954 18.32730422471102 22-23 19.298626600806323 30.378482267519612 31.375681239862686 18.947209891811383 24-25 19.0081630675025 30.386247375236714 31.26469576056638 19.34089379669441 26-27 19.14054376691182 30.585922619248446 31.18681934819375 19.08671426564598 28-29 19.04477472743064 30.43886282764082 31.490362860313244 19.025999584615306 30-31 19.424175287492076 30.69601827383885 30.80234570711424 19.07746073155483 32-33 19.041545971955887 30.540669363357637 31.079056722526694 19.33872794215978 34-35 18.872653815882863 30.819459840124846 31.17866057478252 19.12922576920977 36-37 19.099075114084805 30.377628520618078 31.12691677499023 19.396379590306886 38-39 19.206642100374996 30.30907642071543 31.36622034699938 19.11806113191019 40-41 19.083555041355165 30.241659187791043 31.445047593867304 19.229738176986494 42-43 19.28102189557138 30.16905837326327 31.132315061157062 19.417604670008288 44-45 19.427020998719748 30.15040974832331 30.79495284781066 19.627616405146284 46-47 19.173056172234773 30.03913144029342 30.966869858975908 19.820942528495898 48-49 19.155780399318594 30.003326018547156 31.197563047074823 19.643330535059427 50-51 19.102011467808293 30.270424809534504 30.803691392517823 19.823872330139377 52-53 19.214371464659322 30.054160759735165 30.903102603630224 19.82836517197529 54-55 19.224697303141607 30.053998512395424 30.811449954375014 19.90985423008795 56-57 19.289180668982613 29.988430491237906 30.54801192775866 20.174376912020815 58-59 19.23559654436072 29.949476949011345 30.696004716139097 20.118921790488834 60-61 19.276678285257884 30.304279493983977 30.462394165921335 19.956648054836805 62-63 19.092327256376265 30.097040328264875 30.604264523383474 20.206367891975386 64-65 19.119594735934857 29.897917181702017 30.597363305272467 20.385124777090656 66-67 19.38917165139095 29.89024916157868 30.24073902537624 20.479840161654135 68-69 19.317828392163406 30.01809039307498 30.071398468972433 20.592682745789183 70-71 19.282678187918023 29.459061054003104 30.469798122500606 20.788462635578263 72-73 19.24470615226725 29.70753557807977 30.271963203318474 20.77579506633451 74-75 19.083708401757633 29.777743970329055 30.063055663078238 21.075491964835074 76-77 18.86105976945637 29.677937020403682 30.403546428634964 21.05745678150498 78-79 19.198831268535564 29.889669222372138 29.80016789932911 21.11133160976319 80-81 19.283077949996564 29.571316737506503 29.862886878760808 21.28271843373612 82-83 19.147626990646362 29.424880418585563 29.997036920704197 21.430455670063882 84-85 19.283076924964433 29.346709223155948 29.961745781208688 21.408468070670928 86-87 18.715766124159355 29.603649241448775 30.14050266632396 21.540081968067913 88-89 19.26786357303973 29.16056036664178 29.842768780974964 21.72880727934352 90-91 19.251546026217266 29.502400703740218 29.649902738832758 21.596150531209762 92-93 19.266237952773587 29.15733979818998 29.781792684954176 21.794629564082257 94-95 19.130360636188023 29.020787695833565 29.75096724405836 22.09788442392005 96-97 18.996108939868613 29.04734971754081 29.924939284616663 22.031602057973913 98-99 19.077389953176002 28.803721382182097 29.80402994331186 22.31485872133004 100-101 19.161891534935194 28.80902765210745 29.651620375340382 22.377460437616975 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 52.0 1 38.0 2 43.5 3 83.0 4 74.5 5 49.5 6 80.5 7 173.5 8 278.5 9 371.0 10 365.5 11 307.5 12 338.5 13 386.0 14 430.0 15 474.0 16 532.5 17 584.0 18 647.5 19 745.5 20 848.5 21 980.5 22 1146.5 23 1401.0 24 1723.0 25 2302.0 26 3646.5 27 6210.5 28 11405.0 29 21850.5 30 35584.5 31 51410.5 32 69595.5 33 86777.5 34 99773.5 35 105278.5 36 109965.0 37 112426.0 38 105609.5 39 94038.0 40 81983.5 41 70580.0 42 59749.5 43 50698.5 44 43604.5 45 38164.0 46 33971.0 47 31426.0 48 30254.0 49 29324.0 50 27910.0 51 25771.0 52 23674.5 53 22002.5 54 20528.0 55 19281.5 56 17770.0 57 15617.5 58 13597.0 59 11516.5 60 9307.5 61 7328.0 62 5452.5 63 4022.5 64 2869.5 65 1898.0 66 1247.5 67 832.5 68 526.5 69 394.5 70 308.0 71 197.5 72 102.5 73 54.5 74 50.0 75 47.0 76 31.5 77 12.5 78 9.5 79 11.0 80 6.5 81 3.5 82 3.5 83 3.0 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0472963481207523 2 0.0015336040246677139 3 7.974740928272113E-4 4 0.010796572333660708 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0019016689905879654 24-25 0.0 26-27 0.0 28-29 0.020396933528080596 30-31 0.016930988432331564 32-33 6.134416098670856E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.035027515923410586 50-51 0.011839423070434752 52-53 0.0 54-55 5.827695293737313E-4 56-57 0.0 58-59 0.0 60-61 6.134416098670856E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.1470456345347997E-4 80-81 0.010980604816620833 82-83 0.005275597844856936 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1630147.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.60264367279486 #Duplication Level Percentage of deduplicated Percentage of total 1 63.345564236311304 23.819606802301518 2 15.755760317174369 11.849164820013383 3 7.017536871479443 7.916338153168234 4 3.8512594795258357 5.792701516003336 5 2.3899733308613156 4.493465777393036 6 1.5682336497254197 3.5381838675786934 7 1.1029437533450839 2.9031520663719097 8 0.8015777972821162 2.4113155429778566 9 0.5939346980107402 2.01001633327862 >10 3.2743706438520204 22.06858434033311 >50 0.20145617752717637 5.133528756308277 >100 0.0912609094714169 6.107307455943306 >500 0.005134383741850404 1.330289791498303 >1k 9.93751691971046E-4 0.6263447768304511 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 2378 0.14587641482639296 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2027 0.12434461432005825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1810 0.1110329313859425 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 6.134416098670856E-5 0.0 0.0 0.0 5 0.0 6.134416098670856E-5 0.0 0.0 0.0 6 0.0 6.134416098670856E-5 0.0 0.0 0.0 7 0.0 6.134416098670856E-5 0.0 0.0 0.0 8 0.0 6.134416098670856E-5 0.0 6.134416098670856E-5 0.0 9 0.0 6.134416098670856E-5 0.0 6.134416098670856E-5 0.0 10-11 0.0 6.134416098670856E-5 0.0 5.827695293737314E-4 0.0 12-13 0.0 6.134416098670856E-5 0.0 0.0010735228172673997 0.0 14-15 0.0 6.134416098670856E-5 0.0 0.001533604024667714 0.0 16-17 0.0 6.134416098670856E-5 0.0 0.0038953542226559936 0.0 18-19 0.0 6.134416098670856E-5 0.0 0.006011727776697439 0.0 20-21 0.0 6.134416098670856E-5 0.0 0.010121786562806913 0.0 22-23 0.0 6.134416098670856E-5 0.0 0.019415426952293258 0.0 24-25 0.0 6.134416098670856E-5 0.0 0.029935950561513776 0.0 26-27 0.0 6.134416098670856E-5 0.0 0.037143889477452036 0.0 28-29 0.0 6.134416098670856E-5 0.0 0.04536400704967098 0.0 30-31 0.0 6.134416098670856E-5 0.0 0.06873613238560694 0.0 32-33 0.0 6.134416098670856E-5 0.0 0.11833288654336081 0.0 34-35 0.0 6.134416098670856E-5 0.0 0.18832657422919527 0.0 36-37 0.0 6.134416098670856E-5 0.0 0.29512675850705483 0.0 38-39 0.0 6.134416098670856E-5 0.0 0.4663996559819452 0.0 40-41 0.0 6.134416098670856E-5 0.0 0.6675778319378559 0.0 42-43 0.0 6.134416098670856E-5 0.0 0.8531745910031427 0.0 44-45 0.0 6.134416098670856E-5 0.0 1.057174598364442 0.0 46-47 0.0 6.134416098670856E-5 0.0 1.3021525052648626 0.0 48-49 0.0 6.134416098670856E-5 0.0 1.5550131368520752 0.0 50-51 0.0 6.134416098670856E-5 0.0 1.8339143647781457 0.0 52-53 0.0 6.134416098670856E-5 0.0 2.092970756625016 0.0 54-55 0.0 6.134416098670856E-5 0.0 2.358222908731544 0.0 56-57 0.0 6.134416098670856E-5 0.0 2.677672627069829 0.0 58-59 0.0 6.134416098670856E-5 0.0 3.018194064707048 0.0 60-61 0.0 6.134416098670856E-5 0.0 3.365279327569845 0.0 62-63 0.0 6.134416098670856E-5 0.0 3.7075797458756785 0.0 64-65 0.0 6.134416098670856E-5 0.0 4.037212594937757 0.0 66-67 0.0 6.134416098670856E-5 0.0 4.408712833873264 0.0 68-69 0.0 6.134416098670856E-5 0.0 4.845023178891228 0.0 70-71 0.0 6.134416098670856E-5 0.0 5.314213994198068 0.0 72-73 0.0 6.134416098670856E-5 0.0 5.779816176087188 0.0 74-75 0.0 6.134416098670856E-5 0.0 6.207078257359612 0.0 76-77 0.0 6.134416098670856E-5 0.0 6.672281702202317 0.0 78-79 0.0 6.134416098670856E-5 0.0 7.215453575659128 0.0 80-81 0.0 6.134416098670856E-5 0.0 7.828159055594373 0.0 82-83 0.0 6.134416098670856E-5 0.0 8.461537517782139 0.0 84-85 0.0 6.134416098670856E-5 0.0 9.03397055602961 0.0 86-87 0.0 6.134416098670856E-5 0.0 9.591343602754844 0.0 88-89 0.0 6.134416098670856E-5 0.0 10.23104051352424 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTACG 70 4.311136E-6 40.714523 4 GGTATCA 3135 0.0 35.93245 1 GTATCAA 4790 0.0 29.967287 1 TACATGG 6640 0.0 28.543962 2 ACATGGG 6420 0.0 27.82033 3 GTACATG 7055 0.0 27.150888 1 TACGTTC 105 6.9116344E-5 27.140516 7 ATCAACG 5340 0.0 26.419504 3 TACACCG 90 8.953667E-4 26.386614 5 TCAACGC 5350 0.0 26.369312 4 AACGCAG 5410 0.0 26.250046 6 TACCTGG 870 0.0 26.207853 2 CAACGCA 5455 0.0 25.859365 5 CATGGGA 3665 0.0 24.365763 4 CATGGGG 2890 0.0 24.325401 4 GGTCGTT 300 0.0 23.747953 7 TATCAAC 6215 0.0 22.929237 2 CGCAGAG 6065 0.0 22.86695 8 ACGCAGA 6150 0.0 22.782589 7 ACCTGGG 1000 0.0 22.325815 3 >>END_MODULE