FastQCFastQC Report
Fri 10 Feb 2017
SRR2031614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467123
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC30050.027.502734
GTATCAA31600.027.2225631
ATCAACG30500.027.096953
CAACGCA30650.026.9625035
AACGCAG31400.026.3184936
GGTATCA22250.025.2051661
ACGCAGA34200.024.4415077
TATACGG1751.4884608E-724.4256365
CGCAGAG34000.024.3059028
TATCAAC34500.024.0929492
GTACATG31650.022.6746581
CTGATCG1050.002198043522.616339
ACATGGG28500.021.9988563
GCAGAGT40900.020.0892739
TACATGG34800.019.3811762
GTATATA16400.018.5469991
CATGGGG14650.018.4802684
CGTACGA650.00755623318.27326222-23
GTATAGA8900.017.6222551
GTACTAA7600.017.5098321
Produced by FastQC (version 0.11.5)