FastQCFastQC Report
Fri 10 Feb 2017
SRR2031611_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031611_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817304
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG554.9519596E-543.2002565
GGTATCA22800.029.8636931
GTACATG41950.028.148941
TACATGG41700.026.3539892
ACATGGG40750.026.237473
GTATCAA32250.023.6228641
TCAACGC31400.023.1547874
ATCAACG32300.022.5089873
CAACGCA32350.022.4748175
CATGGGG25050.022.1951054
AACGCAG33350.021.9349296
GTATATA14100.021.2747141
GTAATAC4250.021.2437763
ATACCGT1805.169639E-621.1119756
TATCAAC37300.019.5143012
ACGCAGA37500.019.5058527
CGCAGAG38050.018.8504498
CCGTAGG1350.00927259917.6351261
GTCCTAC3902.910383E-1017.0925061
GTATAGT7400.016.7295361
Produced by FastQC (version 0.11.5)