FastQCFastQC Report
Fri 10 Feb 2017
SRR2031612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577376
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19560.1240034081918325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18080.11462073722435233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG42400.028.3506261
TACATGG43000.026.9520422
ACATGGG42900.026.3505653
CGCGAAA559.489321E-525.90605772-73
GTATCAA42000.024.7743781
GGTATCA31600.024.0569671
CATGGGG31000.023.1358534
CATACCG1252.2582103E-422.7973295
CAACGCA44950.022.5057055
TCAACGC44850.022.4514144
CCTAGAC6200.022.2165493
ATCAACG45850.022.0653343
AACGCAG47000.021.6251266
GCGCGAA550.00288042421.5883872-73
GTATTAG4500.021.116671
TATCAAC50050.020.1187972
GTATAGA5400.019.3569491
GTCTACG1759.919687E-519.0050051
ACGCAGA53050.018.979877
ATGGGGG17800.018.944415
Produced by FastQC (version 0.11.5)