##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031613_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1905353 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09043206167046 34.0 31.0 34.0 30.0 34.0 2 32.322436314950565 34.0 31.0 34.0 31.0 34.0 3 32.47288980047267 34.0 31.0 34.0 31.0 34.0 4 35.550284382998846 37.0 35.0 37.0 33.0 37.0 5 35.290367716638336 37.0 35.0 37.0 33.0 37.0 6 35.50273571353969 37.0 35.0 37.0 33.0 37.0 7 35.649920513416674 37.0 36.0 37.0 33.0 37.0 8 35.72245038058564 37.0 36.0 37.0 35.0 37.0 9 37.670738703011985 39.0 38.0 39.0 35.0 39.0 10-11 37.62865306323815 39.0 38.0 39.0 35.0 39.0 12-13 37.66476553163639 39.0 38.0 39.0 35.0 39.0 14-15 39.13149977983082 41.0 39.0 41.0 36.0 41.0 16-17 39.11703028257756 41.0 39.0 41.0 36.0 41.0 18-19 39.052724350815836 41.0 39.0 41.0 36.0 41.0 20-21 39.07047355529395 41.0 39.0 41.0 36.0 41.0 22-23 39.01778856726286 41.0 39.0 41.0 36.0 41.0 24-25 38.96346293836365 41.0 39.0 41.0 36.0 41.0 26-27 38.89348194271612 41.0 39.0 41.0 35.0 41.0 28-29 38.81009844370046 41.0 39.0 41.0 35.0 41.0 30-31 38.72364910859038 40.0 39.0 41.0 35.0 41.0 32-33 38.62075557652571 40.0 38.0 41.0 34.5 41.0 34-35 38.527635823912945 40.0 38.0 41.0 34.0 41.0 36-37 38.433154381366606 40.0 38.0 41.0 34.0 41.0 38-39 38.29711528519912 40.0 38.0 41.0 34.0 41.0 40-41 38.2000379982082 40.0 38.0 41.0 34.0 41.0 42-43 38.08584288580646 40.0 38.0 41.0 33.0 41.0 44-45 37.940387424272565 40.0 38.0 41.0 33.0 41.0 46-47 37.81314958435523 40.0 38.0 41.0 33.0 41.0 48-49 37.972304869491374 40.0 38.0 41.0 33.0 41.0 50-51 37.76188795462048 39.5 37.5 40.5 33.0 41.0 52-53 37.92605149806886 40.0 38.0 41.0 33.0 41.0 54-55 38.25083016113025 40.0 38.0 41.0 34.0 41.0 56-57 38.19903083575589 40.0 38.0 41.0 33.5 41.0 58-59 38.07527975131117 40.0 38.0 41.0 33.0 41.0 60-61 37.86939742924277 40.0 37.5 41.0 33.0 41.0 62-63 37.63652168390844 40.0 37.0 41.0 32.5 41.0 64-65 37.43438669894765 40.0 37.0 41.0 32.0 41.0 66-67 37.281633639540814 39.5 36.0 41.0 32.0 41.0 68-69 37.08704581250824 39.0 36.0 41.0 31.5 41.0 70-71 36.80994965237413 39.0 35.5 41.0 31.0 41.0 72-73 36.46570189355988 38.5 35.0 40.5 31.0 41.0 74-75 36.03167103418632 37.5 35.0 40.0 30.5 41.0 76-77 35.59232357468669 37.0 35.0 39.0 30.0 41.0 78-79 35.159682221614574 36.5 35.0 39.0 30.0 40.5 80-81 34.720127189030066 36.0 34.5 38.5 29.0 39.0 82-83 34.29304543567517 35.5 34.0 37.0 29.0 39.0 84-85 33.90887069220244 35.0 34.0 37.0 29.0 39.0 86-87 33.569836665436796 35.0 34.0 36.5 29.0 37.0 88-89 33.22508217637362 35.0 34.0 36.0 28.0 37.0 90-91 32.94123949735298 35.0 34.0 36.0 27.0 37.0 92-93 32.692650128348916 35.0 34.0 35.0 26.0 36.0 94-95 32.47100038680496 35.0 33.5 35.0 26.0 36.0 96-97 32.318276455858836 35.0 33.0 35.0 25.0 36.0 98-99 32.17022252569471 35.0 33.0 35.0 25.0 35.5 100-101 31.67867319074208 34.5 32.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4262328829099147 1101 2 0.42356676674157256 1101 3 0.372567744614841 1101 4 0.4296126700844667 1101 5 0.44013423731789914 1101 6 0.43833103612729474 1101 7 0.3795549582645279 1101 8 0.370422411675122 1101 9 0.38142916737857036 1101 10-11 0.4290350661985016 1101 12-13 0.39489252341049763 1101 14-15 0.4105148375643992 1101 16-17 0.4962562029455668 1101 18-19 0.46466873547410614 1101 20-21 0.19903460430379738 1101 22-23 0.36059332793261234 1101 24-25 0.3100935292815521 1101 26-27 0.28851160409277554 1101 28-29 0.2897668783219771 1101 30-31 0.2400187087907213 1101 32-33 0.5123018484908926 1101 34-35 0.45857646815981923 1101 36-37 0.4119108193550147 1101 38-39 0.3400471125073068 1101 40-41 0.2682755333343323 1101 42-43 0.19659313325571048 1101 44-45 0.38832597858360174 1101 46-47 0.44910744571269134 1101 48-49 0.3891125317446793 1101 50-51 0.21449466227725367 1101 52-53 0.1961326949139064 1101 54-55 0.15131742710539697 1101 56-57 0.19318684048477053 1101 58-59 0.2519455743868093 1101 60-61 0.16809571112325727 1101 62-63 0.07195455569210196 1101 64-65 0.202273954381063 1101 66-67 0.21615557174497724 1101 68-69 0.10962996772902756 1101 70-71 0.22776052158913984 1101 72-73 0.3091236563882589 1101 74-75 0.13875435458076169 1101 76-77 0.08717186305972291 1101 78-79 0.16197149715670633 1101 80-81 0.08593464831675846 1101 82-83 0.21566756934196718 1101 84-85 0.12977359621261542 1101 86-87 0.1794508234798755 1101 88-89 0.22164362876784338 1101 90-91 0.02068414069461255 1101 92-93 -0.04949637667886719 1101 94-95 0.08933048553579681 1101 96-97 0.10211739496756422 1101 98-99 0.04163399553156921 1101 100-101 0.09033411033206917 1102 1 -0.015601860095941333 1102 2 0.03239348545768905 1102 3 0.11273256786359553 1102 4 0.17652223417034918 1102 5 0.1358184706729233 1102 6 0.1626858398575024 1102 7 0.15530859088217852 1102 8 0.32511770553762886 1102 9 0.3503946846199497 1102 10-11 0.23574727613127067 1102 12-13 0.3240835882554265 1102 14-15 0.26581737497494373 1102 16-17 0.27362553714639404 1102 18-19 0.49534537828477454 1102 20-21 0.36960497949355897 1102 22-23 0.23749638583762334 1102 24-25 0.36988533149365566 1102 26-27 0.3372320551879824 1102 28-29 0.3113457115468776 1102 30-31 0.2633867417553759 1102 32-33 0.2664441169792937 1102 34-35 0.1779323562535069 1102 36-37 0.215185597060497 1102 38-39 0.08963505438543251 1102 40-41 0.36253925053232905 1102 42-43 0.23536997125180648 1102 44-45 -0.19982192859489345 1102 46-47 -0.5506323070524388 1102 48-49 -0.05689701065393393 1102 50-51 -0.10196597532846852 1102 52-53 0.045904978583386935 1102 54-55 -4.0671082564358585E-4 1102 56-57 0.1941085494416086 1102 58-59 0.1316462900667048 1102 60-61 -0.07654753329227049 1102 62-63 -0.28295761086612714 1102 64-65 0.06592609057711485 1102 66-67 0.07822453726222278 1102 68-69 0.06959526809771432 1102 70-71 0.04989238018814035 1102 72-73 0.0720058945144828 1102 74-75 0.05082332009801149 1102 76-77 0.11718704414842307 1102 78-79 0.11341370058329403 1102 80-81 -0.012796646413235635 1102 82-83 -0.09507847273133763 1102 84-85 -0.011074376027679023 1102 86-87 -0.030004826053847466 1102 88-89 -0.20479315284134714 1102 90-91 -0.11409139606531227 1102 92-93 -0.09485530099028949 1102 94-95 -0.04880657800791255 1102 96-97 -0.11721680585655037 1102 98-99 -0.16043713871263066 1102 100-101 -0.07929286863127771 1103 1 -0.044419493495787776 1103 2 -0.08220611081306828 1103 3 -0.14050718429798792 1103 4 -0.030569865105043448 1103 5 -0.010490207172146881 1103 6 0.03580283965914077 1103 7 0.11341876462577716 1103 8 0.0605221932332185 1103 9 0.2771878375586567 1103 10-11 0.21998024890871903 1103 12-13 0.2237872089833033 1103 14-15 0.3128670010003134 1103 16-17 0.2233408263495491 1103 18-19 0.24659740568235833 1103 20-21 0.07250809614330933 1103 22-23 0.05537015627204056 1103 24-25 0.18637872130241107 1103 26-27 -0.0013246711045979964 1103 28-29 0.15488312651285696 1103 30-31 0.26226948524728044 1103 32-33 0.20434624180864347 1103 34-35 0.16615361388176808 1103 36-37 0.2028006773979527 1103 38-39 0.0850569281091893 1103 40-41 0.10227215913317167 1103 42-43 -0.014863981677564198 1103 44-45 0.023657960787964782 1103 46-47 0.10246158916813641 1103 48-49 -0.008804806362270767 1103 50-51 0.0699246677212031 1103 52-53 0.07453591637874979 1103 54-55 -0.10661784021736054 1103 56-57 -0.03100259278542694 1103 58-59 0.011626687028240212 1103 60-61 0.044402630649805985 1103 62-63 0.058917046372407356 1103 64-65 0.06450487028796914 1103 66-67 -0.24765264036697943 1103 68-69 -0.1557334911933097 1103 70-71 -0.0905709749652388 1103 72-73 -0.021378663425331013 1103 74-75 0.016266547131529308 1103 76-77 -0.6572718303104494 1103 78-79 -0.27339158756970505 1103 80-81 -0.05036038545945587 1103 82-83 -0.19428559983602867 1103 84-85 -0.16209420076717862 1103 86-87 -0.06942326924586695 1103 88-89 -0.08928924973636043 1103 90-91 -0.04049307165836069 1103 92-93 -0.062024295517780104 1103 94-95 -0.22853668617467093 1103 96-97 -0.08763313705455289 1103 98-99 -0.056263908528613626 1103 100-101 0.012131385461437105 1104 1 0.164464059925578 1104 2 0.1842034833276216 1104 3 0.2049522115815705 1104 4 0.044357075368118615 1104 5 0.14895538924824336 1104 6 0.22056765467606 1104 7 0.18093668088318537 1104 8 0.28508474552686636 1104 9 0.3196533085955551 1104 10-11 0.2500831991797341 1104 12-13 0.1833589164541749 1104 14-15 0.18913265877857555 1104 16-17 0.20607937631499595 1104 18-19 0.425893366490925 1104 20-21 0.22227709465180112 1104 22-23 0.3503608798341915 1104 24-25 0.2789672718741514 1104 26-27 0.3199129148388735 1104 28-29 0.32501178403647657 1104 30-31 0.29619820825163146 1104 32-33 0.19304475151555067 1104 34-35 0.2780226039020164 1104 36-37 0.2381724018453042 1104 38-39 0.2897084857019223 1104 40-41 0.24691183318460475 1104 42-43 0.17941979243813932 1104 44-45 0.20166855896663094 1104 46-47 0.5076721582442545 1104 48-49 0.5045156733447627 1104 50-51 0.4315299158810788 1104 52-53 0.40585632332940946 1104 54-55 0.42908128791874134 1104 56-57 0.47091938812815215 1104 58-59 0.3680480434495905 1104 60-61 0.4791644141510858 1104 62-63 0.48882234182392637 1104 64-65 0.49893318706922685 1104 66-67 0.4264291135467033 1104 68-69 0.4155677565785325 1104 70-71 0.3675456050000747 1104 72-73 0.3318535385463619 1104 74-75 0.23328668852142442 1104 76-77 0.2026297060616713 1104 78-79 0.20911656720735294 1104 80-81 0.23111584139492436 1104 82-83 0.13107484737314934 1104 84-85 0.25834063983572264 1104 86-87 0.3034652801132651 1104 88-89 0.23667143756886588 1104 90-91 0.08051896952174786 1104 92-93 0.19062828066420678 1104 94-95 0.07178950490781943 1104 96-97 0.16917261554952034 1104 98-99 0.20860567566870003 1104 100-101 0.2196325677525337 1105 1 0.2002661200899496 1105 2 0.26007200719197954 1105 3 0.2153150802474073 1105 4 0.2276938265341215 1105 5 -0.014056299739500844 1105 6 0.10329918654866077 1105 7 0.2010146074681387 1105 8 0.2353253083650344 1105 9 0.14723889901300424 1105 10-11 0.24149392276120096 1105 12-13 0.12635998386961234 1105 14-15 0.17888021239910756 1105 16-17 0.2075905841770549 1105 18-19 0.19204210005246125 1105 20-21 0.14766475575002858 1105 22-23 0.20174194714446259 1105 24-25 0.1587705098762413 1105 26-27 0.18828954948307342 1105 28-29 0.12432506257931664 1105 30-31 0.1612411414882189 1105 32-33 0.10076537261462448 1105 34-35 0.10893085349844966 1105 36-37 0.21368284200957532 1105 38-39 0.20447615195318747 1105 40-41 0.28428188981546754 1105 42-43 0.27906118291763704 1105 44-45 0.2856152618097454 1105 46-47 0.4573879523119544 1105 48-49 0.2760940284733593 1105 50-51 0.15815535822749638 1105 52-53 0.2038445344357882 1105 54-55 0.26825806348281844 1105 56-57 0.4314297494193511 1105 58-59 0.3976895916752099 1105 60-61 0.33483722765836177 1105 62-63 0.28186678942329024 1105 64-65 0.3345964306450142 1105 66-67 0.19733178944677832 1105 68-69 0.1522579274175726 1105 70-71 0.17208028022709954 1105 72-73 0.18625702158076507 1105 74-75 0.16132869105587844 1105 76-77 0.19535813559384962 1105 78-79 0.2019259730828722 1105 80-81 0.2755403046637426 1105 82-83 0.10491317862949501 1105 84-85 0.09095990905052531 1105 86-87 0.1541717198358441 1105 88-89 0.03069969515775739 1105 90-91 0.2036271991370029 1105 92-93 0.1441857186568285 1105 94-95 0.12806490860644004 1105 96-97 0.2683150024586922 1105 98-99 0.12295741797463222 1105 100-101 -0.0024881711303095244 1106 1 0.2404444520824427 1106 2 0.22108702462292484 1106 3 0.14522256214603857 1106 4 0.22192317756657332 1106 5 0.2125694712446773 1106 6 0.05961622865110172 1106 7 0.20936805343220755 1106 8 0.24463242823630793 1106 9 0.31195128839353714 1106 10-11 0.29129866263784976 1106 12-13 0.2693809010055688 1106 14-15 0.3856715773763142 1106 16-17 0.3813746080440481 1106 18-19 0.48729970933109 1106 20-21 0.31860619447355276 1106 22-23 0.4037699707432907 1106 24-25 0.3810988820940011 1106 26-27 0.24099134621142326 1106 28-29 0.3855881357892983 1106 30-31 0.3667511642825332 1106 32-33 0.3800786938803782 1106 34-35 0.3320704351557282 1106 36-37 0.3045902568661063 1106 38-39 0.2360580103602672 1106 40-41 0.3015686959297099 1106 42-43 0.25603892493962377 1106 44-45 0.41122366888938444 1106 46-47 0.6990621612151457 1106 48-49 0.48370833525801515 1106 50-51 0.21339637458576988 1106 52-53 0.2241532633175254 1106 54-55 0.29717583634417366 1106 56-57 0.34954201141988506 1106 58-59 0.3291591545606991 1106 60-61 0.46709704932636953 1106 62-63 0.4982066258405595 1106 64-65 0.691195887603989 1106 66-67 0.4297843571592992 1106 68-69 0.40477787244305574 1106 70-71 0.37814423839282796 1106 72-73 0.3103796080650838 1106 74-75 0.4129695465131036 1106 76-77 0.4392514380878225 1106 78-79 0.36361560166962903 1106 80-81 0.4609008235696095 1106 82-83 0.3780686827861075 1106 84-85 0.24630484066757674 1106 86-87 0.21129913335153105 1106 88-89 0.34202645836506207 1106 90-91 0.35715109964652214 1106 92-93 0.3376239728804862 1106 94-95 0.24455391370163682 1106 96-97 0.4065574937432217 1106 98-99 0.41031579153322895 1106 100-101 0.3028433520662617 1107 1 0.1621443275494414 1107 2 0.12679686273002488 1107 3 0.09765718232038978 1107 4 0.17875536934739245 1107 5 0.17318902292586813 1107 6 0.12315452254898673 1107 7 0.1261675553001993 1107 8 0.263139588883881 1107 9 0.3350677267496991 1107 10-11 0.3764597772082041 1107 12-13 0.26413183500307724 1107 14-15 0.30677369443733227 1107 16-17 0.247437030210925 1107 18-19 0.2976896532912363 1107 20-21 0.17024769509621507 1107 22-23 0.2059275049898588 1107 24-25 0.3086860551948689 1107 26-27 0.288021234131719 1107 28-29 0.2789256202226724 1107 30-31 0.2888549936723237 1107 32-33 0.3405916141792531 1107 34-35 0.3856165123661981 1107 36-37 0.3246385134040821 1107 38-39 0.5007326678945248 1107 40-41 0.3946104144851219 1107 42-43 0.34068400588607517 1107 44-45 0.28651476477979543 1107 46-47 0.4794116319498869 1107 48-49 0.4099583352580183 1107 50-51 0.21867501717605364 1107 52-53 0.42959068548439205 1107 54-55 0.4793761788099289 1107 56-57 0.5096517561272336 1107 58-59 0.4350993787201105 1107 60-61 0.4934972672591158 1107 62-63 0.5679030766500262 1107 64-65 0.4889690818754744 1107 66-67 0.49170605703476866 1107 68-69 0.559584067959868 1107 70-71 0.5445981611823711 1107 72-73 0.3659345582518867 1107 74-75 0.5376442040473464 1107 76-77 0.4986482313630347 1107 78-79 0.4940615854803312 1107 80-81 0.6212767575671236 1107 82-83 0.5969720140438923 1107 84-85 0.5971594483886236 1107 86-87 0.4375078817948648 1107 88-89 0.38186145213840916 1107 90-91 0.47206781820193555 1107 92-93 0.5170090911868357 1107 94-95 0.43493685268047244 1107 96-97 0.4272813418129573 1107 98-99 0.18432808916710286 1107 100-101 0.39300057498033425 1108 1 0.0880662395776497 1108 2 0.06940885047639966 1108 3 0.023137790377724343 1108 4 -0.05563483931106106 1108 5 -0.0220153005236412 1108 6 0.04669189033119636 1108 7 0.024855203413025606 1108 8 0.06563597637593688 1108 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-0.823071876478771 2316 62-63 -0.7601938022067287 2316 64-65 -1.0510776040389018 2316 66-67 -1.4310709730486053 2316 68-69 -1.791689463472153 2316 70-71 -1.1326435724510375 2316 72-73 -0.8654318982537106 2316 74-75 -0.9455073185500851 2316 76-77 -0.7781927628905763 2316 78-79 -0.9913795573193696 2316 80-81 -1.0178789868665845 2316 82-83 -1.0735352205975062 2316 84-85 -1.6360050859528883 2316 86-87 -1.1258556261266577 2316 88-89 -0.9660401947128037 2316 90-91 -1.1077299133987282 2316 92-93 -1.33887674053166 2316 94-95 -1.1464519974275902 2316 96-97 -1.0886855759673395 2316 98-99 -0.7424346670888688 2316 100-101 -1.2230866316271758 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 11.0 7 155.0 8 914.0 9 1071.0 10 1022.0 11 1139.0 12 1456.0 13 1866.0 14 2100.0 15 2232.0 16 2656.0 17 3163.0 18 3872.0 19 4569.0 20 5218.0 21 5793.0 22 6202.0 23 7027.0 24 8085.0 25 9257.0 26 11034.0 27 13008.0 28 15838.0 29 19360.0 30 24274.0 31 29555.0 32 37572.0 33 49125.0 34 68577.0 35 102171.0 36 169632.0 37 328350.0 38 635228.0 39 333711.0 40 110.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.66155965354158 21.56299704253102 15.614624072363064 22.160819231564336 2 13.133454355043138 21.288565134158148 44.01195396679734 21.566026544001364 3 17.33711206075975 27.73452003184817 31.90402214647004 23.024345760922042 4 11.405420987679 17.97365782527136 42.28025304816143 28.340668138888216 5 11.348599919812012 39.74119905247683 37.43221641917482 11.477984608536337 6 27.370998506975248 41.17571886328174 19.11786901876352 12.33541361097949 7 24.175702827611556 34.16565398212452 25.030091323024372 16.628551867239555 8 22.654384868926147 39.362278327117764 21.56928940576628 16.41404739818981 9 23.729223533717438 18.66215568868573 22.661827566569613 34.94679321102721 10-11 22.468019825906683 28.317261839179174 30.84375278570082 18.370965549213324 12-13 23.076114075013205 25.55568672622633 31.19928000448025 20.16891919428021 14-15 20.29326429284778 27.916195326514483 28.29132160886209 23.499218771775652 16-17 19.829324006764846 31.85927359612285 28.608597466309078 19.702804930803232 18-19 19.910491754562173 31.146223227725716 29.758708913914468 19.184576103797642 20-21 20.708768348031 29.695213077929356 29.781234131831596 19.81478444220805 22-23 20.425873840700383 29.465353664124184 29.66657621973461 20.442196275440825 24-25 20.569154377167905 29.883255228821113 29.17795285178127 20.369637542229707 26-27 20.28185853225098 29.83205736679765 29.67830632958827 20.2077777713631 28-29 20.16214318291676 29.804739594185435 29.68135038494179 20.351766837956013 30-31 20.55529867693808 29.65607947713626 29.505949816123312 20.282672029802352 32-33 20.139286525908847 29.80463462675945 29.885223368058305 20.170855479273396 34-35 20.42343334804627 29.881103396588454 29.429244869585848 20.266218385779432 36-37 20.63441787427317 29.39549784213214 29.46860765432967 20.50147662926502 38-39 20.376329215636158 29.38090736992043 29.743333649985072 20.499429764458345 40-41 20.08281405072971 29.29192123454289 30.19918093917505 20.42608377555235 42-43 20.07113642458904 29.521537478881864 29.62624248630044 20.781083610228656 44-45 20.198087709916344 29.58094910239043 29.391559592524803 20.829403595168426 46-47 20.276759448374445 29.548513896481982 29.48177354720749 20.69295310793608 48-49 20.32761179328912 29.098772079585 29.868619082014575 20.704997045111302 50-51 19.986970276896056 29.330899297728912 29.922882525571026 20.759247899804002 52-53 20.14734000940669 29.39583663237242 29.691325490220795 20.765497868000097 54-55 19.868919710367976 29.595308318385122 29.526209492093425 21.00956247915348 56-57 20.261673669011522 29.1515690646983 29.597429974799965 20.989327291490206 58-59 20.537678440985207 28.745563190179535 29.463648038084923 21.25311033075033 60-61 20.169312475199654 29.028614675499753 29.918431487968384 20.883641361332216 62-63 20.12746868983557 29.115145068039155 29.587372134762536 21.17001410736274 64-65 20.118134847362516 29.176768968042527 29.29785206263002 21.40724412196494 66-67 20.212329429269758 28.72762589081372 29.633019425045553 21.427025254870973 68-69 20.087933936057695 28.469216029238453 29.74587638913919 21.69697364556466 70-71 20.312332557978277 28.732578150116307 29.370775207969558 21.584314083935862 72-73 20.15877196875613 28.77940864807998 29.393692137638787 21.668127245525103 74-75 19.96093830070045 28.6992327992133 29.266368518120434 22.073460381965816 76-77 19.91654244726768 28.359841069300224 29.78430533040607 21.939311153026026 78-79 20.14780644886637 28.019158129197013 29.797323895472257 22.03571152646436 80-81 20.265088689015222 28.347890492343208 29.54377062592501 21.843250192716564 82-83 20.17335899669157 28.285578754605694 29.466931985446692 22.074130263256045 84-85 20.360226463487322 28.118542124882744 29.678978839118514 21.842252572511423 86-87 20.083086561501073 28.0820392474886 29.94787590642034 21.886998284589986 88-89 20.240877394504174 27.775815018601264 29.781620641350315 22.201686945544246 90-91 20.660592273079207 27.85788430663138 29.28051775881307 22.201005661476344 92-93 20.795869798016277 27.61719035445065 29.60511685784175 21.98182298969132 94-95 20.57955850043922 27.29412624115749 29.616217694825625 22.510097563577666 96-97 20.425658437851553 27.178387652338404 30.061979428093537 22.333974481716503 98-99 20.659951975892877 27.18386121069809 29.533066275946634 22.623120537462402 100-101 20.797545738404136 27.177381215907747 29.318111575631008 22.70696147005711 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11.0 1 10.0 2 19.0 3 30.0 4 25.0 5 26.0 6 42.0 7 69.5 8 117.5 9 144.0 10 155.0 11 165.5 12 183.5 13 217.0 14 249.5 15 289.0 16 325.0 17 365.5 18 424.0 19 479.5 20 542.5 21 619.0 22 703.5 23 817.0 24 908.0 25 1086.5 26 1374.5 27 1751.0 28 2737.0 29 5336.0 30 10639.5 31 19594.5 32 33935.0 33 53826.5 34 75864.5 35 99330.5 36 124521.5 37 142383.5 38 146150.0 39 140355.5 40 130896.5 41 116733.5 42 99236.5 43 83266.0 44 69321.0 45 57983.5 46 49097.5 47 44020.5 48 43555.0 49 38839.0 50 30785.5 51 26859.5 52 25187.0 53 25010.5 54 24848.0 55 24047.0 56 22876.5 57 21494.5 58 19925.5 59 17559.0 60 15032.0 61 12790.5 62 10204.5 63 7680.0 64 5763.5 65 4264.0 66 3043.0 67 2222.0 68 1696.0 69 1282.0 70 1012.5 71 764.5 72 548.0 73 422.5 74 334.5 75 268.0 76 191.5 77 128.0 78 89.5 79 62.0 80 44.5 81 27.0 82 20.0 83 20.5 84 15.5 85 14.0 86 13.0 87 8.0 88 7.0 89 7.5 90 6.0 91 3.5 92 2.0 93 1.5 94 1.0 95 1.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47954368560576444 2 0.3711123345647762 3 0.2010651044714549 4 0.22940630948700844 5 0.25291901290731955 6 0.20174739274034784 7 0.19114568271601115 8 0.19298261267072295 9 0.19009600845617583 10-11 0.20476520623737438 12-13 0.19285140338824353 14-15 0.2025871321482161 16-17 0.08856626567360484 18-19 0.037998208206038456 20-21 2.8866042145471205E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.139632918414593 46-47 0.20754684302593798 48-49 0.09069185604977135 50-51 0.06534222267474846 52-53 0.07292611920205862 54-55 0.08176962484117116 56-57 0.11446697803504129 58-59 0.0953891483625344 60-61 0.13950170913211357 62-63 0.23998177765484924 64-65 0.13265258458668813 66-67 0.106909323364227 68-69 0.11053069956065883 70-71 0.0896946655029278 72-73 0.07492050029574572 74-75 0.14286066676358658 76-77 0.2176499577768529 78-79 0.13971164398408065 80-81 0.08662436829290951 82-83 0.09084930718874665 84-85 0.10032261738376039 86-87 0.07531412814318397 88-89 0.09727856203023796 90-91 0.11690747068915838 92-93 0.1663733701838977 94-95 0.1334398402815646 96-97 0.09119045132319313 98-99 0.07964403446500465 100-101 0.06489611111431845 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1905353.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.293150263563454 #Duplication Level Percentage of deduplicated Percentage of total 1 64.34703277201365 25.28397627725174 2 15.322141352184524 12.041104050218916 3 6.6221416686802375 7.806144229621721 4 3.881893733567501 6.101273351210126 5 2.3296358971016047 4.57693666821024 6 1.5093369896131985 3.5583963078735548 7 1.1177337447844202 3.0743495991928786 8 0.80157274368261 2.51970546117581 9 0.6007280911546435 2.1244049237954745 >10 3.233457407530818 22.399942762586626 >50 0.1671596557611228 4.450062270818301 >100 0.0625900838656066 4.289818875940879 >500 0.0029608506271276733 0.7826057536940307 >1k 0.0016150094329787307 0.9912794684096633 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4016 0.21077459137493157 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2127 0.11163285753348591 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.248371299176583E-5 0.0 2 0.0 0.0 0.0 5.248371299176583E-5 0.0 3 0.0 0.0 0.0 5.248371299176583E-5 0.0 4 0.0 0.0 0.0 1.574511389752975E-4 0.0 5 0.0 0.0 0.0 2.6241856495882917E-4 0.0 6 0.0 0.0 0.0 2.6241856495882917E-4 0.0 7 0.0 0.0 0.0 2.6241856495882917E-4 0.0 8 0.0 0.0 0.0 2.6241856495882917E-4 0.0 9 0.0 0.0 0.0 9.44706833851785E-4 0.0 10-11 0.0 0.0 0.0 0.001495785820265326 0.0 12-13 0.0 0.0 0.0 0.002230557802150048 0.0 14-15 0.0 0.0 0.0 0.003700101765919491 0.0 16-17 0.0 0.0 0.0 0.007085301253888387 0.0 18-19 0.0 0.0 0.0 0.010575468167840815 0.0 20-21 0.0 0.0 0.0 0.015981290605992696 0.0 22-23 0.0 0.0 0.0 0.024746070675617588 0.0 24-25 0.0 0.0 0.0 0.03663363166825255 0.0 26-27 0.0 0.0 0.0 0.04972831805969813 0.0 28-29 0.0 0.0 0.0 0.07043314283494974 0.0 30-31 0.0 0.0 0.0 0.11310240149725537 0.0 32-33 0.0 0.0 0.0 0.2051588340848126 0.0 34-35 0.0 0.0 0.0 0.34353214338760324 0.0 36-37 0.0 0.0 0.0 0.5310302080506867 0.0 38-39 0.0 0.0 0.0 0.8085640823511444 0.0 40-41 0.0 0.0 0.0 1.1337006843351336 0.0 42-43 0.0 0.0 0.0 1.448996590133167 0.0 44-45 0.0 0.0 0.0 1.7986693279408068 0.0 46-47 0.0 0.0 0.0 2.197361853682756 0.0 48-49 0.0 0.0 0.0 2.591986891667843 0.0 50-51 0.0 0.0 0.0 3.003275508527816 0.0 52-53 0.0 0.0 0.0 3.4059305546006433 0.0 54-55 0.0 0.0 0.0 3.8179277015860054 0.0 56-57 0.0 0.0 0.0 4.294899685255173 0.0 58-59 0.0 0.0 0.0 4.777225007649502 0.0 60-61 0.0 0.0 0.0 5.268997398382346 0.0 62-63 0.0 0.0 0.0 5.751795074193601 0.0 64-65 0.0 0.0 0.0 6.235248796417252 0.0 66-67 0.0 0.0 0.0 6.767249953158286 0.0 68-69 0.0 0.0 0.0 7.351813548460575 0.0 70-71 0.0 0.0 0.0 7.959522461192231 0.0 72-73 0.0 0.0 0.0 8.56376744886643 0.0 74-75 0.0 0.0 0.0 9.146205453792552 0.0 76-77 0.0 0.0 0.0 9.747668804678188 0.0 78-79 0.0 0.0 0.0 10.43712110039452 0.0 80-81 0.0 0.0 0.0 11.167143306253486 0.0 82-83 0.0 0.0 0.0 11.904093362227368 0.0 84-85 0.0 0.0 0.0 12.611600055212865 0.0 86-87 0.0 0.0 0.0 13.34254072604919 0.0 88-89 0.0 0.0 0.0 14.139322214833683 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATGGG 4290 0.0 23.272022 3 GTACATG 5000 0.0 23.050684 1 GTATTGC 540 0.0 22.048786 1 TACACCG 145 6.1120844E-4 19.672312 5 TACCGTC 145 6.130521E-4 19.663515 7 TACATGG 5570 0.0 18.965252 2 CGCCGTA 680 0.0 18.861353 94-95 TAATACT 2295 0.0 18.850845 4 TAAGGCG 130 0.0075269747 18.28516 5 GTATAAA 1535 0.0 17.995258 1 ATACAGC 1125 0.0 17.741793 6 GTATAGA 970 0.0 17.6754 1 GTCCCAC 755 0.0 17.625225 9 GTCGGAC 95 1.893489E-4 17.467916 24-25 GTATATA 1620 0.0 17.345045 1 CATGGGA 2175 0.0 17.049335 4 CATGGGG 2160 0.0 16.947634 4 GTATAGT 815 0.0 16.946453 1 CATGGGC 1265 0.0 16.911966 4 GTATACA 1495 0.0 16.883913 1 >>END_MODULE