FastQCFastQC Report
Fri 10 Feb 2017
SRR2031614_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031614_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467123
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19650.028.5929991
GTATCAA29250.025.3944591
TCAACGC28300.024.6938044
ATCAACG29100.024.3400063
CAACGCA29450.023.7295285
AACGCAG30350.023.3303626
CGCAGAG32500.021.2020848
ACGCAGA33200.021.1844677
TATCAAC34200.021.0098842
GTACATG36400.020.5370711
CGTATAC1404.8275245E-420.3727953
ACATGGG33250.020.3013113
ACGGATC1200.004751417319.8007988
CCTACAC6600.019.4467583
TACATGG38200.019.308172
TCTATAC8950.019.1208363
CATGGGG16350.018.8996034
TCTACAC9600.018.8165423
CAGTGCG1801.2292607E-418.4807439
ACAGCGT2603.265759E-718.2776578
Produced by FastQC (version 0.11.5)