FastQCFastQC Report
Fri 10 Feb 2017
SRR2031616_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031616_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921648
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22600.027.7525711
GTATCAA32800.026.0757221
TCAACGC31400.025.7163934
GTACATG48250.025.7028261
ATCAACG32200.025.0774773
ACATGGG44200.024.7170313
CAACGCA33300.024.2478285
AACGCAG33850.023.713536
CATGGGG24250.022.9173764
GTACACG2704.0017767E-1122.877961
TACATGG52500.022.2569982
GTATAGG10000.021.8572521
ACGCAGA38150.020.9162047
CGCAGAG37550.020.8709478
TATCAAC40150.020.5851692
GCACCGT1404.855287E-420.355956
CGTACAC1656.325867E-520.1513823
AAGACCG3153.2196112E-1019.6020265
GACCGCA2302.0604766E-618.5858677
CCTACAC10450.018.18173
Produced by FastQC (version 0.11.5)