##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031615_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 942958 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10677039698481 33.0 31.0 34.0 30.0 34.0 2 32.32428803827954 34.0 31.0 34.0 31.0 34.0 3 32.45574882444393 34.0 31.0 34.0 31.0 34.0 4 35.5439086364398 37.0 35.0 37.0 33.0 37.0 5 35.37749931598226 37.0 35.0 37.0 33.0 37.0 6 35.627023685042175 37.0 36.0 37.0 33.0 37.0 7 35.728994292428716 37.0 36.0 37.0 35.0 37.0 8 35.779831127155184 37.0 36.0 37.0 35.0 37.0 9 37.67644264113566 39.0 38.0 39.0 35.0 39.0 10-11 37.626229906316084 39.0 38.0 39.0 35.0 39.0 12-13 37.70261559899805 39.0 38.0 39.0 35.0 39.0 14-15 39.061843687629775 41.0 39.0 41.0 36.0 41.0 16-17 39.078316319496736 41.0 39.0 41.0 36.0 41.0 18-19 39.09092080453212 41.0 39.0 41.0 36.0 41.0 20-21 39.1101104184916 41.0 39.0 41.0 36.0 41.0 22-23 39.02047917298543 41.0 39.0 41.0 35.5 41.0 24-25 38.94522025371226 41.0 39.0 41.0 35.0 41.0 26-27 38.823885581330245 41.0 39.0 41.0 35.0 41.0 28-29 38.779347542520455 40.0 39.0 41.0 35.0 41.0 30-31 38.68106320748114 40.0 38.0 41.0 35.0 41.0 32-33 38.51455048899315 40.0 38.0 41.0 34.0 41.0 34-35 38.38228637966908 40.0 38.0 41.0 34.0 41.0 36-37 38.24043806829148 40.0 38.0 41.0 33.5 41.0 38-39 38.061250342008876 40.0 38.0 41.0 33.0 41.0 40-41 38.06657878717822 40.0 38.0 41.0 33.0 41.0 42-43 37.92808216272623 40.0 38.0 41.0 33.0 41.0 44-45 37.693305004040475 40.0 38.0 41.0 33.0 41.0 46-47 37.46452652185994 40.0 38.0 41.0 32.0 41.0 48-49 37.53698998258671 40.0 38.0 41.0 32.5 41.0 50-51 37.24733604253848 39.5 37.5 40.5 31.5 41.0 52-53 37.50302452495233 40.0 38.0 40.5 32.5 41.0 54-55 37.86587260514254 40.0 38.0 41.0 33.0 41.0 56-57 37.751840484942065 40.0 38.0 41.0 32.5 41.0 58-59 37.627231541595705 40.0 38.0 41.0 32.0 41.0 60-61 37.31284320192416 40.0 37.5 41.0 31.0 41.0 62-63 37.08646673552799 40.0 37.0 41.0 31.0 41.0 64-65 36.90180209510922 39.5 37.0 41.0 31.0 41.0 66-67 36.65372052625886 39.0 36.0 41.0 30.5 41.0 68-69 36.34931619435861 39.0 36.0 41.0 30.0 41.0 70-71 35.928668084898796 38.0 35.0 40.0 29.5 41.0 72-73 35.520001421060115 37.0 35.0 39.5 29.0 41.0 74-75 35.1002584420515 37.0 35.0 39.0 29.0 41.0 76-77 34.69159548993699 36.0 34.5 39.0 29.0 41.0 78-79 34.27579489224334 36.0 34.0 38.0 28.0 39.5 80-81 33.8404271452175 35.5 34.0 37.0 27.0 39.0 82-83 33.429232266972654 35.0 34.0 37.0 26.5 39.0 84-85 33.07335745600547 35.0 34.0 36.0 26.0 38.0 86-87 32.92234012543507 35.0 34.0 36.0 26.0 37.0 88-89 32.61739653303753 35.0 34.0 36.0 26.0 37.0 90-91 32.37800517096201 35.0 33.0 35.0 25.0 36.5 92-93 32.16405979905785 35.0 33.0 35.0 25.0 36.0 94-95 31.95701664337118 35.0 33.0 35.0 25.0 36.0 96-97 31.814346980459362 35.0 33.0 35.0 24.0 36.0 98-99 31.810368012149006 35.0 33.0 35.0 24.0 35.5 100-101 31.359256721932475 34.5 32.0 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06760785863819052 1101 2 0.11331145044903224 1101 3 0.15490395288092174 1101 4 0.13357203628088854 1101 5 0.14480381766238537 1101 6 0.1560312200005427 1101 7 0.08071132727481256 1101 8 0.2266997897573617 1101 9 0.10378938307006536 1101 10-11 0.20165662980535615 1101 12-13 0.18653943383412752 1101 14-15 0.3035090448311877 1101 16-17 0.26500301731434206 1101 18-19 0.26612978690004496 1101 20-21 0.031228908979244352 1101 22-23 0.24553589092344907 1101 24-25 0.2923116804530892 1101 26-27 0.19709621793914778 1101 28-29 0.2096651808325447 1101 30-31 0.18426289267789286 1101 32-33 0.2294619115731038 1101 34-35 0.27523144556298007 1101 36-37 0.19478355816978876 1101 38-39 0.22910453465051717 1101 40-41 0.05959750486383797 1101 42-43 0.0022675836541310446 1101 44-45 0.013759891740768637 1101 46-47 0.2569996848143248 1101 48-49 0.07324149543809 1101 50-51 0.06243021285063577 1101 52-53 -0.04225121385836417 1101 54-55 -0.03006159558000121 1101 56-57 0.11039316899246643 1101 58-59 0.042435991670451756 1101 60-61 -0.3895528059373916 1101 62-63 -0.3186437253091654 1101 64-65 -0.1373845462000034 1101 66-67 -0.13718673618698318 1101 68-69 -0.17800123171694082 1101 70-71 -0.1590005750311363 1101 72-73 -0.11369611566901483 1101 74-75 -0.14024664451945767 1101 76-77 -0.19355618099923788 1101 78-79 -0.24662522418767452 1101 80-81 -0.26806058609767547 1101 82-83 -0.1722444857429153 1101 84-85 -0.3008542961752738 1101 86-87 -0.26864121638458016 1101 88-89 -0.39004208886468916 1101 90-91 -0.3313025523928914 1101 92-93 -0.18020840596178545 1101 94-95 -0.16082695459320462 1101 96-97 -0.08752416753134895 1101 98-99 -0.3636963395984836 1101 100-101 -0.4837261006749891 1102 1 -1.5386692663810209E-4 1102 2 0.08536546085893804 1102 3 0.23587631327713154 1102 4 0.07499828020723953 1102 5 0.024999760393470183 1102 6 0.1582588250007575 1102 7 0.053024864480860856 1102 8 0.18020484332080855 1102 9 0.36572401115375186 1102 10-11 0.23732534385007398 1102 12-13 0.17769209326287694 1102 14-15 0.08760798221313593 1102 16-17 0.29212356751115465 1102 18-19 0.4198795886504101 1102 20-21 0.4117312375113329 1102 22-23 0.19365035665870067 1102 24-25 0.3040985224445265 1102 26-27 0.27984922051999206 1102 28-29 0.1893680226509673 1102 30-31 0.30602241226495863 1102 32-33 0.33406923311238046 1102 34-35 0.059571887046899974 1102 36-37 0.12985500012950268 1102 38-39 0.12096656732205702 1102 40-41 0.31924325078710325 1102 42-43 0.22554713726804465 1102 44-45 -0.10391928988472188 1102 46-47 -0.46134165059522303 1102 48-49 0.019344628503247918 1102 50-51 -0.11904389935144621 1102 52-53 0.009672168064156494 1102 54-55 0.05347539419752678 1102 56-57 0.011879320354253764 1102 58-59 0.06299447891759513 1102 60-61 0.07705654856179223 1102 62-63 -0.3867659047575174 1102 64-65 -0.01979908258422114 1102 66-67 0.10031092086277482 1102 68-69 0.2972301796762835 1102 70-71 0.3301443562663593 1102 72-73 0.1512969636164243 1102 74-75 0.18558384122818694 1102 76-77 0.14907686252917074 1102 78-79 0.34936004045756164 1102 80-81 0.4739283263408609 1102 82-83 0.16683628476331336 1102 84-85 0.36340453854940336 1102 86-87 0.06208062856258323 1102 88-89 0.054455222149343285 1102 90-91 0.31719904081327144 1102 92-93 0.20261524558024036 1102 94-95 0.3454755815603221 1102 96-97 0.44378305452266886 1102 98-99 0.33661329028747033 1102 100-101 0.3193543382136603 1103 1 0.3383645219592566 1103 2 0.2187118073130847 1103 3 -0.08903214614885258 1103 4 0.10066666101602806 1103 5 0.1873765844740305 1103 6 0.14248153783259454 1103 7 0.297582911974267 1103 8 0.05261007190300404 1103 9 0.19064256696657367 1103 10-11 0.17368950033061026 1103 12-13 0.08907442323972248 1103 14-15 0.3286289288503639 1103 16-17 0.2804555097867478 1103 18-19 0.387411291305078 1103 20-21 0.06737881976322768 1103 22-23 0.26255314864621226 1103 24-25 0.21561378818142884 1103 26-27 0.09946044231742235 1103 28-29 0.22016200251204054 1103 30-31 0.2551652290222677 1103 32-33 0.06885566087610329 1103 34-35 0.13563974822831426 1103 36-37 -0.03727217395912419 1103 38-39 0.04487346517354496 1103 40-41 0.008733921725664118 1103 42-43 0.08197651640756476 1103 44-45 0.03875780074044144 1103 46-47 0.2914314903234114 1103 48-49 0.12399704355897967 1103 50-51 0.26508996527619644 1103 52-53 0.042845529757116196 1103 54-55 0.11552778771429928 1103 56-57 0.10803730861552197 1103 58-59 0.12747000527590302 1103 60-61 0.27483467036460496 1103 62-63 0.43228328483916556 1103 64-65 0.3144771410978606 1103 66-67 0.06376536596535942 1103 68-69 0.08214011990567371 1103 70-71 0.15291742987574963 1103 72-73 0.44271907335741645 1103 74-75 0.1326602641456418 1103 76-77 -0.3632861631560331 1103 78-79 -0.02339351347060159 1103 80-81 0.04999450479113676 1103 82-83 -0.2142770774681253 1103 84-85 0.09393702199133713 1103 86-87 0.1591321470591538 1103 88-89 0.06385832375937639 1103 90-91 0.028555120184336147 1103 92-93 -0.2384218552759343 1103 94-95 -0.14608888723510915 1103 96-97 -0.16537907998813495 1103 98-99 -0.05034251446187099 1103 100-101 -0.16998892547703903 1104 1 0.20030675648018814 1104 2 0.1931583655974194 1104 3 0.1378357196415294 1104 4 0.11115178726991815 1104 5 -0.18758017789193815 1104 6 0.09907095325978332 1104 7 0.1684969555577993 1104 8 0.2745272285961917 1104 9 0.23385858218622246 1104 10-11 0.37899259614520986 1104 12-13 0.09209024821377909 1104 14-15 0.2946441484975537 1104 16-17 0.28043389096972504 1104 18-19 0.23269622731337591 1104 20-21 0.3497962358822164 1104 22-23 0.337408302572193 1104 24-25 0.2234830375761021 1104 26-27 0.29731585566919705 1104 28-29 0.16183443419657806 1104 30-31 0.2072471211011333 1104 32-33 0.09403077329394449 1104 34-35 0.26521212802768446 1104 36-37 0.35825489279977774 1104 38-39 0.5221737073042974 1104 40-41 0.4222478442438202 1104 42-43 0.20529024868012868 1104 44-45 0.27754844411643376 1104 46-47 0.3584498230802424 1104 48-49 0.34148234276340617 1104 50-51 0.3163697992436809 1104 52-53 0.3530106975191316 1104 54-55 0.17273117754480438 1104 56-57 0.3481791280551576 1104 58-59 0.26993800942670987 1104 60-61 0.45948098651818015 1104 62-63 0.6200859482774206 1104 64-65 0.6572326755150257 1104 66-67 0.43662510308053726 1104 68-69 0.32373040008554455 1104 70-71 0.3232845173887213 1104 72-73 0.3738847599710411 1104 74-75 0.3128206757679166 1104 76-77 0.3754180049518894 1104 78-79 0.491880180752851 1104 80-81 0.39403635882088395 1104 82-83 0.4512041573986991 1104 84-85 0.3276040057339884 1104 86-87 0.36404610416742855 1104 88-89 0.5085573898264855 1104 90-91 0.397512660827708 1104 92-93 0.2875207251460665 1104 94-95 0.3007612511253157 1104 96-97 0.5521062192162987 1104 98-99 0.38450918045338156 1104 100-101 0.23829324332268342 1105 1 0.18607860759351524 1105 2 0.20845770567242994 1105 3 0.30845184744858045 1105 4 0.2322943120764478 1105 5 -0.1491330193675111 1105 6 0.13537869669613656 1105 7 0.19831820609191197 1105 8 0.15315028798944041 1105 9 0.28600571222467863 1105 10-11 0.29027363398407147 1105 12-13 0.2898422303625665 1105 14-15 0.37883651188357703 1105 16-17 0.268388182855972 1105 18-19 0.4316915033899136 1105 20-21 0.1508246981145689 1105 22-23 0.26647971927446434 1105 24-25 0.38674474056237784 1105 26-27 0.3210615827736092 1105 28-29 0.4076119825040365 1105 30-31 0.4193058852847784 1105 32-33 0.4207589221806174 1105 34-35 0.33078030445079776 1105 36-37 0.4146585983497886 1105 38-39 0.5342817284788666 1105 40-41 0.5483872830702055 1105 42-43 0.49786787295018087 1105 44-45 0.5346936750901818 1105 46-47 0.6253900737567832 1105 48-49 0.8802870595653829 1105 50-51 0.5811038708384189 1105 52-53 0.3818186189928099 1105 54-55 0.43284221348460505 1105 56-57 0.5240162001721487 1105 58-59 0.4658428544155555 1105 60-61 0.765893593934031 1105 62-63 0.8594541531864976 1105 64-65 0.8726504104055905 1105 66-67 0.5844361342726785 1105 68-69 0.546825994255407 1105 70-71 0.48272435264485836 1105 72-73 0.6007948036495279 1105 74-75 0.5840783313725311 1105 76-77 0.5112011317619363 1105 78-79 0.5143840975738172 1105 80-81 0.6989415836226698 1105 82-83 0.7975401494226304 1105 84-85 0.6626019879777232 1105 86-87 0.64490145478225 1105 88-89 0.6776913569726659 1105 90-91 0.8264742878514042 1105 92-93 0.8117857277572753 1105 94-95 0.6699750383351102 1105 96-97 0.746110515850205 1105 98-99 0.480907485538129 1105 100-101 0.3432788816458121 1106 1 0.12393838453181161 1106 2 0.009411114558922407 1106 3 0.11188996581519461 1106 4 0.030279561221981055 1106 5 0.05448948467998349 1106 6 -0.058136323991035965 1106 7 -0.03899965683307016 1106 8 0.11237182063919704 1106 9 0.12612983526839372 1106 10-11 0.1240791745489247 1106 12-13 0.15117070670723365 1106 14-15 0.09931793839913894 1106 16-17 0.18962514094195626 1106 18-19 0.2787413324958692 1106 20-21 0.1076627085480979 1106 22-23 0.08458436939138636 1106 24-25 0.08332348673975076 1106 26-27 0.10966452395007309 1106 28-29 0.2097121803813593 1106 30-31 0.16953636738586653 1106 32-33 0.2506743746001163 1106 34-35 0.26439021630545056 1106 36-37 0.033120776799151486 1106 38-39 0.21615459230329748 1106 40-41 0.22846688371780743 1106 42-43 0.022928585308520155 1106 44-45 0.4550480553744052 1106 46-47 0.5719969275074632 1106 48-49 0.4181367177772941 1106 50-51 0.1957209332597145 1106 52-53 0.15182558967098458 1106 54-55 0.2249642228387998 1106 56-57 0.14654835523600696 1106 58-59 0.09901091521785332 1106 60-61 0.11722139569693724 1106 62-63 0.1330532717329973 1106 64-65 0.3692831231237008 1106 66-67 0.044855049545212466 1106 68-69 0.05261615435615141 1106 70-71 0.12778753846964008 1106 72-73 0.07901143433850422 1106 74-75 0.03931983164236641 1106 76-77 0.04322127927048314 1106 78-79 -0.05705411073703459 1106 80-81 0.11835717095852516 1106 82-83 0.19443032751221523 1106 84-85 0.13947507666274817 1106 86-87 0.004391695832794085 1106 88-89 -0.025801852237620437 1106 90-91 -0.17869188412197445 1106 92-93 -0.10764110260789295 1106 94-95 -0.3738727760199829 1106 96-97 -0.14974530354177418 1106 98-99 -0.2163402208838896 1106 100-101 -0.17153943100035107 1107 1 0.20434046540926687 1107 2 0.16540260653780336 1107 3 0.015001031367958717 1107 4 0.21921785244231984 1107 5 0.13379090439193675 1107 6 0.11513003857367465 1107 7 0.1356415711921457 1107 8 0.1632431240065273 1107 9 0.24736785484709145 1107 10-11 0.27935376324248296 1107 12-13 0.2097184490064521 1107 14-15 0.2974523241302691 1107 16-17 0.2097323881358477 1107 18-19 0.18937443689851108 1107 20-21 0.08000779593470497 1107 22-23 0.2112142192196913 1107 24-25 0.4035627107672255 1107 26-27 0.29793268037465737 1107 28-29 0.23795073811801615 1107 30-31 0.28733017600664823 1107 32-33 0.3883210867112368 1107 34-35 0.3263014458682605 1107 36-37 0.42750022941562804 1107 38-39 0.34840498951513155 1107 40-41 0.29044282036892355 1107 42-43 0.25888906913814935 1107 44-45 0.388352345262156 1107 46-47 0.6127175466453849 1107 48-49 0.6507157757445938 1107 50-51 0.36602486437549686 1107 52-53 0.4159714561053107 1107 54-55 0.48146952753871375 1107 56-57 0.46579583101890165 1107 58-59 0.5430412058231582 1107 60-61 0.6534189252956324 1107 62-63 0.6736739472493767 1107 64-65 0.6601510950369871 1107 66-67 0.4084851812657817 1107 68-69 0.5174539381704122 1107 70-71 0.6166252454041938 1107 72-73 0.5390307823985694 1107 74-75 0.6938384365319124 1107 76-77 0.5776377622800624 1107 78-79 0.5496946463743413 1107 80-81 0.7349990652745433 1107 82-83 0.7189581579668314 1107 84-85 0.7046178166555706 1107 86-87 0.5747411141096705 1107 88-89 0.467281536619943 1107 90-91 0.5590483649682838 1107 92-93 0.5650384115123472 1107 94-95 0.528502806984477 1107 96-97 0.3428639937776836 1107 98-99 0.21421754140405014 1107 100-101 0.43990091034555334 1108 1 0.056551471359405525 1108 2 0.20004674909890952 1108 3 0.020982684078248326 1108 4 0.004012869347512549 1108 5 0.09356986438903192 1108 6 0.02941765718271938 1108 7 -0.03498216394763887 1108 8 0.011857382540924277 1108 9 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Count 5 2.0 6 5.0 7 72.0 8 319.0 9 403.0 10 404.0 11 510.0 12 594.0 13 780.0 14 866.0 15 1108.0 16 1664.0 17 2559.0 18 3240.0 19 4078.0 20 5129.0 21 5846.0 22 5761.0 23 5061.0 24 4926.0 25 5154.0 26 5972.0 27 6777.0 28 8168.0 29 9738.0 30 11700.0 31 14703.0 32 18464.0 33 24464.0 34 34663.0 35 52167.0 36 89699.0 37 176524.0 38 327645.0 39 113738.0 40 55.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.28915119524524 24.935551285617304 15.165882838151933 20.609414680985523 2 12.640222780972495 25.703695345316895 40.552885997202466 21.103195876508146 3 17.800072900161847 30.545486619207175 33.09327288783471 18.561167592796274 4 9.29287732449492 19.34813158124119 47.49963326820744 23.859357826056453 5 8.45302839401817 43.93879948751482 38.30475856312765 9.303413555339361 6 23.032788453159505 45.79852115085834 19.094220841188438 12.074469554793719 7 22.231750353054437 34.966787911245994 28.434351001463227 14.367110734236347 8 21.333771130068136 35.74227798817524 23.78075734584667 19.14319353590996 9 23.46192345374135 18.871868219212434 26.787471389194096 30.878736937852125 10-11 20.939252383626982 27.811930595711466 33.425669044227085 17.823147976434466 12-13 23.53060565230143 27.598962030374004 30.71664321813089 18.15378909919368 14-15 20.717484166579432 30.03479925204723 30.32763583478087 18.920080746592475 16-17 17.670521160795936 32.699226854326234 30.860491444020845 18.769760540856982 18-19 16.929117568738867 34.52071899841819 31.282085829560923 17.268077603282027 20-21 19.04574550668324 31.572841150594517 29.989681384921457 19.391731957800786 22-23 18.845006882597104 30.775654907217504 30.47007395875532 19.909264251430077 24-25 21.04351413318515 30.94326576581354 29.131944370799122 18.881275730202194 26-27 19.38050263108219 29.679794858307577 33.352386850739904 17.587315659870324 28-29 18.22530802008149 31.72463672825301 31.940287902536486 18.109767349129015 30-31 19.42907319307965 30.638692285340387 29.945766407411572 19.986468114168392 32-33 18.746434093565142 31.936576178366376 31.007372544694462 18.309617183374023 34-35 20.192999051919596 30.363176302656107 31.106422555405437 18.337402090018855 36-37 20.134671957817847 28.915603876312623 31.91138947864062 19.03833468722891 38-39 18.51259547084812 32.23271874251027 31.706237181295453 17.548448605346156 40-41 17.710810025473034 31.707509772439497 32.5198471193839 18.061833082703576 42-43 17.74039776957192 31.62224616578893 28.669569588465233 21.967786476173913 44-45 19.02661915963531 30.512258194697406 29.867939656866128 20.593182988801157 46-47 20.30966707972615 30.759363449124606 31.171873837605986 17.759095633543264 48-49 18.744842175204973 30.769161368867465 31.055531762117273 19.430464693810293 50-51 16.845039663695584 30.562030136025438 32.20115868608669 20.391771514192286 52-53 18.55493353851052 31.775861611525297 29.847231434559973 19.821973415404205 54-55 18.1287406242272 29.78600896423117 30.540475424782077 21.544774986759556 56-57 20.117375378244944 29.26437330785157 29.432818389340127 21.18543292456336 58-59 21.266352322762263 28.93273097058811 29.299636544283423 20.501280162366196 60-61 20.33474682919214 31.128609007505016 30.503208773656944 18.033435389645906 62-63 18.177493342405025 31.17051416300344 31.829257272857742 18.82273522173379 64-65 17.70903684825316 31.997185940320698 30.117765742805563 20.17601146862058 66-67 19.393070794252377 29.86857121624706 28.911784533231415 21.82657345626914 68-69 18.08095065448799 30.67863018017994 29.875536072880966 21.364883092451105 70-71 20.796884047087445 29.08797663141459 30.55902144048605 19.556117881011907 72-73 19.78236737431496 28.21731637015057 31.88237841142086 20.117937844113616 74-75 17.885446226783802 32.09145440024469 29.269507553229452 20.753591819742052 76-77 17.99119596393991 29.905130479470802 30.977866612954568 21.125806943634718 78-79 18.23544498589382 30.188448808607827 30.798787394123988 20.777318811374364 80-81 20.223174744859378 29.89588468827231 29.46119967967785 20.419740887190457 82-83 19.36029661127142 28.71940970705603 31.96001309827902 19.960280583393526 84-85 18.39489718303654 29.617846058793653 31.04870064899874 20.93855610917107 86-87 17.55073278179027 31.168497626199837 31.21943868262183 20.061330909388065 88-89 18.377265776641703 30.00625773924176 29.348584737166988 22.26789174694955 90-91 18.696653454897184 28.12466488362636 31.25785034990131 21.920831311575146 92-93 20.619363830571526 30.82717110371986 29.933510595918854 18.61995446978976 94-95 19.425055194319047 28.41851683121423 31.14064011604878 21.015787858417944 96-97 17.831606510466283 28.745855863935947 32.68394604012442 20.738591585473355 98-99 18.660017182668533 30.48427212719632 29.767566996752908 21.08814369338223 100-101 17.912648454544684 28.482951603181743 31.206174605465336 22.39822533680824 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 185.0 1 120.5 2 90.0 3 151.5 4 129.5 5 99.0 6 210.0 7 391.0 8 486.5 9 471.5 10 391.0 11 332.5 12 335.0 13 342.5 14 357.5 15 389.0 16 421.5 17 439.5 18 468.0 19 535.0 20 672.0 21 808.0 22 967.0 23 1186.5 24 1379.5 25 1748.5 26 2596.0 27 4206.5 28 5784.5 29 8591.5 30 14811.0 31 21918.5 32 35896.0 33 49795.5 34 59455.5 35 67234.5 36 74507.0 37 76522.5 38 64015.5 39 53782.5 40 44657.0 41 34322.5 42 27444.0 43 24681.0 44 24923.0 45 23423.5 46 21117.5 47 30460.0 48 56305.0 49 51784.5 50 22391.0 51 8793.0 52 4299.5 53 2947.5 54 2285.0 55 1830.0 56 1476.5 57 1211.5 58 1169.0 59 1101.0 60 900.5 61 735.0 62 605.5 63 459.0 64 305.0 65 224.0 66 166.5 67 165.0 68 144.0 69 78.5 70 45.0 71 37.0 72 46.0 73 56.5 74 56.0 75 46.0 76 30.5 77 23.5 78 22.5 79 18.5 80 13.5 81 8.5 82 5.0 83 1.5 84 2.0 85 2.5 86 1.5 87 0.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4894173441447021 2 0.3775353727313412 3 0.20626581459619622 4 0.23500516459905954 5 0.25907834707378274 6 0.20743235647823127 7 0.19990285887600506 8 0.19958471108999554 9 0.19937261256598918 10-11 0.21289389347139534 12-13 0.200433105186021 14-15 0.210985006755338 16-17 0.09517921264785918 18-19 0.03913217767917553 20-21 2.1209852400637147E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.14242415887027843 46-47 0.21337111515040966 48-49 0.08711946873561707 50-51 0.06336443404690346 52-53 0.07243164594817585 54-55 0.08155188248044981 56-57 0.11750258229952977 58-59 0.0943838431828353 60-61 0.14009107510620833 62-63 0.24423145039333669 64-65 0.13340997160000764 66-67 0.10636740978919527 68-69 0.11013215859030838 70-71 0.08892230618967122 72-73 0.07232559668617267 74-75 0.1457116859923772 76-77 0.22689239605581585 78-79 0.14311347907329913 80-81 0.08287749825548964 82-83 0.09008884807170627 84-85 0.10270871025008536 86-87 0.07343911393720612 88-89 0.0980425427219452 90-91 0.1188812227055712 92-93 0.17020906551511308 94-95 0.13627330167409363 96-97 0.08627107463959158 98-99 0.07736293663132399 100-101 0.06172067048585409 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 942958.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.978110948327187 #Duplication Level Percentage of deduplicated Percentage of total 1 73.84012203840999 11.059855403293243 2 9.789472790727213 2.932556191702847 3 3.917164067337516 1.7601515401014571 4 2.122856654066704 1.2718552996802288 5 1.3979399517971154 1.0469249848558178 6 0.9751534855645392 0.8763574259060186 7 0.7354480328374542 0.7710935562797855 8 0.5841731066105876 0.6999847683073878 9 0.47968331933652003 0.6466274979375831 >10 4.3023774743903145 13.90675294592954 >50 0.8313071489931859 8.822193854732909 >100 0.8720504916351562 26.550625909918068 >500 0.10007136789255892 10.387131690885319 >1k 0.04860609297638576 13.874062003921365 >5k 0.0021443864548405483 2.045435955373319 >10k+ 0.0014295909698936989 3.348390971175114 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 19589 2.077398993380405 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11686 1.2392916757692283 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7853 0.8328048545110175 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6166 0.6538997495116432 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5086 0.5393665465482026 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4695 0.497901285104957 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4635 0.49153832938476577 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 4494 0.47658538344231666 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4271 0.4529363980156062 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3805 0.4035174419221217 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3755 0.3982149788219624 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3503 0.3714905647971596 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 3457 0.36661229874501305 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 3268 0.34656898822641097 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 3159 0.3350096186680637 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2933 0.31104248545534374 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2772 0.2939685542728308 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 2645 0.28050029799842624 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2636 0.27954585464039755 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2558 0.27127401220414904 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 2443 0.25907834707378274 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2391 0.25356378544961705 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2356 0.24985206127950557 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2051 0.2175070363685339 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1984 0.21040173581432048 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 1899 0.2013875485440497 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 1859 0.19714557806392224 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1786 0.1894039819376897 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1774 0.18813139079365146 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 1733 0.18378337105152087 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 1732 0.18367732178951765 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1691 0.17932930204738706 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1674 0.17752646459333288 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1669 0.17699621828331696 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 1623 0.17211795223117043 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1609 0.17063326256312583 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 1588 0.16840622806105893 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1559 0.16533079946296655 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1545 0.16384610979492195 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 1539 0.16320981422290282 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1538 0.16310376496089962 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1522 0.16140697676884866 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1494 0.15843759743275945 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1458 0.15461982400064478 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 1453 0.15408957769062884 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1424 0.15101414909253646 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1346 0.14274230665628798 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 1333 0.14136366625024657 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 1332 0.14125761698824338 No Hit TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA 1328 0.14083341994023063 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 1297 0.1375458928181319 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1272 0.13489466126805225 No Hit GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 1249 0.13245552824197895 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 1242 0.13171318340795668 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 1234 0.13086478931193118 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1226 0.1300163952159057 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 1224 0.1298042966918993 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 1209 0.12821355776185153 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1180 0.12513812916375913 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 1162 0.12322924244770181 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 1148 0.1217445527796572 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 1133 0.12015381384960942 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1128 0.11962356753959348 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1121 0.1188812227055712 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 1100 0.1166541882035043 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1065 0.1129424640333928 No Hit ACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTAT 1058 0.11220011919937048 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1052 0.11156382362735137 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1045 0.11082147879332908 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 1044 0.11071542953132588 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 1036 0.10986703543530041 No Hit TATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGC 1030 0.1092307398632813 No Hit ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT 1014 0.10753395167123032 No Hit GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC 999 0.10594321274118254 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 988 0.10477667085914749 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 983 0.10424642454913155 No Hit GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG 981 0.10403432602512519 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 977 0.10361012897711244 No Hit GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG 976 0.10350407971510926 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 974 0.10329198119110289 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 974 0.10329198119110289 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 966 0.10244358709507742 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 962 0.10201939004706466 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 951 0.10085284816502961 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 950 0.10074679890302643 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.1209852400637144E-4 0.0 10-11 0.0 0.0 0.0 4.7722167901433576E-4 0.0 12-13 0.0 0.0 0.0 7.423448340223E-4 0.0 14-15 0.0 0.0 0.0 0.0012195665130366358 0.0 16-17 0.0 0.0 0.0 0.003128453229093979 0.0 18-19 0.0 0.0 0.0 0.004507093635135393 0.0 20-21 0.0 0.0 0.0 0.008006719281240523 0.0 22-23 0.0 0.0 0.0 0.0155892415144683 0.0 24-25 0.0 0.0 0.0 0.024550404153737493 0.0 26-27 0.0 0.0 0.0 0.0319208278629589 0.0 28-29 0.0 0.0 0.0 0.04300297574229181 0.0 30-31 0.0 0.0 0.0 0.06400072961892259 0.0 32-33 0.0 0.0 0.0 0.11039728174531634 0.0 34-35 0.0 0.0 0.0 0.17503430693625804 0.0 36-37 0.0 0.0 0.0 0.25488940122465686 0.0 38-39 0.0 0.0 0.0 0.3831029589865084 0.0 40-41 0.0 0.0 0.0 0.5375106844631468 0.0 42-43 0.0 0.0 0.0 0.6744202817092595 0.0 44-45 0.0 0.0 0.0 0.83455466733407 0.0 46-47 0.0 0.0 0.0 1.0467592406024446 0.0 48-49 0.0 0.0 0.0 1.2379660599941884 0.0 50-51 0.0 0.0 0.0 1.4391945346452335 0.0 52-53 0.0 0.0 0.0 1.639309492045245 0.0 54-55 0.0 0.0 0.0 1.835076429703126 0.0 56-57 0.0 0.0 0.0 2.0953213186589434 0.0 58-59 0.0 0.0 0.0 2.3545587396257313 0.0 60-61 0.0 0.0 0.0 2.6317715105020585 0.0 62-63 0.0 0.0 0.0 2.8944025078529476 0.0 64-65 0.0 0.0 0.0 3.1576698007758566 0.0 66-67 0.0 0.0 0.0 3.4801125818965426 0.0 68-69 0.0 0.0 0.0 3.843808525936468 0.0 70-71 0.0 0.0 0.0 4.251620962969719 0.0 72-73 0.0 0.0 0.0 4.621361714943825 0.0 74-75 0.0 0.0 0.0 5.0034041813103025 0.0 76-77 0.0 0.0 0.0 5.391862628027972 0.0 78-79 0.0 0.0 0.0 5.942788544134521 0.0 80-81 0.0 0.0 0.0 6.526377632938052 0.0 82-83 0.0 0.0 0.0 7.055298327178941 0.0 84-85 0.0 0.0 0.0 7.563645464591212 0.0 86-87 0.0 0.0 0.0 8.074166611874547 0.0 88-89 0.0 0.0 0.0 8.648317316359794 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGAAA 25 0.0050238287 56.991875 9 GGATGCC 100 1.2732926E-11 47.49323 8 ATGTATT 155 0.0 46.10322 1 GGGATGC 125 3.6379788E-12 41.79404 7 GTATGGT 135 7.2759576E-12 38.72287 3 CATGGAT 150 0.0 38.018814 4 TGGGAGT 130 2.055458E-10 36.53519 6 GAGTTAA 130 2.055458E-10 36.533253 9 CAAGTAC 80 1.0558844E-5 35.73 1 CTAGAGA 55 0.0025731346 34.56256 4 TGGGTAT 180 0.0 34.302486 6 CATGGGT 335 0.0 34.046703 4 CTATACA 385 0.0 32.093807 4 TATTGTC 60 0.0039533507 31.662151 7 ACGTGAA 60 0.0039533507 31.662151 8 CATGGGG 665 0.0 31.444134 4 TACAATC 395 0.0 31.261366 7 GATGACC 340 0.0 30.730911 9 ACTATAC 500 0.0 30.415054 3 CATATAG 190 1.0913936E-11 30.088419 1 >>END_MODULE