##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031615_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 942958 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.496329635042066 34.0 31.0 34.0 31.0 34.0 2 32.6942324048367 34.0 31.0 34.0 31.0 34.0 3 32.803983846576415 34.0 31.0 34.0 31.0 34.0 4 36.21200201917795 37.0 35.0 37.0 35.0 37.0 5 35.98486570981952 37.0 35.0 37.0 35.0 37.0 6 36.08631031286653 37.0 37.0 37.0 35.0 37.0 7 36.02951244912287 37.0 37.0 37.0 35.0 37.0 8 36.132565819474465 37.0 37.0 37.0 35.0 37.0 9 37.999513233887406 39.0 39.0 39.0 35.0 39.0 10-11 37.95443858581188 39.0 38.0 39.0 35.0 39.0 12-13 38.01330175893305 39.0 39.0 39.0 35.0 39.0 14-15 39.36856996812159 41.0 39.0 41.0 36.0 41.0 16-17 39.39581508402283 41.0 39.0 41.0 36.0 41.0 18-19 39.48007652514746 41.0 39.5 41.0 37.0 41.0 20-21 39.505591447339114 41.0 39.0 41.0 37.0 41.0 22-23 39.41303271195536 41.0 39.0 41.0 36.0 41.0 24-25 39.32710576717096 41.0 39.0 41.0 36.0 41.0 26-27 39.18191584354764 41.0 39.0 41.0 36.0 41.0 28-29 39.15814012925284 41.0 39.0 41.0 36.0 41.0 30-31 39.08175231558563 40.0 39.0 41.0 35.5 41.0 32-33 38.919590268071325 40.0 38.0 41.0 35.0 41.0 34-35 38.77142248117096 40.0 38.0 41.0 35.0 41.0 36-37 38.616707743080816 40.0 38.0 41.0 34.5 41.0 38-39 38.48705668757251 40.0 38.0 41.0 34.5 41.0 40-41 38.51428006337504 40.0 38.0 41.0 35.0 41.0 42-43 38.39510190273586 40.0 38.0 41.0 34.0 41.0 44-45 38.32209706052656 40.0 38.0 41.0 34.0 41.0 46-47 38.292705507562374 40.0 38.0 41.0 34.0 41.0 48-49 38.36127855111256 40.0 38.5 41.0 34.0 41.0 50-51 38.25011400295665 40.0 38.0 41.0 34.0 41.0 52-53 38.310650633432246 40.0 38.0 41.0 34.0 41.0 54-55 38.26629871107727 40.0 38.0 41.0 34.0 41.0 56-57 38.139373121602446 40.0 38.0 41.0 34.0 41.0 58-59 37.999988334581175 40.0 38.0 41.0 33.0 41.0 60-61 37.681419533001474 40.0 38.0 41.0 32.5 41.0 62-63 37.582027513420535 40.0 37.0 41.0 32.5 41.0 64-65 37.3543296732198 39.5 37.0 41.0 32.0 41.0 66-67 37.04733190661726 39.0 36.5 41.0 32.0 41.0 68-69 36.70047499464451 39.0 36.0 41.0 31.5 41.0 70-71 36.257810528146535 38.5 35.0 40.0 31.0 41.0 72-73 35.85787065807809 37.0 35.0 39.5 30.5 41.0 74-75 35.49968662443078 37.0 35.0 39.0 31.0 41.0 76-77 34.84372951923628 36.0 34.0 39.0 30.0 40.0 78-79 34.74908744610046 36.0 35.0 38.0 30.0 39.5 80-81 34.38502775309186 35.5 34.0 37.0 30.0 39.0 82-83 34.02427043410205 35.0 34.0 37.0 29.5 39.0 84-85 33.67770356686088 35.0 34.0 36.0 29.0 38.0 86-87 33.51260130355753 35.0 34.0 36.0 29.0 37.0 88-89 33.24480517690077 35.0 34.0 36.0 29.0 37.0 90-91 33.05140578901711 35.0 34.0 35.0 29.0 36.5 92-93 32.91862628027972 35.0 34.0 35.0 29.0 36.0 94-95 32.74275206318839 35.0 34.0 35.0 29.0 36.0 96-97 32.60823705827831 35.0 34.0 35.0 28.0 36.0 98-99 32.6250448058132 35.0 34.0 35.0 29.0 35.5 100-101 32.21051839000253 34.5 33.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16949951685506193 1101 2 0.08079175297327623 1101 3 0.16240462357382057 1101 4 0.027524474089098305 1101 5 0.2070292740012789 1101 6 0.15550088494766356 1101 7 0.23641494857985634 1101 8 0.18181405765714942 1101 9 0.26661058576590335 1101 10-11 0.03748395358499579 1101 12-13 0.10075889175043784 1101 14-15 0.058492960256046445 1101 16-17 0.14587602821814727 1101 18-19 0.22533261122901393 1101 20-21 0.1177241482611251 1101 22-23 0.1329480850823046 1101 24-25 0.10332887017914771 1101 26-27 0.24654403802210112 1101 28-29 0.16794497445834367 1101 30-31 0.15056461689486156 1101 32-33 -0.058038105626998515 1101 34-35 0.014416551043709092 1101 36-37 0.11559598642163138 1101 38-39 0.11039031349379513 1101 40-41 0.03292878610857741 1101 42-43 0.10566066916091188 1101 44-45 0.2425996142050053 1101 46-47 0.12342758831564282 1101 48-49 -0.18513166619365506 1101 50-51 0.08503223225129375 1101 52-53 -0.20164875009168526 1101 54-55 -0.014470139711221464 1101 56-57 -0.003916961700213051 1101 58-59 -0.16828732711871908 1101 60-61 -0.45368705780385454 1101 62-63 -0.45263299707566773 1101 64-65 -0.08117625723552635 1101 66-67 -0.10525632688234765 1101 68-69 -0.16821074855706541 1101 70-71 -0.13305159913721099 1101 72-73 -0.1479620861684765 1101 74-75 -0.27336649361959076 1101 76-77 -0.36078868400492325 1101 78-79 -0.2796930206158379 1101 80-81 -0.11619112140078158 1101 82-83 -0.07721186037034755 1101 84-85 -0.20999522611506194 1101 86-87 -0.2991166543988797 1101 88-89 -0.20200830058763586 1101 90-91 -0.07028049769075295 1101 92-93 -0.40767449548555845 1101 94-95 -0.12113580296747983 1101 96-97 -0.25386679185076133 1101 98-99 -0.47009060746907494 1101 100-101 -0.28998227671227284 1102 1 0.1664753087722417 1102 2 0.14422082285807392 1102 3 0.178470053162755 1102 4 0.1149273317674897 1102 5 0.07830037857615935 1102 6 0.1252551995806499 1102 7 0.10807534334895053 1102 8 0.004153547542813385 1102 9 0.14821590318158684 1102 10-11 0.1617828707815292 1102 12-13 0.08464840542605856 1102 14-15 0.25797895301813867 1102 16-17 0.34583241329823267 1102 18-19 0.2911785244028806 1102 20-21 0.108709196634031 1102 22-23 0.14469527714655328 1102 24-25 0.018041876797077805 1102 26-27 0.21037429307926914 1102 28-29 -0.18413556535738707 1102 30-31 -0.19267398915788903 1102 32-33 0.1640014866275692 1102 34-35 0.20725757646291498 1102 36-37 0.2486845715680417 1102 38-39 0.037153293438144885 1102 40-41 -0.04342616003960842 1102 42-43 0.022109261228045796 1102 44-45 -0.09491858871611214 1102 46-47 0.10064149606828465 1102 48-49 0.06220277733763169 1102 50-51 0.20280332961709036 1102 52-53 0.0391020032569358 1102 54-55 -0.03205848695807134 1102 56-57 -0.0262849274426884 1102 58-59 0.05052440060882901 1102 60-61 0.38788444903756414 1102 62-63 0.1709846622809934 1102 64-65 0.22598812415351688 1102 66-67 0.47078502438716896 1102 68-69 0.41472608550439105 1102 70-71 0.3229225635284152 1102 72-73 0.4275602115087622 1102 74-75 0.4411738555881328 1102 76-77 0.458261664193536 1102 78-79 0.31152343915029945 1102 80-81 0.33811413978410343 1102 82-83 0.14988489987014475 1102 84-85 0.29323263841194347 1102 86-87 0.2675060368939981 1102 88-89 0.06751027720552116 1102 90-91 0.08836246552853311 1102 92-93 0.21773281740283323 1102 94-95 0.37599318185019825 1102 96-97 0.4362257727065284 1102 98-99 0.32889507530266826 1102 100-101 0.1517215167043986 1103 1 0.10242719612348594 1103 2 0.12308571974030968 1103 3 0.13989848997283616 1103 4 0.09959428562027028 1103 5 -0.04189037582586508 1103 6 0.13783193212063338 1103 7 0.16171744662825915 1103 8 0.09592970391565103 1103 9 0.07338821485031843 1103 10-11 0.10245481898033404 1103 12-13 -0.001822099662526 1103 14-15 0.19487148707661106 1103 16-17 0.211735568755671 1103 18-19 0.19832037190705165 1103 20-21 0.15513911424751115 1103 22-23 0.07913270647183879 1103 24-25 -0.02077199967699528 1103 26-27 0.09624641897448072 1103 28-29 0.08152393063097918 1103 30-31 0.01810323070103692 1103 32-33 0.05945171817137407 1103 34-35 0.006568329787988603 1103 36-37 -0.010344963728925904 1103 38-39 0.010227912467811962 1103 40-41 0.06576794636284689 1103 42-43 0.14052809973970426 1103 44-45 0.09824043610753108 1103 46-47 -0.03032715128512109 1103 48-49 -0.014265573855084313 1103 50-51 0.08636001746373978 1103 52-53 -0.005885813769609172 1103 54-55 -0.010852709132429084 1103 56-57 0.04147865556308261 1103 58-59 0.08326698312996683 1103 60-61 0.04417315185377646 1103 62-63 0.13653074219052996 1103 64-65 0.32209058228716714 1103 66-67 0.1393593472561605 1103 68-69 0.24535078261278187 1103 70-71 0.3864086439764307 1103 72-73 0.3788131731155673 1103 74-75 0.24735183469534405 1103 76-77 0.13178826480739048 1103 78-79 0.25957248066664107 1103 80-81 0.357950174461827 1103 82-83 0.24839871172734718 1103 84-85 0.04560865478155307 1103 86-87 0.12817926396846957 1103 88-89 0.2993822146348464 1103 90-91 0.32066686486081863 1103 92-93 0.11989882892617487 1103 94-95 0.22981769318507617 1103 96-97 0.3720009732880811 1103 98-99 0.3185092587068965 1103 100-101 0.3338990381087399 1104 1 0.2165529311632639 1104 2 0.14418568515020525 1104 3 0.10264926825024645 1104 4 0.06699310955661986 1104 5 0.04702781857053395 1104 6 0.12881688542398706 1104 7 0.1355370592190539 1104 8 0.17172527638192747 1104 9 0.19760993743766164 1104 10-11 0.13515327972056213 1104 12-13 0.14565066062803567 1104 14-15 0.2268565268552365 1104 16-17 0.16730889978300212 1104 18-19 0.10206258913292032 1104 20-21 0.024907360529077494 1104 22-23 0.08321866288831359 1104 24-25 0.14277435106669145 1104 26-27 0.21375549887763157 1104 28-29 0.03791877670500554 1104 30-31 0.1893783085287737 1104 32-33 0.18729459260928394 1104 34-35 0.2288097687364541 1104 36-37 0.11977869590442225 1104 38-39 0.12611558109458798 1104 40-41 0.017914694892766647 1104 42-43 0.15915271060099911 1104 44-45 0.2969930303655772 1104 46-47 0.36450811332920097 1104 48-49 0.30728190106708553 1104 50-51 0.3846888829082218 1104 52-53 0.3918031346911306 1104 54-55 0.490595751607799 1104 56-57 0.37209522422443797 1104 58-59 0.17110423667545405 1104 60-61 0.3449557530298435 1104 62-63 0.2658436961856907 1104 64-65 0.2214441796583131 1104 66-67 -0.03526664720942563 1104 68-69 -0.09061644326062179 1104 70-71 0.08002676919261376 1104 72-73 0.13133176529819934 1104 74-75 0.1458190605687406 1104 76-77 -0.05943017171976095 1104 78-79 0.18218792514952042 1104 80-81 0.14578310424391105 1104 82-83 0.1919303616754604 1104 84-85 0.22923888653527769 1104 86-87 0.1258228129134693 1104 88-89 0.13677628243146245 1104 90-91 0.048485958600018364 1104 92-93 0.22985213228141532 1104 94-95 0.16604218298099482 1104 96-97 0.04234644198403714 1104 98-99 0.13185239257062165 1104 100-101 0.1307794428129938 1105 1 0.14119270232596648 1105 2 0.1413430226614807 1105 3 0.03688228349024314 1105 4 0.13683418157864935 1105 5 0.09303859576277063 1105 6 0.07783293252079204 1105 7 0.1193504998495456 1105 8 0.19651994001009143 1105 9 0.2788453977234724 1105 10-11 0.16858126956056196 1105 12-13 0.13937187792851802 1105 14-15 0.18201634501978958 1105 16-17 0.23060561677487357 1105 18-19 0.10978495774138963 1105 20-21 0.22372905022190537 1105 22-23 0.16264110983318858 1105 24-25 0.23170399071916137 1105 26-27 0.19507344978680408 1105 28-29 0.1400098249887236 1105 30-31 0.13992696019283102 1105 32-33 0.2104246073270346 1105 34-35 0.2846170492757878 1105 36-37 0.5315092779677499 1105 38-39 0.47011186604924404 1105 40-41 0.35487108761934394 1105 42-43 0.3405831217485016 1105 44-45 0.4683509803252264 1105 46-47 0.4486144253455322 1105 48-49 0.5079433096983337 1105 50-51 0.5629131387122399 1105 52-53 0.5114961390115056 1105 54-55 0.526212116797943 1105 56-57 0.49178377761190006 1105 58-59 0.44045986028083206 1105 60-61 0.5220643083163665 1105 62-63 0.5805958682409553 1105 64-65 0.6058415826873258 1105 66-67 0.5265642292089439 1105 68-69 0.6096339958981005 1105 70-71 0.5489236499788248 1105 72-73 0.714059771755025 1105 74-75 0.6138984533427987 1105 76-77 0.33220593187422054 1105 78-79 0.401016558297691 1105 80-81 0.44303420807527516 1105 82-83 0.5331626172895696 1105 84-85 0.506705593557065 1105 86-87 0.48580981618935226 1105 88-89 0.5565600857833104 1105 90-91 0.5335145039164431 1105 92-93 0.5310007697024872 1105 94-95 0.6002158524487768 1105 96-97 0.6584905691264211 1105 98-99 0.474621703684889 1105 100-101 0.6393712269842879 1106 1 0.1402595308922585 1106 2 0.15114694423010633 1106 3 0.13597834393276997 1106 4 0.13493410962326635 1106 5 0.09690619784947785 1106 6 -0.018002649418406236 1106 7 0.09741534964267373 1106 8 0.1512330179017951 1106 9 0.12934683683122472 1106 10-11 0.1949293087762527 1106 12-13 0.11338923716578364 1106 14-15 0.10416398848084896 1106 16-17 0.14690688498858862 1106 18-19 0.09577052169174038 1106 20-21 0.050828123796286206 1106 22-23 0.10165891879163524 1106 24-25 0.02889098478284069 1106 26-27 0.20048134689059793 1106 28-29 0.13871125510205218 1106 30-31 0.07758998411799922 1106 32-33 0.17889915310865234 1106 34-35 0.11759622718548002 1106 36-37 0.16545310779326172 1106 38-39 0.2071655177851639 1106 40-41 0.10403910344953005 1106 42-43 0.22816969307428536 1106 44-45 0.06659257053929224 1106 46-47 -0.08308529582733826 1106 48-49 0.06553572739936442 1106 50-51 0.036199701043592825 1106 52-53 -0.14349464170445714 1106 54-55 0.018589342917749718 1106 56-57 0.01881202010155647 1106 58-59 -0.1357229064038208 1106 60-61 -0.07292804642369788 1106 62-63 -0.09958491967054073 1106 64-65 0.07651976221601586 1106 66-67 0.07128337833648146 1106 68-69 0.012640381938759049 1106 70-71 0.10278900345966946 1106 72-73 0.15308882374279165 1106 74-75 0.024623179012891683 1106 76-77 -0.06303714241972358 1106 78-79 0.048019166680496994 1106 80-81 0.15259303160468818 1106 82-83 0.2002452760411444 1106 84-85 0.007565685300185976 1106 86-87 0.05409435477543667 1106 88-89 0.0741610481866175 1106 90-91 0.0824273029810243 1106 92-93 0.05612331872209353 1106 94-95 -0.051520071278510216 1106 96-97 -0.10806479154995685 1106 98-99 -0.13066926591395855 1106 100-101 -0.011021154739047745 1107 1 0.1307520735604939 1107 2 0.19570001685580252 1107 3 0.13124639785107206 1107 4 0.11321302920708831 1107 5 0.07522996393014836 1107 6 0.19748733059287105 1107 7 0.25468432611748426 1107 8 0.1780896628713009 1107 9 0.236952802696635 1107 10-11 0.19178685067569745 1107 12-13 0.1278317311435444 1107 14-15 0.27520083948797236 1107 16-17 0.23106774296613963 1107 18-19 0.2383977576099383 1107 20-21 0.1784317832704332 1107 22-23 0.25569894749339284 1107 24-25 0.2869537442518606 1107 26-27 0.2334621013368121 1107 28-29 0.15700990714449148 1107 30-31 0.10924520425023587 1107 32-33 0.2672038273949582 1107 34-35 0.19113679404520667 1107 36-37 0.20915537830733655 1107 38-39 0.30071146622450584 1107 40-41 0.3474127132081364 1107 42-43 0.3482859095675437 1107 44-45 0.17787146449654756 1107 46-47 0.30450567849483434 1107 48-49 0.13023833105883398 1107 50-51 0.31672927410494367 1107 52-53 0.37345733410406723 1107 54-55 0.3993841509747398 1107 56-57 0.34014613931636006 1107 58-59 0.3908473616500956 1107 60-61 0.43423842377260513 1107 62-63 0.29882623301255506 1107 64-65 0.23757698634599933 1107 66-67 0.17966903258280809 1107 68-69 0.2873834755782383 1107 70-71 0.1236278892164222 1107 72-73 0.20949738814235985 1107 74-75 0.32847357109940134 1107 76-77 0.12859450126845928 1107 78-79 0.14811782897356096 1107 80-81 0.22361258467731204 1107 82-83 0.2948643370836379 1107 84-85 0.5203578281939372 1107 86-87 0.22853697688604058 1107 88-89 0.17320005182558873 1107 90-91 0.235240459661874 1107 92-93 0.38349762039807445 1107 94-95 0.3207109778732544 1107 96-97 0.28844935431646945 1107 98-99 0.38757492966801976 1107 100-101 0.559019463373815 1108 1 0.09718404680302228 1108 2 0.02885844097630752 1108 3 0.07270594050185508 1108 4 0.0185039319082847 1108 5 -0.1354342467364873 1108 6 0.00299030566527847 1108 7 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-1.1288431386140942 2316 86-87 -0.8058519951278811 2316 88-89 -0.5837468888290474 2316 90-91 -0.6337260650528194 2316 92-93 -0.717252275572676 2316 94-95 -0.7316259175397235 2316 96-97 -0.6243170269706582 2316 98-99 -0.814430842848779 2316 100-101 -0.84154627868676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 17.0 14 69.0 15 247.0 16 651.0 17 1507.0 18 2351.0 19 2847.0 20 3850.0 21 5038.0 22 5171.0 23 3972.0 24 3596.0 25 3721.0 26 4527.0 27 5650.0 28 7279.0 29 8807.0 30 10517.0 31 13199.0 32 16643.0 33 22121.0 34 30621.0 35 45948.0 36 80507.0 37 177933.0 38 355882.0 39 130220.0 40 66.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.09380752560967 25.199492846267695 15.351798114618864 20.354901513503766 2 12.68085702074679 26.26049774057974 40.35554716572935 20.70309807294412 3 18.06323532218963 30.563633008609116 33.21243666180959 18.160695007391663 4 9.274555346972543 19.54956674833222 47.469534506347635 23.7063433983476 5 8.306838692709539 44.37673788228108 38.18674850841713 9.129674916592254 6 22.922017735678576 46.2117082627222 18.94570065686913 11.920573344730094 7 22.50694092419811 34.97080463817052 28.17315299302832 14.349101444603047 8 21.21727584897737 35.910719247304755 23.643258766562244 19.228746137155632 9 23.250134152316434 19.102123318323827 26.612744151913446 31.034998377446293 10-11 20.86280619073172 27.84466540397346 33.66342933619525 17.629099069099578 12-13 23.631063101431877 27.674191215303335 30.61408885655565 18.080656826709145 14-15 20.595933222900705 30.196891059835114 30.239522863160396 18.96765285410379 16-17 17.587739856918336 32.79361328924512 30.70937411846551 18.909272735371037 18-19 16.906214274654864 34.57333200418258 31.251497945825797 17.268955775336757 20-21 19.125878353012542 31.46380856835617 29.93733549108232 19.472977587548968 22-23 18.81564916481828 30.857420711955236 30.365129857355488 19.961800265871 24-25 21.120028675720445 31.017182101429757 29.174682223386405 18.68810699946339 26-27 19.491430159137522 29.47167318162633 33.49062206376106 17.54627459547509 28-29 18.120325913841686 31.77872098493314 32.00894815195099 18.09200494927418 30-31 19.621657885176827 30.346707537938954 30.032377048093572 19.999257528790647 32-33 18.815047966081206 32.05810863262202 30.850366612298746 18.276476788998025 34-35 20.173114815294 30.304637110030352 31.41343516890466 18.10881290577099 36-37 20.3695180485239 28.87318417151135 31.887581419320902 18.869716360643846 38-39 18.388358760411386 32.178315471102636 31.914889104286726 17.518436664199257 40-41 17.59887502942867 31.678240176126614 32.53623173036339 18.186653064081327 42-43 17.782128154170177 31.4746786177115 28.807645727593385 21.935547500524944 44-45 19.21967892525436 30.27732942506453 29.93415401322222 20.568837636458888 46-47 20.456584492628515 30.630367418273135 31.117345629391764 17.795702459706582 48-49 18.82439059321517 30.66328600943649 31.14492357716677 19.367399820181568 50-51 16.806315818501353 30.439714521779432 32.272795249241916 20.481174410477294 52-53 18.615675353515215 31.69329917133107 29.9966170285421 19.694408446611618 54-55 18.050678002679877 29.737097322402427 30.53681862361187 21.675406051305817 56-57 20.158480017137563 29.152411878365736 29.518281832276728 21.170826272219973 58-59 21.402968106744947 28.894871245591002 29.222139268132835 20.48002137953122 60-61 20.249534045808005 31.41080059281569 30.41563379049427 17.924031570882036 62-63 17.97667552531502 31.34588178900863 31.636403742266356 19.041038943409994 64-65 17.578089374076043 32.34391139372061 29.750794839218713 20.32720439298463 66-67 19.356111300821457 30.000912023653225 28.731555382106094 21.91142129341922 68-69 18.053190067850316 30.96065784478206 29.51531245294064 21.470839634426984 70-71 20.643231193754122 29.24573522892854 30.459575081817004 19.65145849550033 72-73 19.78221723555026 28.33397669885615 31.63422973239529 20.249576333198295 74-75 17.752805533226294 32.488032340782944 28.80796387537939 20.951198250611373 76-77 17.96463893407766 30.150653581601727 30.506448855622416 21.3782586286982 78-79 18.22726993142855 30.491283811155956 30.24111360209044 21.04033265532505 80-81 20.106591151343526 30.07763654008305 29.07308122669976 20.74269108187367 82-83 19.2302144844906 28.911870885087897 31.617001374465488 20.240913255956016 84-85 18.01856498380628 29.90875521497246 30.748877468561698 21.323802332659568 86-87 17.28152261288414 31.56667635249926 30.511857367984575 20.639943666632025 88-89 18.04025205788593 30.342814844351494 28.7484702393956 22.86846285836697 90-91 18.45835127333349 28.330105900793036 30.732598906844206 22.478943919029266 92-93 20.289503880342497 31.139934122198444 29.329779269066066 19.240782728392993 94-95 19.02163192846341 28.81660689023265 30.749513764133717 21.412247417170224 96-97 17.329403854678574 29.15522218380882 32.26156414177514 21.253809819737466 98-99 18.154255014539352 30.877462198740556 29.264399899041106 21.703882887678986 100-101 17.20331128215679 29.115612784450633 30.652054492352786 23.02902144103979 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 146.0 1 99.0 2 76.5 3 148.5 4 130.0 5 88.0 6 166.5 7 326.0 8 464.5 9 501.0 10 404.5 11 287.5 12 283.0 13 317.0 14 331.0 15 341.0 16 365.5 17 389.0 18 417.0 19 460.5 20 584.0 21 703.5 22 803.5 23 994.0 24 1165.5 25 1500.5 26 2281.0 27 3783.0 28 5357.0 29 8293.0 30 14612.0 31 21364.0 32 35731.5 33 49798.0 34 59046.0 35 67358.5 36 74709.0 37 76440.0 38 63675.5 39 53490.5 40 44800.5 41 34862.0 42 28221.5 43 25457.5 44 25456.5 45 23788.0 46 20748.0 47 30581.0 48 57064.5 49 51911.0 50 23504.5 51 10190.5 52 4467.0 53 2640.0 54 2121.0 55 1725.0 56 1376.5 57 1117.5 58 1160.0 59 1090.5 60 805.0 61 647.5 62 515.5 63 351.5 64 207.0 65 138.5 66 107.5 67 112.0 68 85.0 69 33.5 70 25.5 71 31.0 72 35.5 73 49.5 74 48.5 75 25.0 76 22.0 77 26.0 78 22.5 79 12.5 80 3.5 81 3.5 82 3.0 83 2.0 84 1.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046979823067411275 2 0.0018028374540541574 3 4.2419704801274294E-4 4 0.009332335056280344 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0020679606090621217 24-25 0.0 26-27 0.0 28-29 0.020520532197616438 30-31 0.017020906551511308 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.036109773712084736 50-51 0.012619862178379102 52-53 0.0 54-55 4.7722167901433576E-4 56-57 0.0 58-59 0.0 60-61 5.302463100159287E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.010923073986328129 82-83 0.004878266052146543 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 942958.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.179201290143265 #Duplication Level Percentage of deduplicated Percentage of total 1 72.29890641955274 10.251407471800693 2 10.411366303689297 2.952497170508508 3 4.143905624600456 1.7627181593570014 4 2.242525727030757 1.2718889472757589 5 1.4160879614884492 1.0039498125246682 6 0.9963443289826035 0.8476420076962233 7 0.8169546754152454 0.8108635351345493 8 0.6442070415190907 0.7307473051381493 9 0.5138168568637289 0.6556961375765588 >10 4.5345277935546635 13.832010496765742 >50 0.8829470407905261 8.821856410126887 >100 0.9326693144186614 26.643961192660882 >500 0.10773159286096008 10.409544874420838 >1k 0.054242480321602275 14.56757309703014 >5k 0.0022601033467334283 1.9467156410746793 >10k+ 0.0015067355644889523 3.4909277409087203 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22089 2.3425221483883694 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10591 1.12316773387574 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7435 0.7884762629936858 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5635 0.5975875913879515 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5154 0.5465778963644192 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4798 0.5088243590912851 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 4735 0.5021432555850844 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4731 0.5017190585370717 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4570 0.4846451273545587 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4321 0.45823886111576545 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3884 0.41189533362037334 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 3590 0.38071685059143673 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3444 0.36523365833897165 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 3289 0.3487960227284778 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 3267 0.3464629389644077 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3022 0.3204808697736272 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 2907 0.30828520464326087 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2817 0.2987407710629742 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2675 0.2836817758585218 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2577 0.2732889481822096 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 2570 0.27254660334818726 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2203 0.23362652419301813 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2077 0.22026431718061676 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 2064 0.21888567677457532 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1922 0.20382668157012296 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 1845 0.19566088839587767 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 1840 0.19513064208586173 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1838 0.19491854356185537 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1827 0.19375200167982032 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 1822 0.19322175536980438 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1819 0.19290360758379482 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1810 0.19194916422576616 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 1771 0.1878132430076419 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1702 0.18049584392942208 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1679 0.17805671090334882 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1673 0.1774204153313297 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1637 0.17360264189921504 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1589 0.16851227732306212 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1556 0.165012651676957 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 1549 0.16427030684293467 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 1541 0.1634219127469092 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 1509 0.16002833636280725 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1507 0.1598162378388009 No Hit GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 1445 0.15324118359460334 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1402 0.1486810653284664 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1387 0.1470903263984186 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1345 0.1426362573942848 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 1321 0.14009107510620833 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 1320 0.13998502584420514 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 1293 0.13712169577011912 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 1283 0.1360612031500873 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1265 0.13415231643402994 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 1261 0.1337281193860172 No Hit TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA 1259 0.1335160208620108 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 1254 0.1329857745519949 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1247 0.1322434297179726 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 1243 0.13181923266995985 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1192 0.12641072030779738 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 1192 0.12641072030779738 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1179 0.12503207990175597 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1176 0.12471393211574641 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 1166 0.12365343949571454 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1150 0.12195665130366358 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 1147 0.12163850351765403 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1130 0.11983566606359987 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1126 0.11941146901558714 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1110 0.11771468082353614 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 1102 0.11686628672751065 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 1073 0.11379085812941826 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 1069 0.11336666108140553 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 1065 0.1129424640333928 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1064 0.1128364147713896 No Hit ACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTAT 1051 0.11145777436534819 No Hit GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG 1036 0.10986703543530041 No Hit GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG 1018 0.10795814871924306 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1014 0.10753395167123032 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 1011 0.10721580388522076 No Hit ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC 989 0.10488272012115067 No Hit TATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGC 970 0.10286778414309015 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 952 0.10095889742703282 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0604926200318572E-4 0.0 10-11 0.0 0.0 0.0 3.7117241701115E-4 0.0 12-13 0.0 0.0 0.0 7.423448340223E-4 0.0 14-15 0.0 0.0 0.0 0.0012725911440382286 0.0 16-17 0.0 0.0 0.0 0.0032345024910971644 0.0 18-19 0.0 0.0 0.0 0.004613142897138579 0.0 20-21 0.0 0.0 0.0 0.008112768543243706 0.0 22-23 0.0 0.0 0.0 0.015801340038474672 0.0 24-25 0.0 0.0 0.0 0.024709478046742273 0.0 26-27 0.0 0.0 0.0 0.03223897564896846 0.0 28-29 0.0 0.0 0.0 0.04374532057631411 0.0 30-31 0.0 0.0 0.0 0.06495517297695125 0.0 32-33 0.0 0.0 0.0 0.1114047497343466 0.0 34-35 0.0 0.0 0.0 0.176731095128309 0.0 36-37 0.0 0.0 0.0 0.2567452633097126 0.0 38-39 0.0 0.0 0.0 0.3859662890605944 0.0 40-41 0.0 0.0 0.0 0.5410633347402536 0.0 42-43 0.0 0.0 0.0 0.6782910797723759 0.0 44-45 0.0 0.0 0.0 0.8382133668731799 0.0 46-47 0.0 0.0 0.0 1.050364915510553 0.0 48-49 0.0 0.0 0.0 1.2430033999393397 0.0 50-51 0.0 0.0 0.0 1.443542554387364 0.0 52-53 0.0 0.0 0.0 1.643180290108361 0.0 54-55 0.0 0.0 0.0 1.8396365479692627 0.0 56-57 0.0 0.0 0.0 2.1004116832350963 0.0 58-59 0.0 0.0 0.0 2.359490030308879 0.0 60-61 0.0 0.0 0.0 2.637233047495222 0.0 62-63 0.0 0.0 0.0 2.900394291156128 0.0 64-65 0.0 0.0 0.0 3.1653053476400856 0.0 66-67 0.0 0.0 0.0 3.4878541780227748 0.0 68-69 0.0 0.0 0.0 3.8528757378377403 0.0 70-71 0.0 0.0 0.0 4.263074283266063 0.0 72-73 0.0 0.0 0.0 4.6346708973252255 0.0 74-75 0.0 0.0 0.0 5.020584161754819 0.0 76-77 0.0 0.0 0.0 5.411322667605557 0.0 78-79 0.0 0.0 0.0 5.96569518472721 0.0 80-81 0.0 0.0 0.0 6.55092803709179 0.0 82-83 0.0 0.0 0.0 7.082499962882759 0.0 84-85 0.0 0.0 0.0 7.590422903247017 0.0 86-87 0.0 0.0 0.0 8.101898493888381 0.0 88-89 0.0 0.0 0.0 8.677162715624661 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 175 0.0 43.424137 3 ATGTATT 135 0.0 42.24703 1 TGGGTAT 205 0.0 39.38622 6 GAGTTAA 75 6.925815E-6 37.996117 9 GACATAT 190 0.0 37.522034 1 CATGGGG 590 0.0 37.030113 4 GATGACC 315 0.0 36.186783 9 GGGGAGC 105 4.660251E-8 36.18678 7 TAACTTT 1170 0.0 35.722847 6 TGGACAG 55 0.0025806841 34.541924 5 GGACAGA 55 0.0025806841 34.541924 6 TACAATC 335 0.0 34.026375 7 GCTGTAC 70 2.0459804E-4 33.948505 1 ACTTTAT 1275 0.0 33.8985 8 ATAGACC 85 1.6330341E-5 33.52599 3 CTATACA 370 0.0 33.37497 4 ATACTCA 290 0.0 32.755276 6 TCTAGAC 235 0.0 32.33712 3 GGATATG 265 0.0 32.283108 1 ACTCAAT 280 0.0 32.22885 8 >>END_MODULE