FastQCFastQC Report
Fri 10 Feb 2017
SRR2031611_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031611_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817304
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG41000.026.2005561
TCAACGC29050.025.8350894
GTATCAA30800.025.7722111
CAACGCA29750.025.2244285
AACGCAG30700.024.4438696
GGTATCA22000.024.4141541
ATCAACG30450.024.3352783
ACATGGG38250.024.2159883
TACATGG41600.023.864482
TATCAAC35750.020.9932672
CATGGGG23000.020.8589534
ACGCAGA36450.020.587847
CGCAGAG37700.019.7792368
GACCGCG1250.00603586418.9981467
GCACCGT2752.8432623E-818.9981466
GAGTACA33250.017.7262121
GTACTAG5900.017.723791
ATATCGA2453.7676327E-617.4472776
GTGCGCG1650.001465213517.2710429
TACACTG17500.017.098335
Produced by FastQC (version 0.11.5)