##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031612_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1577376 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97307300225184 33.0 31.0 34.0 30.0 34.0 2 32.21138840707606 34.0 31.0 34.0 30.0 34.0 3 32.36781147931755 34.0 31.0 34.0 30.0 34.0 4 35.44142106891445 37.0 35.0 37.0 33.0 37.0 5 35.14302804150691 37.0 35.0 37.0 32.0 37.0 6 35.35820248311119 37.0 35.0 37.0 33.0 37.0 7 35.515062990688335 37.0 35.0 37.0 33.0 37.0 8 35.58670285334632 37.0 35.0 37.0 33.0 37.0 9 37.53469115797375 39.0 38.0 39.0 35.0 39.0 10-11 37.48970917523786 39.0 38.0 39.0 35.0 39.0 12-13 37.50966510204289 39.0 38.0 39.0 35.0 39.0 14-15 38.949155115837954 41.0 39.0 41.0 36.0 41.0 16-17 38.89701187288256 41.0 39.0 41.0 35.0 41.0 18-19 38.812468935751525 41.0 39.0 41.0 35.0 41.0 20-21 38.80161166392794 41.0 39.0 41.0 35.0 41.0 22-23 38.74164498508916 41.0 39.0 41.0 34.5 41.0 24-25 38.67234888828028 41.0 38.5 41.0 34.0 41.0 26-27 38.57804416955754 40.0 38.0 41.0 34.0 41.0 28-29 38.46610351621934 40.0 38.0 41.0 34.0 41.0 30-31 38.34565823240622 40.0 38.0 41.0 34.0 41.0 32-33 38.20167797658897 40.0 38.0 41.0 33.5 41.0 34-35 38.08209583510843 40.0 38.0 41.0 33.0 41.0 36-37 37.96073510691173 40.0 38.0 41.0 33.0 41.0 38-39 37.79343574391901 40.0 38.0 41.0 33.0 41.0 40-41 37.64675638528797 40.0 37.5 41.0 32.0 41.0 42-43 37.4757125124257 40.0 37.0 41.0 31.5 41.0 44-45 37.27859559166616 40.0 37.0 41.0 31.0 41.0 46-47 37.10092615837949 40.0 37.0 41.0 31.0 41.0 48-49 37.25396291055525 40.0 37.0 41.0 31.0 41.0 50-51 37.011662089444755 39.5 36.5 40.5 30.5 41.0 52-53 37.13123345353296 39.5 37.0 40.5 31.0 41.0 54-55 37.39875907836813 40.0 37.0 41.0 31.0 41.0 56-57 37.2890921378289 40.0 36.5 41.0 31.0 41.0 58-59 37.087620833586925 40.0 36.0 41.0 31.0 41.0 60-61 36.78742481183941 39.0 35.5 41.0 30.5 41.0 62-63 36.45775864473657 39.0 35.0 41.0 30.0 41.0 64-65 36.172546685127706 39.0 35.0 41.0 29.0 41.0 66-67 35.93189195220417 38.0 35.0 40.5 29.0 41.0 68-69 35.66283783955126 37.0 35.0 40.0 29.0 41.0 70-71 35.33467321678535 37.0 35.0 39.5 29.0 41.0 72-73 34.95296143722233 36.0 34.5 39.0 29.0 41.0 74-75 34.52673015184712 36.0 34.0 39.0 28.0 40.5 76-77 34.12834004067515 35.0 34.0 37.5 27.5 39.5 78-79 33.78163196346337 35.0 34.0 37.0 27.0 39.0 80-81 33.456798505873046 35.0 34.0 37.0 26.0 39.0 82-83 33.14352380155397 35.0 34.0 36.0 26.0 38.0 84-85 32.886694738603865 35.0 33.5 36.0 26.0 37.0 86-87 32.6530836655306 35.0 33.0 35.5 26.0 37.0 88-89 32.42087460440631 35.0 33.0 35.0 25.0 36.0 90-91 32.231232756172275 35.0 33.0 35.0 25.0 36.0 92-93 32.05821915637109 35.0 33.0 35.0 25.0 36.0 94-95 31.904314190148703 35.0 33.0 35.0 24.0 36.0 96-97 31.801965098999858 35.0 32.0 35.0 24.0 35.0 98-99 31.698725605055486 35.0 32.0 35.0 24.0 35.0 100-101 31.23426310530907 34.5 31.5 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14985867996580637 1101 2 0.16483213070841174 1101 3 0.1518019012203098 1101 4 0.14557172846443223 1101 5 0.2401754097376667 1101 6 0.1696661197611533 1101 7 0.145073712251218 1101 8 0.23396832293688163 1101 9 0.17104020062309644 1101 10-11 0.2501928285098316 1101 12-13 0.25777769355229196 1101 14-15 0.2365664712466682 1101 16-17 0.2590365484750592 1101 18-19 0.36657250830904076 1101 20-21 0.22390813613153426 1101 22-23 0.24590694138087343 1101 24-25 0.3386348622228965 1101 26-27 0.4741653525955343 1101 28-29 0.40305139205131724 1101 30-31 0.41837426909536646 1101 32-33 0.48521223380245715 1101 34-35 0.4008342283548316 1101 36-37 0.5373637137569602 1101 38-39 0.5512328840449428 1101 40-41 0.4590721738852892 1101 42-43 0.3195157907999189 1101 44-45 0.42910084890581857 1101 46-47 0.46241403297125316 1101 48-49 0.34582242791618967 1101 50-51 0.30608332674022876 1101 52-53 0.26381868626694427 1101 54-55 0.3004605643420817 1101 56-57 0.41459194554666823 1101 58-59 0.3175920538965116 1101 60-61 0.45802903400145567 1101 62-63 0.15779390278012784 1101 64-65 0.3833320731220269 1101 66-67 0.28829243583108877 1101 68-69 0.23027084494265182 1101 70-71 0.09390906629601403 1101 72-73 0.37851200664193385 1101 74-75 0.15078675732660685 1101 76-77 0.15296805385882095 1101 78-79 0.3787417904560968 1101 80-81 0.37168335273565845 1101 82-83 0.26053776603856704 1101 84-85 0.2471412686012755 1101 86-87 0.1969646990887739 1101 88-89 0.3840424772862647 1101 90-91 0.20363367287114897 1101 92-93 0.1728476672289858 1101 94-95 0.09708896089492924 1101 96-97 0.0689327496070753 1101 98-99 -0.14214713938743984 1101 100-101 0.10116883408654687 1102 1 0.09372299235110049 1102 2 0.17308443985606914 1102 3 0.17240186324803375 1102 4 0.28644888813786906 1102 5 0.16629767381822091 1102 6 0.0490375119781703 1102 7 0.02723306925394553 1102 8 0.1926834608861796 1102 9 0.25747543623443647 1102 10-11 0.21103058361526195 1102 12-13 0.21295791199282377 1102 14-15 0.28523261163086033 1102 16-17 0.332783546499833 1102 18-19 0.45904333793674823 1102 20-21 0.31702266252725764 1102 22-23 0.2950585606987701 1102 24-25 0.39947378314396786 1102 26-27 0.3591151725602941 1102 28-29 0.2511347911699531 1102 30-31 0.30271953038796084 1102 32-33 0.25639676909169395 1102 34-35 0.1638438979626855 1102 36-37 0.07953459567982435 1102 38-39 0.18931624985457063 1102 40-41 0.2389845045505723 1102 42-43 0.25293189704235886 1102 44-45 0.09482736854647555 1102 46-47 -0.40107978320959603 1102 48-49 0.1872165435451194 1102 50-51 0.029381985386081055 1102 52-53 0.026861664892550152 1102 54-55 -0.06027609789448718 1102 56-57 0.0015376418550303583 1102 58-59 -0.08691682129127543 1102 60-61 -0.2930185346067944 1102 62-63 -0.6784198278631735 1102 64-65 -0.33773321588365945 1102 66-67 -0.14935974474376223 1102 68-69 -0.05906044321699255 1102 70-71 -0.014352735921697501 1102 72-73 -0.058001005525852634 1102 74-75 -0.009290552903593152 1102 76-77 -0.034238618603616544 1102 78-79 -0.021398607053718877 1102 80-81 -0.0068011868837487555 1102 82-83 -0.21735670433140086 1102 84-85 -0.07339973646502784 1102 86-87 -0.07324138380404577 1102 88-89 -0.2505209580725918 1102 90-91 -0.19987426633323224 1102 92-93 -0.16075380369723646 1102 94-95 -0.18613095523296508 1102 96-97 -0.3275153432851141 1102 98-99 -0.3642441752179515 1102 100-101 -0.01622713352376337 1103 1 -0.1419169938018925 1103 2 -0.17014095355325765 1103 3 -0.16611471353821372 1103 4 -0.1556512273928874 1103 5 -0.08007270025951385 1103 6 -0.10101900216287873 1103 7 -0.011730407179037172 1103 8 -0.0020536336613545814 1103 9 0.23068648214226783 1103 10-11 0.17411652681750667 1103 12-13 0.13390901787655451 1103 14-15 0.243556039349059 1103 16-17 0.1774134518647159 1103 18-19 0.059506607915857046 1103 20-21 -0.1353172523053061 1103 22-23 0.039977501486127665 1103 24-25 0.1298794337239073 1103 26-27 0.010553780685818026 1103 28-29 -0.024756401382688864 1103 30-31 0.11678904903762088 1103 32-33 -0.010789473155860207 1103 34-35 0.14736512517455935 1103 36-37 0.16384910625902194 1103 38-39 -0.00431975736923107 1103 40-41 -0.32243552272635867 1103 42-43 -0.20648062699179093 1103 44-45 -0.21603377349795494 1103 46-47 0.11978039955421593 1103 48-49 -0.22367410680543998 1103 50-51 -0.09285069855598493 1103 52-53 -0.10189412728556135 1103 54-55 -0.23148370707522048 1103 56-57 -0.10948605654714783 1103 58-59 -0.06480540293230774 1103 60-61 -0.003543094276132308 1103 62-63 0.12633893391408435 1103 64-65 0.043879042888541164 1103 66-67 -0.13890202190913215 1103 68-69 -0.06518784819607504 1103 70-71 -0.11235367851918454 1103 72-73 -0.02755583675502038 1103 74-75 -0.05286118306754872 1103 76-77 -0.5590193395655731 1103 78-79 -0.26254507252170356 1103 80-81 -0.23319349929224842 1103 82-83 -0.36811547445022086 1103 84-85 -0.5264179252597998 1103 86-87 -0.38187584783727857 1103 88-89 -0.37144557946955814 1103 90-91 -0.3305136894302052 1103 92-93 -0.40801620448468157 1103 94-95 -0.507276732521369 1103 96-97 -0.35454672427383827 1103 98-99 -0.25195307942752265 1103 100-101 -0.31377721422845894 1104 1 -0.0271201383173576 1104 2 0.038136256872952856 1104 3 0.1965188589807454 1104 4 0.03142580997068478 1104 5 -0.044852004580363314 1104 6 0.009204346599155144 1104 7 0.11633594438301742 1104 8 0.1511272086312374 1104 9 0.21396593748476533 1104 10-11 0.2804439254085196 1104 12-13 0.2314904267043758 1104 14-15 0.21868848940987817 1104 16-17 0.18020327055711505 1104 18-19 0.3042065646501939 1104 20-21 0.213584575464715 1104 22-23 0.3191522294430982 1104 24-25 0.20167491776867763 1104 26-27 0.28401315586438614 1104 28-29 0.2737868520316269 1104 30-31 0.2603157308880881 1104 32-33 0.11288602623596944 1104 34-35 0.19647368932610476 1104 36-37 0.15982764485934098 1104 38-39 0.1852215858254027 1104 40-41 0.3095533098355361 1104 42-43 0.23421276642365996 1104 44-45 0.20128085764189052 1104 46-47 0.21111992069316443 1104 48-49 0.15568001037102874 1104 50-51 0.11125504924604712 1104 52-53 0.05051834138765798 1104 54-55 0.14455507691484826 1104 56-57 0.16747028595360547 1104 58-59 0.07842331730214624 1104 60-61 0.2172842185998931 1104 62-63 0.19021190430567714 1104 64-65 0.33433753642726316 1104 66-67 0.2175131325002937 1104 68-69 0.2051970120884974 1104 70-71 0.10461646001255787 1104 72-73 0.08461242584304074 1104 74-75 0.01020769912741315 1104 76-77 0.15448138288930835 1104 78-79 0.1143753513184933 1104 80-81 0.021063678317354118 1104 82-83 -0.07353960036144969 1104 84-85 0.04226324764773892 1104 86-87 -0.03243626057533788 1104 88-89 0.1717037523617222 1104 90-91 0.07741208955575019 1104 92-93 0.0448487168616154 1104 94-95 0.007906044480890273 1104 96-97 -0.09257213672618647 1104 98-99 0.0014561320762425112 1104 100-101 -0.0321361396074451 1105 1 0.1253208444994094 1105 2 0.1994023085356602 1105 3 0.1717105272830608 1105 4 0.11852262253722756 1105 5 -0.02149196556270283 1105 6 0.112263604668847 1105 7 0.2655844380991539 1105 8 0.26566574749127625 1105 9 0.3321564340677412 1105 10-11 0.34927574502322045 1105 12-13 0.3324716672994015 1105 14-15 0.3592070793021662 1105 16-17 0.4272040854076167 1105 18-19 0.39135319015799297 1105 20-21 0.28367064033157874 1105 22-23 0.20559284944321377 1105 24-25 0.30292529694181525 1105 26-27 0.3547096151307869 1105 28-29 0.4145567370603871 1105 30-31 0.29045553425336124 1105 32-33 0.33191724631102915 1105 34-35 0.32898354782761174 1105 36-37 0.310422574942784 1105 38-39 0.2046449155305723 1105 40-41 0.12135406996615217 1105 42-43 0.1733971129670948 1105 44-45 0.13224383844828935 1105 46-47 0.38803615763027466 1105 48-49 0.19387464068465476 1105 50-51 0.20770108175679525 1105 52-53 0.21047290008959862 1105 54-55 0.17742343989804255 1105 56-57 0.2416112729338451 1105 58-59 0.13972817845043295 1105 60-61 0.2608274205460148 1105 62-63 0.3662731429974002 1105 64-65 0.2758251738961093 1105 66-67 0.20258040227528085 1105 68-69 0.2190792096867682 1105 70-71 0.276578911463524 1105 72-73 0.28523590543311883 1105 74-75 0.2214948655294009 1105 76-77 -0.02444595861631882 1105 78-79 0.07422835109841941 1105 80-81 0.19069830411064714 1105 82-83 0.22626218874096082 1105 84-85 0.13834051569786965 1105 86-87 0.2373082510794049 1105 88-89 0.18064629053181847 1105 90-91 0.2813373493265132 1105 92-93 0.04289857738537961 1105 94-95 0.011989218710201754 1105 96-97 0.15735351560029898 1105 98-99 6.345902839264284E-4 1105 100-101 0.05481941237942323 1106 1 0.071642284494434 1106 2 0.06971761500534512 1106 3 0.09940948502013924 1106 4 0.2158117918883562 1106 5 0.25943157288533314 1106 6 0.07381579696777862 1106 7 0.12035191704475778 1106 8 0.27578058628661495 1106 9 0.1824050108271038 1106 10-11 0.23008865532343492 1106 12-13 0.32219643393807473 1106 14-15 0.32184605504242825 1106 16-17 0.3539012154358474 1106 18-19 0.38285134968257495 1106 20-21 0.297879138411723 1106 22-23 0.35106120389709616 1106 24-25 0.3655355827133988 1106 26-27 0.3056260925040064 1106 28-29 0.31913427262920635 1106 30-31 0.22962931886411297 1106 32-33 0.28778135056425214 1106 34-35 0.49165195631891123 1106 36-37 0.5305893017283978 1106 38-39 0.5255712301276816 1106 40-41 0.41031706687014236 1106 42-43 0.34543339974236886 1106 44-45 0.51638812763575 1106 46-47 0.6343560192127811 1106 48-49 0.40275664645700715 1106 50-51 0.22920904136614695 1106 52-53 0.2636138713611018 1106 54-55 0.3723539038631074 1106 56-57 0.305266328519501 1106 58-59 0.3359694211624529 1106 60-61 0.3709381740383648 1106 62-63 0.4691756579191946 1106 64-65 0.5730054327836172 1106 66-67 0.397531292755275 1106 68-69 0.3638811490915117 1106 70-71 0.3498699433414245 1106 72-73 0.3605259503389462 1106 74-75 0.4672369817070603 1106 76-77 0.3245085519991946 1106 78-79 0.3989556223063673 1106 80-81 0.2581039463783057 1106 82-83 0.33828932072363926 1106 84-85 0.3718160454293624 1106 86-87 0.4201719834771964 1106 88-89 0.46318677333106706 1106 90-91 0.3755372404160511 1106 92-93 0.43977415623335503 1106 94-95 0.272919377206037 1106 96-97 0.39930326358818036 1106 98-99 0.44630516723548297 1106 100-101 0.44670302952525276 1107 1 0.04539357147958967 1107 2 0.01955186545618659 1107 3 0.045521707983596116 1107 4 0.2090438459702213 1107 5 0.10485239078877129 1107 6 -0.021513009262712046 1107 7 0.1190202649864176 1107 8 0.15189709812945296 1107 9 0.2632347483142681 1107 10-11 0.19881711184529394 1107 12-13 0.16210362104590104 1107 14-15 0.10192466021830171 1107 16-17 0.19681147502659257 1107 18-19 0.3750137546770347 1107 20-21 0.26606880713970327 1107 22-23 0.2910430286460368 1107 24-25 0.2305087204464158 1107 26-27 0.28005048932946863 1107 28-29 0.2579114835550982 1107 30-31 0.3455820220792347 1107 32-33 0.42085829374581607 1107 34-35 0.38802796036014087 1107 36-37 0.5286108900876698 1107 38-39 0.6145132051459257 1107 40-41 0.6142442836016571 1107 42-43 0.5089253959050808 1107 44-45 0.5274608059316677 1107 46-47 0.6330070145455693 1107 48-49 0.5622701821346112 1107 50-51 0.36177997390391425 1107 52-53 0.4101020246707634 1107 54-55 0.5247631529321737 1107 56-57 0.5836593741080165 1107 58-59 0.5455424484386171 1107 60-61 0.5420726019142847 1107 62-63 0.4814771579986825 1107 64-65 0.5909968747340031 1107 66-67 0.5620057465015762 1107 68-69 0.6202950803657572 1107 70-71 0.5731230390682711 1107 72-73 0.6113300616647237 1107 74-75 0.6465481302655292 1107 76-77 0.554986545677707 1107 78-79 0.5858091572898658 1107 80-81 0.6260156734427582 1107 82-83 0.6099761185588903 1107 84-85 0.6586709514831881 1107 86-87 0.5435995907495297 1107 88-89 0.651406665590649 1107 90-91 0.834641640137896 1107 92-93 0.727372225252175 1107 94-95 0.7646786018314842 1107 96-97 0.8495544051113342 1107 98-99 0.6348590436583486 1107 100-101 0.6952365198461408 1108 1 0.20264252326348142 1108 2 0.16818970510821885 1108 3 0.12173842056394335 1108 4 0.023714665895901987 1108 5 0.21288857134202743 1108 6 0.30412911879665216 1108 7 0.13397150747312736 1108 8 0.1733011626233747 1108 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-0.9764359664153375 2316 76-77 -0.6569845466958881 2316 78-79 -0.9187573756215741 2316 80-81 -0.8948253935350223 2316 82-83 -0.995737645265919 2316 84-85 -1.649413874075254 2316 86-87 -0.9939387828576791 2316 88-89 -0.5847979857913899 2316 90-91 -0.8707831063458755 2316 92-93 -1.219595596894628 2316 94-95 -1.0879210811627473 2316 96-97 -0.8748847951856327 2316 98-99 -0.5469213738700667 2316 100-101 -1.1412477211960876 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 108.0 8 514.0 9 625.0 10 549.0 11 697.0 12 963.0 13 1286.0 14 1631.0 15 2180.0 16 2685.0 17 3409.0 18 4264.0 19 5402.0 20 6796.0 21 8193.0 22 9503.0 23 9930.0 24 10461.0 25 11274.0 26 12694.0 27 14684.0 28 17292.0 29 20550.0 30 24729.0 31 30344.0 32 38126.0 33 49695.0 34 70084.0 35 104621.0 36 180169.0 37 338955.0 38 448401.0 39 146319.0 40 239.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.61501820328455 18.30205416599536 12.926465353262168 24.156462277457923 2 15.287337749085847 19.65936532251703 40.70261866168601 24.350678266711114 3 19.024179565187016 24.112685538625698 29.576238353223623 27.286896542963664 4 12.165518135192329 15.89223646936321 38.21519034788846 33.727055047556 5 13.31862883403615 38.00363227821879 35.355615034429114 13.322123853315947 6 32.69135375905156 36.77613749029028 17.482262093565193 13.05024665709297 7 28.63619822544394 31.985385411195672 21.3702478929449 18.00816847041549 8 25.990516848281004 36.357903240352044 19.73793679846461 17.913643112902346 9 26.060473655899298 15.792552154416065 19.60652939185306 38.54044479783158 10-11 25.044493253285705 26.026552404858755 28.824101433184534 20.10485290867101 12-13 25.388319686772125 23.319694710786578 28.677148876917386 22.61483672552391 14-15 22.378282178857688 25.193077750970904 25.815874374893017 26.61276569527839 16-17 22.685135838186962 28.402648241907446 26.4603398175289 22.45187610237669 18-19 22.657920637618368 27.790753569734434 27.791165779253557 21.76016001339364 20-21 23.165617451834382 26.894450889772216 27.838966127161036 22.100965531232365 22-23 23.02719833444911 26.708977441015964 27.541277412614367 22.72254681192056 24-25 22.796720629704016 26.864615665510318 27.684616730570266 22.654046974215404 26-27 22.846455125474204 27.06228572008196 27.42722724321912 22.664031911224715 28-29 22.73643062909541 27.174053618160794 27.536174000365165 22.553341752378632 30-31 22.995722009210233 27.02456484693567 27.49699500943339 22.482718134420708 32-33 22.74020271641004 27.074394437344047 27.505553526869942 22.67984931937598 34-35 22.901324731706328 26.92851926236991 27.630444485018156 22.539711520905605 36-37 22.943483354634534 26.903160692187534 27.558552938551113 22.594803014626823 38-39 22.84464832734871 27.107138691092043 27.457245450672506 22.590967530886736 40-41 22.945480342036394 27.082033706611487 27.373752358347026 22.59873359300509 42-43 22.841732091777736 26.988555677276693 27.400283762400342 22.76942846854523 44-45 22.896572125779713 27.07609908700902 27.255878704621832 22.771450082589435 46-47 22.81966661161015 27.06312653258287 27.24725882068939 22.869948035117588 48-49 22.842312843937158 27.067239575369072 27.24439120307655 22.84605637761722 50-51 22.732848015565015 27.180924607425165 27.36647919837879 22.719748178631026 52-53 22.86074276573128 27.31193185260658 27.13936825863984 22.687957123022297 54-55 22.83074037662034 27.101766928611287 27.215021415249634 22.852471279518735 56-57 22.85021586774709 27.18534162003151 27.14164446637588 22.82279804584552 58-59 22.93954322449885 27.030838676066193 27.124499768226872 22.905118331208087 60-61 22.882228027463093 27.143609879349036 27.258737751594236 22.715424341593636 62-63 22.77237443720351 27.269567206718285 27.16512615855848 22.792932197519725 64-65 22.7420287573501 27.201316050907785 27.254162490263877 22.80249270147824 66-67 22.71330521946901 27.194071338137444 27.033289226932194 23.059334215461355 68-69 22.682448826238637 27.141434610617388 27.086505282544916 23.089611280599062 70-71 22.91429644684352 26.895694676134646 27.164892425597042 23.025116451424797 72-73 22.795960544409745 27.093980627489174 27.104163329267656 23.005895498833432 74-75 22.707131935360277 27.274822295999492 27.039132177875093 22.97891359076514 76-77 22.727077797384677 27.084563617679247 27.067918049164785 23.120440535771294 78-79 22.730193011759223 27.10474743030836 27.054467749584337 23.110591808348076 80-81 22.78337766429953 27.17899600513698 26.932330979731272 23.10529535083222 82-83 22.766663864173186 27.126275597208714 27.045881048428672 23.061179490189424 84-85 22.862139776676134 26.944945768192973 27.154486403338435 23.038428051792454 86-87 22.765042857238022 27.139872156292753 27.032907494283005 23.06217749218622 88-89 22.760722843247745 26.954072878275614 27.066485689338325 23.218718589138316 90-91 22.760555191840886 26.905421457148503 27.002085285677158 23.331938065333453 92-93 23.01210128836144 26.948972911326013 26.95411645100842 23.08480934930413 94-95 22.78338303796215 26.897663057209204 27.12714228219787 23.19181162263078 96-97 22.654051682264072 26.858958353228658 27.24995899315749 23.237030971349782 98-99 22.88437441511517 26.801794228396137 27.04142650039812 23.272404856090574 100-101 22.921063632863074 26.848597189367222 26.88707163478648 23.34326754298322 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 52.0 1 42.5 2 52.5 3 96.0 4 91.0 5 75.0 6 127.0 7 231.0 8 381.5 9 461.5 10 443.0 11 444.0 12 480.0 13 524.0 14 578.5 15 643.0 16 696.0 17 779.5 18 864.5 19 934.0 20 1093.0 21 1281.5 22 1559.0 23 1971.5 24 2489.5 25 3205.0 26 4290.5 27 6032.5 28 8463.0 29 11949.5 30 16361.5 31 20246.0 32 24044.5 33 28242.5 34 32425.5 35 36761.0 36 40280.5 37 42121.0 38 43773.0 39 46054.5 40 48833.0 41 50940.0 42 52113.5 43 54585.5 44 57134.0 45 58525.5 46 60208.0 47 62470.0 48 65159.5 49 65581.5 50 63674.5 51 61840.0 52 59827.0 53 58076.5 54 55944.5 55 52085.5 56 46625.0 57 41886.0 58 38047.0 59 33122.0 60 27043.0 61 21250.0 62 16359.0 63 12164.5 64 8978.5 65 6636.5 66 4712.5 67 3372.0 68 2490.0 69 1762.5 70 1280.0 71 1014.5 72 770.0 73 572.0 74 468.5 75 321.0 76 216.5 77 166.0 78 109.5 79 85.0 80 61.0 81 41.0 82 33.5 83 28.5 84 26.0 85 22.0 86 17.5 87 14.5 88 13.0 89 11.5 90 8.0 91 11.0 92 10.5 93 3.5 94 2.5 95 2.5 96 2.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4472617815917067 2 0.3441157973748808 3 0.18423001237498224 4 0.21155387174649543 5 0.23507394559065178 6 0.18530775160773336 7 0.17655904489481267 8 0.17712961272391617 9 0.17579828778934128 10-11 0.18892134785872233 12-13 0.17773187876574767 14-15 0.18673417118049215 16-17 0.0783579818635506 18-19 0.03220538413162112 20-21 3.8037855273568254E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.13402004341387214 46-47 0.20515083277544477 48-49 0.0840002637291299 50-51 0.064696052177794 52-53 0.0726206053597874 54-55 0.08111572637088431 56-57 0.11148261416428296 58-59 0.09392180431298562 60-61 0.13763363966486114 62-63 0.23843395613981702 64-65 0.13040644716288316 66-67 0.10403353417320917 68-69 0.10872486965694927 70-71 0.08856480636195808 72-73 0.07439570527255392 74-75 0.14039138417219482 76-77 0.214501805530197 78-79 0.1386479824721563 80-81 0.08571196721644046 82-83 0.08865990100014201 84-85 0.09813766660580611 86-87 0.07319117318889092 88-89 0.09433388107844928 90-91 0.1149060211389041 92-93 0.16387975980362324 94-95 0.13123060069381048 96-97 0.0899912259347169 98-99 0.07677307122715193 100-101 0.06371340758322683 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1577376.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.08523008704488 #Duplication Level Percentage of deduplicated Percentage of total 1 70.08654086433934 38.60733229517206 2 15.44612089492971 17.017062468990293 3 5.92602382605115 9.793091578780128 4 3.0786599360581337 6.783547637501167 5 1.6622371066507349 4.578235673953974 6 1.0226166936901295 3.3798645517664325 7 0.6604063333638208 2.546504437700141 8 0.4563496178584539 2.011049895989435 9 0.33964523010763487 1.6838492083601733 >10 1.2786249391302318 11.259123039059839 >50 0.03409581141625264 1.2798587604147778 >100 0.008100239587667736 0.7077107574740558 >500 2.3140272668448932E-4 0.08961051326855811 >1k 3.4710409002673394E-4 0.26315918156890217 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1686 0.1068863733187268 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.267928509118942E-4 0.0 10-11 0.0 0.0 0.0 1.267928509118942E-4 0.0 12-13 0.0 0.0 0.0 4.120767654636561E-4 0.0 14-15 0.0 0.0 0.0 5.705678291035238E-4 0.0 16-17 0.0 0.0 0.0 7.924553181993387E-4 0.0 18-19 0.0 0.0 0.0 9.509463818392063E-4 0.0 20-21 0.0 0.0 0.0 0.0019335909764063863 0.0 22-23 0.0 0.0 0.0 0.004279258718276429 0.0 24-25 0.0 0.0 0.0 0.006561530034690524 0.0 26-27 0.0 0.0 0.0 0.007797760331081493 0.0 28-29 0.0 0.0 0.0 0.010492108412959243 0.0 30-31 0.0 0.0 0.0 0.01603929564035461 0.0 32-33 0.0 0.0 0.0 0.030271793155214735 0.0 34-35 0.0 0.0 0.0 0.05078053679021362 0.0 36-37 0.0 0.0 0.0 0.08510970117460898 0.0 38-39 0.0 0.0 0.0 0.13769703609031708 0.0 40-41 0.0 0.0 0.0 0.19858930274075426 0.0 42-43 0.0 0.0 0.0 0.25095475016736657 0.0 44-45 0.0 0.0 0.0 0.3142243827724017 0.0 46-47 0.0 0.0 0.0 0.3874789523867486 0.0 48-49 0.0 0.0 0.0 0.4655199521230195 0.0 50-51 0.0 0.0 0.0 0.5449556732193213 0.0 52-53 0.0 0.0 0.0 0.6271491388229566 0.0 54-55 0.0 0.0 0.0 0.7122271417848376 0.0 56-57 0.0 0.0 0.0 0.8142953867689124 0.0 58-59 0.0 0.0 0.0 0.9268240419532185 0.0 60-61 0.0 0.0 0.0 1.043727050493985 0.0 62-63 0.0 0.0 0.0 1.161264023289311 0.0 64-65 0.0 0.0 0.0 1.2860598867993427 0.0 66-67 0.0 0.0 0.0 1.4274656137788329 0.0 68-69 0.0 0.0 0.0 1.5910600896679041 0.0 70-71 0.0 0.0 0.0 1.7661293185645022 0.0 72-73 0.0 0.0 0.0 1.9463653561357597 0.0 74-75 0.0 0.0 0.0 2.129517629277991 0.0 76-77 0.0 0.0 0.0 2.3225914430040775 0.0 78-79 0.0 0.0 0.0 2.553576319152821 0.0 80-81 0.0 0.0 0.0 2.8115363743330697 0.0 82-83 0.0 0.0 0.0 3.080590803968109 0.0 84-85 0.0 0.0 0.0 3.3544633619378006 0.0 86-87 0.0 0.0 0.0 3.6340098999857995 0.0 88-89 0.0 0.0 0.0 3.957046385896578 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACCCG 90 2.4020843E-5 31.688688 5 GTACATG 4575 0.0 30.913506 1 TACATGG 4670 0.0 29.339836 2 ACATGGG 4940 0.0 26.74766 3 CATGGGG 3375 0.0 25.35095 4 GCACCGT 235 1.7644197E-10 24.262163 6 GTATCAA 4290 0.0 23.754143 1 GGTATCA 3310 0.0 23.593845 1 GTATAGA 420 0.0 22.675852 1 TGCACCG 190 3.2665776E-7 22.515648 5 TCAACGC 4410 0.0 22.419209 4 CAACGCA 4560 0.0 21.681732 5 ATCAACG 4560 0.0 21.680355 3 ACCGTGC 315 1.4551915E-11 21.116398 8 AACGCAG 4805 0.0 21.062132 6 TATCAAC 4810 0.0 20.96877 2 ATGGGGG 2000 0.0 19.963873 5 GAGTACA 3345 0.0 19.503265 1 GTCTTAG 710 0.0 19.45013 1 AGTACAT 3250 0.0 19.17653 2 >>END_MODULE