##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031616_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1921648 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13536714320208 34.0 31.0 34.0 30.0 34.0 2 32.36493780338543 34.0 31.0 34.0 31.0 34.0 3 32.517705115609104 34.0 31.0 34.0 31.0 34.0 4 35.60024572658468 37.0 35.0 37.0 33.0 37.0 5 35.33763727800305 37.0 35.0 37.0 33.0 37.0 6 35.54790419473285 37.0 35.0 37.0 33.0 37.0 7 35.69212259477282 37.0 36.0 37.0 33.0 37.0 8 35.7682437158106 37.0 36.0 37.0 35.0 37.0 9 37.72803656028575 39.0 38.0 39.0 35.0 39.0 10-11 37.68438912849804 39.0 38.0 39.0 35.0 39.0 12-13 37.7128485549903 39.0 38.0 39.0 35.0 39.0 14-15 39.19644778856481 41.0 39.0 41.0 36.0 41.0 16-17 39.17146376443553 41.0 39.0 41.0 36.0 41.0 18-19 39.09482433827631 41.0 39.0 41.0 36.0 41.0 20-21 39.10974980849771 41.0 39.0 41.0 36.0 41.0 22-23 39.063582664463 41.0 39.0 41.0 36.0 41.0 24-25 39.01374653422479 41.0 39.0 41.0 36.0 41.0 26-27 38.949213123319154 41.0 39.0 41.0 35.5 41.0 28-29 38.862662933065785 41.0 39.0 41.0 35.0 41.0 30-31 38.773135610684164 40.0 39.0 41.0 35.0 41.0 32-33 38.666771177655846 40.0 38.0 41.0 35.0 41.0 34-35 38.570654719282615 40.0 38.0 41.0 34.0 41.0 36-37 38.48489525657144 40.0 38.0 41.0 34.0 41.0 38-39 38.35248052713088 40.0 38.0 41.0 34.0 41.0 40-41 38.24622485491619 40.0 38.0 41.0 34.0 41.0 42-43 38.1236186335895 40.0 38.0 41.0 33.0 41.0 44-45 37.97682275838239 40.0 38.0 41.0 33.0 41.0 46-47 37.85549772903258 40.0 38.0 41.0 33.0 41.0 48-49 38.02133923590585 40.0 38.0 41.0 33.0 41.0 50-51 37.81682207147199 39.5 37.5 40.5 33.0 41.0 52-53 37.97537166015836 40.0 38.0 41.0 33.5 41.0 54-55 38.294357499396355 40.0 38.0 41.0 34.0 41.0 56-57 38.24569952457474 40.0 38.0 41.0 34.0 41.0 58-59 38.11164219461629 40.0 38.0 41.0 33.0 41.0 60-61 37.9117663588753 40.0 37.5 41.0 33.0 41.0 62-63 37.671582932982524 40.0 37.0 41.0 32.5 41.0 64-65 37.47595423303331 40.0 37.0 41.0 32.0 41.0 66-67 37.329516644047196 39.5 36.0 41.0 32.0 41.0 68-69 37.139541164666994 39.0 36.0 41.0 32.0 41.0 70-71 36.87973369732646 39.0 35.5 41.0 31.5 41.0 72-73 36.54224342855716 38.5 35.0 40.5 31.0 41.0 74-75 36.11585394411463 37.5 35.0 40.0 31.0 41.0 76-77 35.68275199204017 37.0 35.0 39.0 30.5 41.0 78-79 35.256784801378814 36.5 35.0 39.0 30.0 40.5 80-81 34.83220886447466 36.0 35.0 38.0 30.0 39.0 82-83 34.41880328759481 35.5 34.0 37.0 29.5 39.0 84-85 34.053168166074116 35.0 34.0 37.0 29.0 39.0 86-87 33.723728799447144 35.0 34.0 36.5 29.0 37.0 88-89 33.40427695394786 35.0 34.0 36.0 29.0 37.0 90-91 33.15202003696827 35.0 34.0 36.0 29.0 37.0 92-93 32.93040634913366 35.0 34.0 35.0 28.0 36.0 94-95 32.738066753120236 35.0 34.0 35.0 27.0 36.0 96-97 32.606071455334174 35.0 34.0 35.0 27.0 36.0 98-99 32.47992478331099 35.0 34.0 35.0 26.0 36.0 100-101 32.010517795142505 34.5 32.5 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25668477799808187 1101 2 0.19545928413555913 1101 3 0.24422379819227302 1101 4 0.09245166006892447 1101 5 0.04262996310705347 1101 6 0.08979157095767931 1101 7 0.17162571339820687 1101 8 0.17467781231533763 1101 9 0.16291284858920108 1101 10-11 0.29099786682119344 1101 12-13 0.26102991027241984 1101 14-15 0.3018539726278888 1101 16-17 0.34159568456414746 1101 18-19 0.4509019877340634 1101 20-21 0.1557846882893088 1101 22-23 0.23024999937565838 1101 24-25 0.3429372614391326 1101 26-27 0.29665782488457637 1101 28-29 0.29574163084371463 1101 30-31 0.267311087780314 1101 32-33 0.4407854470671708 1101 34-35 0.24610650690825508 1101 36-37 0.18560608681221424 1101 38-39 0.2586624212419437 1101 40-41 0.21047723199944102 1101 42-43 0.20906405776868553 1101 44-45 0.21450463797818742 1101 46-47 0.2713886940828729 1101 48-49 0.2339430901076156 1101 50-51 0.07471949192606786 1101 52-53 0.0648589369464787 1101 54-55 0.11064473438410971 1101 56-57 0.1734584399528174 1101 58-59 0.17790247465861597 1101 60-61 0.14043519695338347 1101 62-63 -0.04484471120171207 1101 64-65 0.12260227750878983 1101 66-67 0.14981558280732088 1101 68-69 0.07589974682201017 1101 70-71 0.19942541690726756 1101 72-73 0.16918344423589105 1101 74-75 0.046556941496199045 1101 76-77 0.1231192436580173 1101 78-79 0.19317228822232835 1101 80-81 0.10876796997442284 1101 82-83 0.08149554474675114 1101 84-85 0.08717865979796358 1101 86-87 0.17465283709702106 1101 88-89 0.22626862429536487 1101 90-91 0.04700241088843882 1101 92-93 -0.06489709057136395 1101 94-95 -0.0254070310023522 1101 96-97 0.01803453783554687 1101 98-99 -0.047387188037625094 1101 100-101 0.057633258689676836 1102 1 0.051056044033522596 1102 2 0.17495498186798386 1102 3 0.2173731885060448 1102 4 0.2586980307743687 1102 5 0.27378936295791334 1102 6 0.084923424308613 1102 7 0.12975110232989095 1102 8 0.15145076787884193 1102 9 0.25618197256960684 1102 10-11 0.2882427320414891 1102 12-13 0.3067616659607424 1102 14-15 0.22523332844242105 1102 16-17 0.28372331056919364 1102 18-19 0.40814087684820777 1102 20-21 0.23828448115288836 1102 22-23 0.14231706221644203 1102 24-25 0.31205167831043923 1102 26-27 0.24863104170439954 1102 28-29 0.199368325175584 1102 30-31 0.24216935358129632 1102 32-33 0.12770403275730047 1102 34-35 0.08167257990135823 1102 36-37 0.11250037143352642 1102 38-39 0.018167999822445324 1102 40-41 0.1988044335070427 1102 42-43 0.05509426619200042 1102 44-45 -0.2062238123416833 1102 46-47 -0.661219691152084 1102 48-49 0.05213923155270095 1102 50-51 0.0429313228533843 1102 52-53 0.16790578150954616 1102 54-55 0.19193299953283827 1102 56-57 0.25190232537377 1102 58-59 0.18400021837004488 1102 60-61 -0.047697120030569806 1102 62-63 -0.4350809172381034 1102 64-65 -0.10438530664588797 1102 66-67 0.062483561631808016 1102 68-69 0.2724384971988201 1102 70-71 0.15034201761347532 1102 72-73 0.23027017886681023 1102 74-75 0.14739139277858726 1102 76-77 0.09023325154506523 1102 78-79 0.09035263265053572 1102 80-81 -0.0059331630177439365 1102 82-83 -0.09791500669448538 1102 84-85 -0.0033271761605675465 1102 86-87 0.09227754644495434 1102 88-89 -0.12091097091599323 1102 90-91 -0.04462438568948812 1102 92-93 0.05988497546901783 1102 94-95 -0.040919565840937366 1102 96-97 -0.004155502340097428 1102 98-99 -0.15868863940287525 1102 100-101 -0.11075895721170426 1103 1 0.026778839742597427 1103 2 -0.05764600388511809 1103 3 -0.041525222287347674 1103 4 0.17810411790092218 1103 5 0.04486514525616059 1103 6 0.1381284703935819 1103 7 0.1774420498530347 1103 8 0.13359874209553624 1103 9 0.3287974041231436 1103 10-11 0.3170437306053344 1103 12-13 0.19764677886385584 1103 14-15 0.2643195852102096 1103 16-17 0.28939627922332534 1103 18-19 0.2507594015223731 1103 20-21 0.038554855294066215 1103 22-23 0.12291089080867579 1103 24-25 0.11047397608609089 1103 26-27 0.007003748412515165 1103 28-29 0.0318008327777477 1103 30-31 0.2855451028413043 1103 32-33 0.12208268634802266 1103 34-35 0.0870855410038942 1103 36-37 0.1332442964434719 1103 38-39 0.04916663109549546 1103 40-41 -0.02229258765765252 1103 42-43 -0.045804887961715224 1103 44-45 -0.09431521363489281 1103 46-47 0.0843732440592575 1103 48-49 0.037193222099723755 1103 50-51 -0.039406789786774254 1103 52-53 -0.05172917125926091 1103 54-55 0.02025532292997667 1103 56-57 0.023930485413679037 1103 58-59 -0.13245699542780898 1103 60-61 -0.03757873863446548 1103 62-63 0.022435660877093255 1103 64-65 0.09254281159054045 1103 66-67 -0.14193821022905695 1103 68-69 -0.1970451707871277 1103 70-71 -0.06370171928080026 1103 72-73 -0.02493439692897681 1103 74-75 -0.052240888275669306 1103 76-77 -0.6493280984377421 1103 78-79 -0.3284466297202613 1103 80-81 -0.21971436392392718 1103 82-83 -0.19797429852999215 1103 84-85 -0.23170124822437543 1103 86-87 -0.3023979034481812 1103 88-89 -0.1858298418174016 1103 90-91 -0.18528025368487988 1103 92-93 -0.3208558395640466 1103 94-95 -0.33577171130357186 1103 96-97 -0.15091796584633244 1103 98-99 -0.21667748731236713 1103 100-101 -0.13662612664391816 1104 1 0.03279444728784142 1104 2 0.19935345352128309 1104 3 0.15384677349957343 1104 4 0.17505202891557303 1104 5 0.03574997178579764 1104 6 0.02799473703046118 1104 7 -0.05599647748410774 1104 8 0.15816107652469213 1104 9 0.256104623809712 1104 10-11 0.27918705510822406 1104 12-13 0.23119881199398407 1104 14-15 0.29425798487826427 1104 16-17 0.21856467474214014 1104 18-19 0.3296910107911515 1104 20-21 0.2100623079767061 1104 22-23 0.23488186205121764 1104 24-25 0.24411577653931005 1104 26-27 0.25600812905787507 1104 28-29 0.26185134185821113 1104 30-31 0.31621560489997336 1104 32-33 0.28505246867223377 1104 34-35 0.2938539971655274 1104 36-37 0.29671717474291626 1104 38-39 0.2078018486116946 1104 40-41 0.18469051214764676 1104 42-43 0.10977963694971749 1104 44-45 0.18348313305049402 1104 46-47 0.4045840576304798 1104 48-49 0.2470544617665098 1104 50-51 0.1650787836069867 1104 52-53 0.15738979238696515 1104 54-55 0.11164863147423176 1104 56-57 0.13361298836603197 1104 58-59 0.031013888042245696 1104 60-61 0.0988911314622456 1104 62-63 0.31650483358144044 1104 64-65 0.32276208323842326 1104 66-67 0.13121198446629023 1104 68-69 -0.007691375823675628 1104 70-71 0.013517528429140668 1104 72-73 0.10273847498701372 1104 74-75 0.10990802174954695 1104 76-77 0.19461520053133796 1104 78-79 0.1403869536316904 1104 80-81 0.18633216347704717 1104 82-83 0.15525193906216828 1104 84-85 0.14412082097226175 1104 86-87 0.13272386093853328 1104 88-89 0.24363737914585926 1104 90-91 0.27397515629246527 1104 92-93 0.0864097280846039 1104 94-95 0.028282061317796092 1104 96-97 0.13709689962237093 1104 98-99 0.14252585761757786 1104 100-101 -0.004998904403969107 1105 1 0.15557651017726215 1105 2 0.19475171689860815 1105 3 0.10828781075269234 1105 4 0.21106970861213625 1105 5 -0.01816904605801284 1105 6 0.06283819574557015 1105 7 0.2263524070244003 1105 8 0.05939411919162296 1105 9 0.2270205272388921 1105 10-11 0.2659306017792673 1105 12-13 0.20266384075022614 1105 14-15 0.21202102752675245 1105 16-17 0.2695628916521784 1105 18-19 0.3142209075120732 1105 20-21 0.16855982879940257 1105 22-23 0.23246500991492525 1105 24-25 0.2915350903354863 1105 26-27 0.2742976165716442 1105 28-29 0.32847695012790723 1105 30-31 0.2876124078768285 1105 32-33 0.2847165028072567 1105 34-35 0.3179839801828379 1105 36-37 0.27015595685743676 1105 38-39 0.3028737346119428 1105 40-41 0.26645892434937224 1105 42-43 0.317470341832653 1105 44-45 0.3308795819908781 1105 46-47 0.4598030531626165 1105 48-49 0.3536144626515991 1105 50-51 0.14651749933967295 1105 52-53 0.185915543930804 1105 54-55 0.4114719820238193 1105 56-57 0.4765613008843488 1105 58-59 0.41250736879242567 1105 60-61 0.4989807469454419 1105 62-63 0.4709930756981393 1105 64-65 0.45795423100440047 1105 66-67 0.38132271306230336 1105 68-69 0.418286235057046 1105 70-71 0.22818643978541076 1105 72-73 0.21141513457910577 1105 74-75 0.35433664928657294 1105 76-77 0.3765091850459257 1105 78-79 0.27064969663993566 1105 80-81 0.3272681842914409 1105 82-83 0.1946296461965531 1105 84-85 0.3335585483531176 1105 86-87 0.2666969799814538 1105 88-89 0.32158551648988265 1105 90-91 0.3015654770827041 1105 92-93 0.20585794748395614 1105 94-95 0.05790790745575691 1105 96-97 0.16403981308442184 1105 98-99 0.09747305639614723 1105 100-101 0.04311428400797723 1106 1 0.019001833273456725 1106 2 -0.06003427080385393 1106 3 0.06364123267971422 1106 4 0.20792791490561768 1106 5 0.09774044434339402 1106 6 0.0244782769099956 1106 7 0.07572174441481394 1106 8 0.13491731061235868 1106 9 0.1737605536224507 1106 10-11 0.2427840638106673 1106 12-13 0.28261062992580577 1106 14-15 0.2511449773467973 1106 16-17 0.28439007593262744 1106 18-19 0.2744469255232005 1106 20-21 0.12662976741410858 1106 22-23 0.15849820951012106 1106 24-25 0.14477134264149072 1106 26-27 0.07401535004309068 1106 28-29 0.1188030925479282 1106 30-31 0.09913806358333943 1106 32-33 -0.0574200534387046 1106 34-35 0.17852315362316062 1106 36-37 -0.010334374575343475 1106 38-39 -0.04830183551012368 1106 40-41 0.116599460314454 1106 42-43 0.03953116054210426 1106 44-45 0.18097796569928448 1106 46-47 0.3455103663231682 1106 48-49 0.14519066693475224 1106 50-51 -0.011357207770409161 1106 52-53 -0.020562251045397772 1106 54-55 0.013327022631628438 1106 56-57 -0.03871573500990877 1106 58-59 0.025395890579339664 1106 60-61 8.392006914377248E-4 1106 62-63 0.1538987143730921 1106 64-65 0.33929494581793307 1106 66-67 0.07298015003443936 1106 68-69 0.009564472375963362 1106 70-71 0.05508077414666701 1106 72-73 0.09017449297738267 1106 74-75 0.05562289041455415 1106 76-77 0.08945760740812858 1106 78-79 0.12553603060526797 1106 80-81 0.08814184527466296 1106 82-83 0.0956796981615824 1106 84-85 -0.004181715243554152 1106 86-87 0.015901804110974638 1106 88-89 -0.019724548342672676 1106 90-91 0.004787650193655679 1106 92-93 0.08816440974915452 1106 94-95 -0.031921794014110105 1106 96-97 0.04681063280075648 1106 98-99 -0.04976170436845706 1106 100-101 -0.0247368553828764 1107 1 0.09975127149909468 1107 2 0.16158463236273946 1107 3 0.1243971094881644 1107 4 0.26763535979727493 1107 5 0.2662189367540293 1107 6 0.33753722838197575 1107 7 0.2440061183587403 1107 8 0.2876296036607684 1107 9 0.21794781067405467 1107 10-11 0.2284881676864643 1107 12-13 0.13615289153406707 1107 14-15 0.17826966963219348 1107 16-17 0.16426518023927628 1107 18-19 0.21043331801088527 1107 20-21 0.11586003644116971 1107 22-23 0.26220968412176404 1107 24-25 0.25398828604442514 1107 26-27 0.28063956263804357 1107 28-29 0.25387206706747634 1107 30-31 0.18160557837045843 1107 32-33 0.14736948338374134 1107 34-35 0.24610104525957155 1107 36-37 0.22669067984028857 1107 38-39 0.3359449383915134 1107 40-41 0.38113680329391997 1107 42-43 0.25076280377414406 1107 44-45 0.24547030266459302 1107 46-47 0.4653548152877818 1107 48-49 0.3800728905201396 1107 50-51 0.2125773429142086 1107 52-53 0.25298862737269445 1107 54-55 0.34144873426960487 1107 56-57 0.3867919440411427 1107 58-59 0.3746747532885806 1107 60-61 0.4170383226737542 1107 62-63 0.36979160326269067 1107 64-65 0.503081441130746 1107 66-67 0.4214216766512493 1107 68-69 0.386686201631953 1107 70-71 0.424786601384433 1107 72-73 0.46140123519862897 1107 74-75 0.5290566891303641 1107 76-77 0.4902552228901911 1107 78-79 0.5518837685325195 1107 80-81 0.5442651457370573 1107 82-83 0.3933741409598994 1107 84-85 0.45741101716606636 1107 86-87 0.35851961656242537 1107 88-89 0.353180389665944 1107 90-91 0.3952143424438077 1107 92-93 0.45984041129312914 1107 94-95 0.3553246867878528 1107 96-97 0.3964183116916544 1107 98-99 0.31768485299178195 1107 100-101 0.4482752321754404 1108 1 0.11008163829015416 1108 2 0.1256467388457665 1108 3 0.15439514666469734 1108 4 0.09642815763930201 1108 5 0.11959811511075458 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52-53 -1.3799642009327968 2316 54-55 -1.3053929508562732 2316 56-57 -1.2580675228012197 2316 58-59 -1.5447883276203953 2316 60-61 -0.9531971695181696 2316 62-63 -0.8273397793867758 2316 64-65 -1.2325149675981137 2316 66-67 -1.5544289916901661 2316 68-69 -1.7290183784708901 2316 70-71 -1.230417301466609 2316 72-73 -1.0428997807105418 2316 74-75 -1.28772471397415 2316 76-77 -1.08202768343412 2316 78-79 -1.3677495436495946 2316 80-81 -1.1272832682679166 2316 82-83 -1.2087601100130527 2316 84-85 -1.8538073286855266 2316 86-87 -1.2855190248870585 2316 88-89 -0.9968050325758284 2316 90-91 -1.1985669265327275 2316 92-93 -1.4072761333184758 2316 94-95 -1.2639083615042992 2316 96-97 -1.025686292591999 2316 98-99 -0.7612865051242288 2316 100-101 -1.2464818335243848 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 199.0 8 1013.0 9 1099.0 10 704.0 11 764.0 12 969.0 13 1083.0 14 1438.0 15 1668.0 16 2145.0 17 2624.0 18 3240.0 19 4252.0 20 4867.0 21 5722.0 22 6106.0 23 6864.0 24 7708.0 25 9012.0 26 10668.0 27 12936.0 28 15651.0 29 18984.0 30 23598.0 31 29326.0 32 37671.0 33 49086.0 34 69769.0 35 103428.0 36 171696.0 37 333725.0 38 650420.0 39 332996.0 40 214.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.81640045003785 21.13463074728869 14.312071554345415 22.73689724832804 2 13.4013258328498 20.447992161782317 44.60221619755568 21.54846580781221 3 17.907425994238565 26.604742104096875 31.06602187259669 24.421810029067874 4 11.452750117216572 17.04013422405759 41.68320389529313 29.823911763432708 5 11.732869123210437 39.241700870341816 37.25046271469022 11.774967291757523 6 28.72161320176754 40.27847804267633 18.70975142406507 12.290157331491065 7 25.58200036181959 33.07886050812191 24.20531332585712 17.133825804201383 8 22.923508364358298 40.39286181643196 20.932401637263116 15.751228181946624 9 24.204041818531596 17.649420232657683 22.133030811267627 36.013507137543094 10-11 23.02589213600085 28.20103439231928 30.331998325200054 18.441075146479818 12-13 23.142589910503975 25.152653130171203 31.019110837225256 20.685646122099566 14-15 20.271975760881155 27.645489851746046 27.773886061854906 24.308648325517893 16-17 20.365941311888665 31.286159230825323 28.304826091938455 20.043073365347556 18-19 20.343884887217595 30.47898326516769 29.586318318671623 19.59081352894309 20-21 20.672373772499995 29.533427055386817 29.829736534870356 19.964462637242832 22-23 20.654771321282567 29.17053487423295 29.74155516514991 20.433138639334572 24-25 20.463971549420084 29.44483068699366 29.44509088032772 20.646106883258536 26-27 20.440762304022382 29.669377534283075 29.401352380873085 20.48850778082146 28-29 20.519548845574214 29.366798706110586 29.687851261000976 20.42580118731422 30-31 20.59125812843976 29.522446358542254 29.527728283223563 20.358567229794428 32-33 20.28735231426359 29.671172868288053 29.620539245480966 20.420935571967394 34-35 20.47903674346186 29.527676244556755 29.531553125234172 20.461733886747208 36-37 20.66994059265797 29.351889628069237 29.404188488214285 20.57398129105851 38-39 20.616028533841785 29.281689466541216 29.518751613198674 20.583530386418325 40-41 20.568673347043788 29.241125325762052 29.748033979167882 20.442167348026278 42-43 20.509765576213752 29.18934685228512 29.6500711888962 20.65081638260493 44-45 20.420033345657522 29.36166176956061 29.389749454205216 20.82855543057665 46-47 20.67734335646176 29.326332926248032 29.25974267063949 20.736581046650723 48-49 20.65160523682129 28.996998148159243 29.495258157435373 20.8561384575841 50-51 20.428441170053276 29.307389198987483 29.503342347282302 20.760827283676942 52-53 20.354142683536207 29.25658523273651 29.74062675682805 20.64864532689924 54-55 20.300126300438798 29.338992981862216 29.542063254384708 20.81881746331428 56-57 20.56261984567537 29.38729928250848 29.352939506214327 20.697141365601826 58-59 20.46775964595722 29.134926720312038 29.438687383058788 20.958626250671955 60-61 20.498791221332873 29.12833482465544 29.455869807319797 20.917004146691898 62-63 20.4681162935664 29.158835603754735 29.56625952964067 20.806788573038194 64-65 20.350049946093655 29.187147517884977 29.475125698971745 20.987676837049634 66-67 20.50427894752608 29.118462574349234 29.291000991106092 21.086257487018596 68-69 20.609396722095497 28.80629396841919 29.41598764462512 21.168321664860194 70-71 20.556235578450625 28.858959200362527 29.358602405371197 21.226202815815654 72-73 20.418738965820733 28.960378508152925 29.328340719832095 21.292541806194244 74-75 20.490420984719226 28.716252484519632 29.37129536886583 21.422031161895312 76-77 20.46558139656166 28.691756692977034 29.60070300052934 21.241958909931967 78-79 20.620587209137202 28.534916303377226 29.330596383547135 21.51390010393843 80-81 20.687635534728532 28.841295424886642 29.08099022808768 21.390078812297144 82-83 20.481882213225653 28.821936930543384 29.251680128967234 21.44450072726373 84-85 20.690972757619072 28.53547718966315 29.35265485066074 21.420895202057036 86-87 20.5750861449631 28.455556802555197 29.489009925972486 21.480347126509216 88-89 20.76105236317588 28.227962535483385 29.357674868399908 21.65331023294082 90-91 21.021952269158348 28.350099460962223 29.026684089267906 21.60126418061152 92-93 20.975388795830813 28.09674240554446 29.236096432781473 21.691772365843253 94-95 20.91159995716641 27.92583535277398 29.44608920695061 21.716475483109 96-97 20.86651629021769 27.930433052399707 29.336403367244863 21.866647290137735 98-99 21.053780084119918 27.770639898839445 29.188913586470814 21.98666643056982 100-101 21.264932394309724 27.96928810613199 28.929392794645388 21.836386704912897 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 21.0 1 14.5 2 18.0 3 34.5 4 29.0 5 20.0 6 39.5 7 80.0 8 146.5 9 191.0 10 194.0 11 189.5 12 199.5 13 229.5 14 266.5 15 318.0 16 347.5 17 363.0 18 432.0 19 498.5 20 567.5 21 632.5 22 724.5 23 854.5 24 1007.0 25 1191.5 26 1459.0 27 1966.0 28 3006.0 29 5537.0 30 10562.5 31 18852.0 32 32010.0 33 49601.0 34 69663.5 35 91652.5 36 115257.5 37 133831.0 38 142226.5 39 141666.0 40 133056.5 41 120599.5 42 109311.0 43 95886.0 44 80316.0 45 65175.5 46 52150.0 47 43812.5 48 38280.5 49 34385.5 50 31721.5 51 30071.0 52 28725.0 53 28075.5 54 27413.0 55 25846.5 56 23794.0 57 21543.0 58 19757.0 59 17599.0 60 14755.0 61 12202.0 62 9798.0 63 7595.0 64 5637.0 65 4150.5 66 3131.0 67 2429.0 68 1925.0 69 1500.0 70 1175.5 71 892.5 72 668.5 73 549.0 74 449.5 75 369.0 76 285.0 77 194.5 78 139.0 79 97.0 80 71.5 81 53.0 82 38.0 83 23.0 84 16.5 85 18.5 86 15.0 87 11.5 88 9.5 89 8.5 90 8.0 91 8.5 92 6.0 93 3.5 94 2.5 95 0.5 96 0.0 97 0.5 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46418490795400613 2 0.3604718449997086 3 0.19373995653730547 4 0.22152860461437268 5 0.24458173401164002 6 0.19373995653730547 7 0.1855178471811695 8 0.18572600184841345 9 0.18478930584581568 10-11 0.19811120454942843 12-13 0.18598619518246837 14-15 0.19548325187547355 16-17 0.08680049624072671 18-19 0.03718162743645038 20-21 4.4232866789339155E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.13829275705019858 46-47 0.2055527339033996 48-49 0.08672243824051022 50-51 0.06718191885298452 52-53 0.07605451154425784 54-55 0.08445875623423228 56-57 0.11721709699174875 58-59 0.097832693604656 60-61 0.14409506839962366 62-63 0.23953398333097425 64-65 0.13420772170553608 66-67 0.108006252966204 68-69 0.11237750097832692 70-71 0.09252474958993531 72-73 0.07691314954663914 74-75 0.1439389523991907 76-77 0.21611658326602998 78-79 0.14172730905972372 80-81 0.08880398491294972 82-83 0.09265484625696277 84-85 0.10366102428748657 86-87 0.07719936221409957 88-89 0.0994719116092021 90-91 0.11979301099889263 92-93 0.16613344379407674 94-95 0.13496228237429542 96-97 0.09364358092637153 98-99 0.07972323755443245 100-101 0.06684366751871311 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1921648.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.39060646989916 #Duplication Level Percentage of deduplicated Percentage of total 1 66.74518157711344 30.963494523058333 2 15.369349849872126 14.259869211672429 3 6.518166740246673 9.071451244559064 4 3.498451564754954 6.491811591782001 5 2.0814736503181033 4.828041249468582 6 1.3531433844110963 3.7663885346137587 7 0.9343408033142866 3.0341245560725763 8 0.6665553989128421 2.473752736108185 9 0.4922711781391108 2.0553082651372603 >10 2.2260420300983235 17.24508622764392 >50 0.07914160891062778 2.462958338236106 >100 0.03419020561000492 2.7764393488940686 >500 0.0014664071919970015 0.4457372358263211 >1k 2.2560110646107693E-4 0.12553693692737067 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.601933340549362E-4 0.0 10-11 0.0 0.0 0.0 5.46406001515366E-4 0.0 12-13 0.0 0.0 0.0 7.805800021648086E-4 0.0 14-15 0.0 0.0 0.0 0.0015091213375186298 0.0 16-17 0.0 0.0 0.0 0.0029662040082262726 0.0 18-19 0.0 0.0 0.0 0.004735518679799839 0.0 20-21 0.0 1.0407733362197447E-4 0.0 0.008065993355703021 0.0 22-23 0.0 1.0407733362197447E-4 0.0 0.015013155374969816 0.0 24-25 0.0 1.300966670274681E-4 0.0 0.023547496731971727 0.0 26-27 0.0 1.561160004329617E-4 0.0 0.029115634080747358 0.0 28-29 0.0 1.561160004329617E-4 0.0 0.03590668009958119 0.0 30-31 0.0 1.8213533383845532E-4 0.0 0.04982702347152028 0.0 32-33 0.0 2.0815466724394895E-4 0.0 0.08778923091013546 0.0 34-35 0.0 2.0815466724394895E-4 0.0 0.14443331973389506 0.0 36-37 0.0 2.0815466724394895E-4 0.0 0.23024508130521304 0.0 38-39 0.0 2.0815466724394895E-4 0.0 0.36596192434826774 0.0 40-41 0.0 2.0815466724394895E-4 0.0 0.5210371514450096 0.0 42-43 0.0 2.0815466724394895E-4 0.0 0.6740568512027176 0.0 44-45 0.0 2.0815466724394895E-4 0.0 0.8533560776999742 0.0 46-47 0.0 2.0815466724394895E-4 0.0 1.082222134334696 0.0 48-49 0.0 2.0815466724394895E-4 0.0 1.3085643156290852 0.0 50-51 0.0 2.0815466724394895E-4 0.0 1.5424521036110672 0.0 52-53 0.0 2.0815466724394895E-4 0.0 1.771318160245789 0.0 54-55 0.0 2.0815466724394895E-4 0.0 2.016316203591917 0.0 56-57 0.0 2.0815466724394895E-4 0.0 2.31665736909153 0.0 58-59 0.0 2.0815466724394895E-4 0.0 2.634925855307528 0.0 60-61 0.0 2.0815466724394895E-4 0.0 2.9642525582208603 0.0 62-63 0.0 2.0815466724394895E-4 0.0 3.286241809113844 0.0 64-65 0.0 2.0815466724394895E-4 0.0 3.6202519920401657 0.0 66-67 0.0 2.0815466724394895E-4 0.0 4.004505507772495 0.0 68-69 0.0 2.0815466724394895E-4 0.0 4.438898278977211 0.0 70-71 0.0 2.0815466724394895E-4 0.0 4.9028490129305675 0.0 72-73 0.0 2.0815466724394895E-4 0.0 5.358057250859678 0.0 74-75 0.0 2.0815466724394895E-4 0.0 5.808373854108557 0.0 76-77 0.0 2.0815466724394895E-4 0.0 6.313877463510487 0.0 78-79 0.0 2.0815466724394895E-4 0.0 6.876779722404936 0.0 80-81 0.0 2.0815466724394895E-4 0.0 7.484799505424511 0.0 82-83 0.0 2.0815466724394895E-4 0.0 8.116548920509896 0.0 84-85 0.0 2.0815466724394895E-4 0.0 8.722695311524275 0.0 86-87 0.0 2.0815466724394895E-4 0.0 9.350854058599701 0.0 88-89 0.0 2.0815466724394895E-4 0.0 10.076325112611675 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1900 0.0 33.59543 1 GTATCAA 3110 0.0 26.957605 1 TCAACGC 2735 0.0 26.940619 4 CAACGCA 2900 0.0 25.571712 5 ATCAACG 2930 0.0 25.470135 3 AACGCAG 3075 0.0 24.416044 6 GTACATG 4600 0.0 24.128323 1 ACATGGG 4365 0.0 22.759424 3 CGCAGAG 3460 0.0 21.150433 8 ACGCAGA 3585 0.0 20.942085 7 GTATAGG 1090 0.0 20.539997 1 TACATGG 4910 0.0 20.354404 2 TATCAAC 3705 0.0 20.295017 2 CATGGGG 2290 0.0 19.92824 4 ACGACTA 120 0.004743137 19.807146 5 GACCGTC 120 0.0047545996 19.798887 7 GTATAGC 675 0.0 19.759832 1 GTACGAC 130 0.007534288 18.282091 3 GAGTACA 3670 0.0 18.041712 1 GTCGCCG 675 0.0 17.943972 94-95 >>END_MODULE