FastQCFastQC Report
Fri 10 Feb 2017
SRR2031613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905353
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44740.23481213192516034No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT35000.18369299547118043No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC20970.11005834614373296No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC753.077064E-431.670423
GCACGTA1056.91372E-527.1396649
ATGGGCG1351.3445568E-524.6267325
TATACCG2002.0583684E-823.7472045
ACATGGG41950.023.4413973
GTACATG49900.022.6740671
GTACGGG1050.002186209422.6377721
TAATACT21400.021.9769044
GTATAGA8150.019.8323541
CTCGCCG600.004768732519.78933756-57
TACATGG54400.019.5621682
GGACCGT1250.0060366718.9977656
GTATAGG10850.018.8404671
TATACAG18900.018.3444025
GTGTAGG8650.018.1363871
TTAAGCG1051.9478019E-518.10593844-45
CATGGGA22950.018.008674
TCTATAC10350.017.9006713
TCTGCGC1901.864799E-417.5020753
GTATATA18200.017.500741
Produced by FastQC (version 0.11.5)