FastQCFastQC Report
Fri 10 Feb 2017
SRR2031612_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031612_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577376
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16860.1068863733187268No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCCG902.4020843E-531.6886885
GTACATG45750.030.9135061
TACATGG46700.029.3398362
ACATGGG49400.026.747663
CATGGGG33750.025.350954
GCACCGT2351.7644197E-1024.2621636
GTATCAA42900.023.7541431
GGTATCA33100.023.5938451
GTATAGA4200.022.6758521
TGCACCG1903.2665776E-722.5156485
TCAACGC44100.022.4192094
CAACGCA45600.021.6817325
ATCAACG45600.021.6803553
ACCGTGC3151.4551915E-1121.1163988
AACGCAG48050.021.0621326
TATCAAC48100.020.968772
ATGGGGG20000.019.9638735
GAGTACA33450.019.5032651
GTCTTAG7100.019.450131
AGTACAT32500.019.176532
Produced by FastQC (version 0.11.5)