FastQCFastQC Report
Fri 10 Feb 2017
SRR2031613_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031613_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905353
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40160.21077459137493157No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21270.11163285753348591No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGG42900.023.2720223
GTACATG50000.023.0506841
GTATTGC5400.022.0487861
TACACCG1456.1120844E-419.6723125
TACCGTC1456.130521E-419.6635157
TACATGG55700.018.9652522
CGCCGTA6800.018.86135394-95
TAATACT22950.018.8508454
TAAGGCG1300.007526974718.285165
GTATAAA15350.017.9952581
ATACAGC11250.017.7417936
GTATAGA9700.017.67541
GTCCCAC7550.017.6252259
GTCGGAC951.893489E-417.46791624-25
GTATATA16200.017.3450451
CATGGGA21750.017.0493354
CATGGGG21600.016.9476344
GTATAGT8150.016.9464531
CATGGGC12650.016.9119664
GTATACA14950.016.8839131
Produced by FastQC (version 0.11.5)