FastQCFastQC Report
Fri 10 Feb 2017
SRR2031615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942958
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT220892.3425221483883694No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT105911.12316773387574No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT74350.7884762629936858No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC56350.5975875913879515No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC51540.5465778963644192No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT47980.5088243590912851No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT47350.5021432555850844No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT47310.5017190585370717No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC45700.4846451273545587No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA43210.45823886111576545No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG38840.41189533362037334No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC35900.38071685059143673No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC34440.36523365833897165No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC32890.3487960227284778No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA32670.3464629389644077No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA30220.3204808697736272No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA29070.30828520464326087No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC28170.2987407710629742No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT26750.2836817758585218No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC25770.2732889481822096No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC25700.27254660334818726No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG22030.23362652419301813No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA20770.22026431718061676No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT20640.21888567677457532No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19220.20382668157012296No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC18450.19566088839587767No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA18400.19513064208586173No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG18380.19491854356185537No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT18270.19375200167982032No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA18220.19322175536980438No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC18190.19290360758379482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18100.19194916422576616No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC17710.1878132430076419No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG17020.18049584392942208No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC16790.17805671090334882No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC16730.1774204153313297No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT16370.17360264189921504No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT15890.16851227732306212No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT15560.165012651676957No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA15490.16427030684293467No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC15410.1634219127469092No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC15090.16002833636280725No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC15070.1598162378388009No Hit
GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG14450.15324118359460334No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC14020.1486810653284664No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT13870.1470903263984186No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG13450.1426362573942848No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA13210.14009107510620833No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC13200.13998502584420514No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT12930.13712169577011912No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA12830.1360612031500873No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC12650.13415231643402994No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT12610.1337281193860172No Hit
TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA12590.1335160208620108No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT12540.1329857745519949No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG12470.1322434297179726No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC12430.13181923266995985No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA11920.12641072030779738No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT11920.12641072030779738No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG11790.12503207990175597No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11760.12471393211574641No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT11660.12365343949571454No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC11500.12195665130366358No Hit
ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA11470.12163850351765403No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA11300.11983566606359987No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA11260.11941146901558714No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT11100.11771468082353614No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG11020.11686628672751065No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT10730.11379085812941826No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC10690.11336666108140553No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT10650.1129424640333928No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA10640.1128364147713896No Hit
ACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTAT10510.11145777436534819No Hit
GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG10360.10986703543530041No Hit
GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG10180.10795814871924306No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG10140.10753395167123032No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT10110.10721580388522076No Hit
ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC9890.10488272012115067No Hit
TATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGC9700.10286778414309015No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA9520.10095889742703282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAG1750.043.4241373
ATGTATT1350.042.247031
TGGGTAT2050.039.386226
GAGTTAA756.925815E-637.9961179
GACATAT1900.037.5220341
CATGGGG5900.037.0301134
GATGACC3150.036.1867839
GGGGAGC1054.660251E-836.186787
TAACTTT11700.035.7228476
TGGACAG550.002580684134.5419245
GGACAGA550.002580684134.5419246
TACAATC3350.034.0263757
GCTGTAC702.0459804E-433.9485051
ACTTTAT12750.033.89858
ATAGACC851.6330341E-533.525993
CTATACA3700.033.374974
ATACTCA2900.032.7552766
TCTAGAC2350.032.337123
GGATATG2650.032.2831081
ACTCAAT2800.032.228858
Produced by FastQC (version 0.11.5)