##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031611_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1817304 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08739924635614 33.0 31.0 34.0 30.0 34.0 2 32.33621947676338 34.0 31.0 34.0 31.0 34.0 3 32.48529800187531 34.0 31.0 34.0 31.0 34.0 4 35.56641156350286 37.0 35.0 37.0 33.0 37.0 5 35.2907834902691 37.0 35.0 37.0 32.0 37.0 6 35.50144995003588 37.0 35.0 37.0 33.0 37.0 7 35.646493377002415 37.0 35.0 37.0 33.0 37.0 8 35.72038029960865 37.0 36.0 37.0 35.0 37.0 9 37.67289237243741 39.0 38.0 39.0 35.0 39.0 10-11 37.63096515497682 39.0 38.0 39.0 35.0 39.0 12-13 37.65552763874399 39.0 38.0 39.0 35.0 39.0 14-15 39.13465496141537 41.0 39.0 41.0 36.0 41.0 16-17 39.09297591377117 41.0 39.0 41.0 36.0 41.0 18-19 39.014076621192714 41.0 39.0 41.0 36.0 41.0 20-21 39.01771635345545 41.0 39.0 41.0 36.0 41.0 22-23 38.96328985134023 41.0 39.0 41.0 35.5 41.0 24-25 38.90260875450667 41.0 39.0 41.0 35.0 41.0 26-27 38.818321260504575 41.0 39.0 41.0 35.0 41.0 28-29 38.72508617160365 40.5 38.5 41.0 35.0 41.0 30-31 38.61563640425598 40.0 38.0 41.0 34.0 41.0 32-33 38.49457933290192 40.0 38.0 41.0 34.0 41.0 34-35 38.39256860712352 40.0 38.0 41.0 34.0 41.0 36-37 38.27975176415173 40.0 38.0 41.0 34.0 41.0 38-39 38.12825207009945 40.0 38.0 41.0 33.0 41.0 40-41 38.00253204747252 40.0 38.0 41.0 33.0 41.0 42-43 37.85792690711075 40.0 38.0 41.0 33.0 41.0 44-45 37.682671418760975 40.0 38.0 41.0 32.5 41.0 46-47 37.53935747678979 40.0 37.5 41.0 32.0 41.0 48-49 37.69504744390592 40.0 38.0 41.0 32.5 41.0 50-51 37.47641396266117 39.5 37.5 40.5 32.5 41.0 52-53 37.60790104462434 40.0 37.5 40.5 32.5 41.0 54-55 37.890626994713045 40.0 38.0 41.0 33.0 41.0 56-57 37.803848448030706 40.0 37.5 41.0 33.0 41.0 58-59 37.63548531230879 40.0 37.0 41.0 32.0 41.0 60-61 37.38175946346897 40.0 36.5 41.0 32.0 41.0 62-63 37.09278166999027 39.5 36.0 41.0 31.0 41.0 64-65 36.84357735414658 39.0 35.5 41.0 31.0 41.0 66-67 36.64951983817787 39.0 35.0 41.0 31.0 41.0 68-69 36.41891615271854 38.5 35.0 41.0 31.0 41.0 70-71 36.12958398814948 37.5 35.0 40.5 30.5 41.0 72-73 35.77317966614281 37.0 35.0 40.0 30.0 41.0 74-75 35.34355781971536 36.5 35.0 39.0 29.5 41.0 76-77 34.932391058403 36.0 34.5 39.0 29.0 41.0 78-79 34.55410239563661 36.0 34.0 38.5 29.0 39.5 80-81 34.19382778005221 35.0 34.0 37.0 29.0 39.0 82-83 33.83560840673877 35.0 34.0 37.0 28.5 39.0 84-85 33.53314635305926 35.0 34.0 36.5 28.0 38.0 86-87 33.25114565312133 35.0 34.0 36.0 27.5 37.0 88-89 32.97864639047732 35.0 34.0 36.0 27.0 37.0 90-91 32.761936362876 35.0 34.0 35.0 26.0 36.5 92-93 32.56902477516145 35.0 33.5 35.0 26.0 36.0 94-95 32.3966141603166 35.0 33.0 35.0 25.5 36.0 96-97 32.27522665442876 35.0 33.0 35.0 25.0 36.0 98-99 32.16567673872946 35.0 33.0 35.0 25.0 35.5 100-101 31.71741436765671 34.5 32.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06535085462584789 1101 2 0.1280644356691525 1101 3 0.12410530355700189 1101 4 0.13823342478212908 1101 5 0.09051266060095742 1101 6 0.17652905279741304 1101 7 0.11961614502649809 1101 8 0.11544194645113492 1101 9 0.17794100538991842 1101 10-11 0.15842357075082703 1101 12-13 0.19074924136847926 1101 14-15 0.2933902522216627 1101 16-17 0.2063199521929704 1101 18-19 0.3327845427612459 1101 20-21 0.06675921890822423 1101 22-23 0.17840840335614416 1101 24-25 0.1359873323559384 1101 26-27 0.24087608200168376 1101 28-29 0.2648389877820705 1101 30-31 0.10780104653071731 1101 32-33 0.11958806267789868 1101 34-35 0.1458748704012578 1101 36-37 0.07990208751855477 1101 38-39 0.0664085834031809 1101 40-41 0.037954377387912075 1101 42-43 -0.00322177866873119 1101 44-45 -0.060170220664510055 1101 46-47 0.14510179653766642 1101 48-49 0.0183660649030557 1101 50-51 -0.00785193835866238 1101 52-53 0.060111896113895114 1101 54-55 0.055581603780339606 1101 56-57 0.029402212497984692 1101 58-59 0.11300562634824729 1101 60-61 -0.04040745264475731 1101 62-63 -0.19849749743001865 1101 64-65 -0.0704089543898263 1101 66-67 -0.026410720924936015 1101 68-69 0.027315780116552446 1101 70-71 0.049566803261285486 1101 72-73 0.0777096804060804 1101 74-75 -0.13447505920988334 1101 76-77 -0.08225095950641759 1101 78-79 0.03439050988354353 1101 80-81 -0.0721267412160671 1101 82-83 0.07849180767979647 1101 84-85 0.06792243773592332 1101 86-87 0.017381384014953483 1101 88-89 0.08891278810011727 1101 90-91 -0.08704594416384026 1101 92-93 -0.17842947331538994 1101 94-95 -0.20827519830736207 1101 96-97 0.0244332548882511 1101 98-99 -0.043184627699098144 1101 100-101 -0.025068709571730352 1102 1 -0.011357834402176081 1102 2 0.06468543904784951 1102 3 0.04337035651298038 1102 4 0.05834282595806428 1102 5 0.136304247893257 1102 6 0.1480492046760702 1102 7 0.08918059918007515 1102 8 0.1558766037380792 1102 9 0.2336640869141604 1102 10-11 0.2742208578270535 1102 12-13 0.29149953231753045 1102 14-15 0.30467365718156003 1102 16-17 0.3141951897742672 1102 18-19 0.3785759133317299 1102 20-21 0.2606658681504612 1102 22-23 0.12904719092757944 1102 24-25 0.3088812838665973 1102 26-27 0.31322216074539 1102 28-29 0.2598764914934364 1102 30-31 0.30739577672287766 1102 32-33 0.32185930737634294 1102 34-35 0.1916844456048139 1102 36-37 0.17713151574137243 1102 38-39 0.19862558262948227 1102 40-41 0.30737622858237756 1102 42-43 0.15646536170849146 1102 44-45 -0.21567137904265365 1102 46-47 -0.584861512457806 1102 48-49 -0.12350415773527601 1102 50-51 0.14736336393577432 1102 52-53 0.14886099942735598 1102 54-55 0.003956731917114098 1102 56-57 -0.08341038164055448 1102 58-59 -0.05973358578123111 1102 60-61 -0.17114229132786818 1102 62-63 -0.4065703853133158 1102 64-65 -0.11168405890391853 1102 66-67 -0.06212561036430486 1102 68-69 0.11267903839911497 1102 70-71 0.17946227832635486 1102 72-73 0.015704483416783432 1102 74-75 0.028339810507794994 1102 76-77 -0.012916230484542268 1102 78-79 0.16597143056118568 1102 80-81 0.1075373833036295 1102 82-83 -0.06617412646710363 1102 84-85 -0.008778882750142714 1102 86-87 0.03465519686699281 1102 88-89 -0.1313875450013242 1102 90-91 -0.02585237039931343 1102 92-93 -0.10670451931459723 1102 94-95 -0.03689374839502335 1102 96-97 -0.1075759081104053 1102 98-99 -0.17022912719088623 1102 100-101 -0.03144054787124517 1103 1 0.13108672126369214 1103 2 0.12642372045809935 1103 3 0.05928532135515496 1103 4 0.3075555697449346 1103 5 0.07722269980047969 1103 6 0.16098457500237373 1103 7 0.22814342296432244 1103 8 0.2070412834614075 1103 9 0.3083225596617254 1103 10-11 0.24979540307275272 1103 12-13 0.3167114227990737 1103 14-15 0.3872229478715141 1103 16-17 0.2894536428301677 1103 18-19 0.306378077773509 1103 20-21 0.12884537405385288 1103 22-23 0.1875412711218658 1103 24-25 0.4022979101927149 1103 26-27 0.22240462860285248 1103 28-29 0.1548641431807667 1103 30-31 0.31356542038586355 1103 32-33 0.2754511894889333 1103 34-35 0.40576357326420975 1103 36-37 0.30562842661199596 1103 38-39 0.3385180169290791 1103 40-41 0.2217412955750504 1103 42-43 0.1201680914650538 1103 44-45 0.2571213087870845 1103 46-47 0.5167964844079407 1103 48-49 0.24549891088195608 1103 50-51 0.4075628240560647 1103 52-53 0.3034021837166847 1103 54-55 0.31818088045788073 1103 56-57 0.37764443502187106 1103 58-59 0.3491586351920333 1103 60-61 0.43659530087423803 1103 62-63 0.48533613672903897 1103 64-65 0.6006519464784645 1103 66-67 0.3676010037365671 1103 68-69 0.3454787824075183 1103 70-71 0.46535340805338166 1103 72-73 0.5402288067210463 1103 74-75 0.4094140726858555 1103 76-77 -0.16248260369075496 1103 78-79 0.1647238817627894 1103 80-81 0.41043144216528304 1103 82-83 0.12381635589655104 1103 84-85 0.20115597067005098 1103 86-87 0.24888919123845454 1103 88-89 0.3223887413154287 1103 90-91 0.2702476550357531 1103 92-93 0.14948522625272886 1103 94-95 -0.09256371614993242 1103 96-97 0.15000599689373928 1103 98-99 0.2385154063218593 1103 100-101 0.1593246782956932 1104 1 0.16974845548808304 1104 2 0.1749534374933006 1104 3 0.2289030880617915 1104 4 0.116713119758721 1104 5 0.04561107386054175 1104 6 0.053236891732090896 1104 7 0.15835416713725436 1104 8 0.2350465257304606 1104 9 0.28952227090316995 1104 10-11 0.3669223174149394 1104 12-13 0.25376159304888546 1104 14-15 0.26391537663459275 1104 16-17 0.19682356980399618 1104 18-19 0.27194005707087854 1104 20-21 0.26301431020165467 1104 22-23 0.2791128984250406 1104 24-25 0.2306356740489406 1104 26-27 0.25112557072412045 1104 28-29 0.25815187001268924 1104 30-31 0.13713843571299122 1104 32-33 0.0529525759637508 1104 34-35 0.07491040327155929 1104 36-37 0.07030995566909581 1104 38-39 0.15609820784433737 1104 40-41 0.06381716595591769 1104 42-43 0.0031345124390895762 1104 44-45 0.228500285801033 1104 46-47 0.18294922591016416 1104 48-49 0.2327282384649081 1104 50-51 0.06932028545939062 1104 52-53 0.14215322550921883 1104 54-55 0.04383817796284717 1104 56-57 0.008894028374221818 1104 58-59 -0.06428002375375996 1104 60-61 0.11864099412713358 1104 62-63 0.10410087286506808 1104 64-65 0.1716162203529379 1104 66-67 0.07965574393132613 1104 68-69 -0.06157163570371438 1104 70-71 -0.11493936275845584 1104 72-73 -0.18113855178881266 1104 74-75 -0.21148033525066268 1104 76-77 -0.14194031388498018 1104 78-79 -0.1873934744233665 1104 80-81 -0.14174764700596398 1104 82-83 0.048276715109714985 1104 84-85 -0.11188215971125004 1104 86-87 -0.09826125444736533 1104 88-89 -0.13206284979997207 1104 90-91 -0.015289088870737544 1104 92-93 0.011242842569444633 1104 94-95 -0.22813461845844785 1104 96-97 -0.09274444383484592 1104 98-99 0.01436386694752656 1104 100-101 -0.04668108138406879 1105 1 -0.01687829548816211 1105 2 0.09574154100540966 1105 3 0.054142074674956575 1105 4 0.07843271875082536 1105 5 -0.08913572870456932 1105 6 -0.05308453555984727 1105 7 0.1687629969537099 1105 8 0.1839860879416122 1105 9 0.2365265042950213 1105 10-11 0.2165637628585131 1105 12-13 0.22309898363153025 1105 14-15 0.18051487029942592 1105 16-17 0.20483768210531395 1105 18-19 0.1596488158332221 1105 20-21 0.14855860557179312 1105 22-23 0.07957512331428518 1105 24-25 0.11320159315473433 1105 26-27 0.16146976518547973 1105 28-29 0.2039787099558481 1105 30-31 0.14236561480516485 1105 32-33 0.2069955696703829 1105 34-35 0.0452143387138193 1105 36-37 0.0449987953972979 1105 38-39 0.11151436698165895 1105 40-41 0.20981171449363956 1105 42-43 0.0032513188255975933 1105 44-45 0.2866105301316324 1105 46-47 0.277125774471763 1105 48-49 0.11093537611881032 1105 50-51 -0.0823921782671917 1105 52-53 0.056221416555210624 1105 54-55 0.04582724518361658 1105 56-57 0.20848740780316888 1105 58-59 0.17264363189056553 1105 60-61 0.37529895815823977 1105 62-63 0.32050616191795456 1105 64-65 0.44178752643493624 1105 66-67 0.29770863744569454 1105 68-69 0.2567933137224756 1105 70-71 0.13561779997336743 1105 72-73 0.2173238581993573 1105 74-75 0.07815994586088237 1105 76-77 0.04659228442643126 1105 78-79 0.16897833261995032 1105 80-81 0.3364886735854 1105 82-83 0.24751258489559547 1105 84-85 0.23695694154383773 1105 86-87 0.14741056513491202 1105 88-89 0.1215025438636701 1105 90-91 0.22758087016865858 1105 92-93 0.19780652556064382 1105 94-95 0.15497241649539717 1105 96-97 0.2030410473938531 1105 98-99 0.20033157613918462 1105 100-101 0.10481008461982455 1106 1 0.07317587790088709 1106 2 -0.03490811209107392 1106 3 0.01103959703698365 1106 4 0.09569059890520748 1106 5 0.11271644250670931 1106 6 -0.09166151849687054 1106 7 0.018916780396978083 1106 8 0.12523055088251311 1106 9 0.20397935734132489 1106 10-11 0.12880244502420624 1106 12-13 0.12201647788781145 1106 14-15 0.13176380849530034 1106 16-17 0.2531802343688838 1106 18-19 0.23072110260300605 1106 20-21 0.14018371460269208 1106 22-23 0.27151813222488386 1106 24-25 0.33200984985764137 1106 26-27 0.17162781009765382 1106 28-29 0.16362997499926024 1106 30-31 0.1432455226206244 1106 32-33 0.21547165271059043 1106 34-35 0.37362625742950684 1106 36-37 0.31238583796638153 1106 38-39 0.14337971454079224 1106 40-41 0.2243260068047519 1106 42-43 0.10085837576110634 1106 44-45 0.3313657522125979 1106 46-47 0.49326063642298124 1106 48-49 0.23390191432380192 1106 50-51 0.07206397848645452 1106 52-53 0.09024208396679967 1106 54-55 0.16551929348216277 1106 56-57 0.28106350139015746 1106 58-59 0.1681484235489208 1106 60-61 0.19359879596586893 1106 62-63 0.32751585070245426 1106 64-65 0.2472756997897818 1106 66-67 0.15700694169462537 1106 68-69 0.18641957292240363 1106 70-71 0.1806940574630005 1106 72-73 0.16374176577537014 1106 74-75 0.2884580919081188 1106 76-77 0.2127020977769476 1106 78-79 0.16833323316667048 1106 80-81 0.3049447177450517 1106 82-83 0.3669452394344148 1106 84-85 0.2741154539348756 1106 86-87 0.27624553084362446 1106 88-89 0.15937296385352084 1106 90-91 0.1657877894259414 1106 92-93 0.3126810830234845 1106 94-95 0.1322654858361929 1106 96-97 0.22571751353869018 1106 98-99 0.24977886789729808 1106 100-101 0.2043764908475545 1107 1 0.13089992381965487 1107 2 0.16661103518736553 1107 3 0.09576039611005882 1107 4 0.2752482333471633 1107 5 0.1872133455592433 1107 6 0.1812862241364286 1107 7 0.15834188806409344 1107 8 0.18822431478159274 1107 9 0.25348529615806825 1107 10-11 0.2849603811384185 1107 12-13 0.23834486586392956 1107 14-15 0.23424176843244737 1107 16-17 0.353355791144466 1107 18-19 0.2832166316164333 1107 20-21 0.15791860008882708 1107 22-23 0.30038919932268016 1107 24-25 0.31598330162675836 1107 26-27 0.31444296231217805 1107 28-29 0.1884991783346024 1107 30-31 0.30686118827423314 1107 32-33 0.3276526332894818 1107 34-35 0.3711747934682279 1107 36-37 0.329916225434026 1107 38-39 0.39062552316882915 1107 40-41 0.30969630900290923 1107 42-43 0.3788008275734285 1107 44-45 0.34498220504121946 1107 46-47 0.5454258869003894 1107 48-49 0.29971058256102623 1107 50-51 0.15073106676629067 1107 52-53 0.17078767329123679 1107 54-55 0.215253513051735 1107 56-57 0.26063379762587857 1107 58-59 0.21533604821986074 1107 60-61 0.33367457914638976 1107 62-63 0.28346252244276826 1107 64-65 0.5273641246659793 1107 66-67 0.24759118968623994 1107 68-69 0.19421093067871453 1107 70-71 0.18690972532740346 1107 72-73 0.3046154429445167 1107 74-75 0.3921615325998431 1107 76-77 0.28626376992425406 1107 78-79 0.2876024734824867 1107 80-81 0.36772828916812017 1107 82-83 0.36174636720497944 1107 84-85 0.30255357477729916 1107 86-87 0.3139056581515973 1107 88-89 0.20192881727015077 1107 90-91 0.30405206517971095 1107 92-93 0.2603620821931045 1107 94-95 0.2176666050985645 1107 96-97 0.31264424496362153 1107 98-99 0.25324779742972936 1107 100-101 0.32115574587182394 1108 1 0.07481817521859568 1108 2 0.06542692169291797 1108 3 0.13160750287218548 1108 4 -0.04198433013221603 1108 5 0.06264449378512893 1108 6 0.21607312140514523 1108 7 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2316 68-69 -1.5814523309957238 2316 70-71 -1.1985023776504704 2316 72-73 -0.9814274641682701 2316 74-75 -1.0670349540657469 2316 76-77 -0.9677687347350954 2316 78-79 -1.1954123331708288 2316 80-81 -1.1575100168440713 2316 82-83 -1.1979194880649686 2316 84-85 -1.6707684581524447 2316 86-87 -1.2056136043329033 2316 88-89 -1.046720748575801 2316 90-91 -1.1481517641582926 2316 92-93 -1.416860344437822 2316 94-95 -1.335355104107947 2316 96-97 -1.0721057993991927 2316 98-99 -0.7758060865973526 2316 100-101 -1.3744623359101489 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 8.0 7 120.0 8 497.0 9 583.0 10 562.0 11 718.0 12 916.0 13 1266.0 14 1699.0 15 2068.0 16 2569.0 17 3127.0 18 3914.0 19 5019.0 20 6070.0 21 7203.0 22 8186.0 23 8974.0 24 9282.0 25 10605.0 26 12015.0 27 14108.0 28 16593.0 29 19929.0 30 24648.0 31 30773.0 32 38807.0 33 51305.0 34 72301.0 35 108959.0 36 186758.0 37 357583.0 38 558068.0 39 251802.0 40 269.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.537656234194166 20.572473153229048 13.256752252409122 23.633118360167668 2 14.705196949532546 20.352206398029676 42.186849414922115 22.75574723751567 3 18.066246561144098 25.41688030300524 30.879906055142985 25.636967080707674 4 11.678210614549785 16.634424493757443 40.27556580050293 31.411799091189835 5 12.29048615320801 38.691313094094866 36.80057938814538 12.21762136455175 6 30.626697378910656 38.95528016469055 17.995166019232276 12.42285643716652 7 26.65687623482924 33.0769408869602 23.0796200254022 17.186562852808354 8 24.393906484806454 38.29169225477042 20.51743280297509 16.79696845744804 9 25.026253512182457 16.293660073107418 21.18819675167568 37.49188966303445 10-11 23.844328719497117 27.104447691800043 30.032939432958784 19.018284155744055 12-13 24.022219637361143 24.37983088025729 30.06561259461924 21.53233688776233 14-15 21.216378931072466 26.611435105396463 26.790422192303748 25.381763771227323 16-17 21.254946515265875 29.839131379390388 27.65843787182333 21.247484233520407 18-19 21.23619475459161 29.1784545831612 28.884507822204046 20.700842840043148 20-21 21.864398758817345 28.246397538324736 28.915797565507933 20.97340613734999 22-23 21.600321135044 28.16570590280988 28.634944951422547 21.59902801072358 24-25 21.456646769060104 28.298182362444592 28.687605375875474 21.557565492619837 26-27 21.578558127864135 28.420396367366163 28.485245176371155 21.51580032839855 28-29 21.557015226951574 28.321266007228292 28.638136492298482 21.483582273521655 30-31 21.728918221717443 28.314470226225218 28.47616579284479 21.48044575921255 32-33 21.546780285521848 28.40763020386243 28.607101508608356 21.438488002007368 34-35 21.77337968771323 28.36979943916923 28.471818694065494 21.385002179052044 36-37 21.887642353728378 28.10616715750364 28.449918120468563 21.556272368299414 38-39 21.730073779620803 28.39269049096904 28.458529778176906 21.418705951233257 40-41 21.757559549750617 28.26643203338572 28.53262305040874 21.443385366454926 42-43 21.65499002918609 28.18303927136021 28.587236917984004 21.574733781469693 44-45 21.72148355493352 28.381170688163632 28.388003284054154 21.509342472848697 46-47 21.67761604368392 28.246167028797014 28.30921933960769 21.76699758791138 48-49 21.656172853954487 28.162235767264537 28.43286736490794 21.748724013873037 50-51 21.560280759134375 28.409999738457365 28.37542104922645 21.654298453181802 52-53 21.535230432141834 28.368403982156398 28.368816977523693 21.72754860817808 54-55 21.59602035750367 28.240763304506476 28.431363640493952 21.7318526974959 56-57 21.69773667612645 28.29376577108612 28.20341901859738 21.80507853419005 58-59 21.672589455966282 28.195497211368668 28.32889766719946 21.803015665465587 60-61 21.762294438335562 28.221546342244274 28.332746017572084 21.683413201848076 62-63 21.58876579324252 28.31309568099477 28.286647782665014 21.811490743097693 64-65 21.719710197791276 28.28759050809056 28.247092252821517 21.74560704129665 66-67 21.62714424449276 28.230792069772605 28.247675559372322 21.894388126362315 68-69 21.68835114336874 28.06795843477346 28.191187702633506 22.05250271922429 70-71 21.671932768666206 27.969335259494816 28.289790003970914 22.068941967868057 72-73 21.63920415905145 27.98813430242771 28.23901660305585 22.13364493546499 74-75 21.53764894343359 28.076501943297483 28.24832107142837 22.137528041840547 76-77 21.58688771939693 28.0188085630635 28.25392938820056 22.14037432933901 78-79 21.72608986148134 27.924754957672942 28.132167875519187 22.216987305326526 80-81 21.762342740906185 27.853808350087167 28.169930555000995 22.213918354005653 82-83 21.61742019306446 27.99743124137023 28.281046647973774 22.104101917591535 84-85 21.76126974684381 27.98808028730694 28.07560534954943 22.17504461629982 86-87 21.547359117604138 27.994372138229533 28.21893593764197 22.239332806524356 88-89 21.728337193966453 27.802865084573614 28.148352963305577 22.320444758154352 90-91 21.74719530711139 27.888624328713753 28.119456448204357 22.244723915970496 92-93 21.91973247465355 27.734837051841577 28.11303574243575 22.232394731069117 94-95 21.726012957949344 27.696604248071694 28.20564883279551 22.371733961183455 96-97 21.69096629005555 27.616340229976217 28.28838013158808 22.404313348380153 98-99 21.851949754112354 27.635183135356606 28.036285637187685 22.47658147334336 100-101 21.820594530975765 27.494063637802757 28.131129939225417 22.55421189199606 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 29.0 1 19.0 2 26.0 3 43.5 4 41.0 5 44.0 6 76.5 7 141.0 8 212.0 9 273.5 10 289.0 11 269.0 12 293.0 13 334.0 14 387.0 15 451.0 16 478.5 17 532.0 18 636.0 19 743.5 20 868.0 21 984.0 22 1183.0 23 1559.5 24 2006.5 25 2571.0 26 3532.5 27 5264.0 28 8267.5 29 13082.0 30 19831.5 31 28921.5 32 39631.5 33 50798.0 34 63043.5 35 74443.0 36 83269.0 37 87898.0 38 87756.5 39 84644.0 40 78828.5 41 71665.5 42 65874.5 43 61639.5 44 57957.0 45 56206.0 46 55614.0 47 55241.5 48 55639.0 49 55167.0 50 53838.0 51 52647.0 52 51205.0 53 49764.5 54 48122.5 55 45451.0 56 41463.5 57 36941.0 58 33022.5 59 28698.5 60 23435.0 61 18727.0 62 14700.5 63 11058.0 64 8125.5 65 5925.5 66 4118.5 67 2951.0 68 2239.5 69 1617.0 70 1183.0 71 881.0 72 685.0 73 506.5 74 355.5 75 256.0 76 176.0 77 124.0 78 87.5 79 57.0 80 45.5 81 36.5 82 31.5 83 22.0 84 16.0 85 15.0 86 16.5 87 17.0 88 12.0 89 8.5 90 6.5 91 5.5 92 4.5 93 3.5 94 2.5 95 2.5 96 2.0 97 1.5 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4534739372168883 2 0.3540409309614682 3 0.1911622931551353 4 0.21702477956357327 5 0.23975075166290283 6 0.19138239942244117 7 0.18092735172541302 8 0.18213793619559523 9 0.1794416344210985 10-11 0.19372102851256587 12-13 0.18285328156433925 14-15 0.19182261195705286 16-17 0.08284249635724128 18-19 0.035684728586961784 20-21 2.751328341323191E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.1361357263286715 46-47 0.20527660754612329 48-49 0.08537371843125861 50-51 0.0636382245348054 52-53 0.07169961657488236 54-55 0.08047635398370334 56-57 0.11420763944832565 58-59 0.09453564180786483 60-61 0.14034525869089598 62-63 0.23691688347133996 64-65 0.13266905261860426 66-67 0.105706034873637 68-69 0.1094203281344233 70-71 0.0882075866228215 72-73 0.07224988224314699 74-75 0.14334420658293823 76-77 0.21906076253615245 78-79 0.1412256837601194 80-81 0.08531869186443214 82-83 0.09029859616222712 84-85 0.10036845789146999 86-87 0.07368057298063506 88-89 0.09659913806385724 90-91 0.11731664047402086 92-93 0.1676109225534088 94-95 0.1339071503721997 96-97 0.09013351646174772 98-99 0.07890809682914911 100-101 0.06418849020307005 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1817304.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.295678997192255 #Duplication Level Percentage of deduplicated Percentage of total 1 69.43305341844778 37.69914780202943 2 15.732074676362803 17.08367353175304 3 6.188193433647035 10.079764927374967 4 2.994054885036729 6.502569717517185 5 1.7086435460983738 4.638598074979078 6 1.0615564839613294 3.4582758054331446 7 0.6898245385675599 2.621814419731535 8 0.4762052529624255 2.068471004129967 9 0.3262539994621081 1.5942764183710278 >10 1.3383607480048751 11.601944127332308 >50 0.03791040641163687 1.3704798855838762 >100 0.013562810359704974 1.1397425235673728 >500 2.0386711843186636E-4 0.08080445642935208 >1k 1.019335592159331E-4 0.06043730576770714 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.5026566826463816E-5 0.0 5 0.0 0.0 0.0 5.5026566826463816E-5 0.0 6 0.0 0.0 0.0 1.1005313365292763E-4 0.0 7 0.0 0.0 0.0 1.1005313365292763E-4 0.0 8 0.0 0.0 0.0 1.1005313365292763E-4 0.0 9 0.0 0.0 0.0 1.6507970047939145E-4 0.0 10-11 0.0 0.0 0.0 1.9259298389262335E-4 0.0 12-13 0.0 0.0 0.0 2.751328341323191E-4 0.0 14-15 0.0 0.0 0.0 3.576726843720148E-4 0.0 16-17 0.0 0.0 0.0 6.05292235091102E-4 0.0 18-19 0.0 0.0 0.0 9.629649194631167E-4 2.7513283413231908E-5 20-21 0.0 0.0 0.0 0.0016507970047939147 5.5026566826463816E-5 22-23 0.0 0.0 0.0 0.0038793729612656993 1.1005313365292763E-4 24-25 0.0 0.0 0.0 0.006245515334803643 1.1005313365292763E-4 26-27 0.0 0.0 0.0 0.007868799056184326 1.1005313365292763E-4 28-29 0.0 0.0 0.0 0.010124888296069343 1.1005313365292763E-4 30-31 0.0 0.0 0.0 0.01642543019769945 1.1005313365292763E-4 32-33 0.0 0.0 0.0 0.03100747040671236 1.1005313365292763E-4 34-35 0.0 0.0 0.0 0.05442127459137271 1.1005313365292763E-4 36-37 0.0 0.0 0.0 0.09084886183049176 1.1005313365292763E-4 38-39 0.0 0.0 0.0 0.15179078459080042 1.1005313365292763E-4 40-41 0.0 0.0 0.0 0.21639197404506896 1.1005313365292763E-4 42-43 0.0 0.0 0.0 0.2816809955846683 1.1005313365292763E-4 44-45 0.0 0.0 0.0 0.35585680766674155 1.3756641706615954E-4 46-47 0.0 0.0 0.0 0.4480813336678948 1.6507970047939145E-4 48-49 0.0 0.0 0.0 0.5436899935288757 1.6507970047939145E-4 50-51 0.0 0.0 0.0 0.6460118945426852 1.6507970047939145E-4 52-53 0.0 0.0 0.0 0.7468755915355934 1.6507970047939145E-4 54-55 0.0 0.0 0.0 0.8549202554993551 1.6507970047939145E-4 56-57 0.0 0.0 0.0 0.9859109978297522 1.9259298389262335E-4 58-59 0.0 0.0 0.0 1.1312361608184431 2.2010626730585526E-4 60-61 0.0 0.0 0.0 1.2778269348441427 2.2010626730585526E-4 62-63 0.0 0.0 0.0 1.4294251264510507 2.2010626730585526E-4 64-65 0.0 0.0 0.0 1.5891672499482752 2.2010626730585526E-4 66-67 0.0 0.0 0.0 1.7664903615465546 2.2010626730585526E-4 68-69 0.0 0.0 0.0 1.9776823250265227 2.2010626730585526E-4 70-71 0.0 0.0 0.0 2.2101420565849192 2.751328341323191E-4 72-73 0.0 0.0 0.0 2.4389975480161823 3.301594009587829E-4 74-75 0.0 0.0 0.0 2.6706869076390083 3.301594009587829E-4 76-77 0.0 0.0 0.0 2.918636617759054 3.301594009587829E-4 78-79 0.0 0.0 0.0 3.2123959447621315 3.301594009587829E-4 80-81 0.0 0.0 0.0 3.5291838899820833 3.301594009587829E-4 82-83 0.0 0.0 0.0 3.866386691494654 3.301594009587829E-4 84-85 0.0 0.0 0.0 4.191951374123427 3.301594009587829E-4 86-87 0.0 0.0 0.0 4.53572985037176 3.576726843720148E-4 88-89 0.0 0.0 0.0 4.9423211526525 3.851859677852467E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 55 4.9519596E-5 43.200256 5 GGTATCA 2280 0.0 29.863693 1 GTACATG 4195 0.0 28.14894 1 TACATGG 4170 0.0 26.353989 2 ACATGGG 4075 0.0 26.23747 3 GTATCAA 3225 0.0 23.622864 1 TCAACGC 3140 0.0 23.154787 4 ATCAACG 3230 0.0 22.508987 3 CAACGCA 3235 0.0 22.474817 5 CATGGGG 2505 0.0 22.195105 4 AACGCAG 3335 0.0 21.934929 6 GTATATA 1410 0.0 21.274714 1 GTAATAC 425 0.0 21.243776 3 ATACCGT 180 5.169639E-6 21.111975 6 TATCAAC 3730 0.0 19.514301 2 ACGCAGA 3750 0.0 19.505852 7 CGCAGAG 3805 0.0 18.850449 8 CCGTAGG 135 0.009272599 17.635126 1 GTCCTAC 390 2.910383E-10 17.092506 1 GTATAGT 740 0.0 16.729536 1 >>END_MODULE