##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031612_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1577376 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.43922755259367 34.0 31.0 34.0 31.0 34.0 2 32.668032225670984 34.0 31.0 34.0 31.0 34.0 3 32.78196003996511 34.0 31.0 34.0 31.0 34.0 4 36.23469610289494 37.0 37.0 37.0 35.0 37.0 5 35.889196995516606 37.0 35.0 37.0 35.0 37.0 6 35.94594123404946 37.0 36.0 37.0 35.0 37.0 7 35.92130665104579 37.0 36.0 37.0 35.0 37.0 8 36.03568204410362 37.0 36.0 37.0 35.0 37.0 9 37.947790507780006 39.0 39.0 39.0 35.0 39.0 10-11 37.922120344166515 39.0 38.0 39.0 35.0 39.0 12-13 37.93533120828515 39.0 38.0 39.0 35.0 39.0 14-15 39.40027773973992 41.0 39.0 41.0 36.0 41.0 16-17 39.36660916610878 41.0 39.0 41.0 36.0 41.0 18-19 39.362803795670786 41.0 39.0 41.0 36.0 41.0 20-21 39.37498732071491 41.0 39.0 41.0 36.0 41.0 22-23 39.3086550067961 41.0 39.0 41.0 36.0 41.0 24-25 39.23936366471912 41.0 39.0 41.0 36.0 41.0 26-27 39.139080663075895 41.0 39.0 41.0 36.0 41.0 28-29 39.0417890217678 40.5 39.0 41.0 35.5 41.0 30-31 38.945218514799265 40.0 38.5 41.0 35.0 41.0 32-33 38.8073420668249 40.0 38.0 41.0 35.0 41.0 34-35 38.688188485180454 40.0 38.0 41.0 35.0 41.0 36-37 38.55642884131621 40.0 38.0 41.0 34.5 41.0 38-39 38.44725195514576 40.0 38.0 41.0 34.0 41.0 40-41 38.3330994005234 40.0 38.0 41.0 34.0 41.0 42-43 38.189826014849984 40.0 38.0 41.0 33.5 41.0 44-45 38.126882556853914 40.0 38.0 41.0 33.0 41.0 46-47 38.14799324954862 40.0 38.0 41.0 33.0 41.0 48-49 38.25746524607956 40.0 38.0 41.0 34.0 41.0 50-51 38.17711186172479 40.0 38.0 41.0 33.5 41.0 52-53 38.0762240581827 40.0 38.0 41.0 33.0 41.0 54-55 37.95753770819386 40.0 37.0 41.0 33.0 41.0 56-57 37.829454106059686 40.0 37.0 41.0 33.0 41.0 58-59 37.624269673178745 40.0 36.5 41.0 33.0 41.0 60-61 37.32680001470797 39.0 36.0 41.0 32.0 41.0 62-63 37.113981067291505 39.0 35.5 41.0 32.0 41.0 64-65 36.79800821110503 39.0 35.0 41.0 31.5 41.0 66-67 36.49186180086422 38.0 35.0 40.5 31.0 41.0 68-69 36.18619720345688 37.0 35.0 40.0 31.0 41.0 70-71 35.832131020124564 37.0 35.0 39.5 31.0 41.0 72-73 35.44797118759256 36.0 35.0 39.0 30.5 41.0 74-75 35.08639981843264 36.0 34.5 39.0 30.0 40.5 76-77 34.472626691416636 35.0 34.0 37.0 29.5 39.0 78-79 34.43462148530217 35.0 34.0 37.0 30.0 39.0 80-81 34.17155706692634 35.0 34.0 37.0 30.0 39.0 82-83 33.902061398170126 35.0 34.0 36.0 29.5 38.0 84-85 33.64323978556793 35.0 34.0 36.0 29.0 37.0 86-87 33.41387849187511 35.0 34.0 35.5 29.0 37.0 88-89 33.204599600856106 35.0 34.0 35.0 29.0 36.0 90-91 33.05806510305723 35.0 34.0 35.0 29.0 36.0 92-93 32.93746069421621 35.0 34.0 35.0 29.0 36.0 94-95 32.82389614143996 35.0 34.0 35.0 29.0 36.0 96-97 32.73013916783316 35.0 33.0 35.0 28.5 35.0 98-99 32.64481899052604 35.0 33.0 35.0 27.5 35.0 100-101 32.21202712606252 34.5 32.5 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19067769128064072 1101 2 0.1712382985405867 1101 3 0.20923468312353322 1101 4 0.13483209904838134 1101 5 0.14381992126808285 1101 6 0.08470190339249228 1101 7 0.22216623548458614 1101 8 0.18806175870108888 1101 9 0.21427926532291508 1101 10-11 0.052262545791840864 1101 12-13 0.18757207819352573 1101 14-15 0.16984786828287213 1101 16-17 0.17859111488558455 1101 18-19 0.18472280293174492 1101 20-21 0.24497395587424364 1101 22-23 0.18390999217682236 1101 24-25 0.23622828156437947 1101 26-27 0.18448136837015028 1101 28-29 0.13559004515082052 1101 30-31 0.1876200913569619 1101 32-33 -0.02277787764993633 1101 34-35 -0.06410137400692406 1101 36-37 0.12673548289745895 1101 38-39 0.09907448555144072 1101 40-41 0.007542011496063594 1101 42-43 0.0639205392611899 1101 44-45 0.09480300299895816 1101 46-47 0.07953132455133272 1101 48-49 0.02828813307937139 1101 50-51 0.2054947285293096 1101 52-53 0.09047927799474564 1101 54-55 0.2018974051449689 1101 56-57 0.2172861082442168 1101 58-59 0.1347318500304766 1101 60-61 0.010395385883121833 1101 62-63 -0.0052177048757684474 1101 64-65 0.19271825339431103 1101 66-67 0.04931351187236288 1101 68-69 0.11487322736100936 1101 70-71 0.13559828024605736 1101 72-73 0.04441580308120052 1101 74-75 0.01637580877727629 1101 76-77 -0.008092177707531789 1101 78-79 0.06590815599236066 1101 80-81 0.19054113788236293 1101 82-83 0.09388995611190865 1101 84-85 0.03145172095780424 1101 86-87 -0.05576299618994085 1101 88-89 -0.12692836277953745 1101 90-91 0.057323263233527655 1101 92-93 -0.17399733333557066 1101 94-95 -0.1881885566319923 1101 96-97 -0.15334946313434017 1101 98-99 -0.22235963533033498 1101 100-101 -0.20314607277615693 1102 1 0.06435490691323054 1102 2 0.1925727244097004 1102 3 0.1800110164002433 1102 4 0.11703009451169777 1102 5 -0.014266342426090262 1102 6 -0.00542563683647046 1102 7 0.14710769488588937 1102 8 0.09916998303066293 1102 9 0.136534352442645 1102 10-11 0.17936559390005158 1102 12-13 0.1983592035919841 1102 14-15 0.1822962809416282 1102 16-17 0.17321837032245213 1102 18-19 0.185347347014563 1102 20-21 0.16800514310756398 1102 22-23 0.15922134822024248 1102 24-25 0.21921986437559582 1102 26-27 0.20547604225820493 1102 28-29 -0.1431256590517762 1102 30-31 -0.1769945732711946 1102 32-33 0.12863242616161585 1102 34-35 0.11225405319788706 1102 36-37 0.15035157648247122 1102 38-39 0.07985601503492035 1102 40-41 0.020709898938228832 1102 42-43 0.10422528346459359 1102 44-45 0.12312982441932263 1102 46-47 0.2623203885346257 1102 48-49 0.07770872271560592 1102 50-51 0.08515757465164597 1102 52-53 -0.08677799086564875 1102 54-55 -0.2194150367724248 1102 56-57 -0.15422685396003288 1102 58-59 -0.1781605718348942 1102 60-61 0.07699676687499846 1102 62-63 -0.019630516856352642 1102 64-65 0.07649476320880666 1102 66-67 0.06175109910034848 1102 68-69 -0.08018104968341788 1102 70-71 -0.09763396021840265 1102 72-73 0.017572065676169757 1102 74-75 0.07346246884875285 1102 76-77 -0.09598468284544737 1102 78-79 -0.10654511978299297 1102 80-81 -0.14981706729452782 1102 82-83 -0.38354401708765096 1102 84-85 -0.32905760723262034 1102 86-87 -0.39587971098814023 1102 88-89 -0.20385970290124789 1102 90-91 -0.11949432659257297 1102 92-93 -0.04384734282863434 1102 94-95 -0.10487604143475693 1102 96-97 -0.07441208741860805 1102 98-99 0.011890195626406808 1102 100-101 0.10303874563972926 1103 1 0.02371268939008786 1103 2 0.04746797513646328 1103 3 0.04336357705388849 1103 4 0.04672212372387463 1103 5 -0.021640290866841383 1103 6 0.003881470598784631 1103 7 0.15746792561373724 1103 8 0.06025134253984277 1103 9 0.1176104567623284 1103 10-11 0.09811765322530874 1103 12-13 0.025047954566908004 1103 14-15 0.02457282069129718 1103 16-17 -0.05547882232195178 1103 18-19 0.03469597461765517 1103 20-21 -0.07355162336600074 1103 22-23 -0.1048388904320916 1103 24-25 -0.0598661497299986 1103 26-27 -0.07224456100417598 1103 28-29 -0.07082405288235094 1103 30-31 -0.14677462690749365 1103 32-33 -0.010427291142555362 1103 34-35 -0.1104804804874675 1103 36-37 -0.08084600195957137 1103 38-39 0.02866327470384533 1103 40-41 0.004135198087801939 1103 42-43 0.01583747967501381 1103 44-45 0.012519033763545906 1103 46-47 -0.04969918913165117 1103 48-49 -0.1618716128657809 1103 50-51 -0.0709319804506876 1103 52-53 -0.02648616101701151 1103 54-55 0.06682804493743078 1103 56-57 0.051068583617905006 1103 58-59 0.11489392364605777 1103 60-61 0.14374549374277734 1103 62-63 -0.12393299471991526 1103 64-65 0.011392453844976558 1103 66-67 -0.027674187536867123 1103 68-69 -0.1598503481156186 1103 70-71 0.0085948991135254 1103 72-73 -0.08193817985796414 1103 74-75 -0.09028777618487993 1103 76-77 -0.030195757948774826 1103 78-79 -9.304085890207148E-4 1103 80-81 0.058190944784065834 1103 82-83 0.019254788399436507 1103 84-85 -0.07576372952838284 1103 86-87 -0.20618405319273592 1103 88-89 -0.05246175993256941 1103 90-91 0.056305317222367535 1103 92-93 -0.09260017374916885 1103 94-95 -0.20995063593014862 1103 96-97 -0.13615639325394824 1103 98-99 -0.13184549336648388 1103 100-101 -0.06985907429078964 1104 1 0.11634609864240986 1104 2 0.13724421820783306 1104 3 0.15246650850638588 1104 4 0.07278968345905668 1104 5 0.09733619057382725 1104 6 0.11092316073503383 1104 7 0.11997374198774935 1104 8 0.09949656086816105 1104 9 0.14843111652281493 1104 10-11 0.09105739150888326 1104 12-13 0.0767820503554475 1104 14-15 0.15725553646856838 1104 16-17 0.1814792344785161 1104 18-19 0.20745044608273133 1104 20-21 -0.01777565784791335 1104 22-23 0.11956702308557254 1104 24-25 0.07773701437746183 1104 26-27 0.09744253848234763 1104 28-29 0.04630037262315767 1104 30-31 0.09523449153429198 1104 32-33 -0.027571308644738224 1104 34-35 0.17803616046470694 1104 36-37 0.1231709696075427 1104 38-39 0.029287769597672764 1104 40-41 0.07179289325266325 1104 42-43 0.06072356037770987 1104 44-45 0.1870582136752219 1104 46-47 0.26713196867371636 1104 48-49 0.2710645743684026 1104 50-51 0.2355062353714814 1104 52-53 0.32160137439144165 1104 54-55 0.2672419258670615 1104 56-57 0.24983857357700145 1104 58-59 0.2774248422658019 1104 60-61 0.303344755777573 1104 62-63 0.36873429235998145 1104 64-65 0.17778054680900368 1104 66-67 0.2845518306347046 1104 68-69 0.2165506837295439 1104 70-71 0.34434559911923657 1104 72-73 0.35335006739303054 1104 74-75 0.2235576429614028 1104 76-77 0.2612168250110827 1104 78-79 0.3606960128511574 1104 80-81 0.41390915472863554 1104 82-83 0.524841568699685 1104 84-85 0.428937329255767 1104 86-87 0.4380834510317868 1104 88-89 0.304413602315293 1104 90-91 0.2790581560210512 1104 92-93 0.17799387191650595 1104 94-95 0.19302204139782475 1104 96-97 0.10171289547493245 1104 98-99 0.2325697263496025 1104 100-101 0.16319282817372738 1105 1 0.06601867148825846 1105 2 0.11461129943712933 1105 3 0.04061489456709211 1105 4 0.05138052739437171 1105 5 0.13100081714033252 1105 6 -0.004143246606950868 1105 7 0.11620877580106992 1105 8 0.1621225801747599 1105 9 0.15676846463052385 1105 10-11 0.07055007272390412 1105 12-13 0.16923040846141646 1105 14-15 0.15398721274095806 1105 16-17 0.07506689633340358 1105 18-19 0.062047957517407326 1105 20-21 0.09326184906642254 1105 22-23 0.10433838911998805 1105 24-25 0.14002846915325762 1105 26-27 0.019688998046134998 1105 28-29 0.018741920482248986 1105 30-31 -0.01798958045804966 1105 32-33 0.19369777640304875 1105 34-35 0.039325705992247606 1105 36-37 0.1284035588693584 1105 38-39 0.18166853079890188 1105 40-41 0.18199250188650495 1105 42-43 -0.0016623159434416834 1105 44-45 -0.027436311362102117 1105 46-47 0.14063386305243597 1105 48-49 0.20699918410259244 1105 50-51 0.27077900211606476 1105 52-53 0.35415152933199323 1105 54-55 0.19684062686451398 1105 56-57 0.1634116335488116 1105 58-59 0.20096123396503884 1105 60-61 0.10296364960314719 1105 62-63 0.11196642364013343 1105 64-65 0.17157548997764138 1105 66-67 0.1579972207815601 1105 68-69 0.15140130438391708 1105 70-71 0.2167241001511826 1105 72-73 0.30171114727811954 1105 74-75 0.2125303960260041 1105 76-77 0.20970280898767868 1105 78-79 0.28831483530296254 1105 80-81 0.18858960842788264 1105 82-83 0.17392575405735045 1105 84-85 0.21713047261592777 1105 86-87 0.2270200711270718 1105 88-89 0.38948964511575923 1105 90-91 0.24076464330164526 1105 92-93 0.252691722971349 1105 94-95 0.1649379512051823 1105 96-97 0.20950363948697515 1105 98-99 0.08537768928282219 1105 100-101 0.09044883397879744 1106 1 0.20465853485344354 1106 2 0.14175767352182334 1106 3 0.15690536518006581 1106 4 0.10214923750173455 1106 5 0.09338863959492016 1106 6 -0.011669488875625689 1106 7 0.17997385602388505 1106 8 0.08921477757102991 1106 9 0.17206653158584828 1106 10-11 0.18029755921803314 1106 12-13 0.19198183216629872 1106 14-15 0.2031512808299567 1106 16-17 0.14252639032220316 1106 18-19 0.07508703761858726 1106 20-21 0.16884947115546822 1106 22-23 0.1357204796159479 1106 24-25 0.05271463478536731 1106 26-27 0.06599683583917937 1106 28-29 0.0520993478183982 1106 30-31 0.06769523934527655 1106 32-33 -0.00982110860635288 1106 34-35 -0.0330147965835863 1106 36-37 0.09809014649470527 1106 38-39 0.10632416993294669 1106 40-41 0.028327544083509792 1106 42-43 -0.04586482998318786 1106 44-45 0.10115811693456322 1106 46-47 0.11437581024711108 1106 48-49 0.1724152373935155 1106 50-51 0.24112132729411684 1106 52-53 0.20693067274608978 1106 54-55 0.24563492860963976 1106 56-57 0.1433822750760072 1106 58-59 0.18380830018362104 1106 60-61 0.17976688144919706 1106 62-63 0.19841538079690935 1106 64-65 0.37715420332074956 1106 66-67 0.3514226219884833 1106 68-69 0.21282360261753297 1106 70-71 0.16003587886888937 1106 72-73 0.28250511662813693 1106 74-75 0.31488348412046463 1106 76-77 0.16382996311454434 1106 78-79 0.1841112906526874 1106 80-81 0.24686854455927687 1106 82-83 0.25969970774394824 1106 84-85 0.20736915180032867 1106 86-87 0.11697407897177925 1106 88-89 0.2910138809435807 1106 90-91 0.2956927025088305 1106 92-93 0.21678492730195842 1106 94-95 0.27684763072708307 1106 96-97 0.040774359052740294 1106 98-99 0.133565927045602 1106 100-101 0.26858336393451054 1107 1 0.24121641137399763 1107 2 0.15162509530547652 1107 3 0.1061496269293869 1107 4 0.041376545109500285 1107 5 0.022669260415568715 1107 6 0.0910789501393765 1107 7 0.24615327857191005 1107 8 0.1738629459281853 1107 9 0.19683534556288862 1107 10-11 0.10884101550637837 1107 12-13 0.07624271600108301 1107 14-15 0.15801307479836169 1107 16-17 0.15867074346193277 1107 18-19 0.04658677004564282 1107 20-21 0.1402266641717489 1107 22-23 0.15500458381215054 1107 24-25 0.12778394881745925 1107 26-27 0.10194865642777273 1107 28-29 0.0305546914150554 1107 30-31 0.12507903149443678 1107 32-33 0.26399449014649434 1107 34-35 0.21574427765258974 1107 36-37 0.32428504499907973 1107 38-39 0.19470079266966422 1107 40-41 0.15053982604583638 1107 42-43 0.169436486287168 1107 44-45 -0.03669143637500838 1107 46-47 0.2682024032701662 1107 48-49 0.1648486430079643 1107 50-51 0.2990921089284555 1107 52-53 0.17723885936418782 1107 54-55 0.28811540049776596 1107 56-57 0.24376797429831498 1107 58-59 0.26619763017153986 1107 60-61 0.29664012985329435 1107 62-63 0.19209817424987818 1107 64-65 0.11949538303250762 1107 66-67 0.24097690844705966 1107 68-69 0.3287651658862174 1107 70-71 0.3338944521073941 1107 72-73 0.25619577659712434 1107 74-75 0.27948373356957035 1107 76-77 0.11006322588060158 1107 78-79 0.24665973335244473 1107 80-81 0.30630165839281887 1107 82-83 0.210918096539217 1107 84-85 0.3474175591521842 1107 86-87 0.17486152523760978 1107 88-89 0.0861890416051807 1107 90-91 0.30951202028994373 1107 92-93 0.31581026768088094 1107 94-95 0.3419565472687438 1107 96-97 0.1523732712798207 1107 98-99 0.2546247960925285 1107 100-101 0.32990684098600553 1108 1 0.08141638189451328 1108 2 0.13981656168926548 1108 3 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-0.6413197668457755 2316 60-61 -0.8124351914243917 2316 62-63 -0.81220025898741 2316 64-65 -0.6749207783693905 2316 66-67 -0.8080146243170745 2316 68-69 -0.8222898505238376 2316 70-71 -0.9683422651741367 2316 72-73 -1.0627677088164873 2316 74-75 -0.8275675824757585 2316 76-77 -0.7859877543035836 2316 78-79 -0.8277335070003815 2316 80-81 -1.1260945045410722 2316 82-83 -1.5346052281324347 2316 84-85 -1.3982177192295566 2316 86-87 -1.053202912960998 2316 88-89 -0.9096863480231931 2316 90-91 -0.7371667845158214 2316 92-93 -0.7563777791982673 2316 94-95 -0.8933171206294119 2316 96-97 -0.6639239481435624 2316 98-99 -0.642825605467749 2316 100-101 -0.7946961652657265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 2.0 13 20.0 14 58.0 15 245.0 16 479.0 17 968.0 18 1616.0 19 2396.0 20 3306.0 21 4542.0 22 5737.0 23 6343.0 24 6706.0 25 7330.0 26 8579.0 27 10940.0 28 13583.0 29 16973.0 30 21337.0 31 27065.0 32 34322.0 33 45663.0 34 65008.0 35 98988.0 36 177363.0 37 362977.0 38 501687.0 39 152928.0 40 214.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.21092156405078 18.484178853042955 13.223000537858107 24.081899045048154 2 15.631963837968232 19.937933679695945 40.13541742611794 24.294685056217887 3 19.109632984483053 24.047783274966207 29.76837423258432 27.074209507966412 4 12.34815246886967 16.153153610103725 37.842750627678726 33.655943293347875 5 13.060931572434221 38.00184610390927 35.650726269450026 13.286496054206479 6 32.69017659708275 36.96778700829733 17.298031667782443 13.044004726837482 7 28.64713296005518 31.91819832430568 21.35838252895949 18.07628618667965 8 25.77850810459903 36.5784061631469 19.70367242813381 17.93941330412026 9 25.864600450368208 15.722250116649422 19.575484855861887 38.837664577120485 10-11 24.94731757044611 26.09498306047512 28.75103970137748 20.206659667701295 12-13 25.282684661108068 23.321452843202888 28.728280384638794 22.66758211105025 14-15 22.29592056681476 25.269720092102325 25.869798957255597 26.564560383827317 16-17 22.537714533503742 28.409047684255373 26.539106719006757 22.51413106323413 18-19 22.487346073478992 27.73986671535512 27.928629572150204 21.844157639015684 20-21 23.003171089201306 26.80090225792709 28.025245724545066 22.170680928326536 22-23 22.86574232568755 26.676683530500718 27.749914412872307 22.70765973093943 24-25 22.684825938774267 26.761374586655307 27.85829123810683 22.695508236463596 26-27 22.686918020814314 26.931308705089975 27.708168502627146 22.67360477146857 28-29 22.548768455414052 27.160148987599587 27.71032387174993 22.58075868523643 30-31 22.86711861161194 26.913920688449345 27.734123724446526 22.48483697549219 32-33 22.59584905564685 26.995244000162295 27.75118297729901 22.65772396689185 34-35 22.78844739618201 26.875107773923272 27.832869271498993 22.503575558395717 36-37 22.762930334935998 26.845786927149902 27.784434402450653 22.606848335463454 38-39 22.709772432191183 27.018985961495545 27.693048455155907 22.578193151157365 40-41 22.807783305946078 26.989982096849452 27.567935609518596 22.634298987685877 42-43 22.72628720102246 26.883729687785284 27.651238512567705 22.738744598624553 44-45 22.726350597447915 26.972991854827256 27.489324041953218 22.81133350577161 46-47 22.683779897754246 26.960883137565173 27.49937237538799 22.855964589292597 48-49 22.725024241843865 26.99656713928485 27.40241587652102 22.875992742350263 50-51 22.612787657625013 27.130380696683776 27.51951955379167 22.73731209189954 52-53 22.759697117237742 27.1964959527722 27.303065343963645 22.740741586026413 54-55 22.71530223841802 27.065344460758585 27.380553886103655 22.838799414719748 56-57 22.734655529182643 27.09794620940093 27.283032073519564 22.884366187896862 58-59 22.835994715273973 26.98842888442578 27.265376169030088 22.91020023127016 60-61 22.77092550251985 27.126388104798128 27.405300767001894 22.697385625680123 62-63 22.681148946097824 27.20445220416692 27.32097483415495 22.793424015580303 64-65 22.60063548576877 27.135381798632668 27.366556864057777 22.89742585154079 66-67 22.610874008479907 27.12311459030694 27.184831010488303 23.08118039072485 68-69 22.586498082892096 27.099182439697323 27.181946473130058 23.132373004280527 70-71 22.73573326841539 26.915015823747794 27.19215329762847 23.157097610208346 72-73 22.669896080579395 26.948837816728542 27.203945032763272 23.177321069928794 74-75 22.52012202543972 27.192945752946667 27.146507871300184 23.14042435031343 76-77 22.49958158359199 27.05173021524354 27.144796167812874 23.30389203335159 78-79 22.577862870057643 26.939546619457357 27.138960518203533 23.343629992281468 80-81 22.590924725660546 27.03250544082146 27.026862520388224 23.349707313129777 82-83 22.50937914572506 26.996311741089613 27.1281507510449 23.36615836214043 84-85 22.59315470756497 26.892161412370925 27.210189580670686 23.304494299393422 86-87 22.518917493356057 27.01232933682267 27.06916423224393 23.39958893757734 88-89 22.508583876006735 26.800838861501635 27.103620189479237 23.586957073012396 90-91 22.547445884811232 26.75861684214797 27.06431440569655 23.629622867344246 92-93 22.64643940316069 26.783721826628526 27.05163512060536 23.51820364960542 94-95 22.499232903251983 26.77227877183373 27.167206804211553 23.561281520702735 96-97 22.32533650822632 26.71018197309963 27.306678940214635 23.657802578459417 98-99 22.48829701986083 26.633789280425212 27.069988385774856 23.807925313939098 100-101 22.46078297121295 26.6813682977299 26.976225072525512 23.881623658531637 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 34.0 1 29.5 2 62.0 3 130.0 4 115.0 5 79.0 6 124.0 7 254.0 8 433.0 9 534.5 10 484.0 11 415.5 12 461.0 13 520.0 14 555.0 15 622.0 16 702.5 17 766.5 18 844.0 19 935.5 20 1069.0 21 1232.0 22 1481.5 23 1908.5 24 2389.5 25 2991.0 26 3968.5 27 5605.5 28 7959.5 29 11495.5 30 15724.5 31 19535.5 32 23382.5 33 27618.5 34 32093.0 35 36300.0 36 39737.5 37 41665.5 38 43589.0 39 46182.5 40 48942.0 41 51086.5 42 52393.0 43 55041.0 44 57775.0 45 59159.5 46 60857.5 47 63697.5 48 66297.0 49 66097.0 50 64248.0 51 62851.5 52 61237.5 53 59451.5 54 57184.0 55 53328.5 56 47588.0 57 42687.5 58 38834.0 59 33463.0 60 26842.0 61 20642.5 62 15539.0 63 11244.5 64 7975.0 65 5662.0 66 3901.5 67 2736.0 68 1904.0 69 1258.5 70 896.5 71 713.0 72 555.5 73 404.5 74 310.0 75 200.0 76 126.0 77 79.5 78 56.5 79 41.5 80 21.5 81 13.0 82 7.0 83 4.0 84 2.5 85 2.0 86 1.5 87 2.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.047674111942872216 2 0.0019652891891343595 3 2.5358570182378834E-4 4 0.008621913862008805 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0022822713164140957 24-25 0.0 26-27 0.0 28-29 0.021047613251374435 30-31 0.0168951473840099 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03531180897896253 50-51 0.011126072667518715 52-53 0.0 54-55 7.607571054713651E-4 56-57 0.0 58-59 0.0 60-61 3.169821272797354E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.2188748909581483E-4 80-81 0.011316261943886555 82-83 0.0046596372710121115 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1577376.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.7773640562112 #Duplication Level Percentage of deduplicated Percentage of total 1 68.68125962667904 36.934971027830784 2 16.091165637273853 17.306809451289432 3 6.367138561958405 10.272237853283345 4 3.104033822421755 6.677070276446702 5 1.7314581195052474 4.655662682035825 6 1.0644822333231039 3.434702915567118 7 0.7096973341795414 2.6715956336927036 8 0.48855423890572836 2.1018527333470827 9 0.35178859700157755 1.702643710659984 >10 1.3628760396482913 11.67670845568307 >50 0.03770388405482173 1.3538558781710983 >100 0.009367655432263677 0.8310524807450778 >500 0.0 0.0 >1k 4.742496163891846E-4 0.38083690124769487 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1956 0.1240034081918325 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1808 0.11462073722435233 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.33964254559471E-5 0.0 10-11 0.0 0.0 0.0 6.33964254559471E-5 0.0 12-13 0.0 0.0 0.0 2.852839145517619E-4 0.0 14-15 0.0 0.0 0.0 4.4377497819162966E-4 0.0 16-17 0.0 0.0 0.0 6.656624672874444E-4 0.0 18-19 0.0 0.0 0.0 8.241535309273122E-4 0.0 20-21 0.0 0.0 0.0 0.001806798125494492 0.0 22-23 0.0 0.0 0.0 0.004184164080092508 0.0 24-25 0.0 0.0 0.0 0.006403038971050656 0.0 26-27 0.0 0.0 0.0 0.0076709674801695975 0.0 28-29 0.0 0.0 0.0 0.010301919136591401 0.0 30-31 0.0 0.0 0.0 0.016039295640354615 0.0 32-33 0.0 0.0 0.0 0.03030349136794271 0.0 34-35 0.0 0.0 0.0 0.05049525287566186 0.0 36-37 0.0 0.0 0.0 0.08542668330188871 0.0 38-39 0.0 0.0 0.0 0.1383943967703325 0.0 40-41 0.0 0.0 0.0 0.1996353437607774 0.0 42-43 0.0 0.0 0.0 0.2522543768892135 0.0 44-45 0.0 0.0 0.0 0.31568250055788855 0.0 46-47 0.0 0.0 0.0 0.38938084515042704 0.0 48-49 0.0 0.0 0.0 0.46761203416306574 0.0 50-51 0.0 0.0 0.0 0.5466990749193598 0.0 52-53 0.0 0.0 0.0 0.6288608423102672 0.0 54-55 0.0 0.0 0.0 0.7135267685066845 0.0 56-57 0.0 0.0 0.0 0.8153731260016635 0.0 58-59 0.0 0.0 0.0 0.9271093258677703 0.0 60-61 0.0 0.0 0.0 1.0443927129612725 0.0 62-63 0.0 0.0 0.0 1.1620881768202382 0.0 64-65 0.0 0.0 0.0 1.2873912117339175 0.0 66-67 0.0 0.0 0.0 1.4292407136915992 0.0 68-69 0.0 0.0 0.0 1.5941031180897896 0.0 70-71 0.0 0.0 0.0 1.7699648023045869 0.0 72-73 0.0 0.0 0.0 1.951880845150427 0.0 74-75 0.0 0.0 0.0 2.136554632503601 0.0 76-77 0.0 0.0 0.0 2.332893362140669 0.0 78-79 0.0 0.0 0.0 2.565621639989451 0.0 80-81 0.0 0.0 0.0 2.8262760432515774 0.0 82-83 0.0 0.0 0.0 3.098785578073966 0.0 84-85 0.0 0.0 0.0 3.374084555616416 0.0 86-87 0.0 0.0 0.0 3.656135252469925 0.0 88-89 0.0 0.0 0.0 3.981739293611669 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4240 0.0 28.350626 1 TACATGG 4300 0.0 26.952042 2 ACATGGG 4290 0.0 26.350565 3 CGCGAAA 55 9.489321E-5 25.906057 72-73 GTATCAA 4200 0.0 24.774378 1 GGTATCA 3160 0.0 24.056967 1 CATGGGG 3100 0.0 23.135853 4 CATACCG 125 2.2582103E-4 22.797329 5 CAACGCA 4495 0.0 22.505705 5 TCAACGC 4485 0.0 22.451414 4 CCTAGAC 620 0.0 22.216549 3 ATCAACG 4585 0.0 22.065334 3 AACGCAG 4700 0.0 21.625126 6 GCGCGAA 55 0.002880424 21.58838 72-73 GTATTAG 450 0.0 21.11667 1 TATCAAC 5005 0.0 20.118797 2 GTATAGA 540 0.0 19.356949 1 GTCTACG 175 9.919687E-5 19.005005 1 ACGCAGA 5305 0.0 18.97987 7 ATGGGGG 1780 0.0 18.94441 5 >>END_MODULE