FastQCFastQC Report
Fri 10 Feb 2017
SRR2031616_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031616_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921648
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19000.033.595431
GTATCAA31100.026.9576051
TCAACGC27350.026.9406194
CAACGCA29000.025.5717125
ATCAACG29300.025.4701353
AACGCAG30750.024.4160446
GTACATG46000.024.1283231
ACATGGG43650.022.7594243
CGCAGAG34600.021.1504338
ACGCAGA35850.020.9420857
GTATAGG10900.020.5399971
TACATGG49100.020.3544042
TATCAAC37050.020.2950172
CATGGGG22900.019.928244
ACGACTA1200.00474313719.8071465
GACCGTC1200.004754599619.7988877
GTATAGC6750.019.7598321
GTACGAC1300.00753428818.2820913
GAGTACA36700.018.0417121
GTCGCCG6750.017.94397294-95
Produced by FastQC (version 0.11.5)