##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031611_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1817304 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.431940390820685 34.0 31.0 34.0 31.0 34.0 2 32.65742495476816 34.0 31.0 34.0 31.0 34.0 3 32.771427895387895 34.0 31.0 34.0 31.0 34.0 4 36.23040283849042 37.0 37.0 37.0 35.0 37.0 5 35.89464668541972 37.0 35.0 37.0 35.0 37.0 6 35.95702425130853 37.0 36.0 37.0 35.0 37.0 7 35.92490139239225 37.0 36.0 37.0 35.0 37.0 8 36.0397968639259 37.0 36.0 37.0 35.0 37.0 9 37.941308663822895 39.0 38.0 39.0 35.0 39.0 10-11 37.90743458441736 39.0 38.0 39.0 35.0 39.0 12-13 37.925125625652065 39.0 38.0 39.0 35.0 39.0 14-15 39.39675888018735 41.0 39.0 41.0 36.0 41.0 16-17 39.36304630375545 41.0 39.0 41.0 36.0 41.0 18-19 39.356883053138056 41.0 39.0 41.0 36.0 41.0 20-21 39.37308727653711 41.0 39.0 41.0 36.0 41.0 22-23 39.317077109828624 41.0 39.0 41.0 36.0 41.0 24-25 39.25333378455118 41.0 39.0 41.0 36.0 41.0 26-27 39.14695532503092 41.0 39.0 41.0 36.0 41.0 28-29 39.06183692986974 40.5 39.0 41.0 36.0 41.0 30-31 38.97375067682677 40.0 39.0 41.0 35.0 41.0 32-33 38.85091459656722 40.0 38.0 41.0 35.0 41.0 34-35 38.74618335732492 40.0 38.0 41.0 35.0 41.0 36-37 38.62526165132526 40.0 38.0 41.0 35.0 41.0 38-39 38.52061377733169 40.0 38.0 41.0 34.0 41.0 40-41 38.42195389434019 40.0 38.0 41.0 34.0 41.0 42-43 38.29298345241082 40.0 38.0 41.0 34.0 41.0 44-45 38.250702689258375 40.0 38.0 41.0 33.5 41.0 46-47 38.29532345716512 40.0 38.0 41.0 33.5 41.0 48-49 38.43143579720289 40.0 38.0 41.0 34.0 41.0 50-51 38.37477219001334 40.0 38.0 41.0 34.0 41.0 52-53 38.28857307307969 40.0 38.0 41.0 34.0 41.0 54-55 38.19604645122665 40.0 38.0 41.0 34.0 41.0 56-57 38.10107114714984 40.0 37.5 41.0 33.5 41.0 58-59 37.91991268384376 40.0 37.0 41.0 33.0 41.0 60-61 37.65462712897787 40.0 37.0 41.0 33.0 41.0 62-63 37.48280832485924 39.5 36.0 41.0 32.5 41.0 64-65 37.210569613009156 39.0 36.0 41.0 32.0 41.0 66-67 36.95072866179792 39.0 35.0 41.0 32.0 41.0 68-69 36.68333558942257 38.5 35.0 40.5 31.5 41.0 70-71 36.364839344435495 37.5 35.0 40.0 31.0 41.0 72-73 36.00757523232217 37.0 35.0 39.5 31.0 41.0 74-75 35.65104132275063 36.5 35.0 39.0 31.0 41.0 76-77 35.006788077283716 36.0 34.0 39.0 30.0 40.0 78-79 34.9513419328852 36.0 34.0 38.0 30.0 39.5 80-81 34.655905120992415 35.0 34.0 37.0 30.0 39.0 82-83 34.34499263744536 35.0 34.0 37.0 30.0 39.0 84-85 34.04524504430739 35.0 34.0 36.0 30.0 38.0 86-87 33.76721038417348 35.0 34.0 36.0 29.5 37.0 88-89 33.51606858291183 35.0 34.0 36.0 29.0 37.0 90-91 33.338900646232 35.0 34.0 35.0 29.0 36.0 92-93 33.1897272002923 35.0 34.0 35.0 29.0 36.0 94-95 33.05612517223315 35.0 34.0 35.0 29.0 36.0 96-97 32.95214999801904 35.0 34.0 35.0 29.0 36.0 98-99 32.85292031492805 35.0 34.0 35.0 29.0 35.0 100-101 32.41269732526864 34.5 32.5 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16719959688768427 1101 2 0.17530800180121986 1101 3 0.2330248237628112 1101 4 0.14758472294138159 1101 5 0.2737981910331655 1101 6 0.18219998408859084 1101 7 0.33223535722954267 1101 8 0.11400704762827019 1101 9 0.209507379085224 1101 10-11 0.0834722027306114 1101 12-13 0.13784659501975227 1101 14-15 0.15334485025677225 1101 16-17 0.12921890410935788 1101 18-19 0.11588603629536465 1101 20-21 0.05882208352039697 1101 22-23 0.1678451240808485 1101 24-25 -0.045079959668363756 1101 26-27 0.1729817163730445 1101 28-29 0.05523918659204696 1101 30-31 0.06222253388580867 1101 32-33 -0.01329094639302042 1101 34-35 0.0011133563479219788 1101 36-37 -0.017091522098702683 1101 38-39 -0.023665889554010278 1101 40-41 -0.07803812519782127 1101 42-43 0.10034337821554828 1101 44-45 0.18674431843843564 1101 46-47 0.13275172195761797 1101 48-49 0.21641462459532335 1101 50-51 0.2843052143920346 1101 52-53 -0.03396188409600853 1101 54-55 0.15184574773874004 1101 56-57 0.2699577108728377 1101 58-59 0.145307748911641 1101 60-61 0.07723639428356677 1101 62-63 -0.023062570833133123 1101 64-65 0.05501840356061649 1101 66-67 0.06901332854579323 1101 68-69 -0.05182999696882007 1101 70-71 -0.11709413732435081 1101 72-73 -0.09863883100885573 1101 74-75 -0.09344641970267986 1101 76-77 -0.09281250198153401 1101 78-79 0.2755562808287024 1101 80-81 0.2000793893476498 1101 82-83 0.08491223057237818 1101 84-85 0.18844565966049487 1101 86-87 0.04797217786140351 1101 88-89 0.04081648565301066 1101 90-91 0.07634153462952753 1101 92-93 -0.30991763120337623 1101 94-95 -0.12488521894225357 1101 96-97 -0.10820466204945944 1101 98-99 -0.25384234769992275 1101 100-101 -0.2724612218134439 1102 1 0.15492403942497646 1102 2 0.188020470457829 1102 3 0.18952875401306102 1102 4 0.12896398396330966 1102 5 0.1359622451182645 1102 6 0.061122269810304886 1102 7 0.17984437372423656 1102 8 0.14309891817599407 1102 9 0.18377903122069483 1102 10-11 0.2779414618237155 1102 12-13 0.12395667670220689 1102 14-15 0.1346590947319939 1102 16-17 0.20724201099005768 1102 18-19 0.23320536073010345 1102 20-21 0.21619311778195538 1102 22-23 0.15357507568686657 1102 24-25 0.32175444968881806 1102 26-27 0.1495588552548881 1102 28-29 -0.21553002417208944 1102 30-31 -0.13843673389784072 1102 32-33 0.05900290239149086 1102 34-35 0.10563525717168432 1102 36-37 0.19136192930467644 1102 38-39 0.1562700041309384 1102 40-41 0.09242594124041403 1102 42-43 0.18802209244831403 1102 44-45 0.2970225675861755 1102 46-47 0.20677696138105262 1102 48-49 0.15934656744144604 1102 50-51 0.3212421981877398 1102 52-53 0.17394174404402207 1102 54-55 -0.016702064346098666 1102 56-57 -0.10881286957321379 1102 58-59 0.03872243961709643 1102 60-61 0.2837200610434252 1102 62-63 0.10435469019313359 1102 64-65 0.1821055972514145 1102 66-67 0.13029900908482261 1102 68-69 0.09325074274610046 1102 70-71 0.17701818092730548 1102 72-73 0.18672486071735506 1102 74-75 0.24449008513780512 1102 76-77 0.13687166930672134 1102 78-79 -0.013289046973895324 1102 80-81 -0.02967846736021329 1102 82-83 -0.27411390824661197 1102 84-85 -0.067098843082114 1102 86-87 -0.028766852221750128 1102 88-89 -0.05285003581003167 1102 90-91 0.1834669147054271 1102 92-93 0.24316426147164094 1102 94-95 0.30919948894923976 1102 96-97 0.20222074121567601 1102 98-99 0.06235977574049656 1102 100-101 -0.07762656972781912 1103 1 0.1434540569013265 1103 2 0.13122069362999156 1103 3 0.20088632996954203 1103 4 0.12842656570421696 1103 5 0.04658343498690698 1103 6 0.04372755866236133 1103 7 0.15022155108867707 1103 8 0.1088465038962525 1103 9 0.24565925669021738 1103 10-11 0.251733706591061 1103 12-13 0.13029410973005184 1103 14-15 0.22159060056974766 1103 16-17 0.24102551758266344 1103 18-19 0.2111166497954855 1103 20-21 0.21834832909809165 1103 22-23 0.08121129685704176 1103 24-25 0.13144356758443365 1103 26-27 0.17638423124255098 1103 28-29 0.27513817953814623 1103 30-31 0.27800067533591033 1103 32-33 0.17586286185949263 1103 34-35 0.20594047792341286 1103 36-37 0.1468325189878854 1103 38-39 0.1900489980511324 1103 40-41 0.27054891566587713 1103 42-43 0.2907534899819382 1103 44-45 0.32532379257161637 1103 46-47 0.2913325451977826 1103 48-49 0.15824486001030635 1103 50-51 0.22579712561576315 1103 52-53 0.23657743189837532 1103 54-55 0.2567187054337623 1103 56-57 0.14225006989953926 1103 58-59 0.23488154003636197 1103 60-61 0.21543019978758338 1103 62-63 0.09252636045626161 1103 64-65 0.16048128133152773 1103 66-67 0.06379349952206326 1103 68-69 0.13094530046318198 1103 70-71 0.10414139329367345 1103 72-73 0.040467507112552425 1103 74-75 0.009498823312554805 1103 76-77 0.05642282249964836 1103 78-79 0.1827194942842354 1103 80-81 0.2297716840194255 1103 82-83 0.25023544644123064 1103 84-85 0.03514416022444067 1103 86-87 -0.1256252117422605 1103 88-89 0.0845499674719079 1103 90-91 0.20615663155573571 1103 92-93 0.05401026447461987 1103 94-95 0.16290213665720898 1103 96-97 0.0935970242504851 1103 98-99 0.16189230390367015 1103 100-101 0.1736394400031429 1104 1 0.13013228010672862 1104 2 0.15153758445632803 1104 3 0.10575169474409307 1104 4 0.08401444652005097 1104 5 0.19972616288773537 1104 6 0.09015897385812366 1104 7 0.10256554556136166 1104 8 0.09091915207865497 1104 9 0.22358254977704917 1104 10-11 0.09430723623064097 1104 12-13 0.10565513538060856 1104 14-15 0.08765519635424113 1104 16-17 0.1616153657985251 1104 18-19 0.2184858796590632 1104 20-21 0.12037916615709321 1104 22-23 0.11922588040338411 1104 24-25 0.0696315766685558 1104 26-27 0.1400144405925019 1104 28-29 0.07750277749142498 1104 30-31 0.05869637586973653 1104 32-33 0.07230907128380437 1104 34-35 0.04607141071888776 1104 36-37 0.1249951532590714 1104 38-39 0.003044527250757767 1104 40-41 -0.10817404032942335 1104 42-43 0.032042710178920686 1104 44-45 -0.06941372218388153 1104 46-47 0.09631905224170367 1104 48-49 -0.009614252459911654 1104 50-51 0.24137753960630448 1104 52-53 0.23540589940218837 1104 54-55 0.110656293928038 1104 56-57 0.09859625174926379 1104 58-59 -0.055767345671632995 1104 60-61 0.1128999994226163 1104 62-63 0.10286024528124926 1104 64-65 0.01860230586671463 1104 66-67 0.019515767701918207 1104 68-69 -0.03027654790140133 1104 70-71 0.0023299910486684894 1104 72-73 0.0608417158924226 1104 74-75 -0.11787173880617274 1104 76-77 -0.032579953548264484 1104 78-79 0.07858245480300496 1104 80-81 0.2081910872310928 1104 82-83 0.14535714639877995 1104 84-85 0.14554744624722815 1104 86-87 0.151606238154109 1104 88-89 0.23346317259892402 1104 90-91 0.11935943486840728 1104 92-93 0.10180259378330447 1104 94-95 -0.07686931962158638 1104 96-97 -0.07726469696117988 1104 98-99 -0.02507847323483503 1104 100-101 0.03261558643966822 1105 1 0.05984916829503106 1105 2 0.02327670840096374 1105 3 0.028247485235979752 1105 4 0.11966241222300766 1105 5 0.02423744198110711 1105 6 -0.11452089454250114 1105 7 0.06106531866588938 1105 8 0.030745577041933814 1105 9 0.03481671187595481 1105 10-11 0.14917675640642614 1105 12-13 0.0924687019385999 1105 14-15 -0.025147679247325527 1105 16-17 -0.03360169256137624 1105 18-19 -0.10991135759000059 1105 20-21 -0.06598150509525169 1105 22-23 -0.12149528894423156 1105 24-25 -0.04815814795199458 1105 26-27 0.014222172354287466 1105 28-29 0.023999481536144174 1105 30-31 -0.09713230013547047 1105 32-33 0.09821359307173338 1105 34-35 0.12351479134201071 1105 36-37 0.1186891011208715 1105 38-39 0.14828935045141378 1105 40-41 0.022605450111107928 1105 42-43 -0.12312655085834479 1105 44-45 0.009572771564549498 1105 46-47 0.10385623623005102 1105 48-49 0.11394339391751629 1105 50-51 0.11635067040556635 1105 52-53 -0.023008265443344555 1105 54-55 0.032078809124058694 1105 56-57 0.009635313596199069 1105 58-59 -0.09442839368988132 1105 60-61 0.10624128902482965 1105 62-63 0.07169047970010212 1105 64-65 -0.05492675466661723 1105 66-67 0.07479989457230829 1105 68-69 0.07392557118289744 1105 70-71 0.001725060848883686 1105 72-73 0.07442745551195173 1105 74-75 0.09601083084476869 1105 76-77 0.13768382736623863 1105 78-79 0.13466108901167928 1105 80-81 0.08437370524659116 1105 82-83 0.1917606292442926 1105 84-85 0.11961568207521367 1105 86-87 0.2546395470066116 1105 88-89 0.27272019441671347 1105 90-91 0.20029730431991766 1105 92-93 -0.008336125929133686 1105 94-95 -0.1470445814470196 1105 96-97 0.07984399791217811 1105 98-99 0.0919212222475636 1105 100-101 0.08777320415662615 1106 1 0.11903833475741266 1106 2 0.1055547699599515 1106 3 0.08223773089042652 1106 4 0.11256257698080674 1106 5 0.13823529841948812 1106 6 -0.09750601788280733 1106 7 0.080619973786213 1106 8 0.07736590439115787 1106 9 0.07190823796640444 1106 10-11 0.10780541143552824 1106 12-13 0.08873831324703474 1106 14-15 0.12705802285461942 1106 16-17 0.04901290389604185 1106 18-19 0.03428431509497187 1106 20-21 0.1731223536007107 1106 22-23 0.1557909392424648 1106 24-25 0.13647765323267436 1106 26-27 0.16507621205578715 1106 28-29 0.15568421550076295 1106 30-31 0.08742814146102518 1106 32-33 0.11586002940054385 1106 34-35 -0.06787843106960878 1106 36-37 0.06356044466652833 1106 38-39 0.147214897316168 1106 40-41 0.13835332365237463 1106 42-43 0.06932028054711026 1106 44-45 0.19664270526236294 1106 46-47 0.06885250922629638 1106 48-49 0.20203020103688374 1106 50-51 0.22543215995285948 1106 52-53 0.07581523420605407 1106 54-55 0.09378316864951586 1106 56-57 0.13067100731772996 1106 58-59 0.13037656215616522 1106 60-61 0.09827437313111886 1106 62-63 0.045439688691658375 1106 64-65 0.11615625703745991 1106 66-67 0.14216922852895664 1106 68-69 0.18598171567947475 1106 70-71 0.07852538893922656 1106 72-73 0.2103499637640951 1106 74-75 0.11989964213723425 1106 76-77 0.06479614058848426 1106 78-79 0.0029580050764224097 1106 80-81 0.03442205652845587 1106 82-83 0.1162351787257947 1106 84-85 0.04098231662646157 1106 86-87 0.025972724038574313 1106 88-89 0.09205653833293326 1106 90-91 0.10488476134892011 1106 92-93 0.07389294298979365 1106 94-95 0.07138616469485015 1106 96-97 -0.06584194180585712 1106 98-99 0.13396688410071533 1106 100-101 0.13663739442052503 1107 1 0.09930268487409677 1107 2 0.17146476604417416 1107 3 0.14632315579242317 1107 4 0.13086858305913296 1107 5 0.10119151239576496 1107 6 0.054462304854929755 1107 7 0.2279134818701607 1107 8 0.09646578484104396 1107 9 0.16049758350240495 1107 10-11 0.13633832824721281 1107 12-13 0.061098588845375446 1107 14-15 0.061811431071184586 1107 16-17 0.20837547972026016 1107 18-19 0.11940305625810055 1107 20-21 0.009373538131612236 1107 22-23 0.18001834371877834 1107 24-25 0.21397067835962957 1107 26-27 0.14412898259359253 1107 28-29 0.09136146570751436 1107 30-31 0.1328337358970728 1107 32-33 0.029453000966107368 1107 34-35 0.13353419635644315 1107 36-37 0.23765056247145822 1107 38-39 0.2962214322086254 1107 40-41 0.28249712679296834 1107 42-43 0.12927069187117723 1107 44-45 0.1360148209777563 1107 46-47 0.30201843667422423 1107 48-49 0.12813975770996677 1107 50-51 0.2630125648555861 1107 52-53 0.043797979329831094 1107 54-55 0.23286359415278213 1107 56-57 0.15907261663841865 1107 58-59 0.06841969197740383 1107 60-61 0.1208956056194026 1107 62-63 0.05570193855842831 1107 64-65 0.03499399146068782 1107 66-67 0.292262399902981 1107 68-69 0.17720119715556848 1107 70-71 0.2173611202532868 1107 72-73 0.306315474109752 1107 74-75 0.20819957524410881 1107 76-77 0.07126872897735126 1107 78-79 0.25955880250145213 1107 80-81 0.27016321864664405 1107 82-83 0.1646633577153196 1107 84-85 0.31991309178616945 1107 86-87 0.12736103278134436 1107 88-89 -0.03921645094574444 1107 90-91 0.09724078955188986 1107 92-93 0.2644045364028429 1107 94-95 0.27375294872317824 1107 96-97 0.07044886945001139 1107 98-99 0.15691002092278694 1107 100-101 0.22782039787169595 1108 1 0.07414085727606334 1108 2 0.06840729384156674 1108 3 0.09820435001887518 1108 4 0.06349184915863759 1108 5 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-1.0403545831763452 2316 64-65 -0.9463347775990272 2316 66-67 -0.9907223553295736 2316 68-69 -0.9580144061235671 2316 70-71 -1.0383314810317827 2316 72-73 -1.2710394895628667 2316 74-75 -0.9250692022570774 2316 76-77 -0.9803838735909807 2316 78-79 -1.0883684765692223 2316 80-81 -1.1864242583072482 2316 82-83 -1.4589045003477352 2316 84-85 -1.4822706113709572 2316 86-87 -1.1019630132677563 2316 88-89 -0.8016837803644634 2316 90-91 -0.5950988301008877 2316 92-93 -0.6218672527569922 2316 94-95 -0.8024393081861447 2316 96-97 -0.662345501635663 2316 98-99 -0.6743620288999139 2316 100-101 -0.8930945889397641 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 3.0 13 21.0 14 65.0 15 213.0 16 463.0 17 884.0 18 1469.0 19 2191.0 20 3121.0 21 4205.0 22 5101.0 23 5727.0 24 6319.0 25 7575.0 26 9243.0 27 12077.0 28 15097.0 29 18833.0 30 23852.0 31 30053.0 32 38086.0 33 50736.0 34 70184.0 35 106490.0 36 187636.0 37 377837.0 38 596741.0 39 242873.0 40 208.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.26772737282537 20.744990090288482 13.556595463554283 23.430687073331864 2 14.979983877047353 20.624172873269742 41.66292023540611 22.732923014276793 3 18.131023378715827 25.355996333009408 31.096289318074017 25.41669097020075 4 11.856126811099713 16.747746994157815 40.02194672031909 31.374179474423375 5 12.089666891175058 38.75730202541787 36.88733420495415 12.265696878452918 6 30.57259544908282 39.18832512336956 17.80984359248645 12.429235835061167 7 26.754191923860844 33.08989580169306 22.99290597500473 17.16300629944137 8 24.287075800196334 38.43303046710952 20.47345958628826 16.806434146405884 9 24.973752327623778 16.334416256168478 21.157494838508033 37.53433657769971 10-11 23.72993181107839 27.127437126644743 30.11529716547149 19.02733389680538 12-13 23.983466717731318 24.37899768007994 30.087563775790954 21.549971826397783 14-15 21.140271523091346 26.554197866730057 26.855633399805427 25.44989721037317 16-17 21.169683223060094 29.897639580389413 27.70755470741274 21.225122489137753 18-19 21.133338175671216 29.101212565426586 29.01977324652342 20.745676012378777 20-21 21.754092876040552 28.247667974097894 29.000073735599546 20.998165414262004 22-23 21.50593460992311 28.101538384499552 28.76652175097337 21.62600525460397 24-25 21.347831733160767 28.239056316389554 28.84908083622773 21.564031114221947 26-27 21.498632039548692 28.30706915298706 28.682350338743547 21.5119484687207 28-29 21.504453710471395 28.241268702061518 28.797349568938944 21.45692801852814 30-31 21.626262537314748 28.239696066431257 28.64735142346404 21.486689972789954 32-33 21.51649408037788 28.294723473541982 28.73295517231987 21.45582727376027 34-35 21.628274630991843 28.292487112778048 28.70579165621162 21.37344660001849 36-37 21.848188305313805 28.095822162940266 28.579258065794168 21.47673146595176 38-39 21.59572641671399 28.347816325722057 28.61785920242293 21.43859805514102 40-41 21.675184779211403 28.254656348084854 28.64834392044479 21.42181495225895 42-43 21.60304495010191 28.146474117704027 28.692585280173265 21.557895652020793 44-45 21.67345144235637 28.340305199350247 28.500570075232325 21.485673283061065 46-47 21.580649137403537 28.159102714790702 28.46383984187566 21.796408305930104 48-49 21.59199343630438 28.1082668491261 28.53181897873542 21.767920735834096 50-51 21.54203022973609 28.283349430543975 28.484688388270225 21.68993195144971 52-53 21.55412633219318 28.31491043875983 28.40240268001391 21.728560549033073 54-55 21.520163804255894 28.215679272059347 28.523279981026704 21.740876942658055 56-57 21.636886288700186 28.194649876960597 28.310205667296174 21.858258167043047 58-59 21.659254588115143 28.13772489357862 28.398578333619472 21.804442184686767 60-61 21.63249011516238 28.2001052658513 28.421724824719703 21.745679794266614 62-63 21.55685014725109 28.198969462456475 28.399321192271625 21.844859198020806 64-65 21.586729573037864 28.25394100271611 28.328969726583995 21.83035969766203 66-67 21.500778075654924 28.19869432962234 28.270751618881597 22.029775975841137 68-69 21.510682857683687 28.073756509642855 28.294963308285244 22.120597324388214 70-71 21.545542187768255 27.970911856244197 28.359179311771726 22.12436664421583 72-73 21.530272315473912 28.07681048410172 28.19203611503634 22.200881085388026 74-75 21.428335600427886 28.12820529751764 28.241670078313806 22.201789023740663 76-77 21.53349136963326 28.007009284082358 28.224694382447847 22.23480496383654 78-79 21.59466153378004 27.930375870590506 28.149519534738605 22.325443060890848 80-81 21.6098110769478 27.953528968586568 28.071547403566914 22.365112550898715 82-83 21.471160380914647 28.0492019337389 28.19571814957586 22.283919535770593 84-85 21.643544503286186 27.97393831741965 27.977955256797983 22.40456192249618 86-87 21.372483635099027 27.94458164401773 28.196850939633656 22.48608378124959 88-89 21.584363430664325 27.779859616222712 28.030753247667974 22.60502370544499 90-91 21.53230829844649 27.856153951127606 28.018289730281783 22.593248020144124 92-93 21.6614556507882 27.72510818223038 28.066905702072958 22.546530464908457 94-95 21.481188617864706 27.68609434635042 28.095326923838833 22.73739011194605 96-97 21.435709160382633 27.53466123444399 28.272457442453213 22.75717216272016 98-99 21.575834312806222 27.602976717159045 28.013557445534705 22.80763152450003 100-101 21.4221175983765 27.531056994316856 28.055185043338927 22.991640363967726 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 19.0 1 12.0 2 18.5 3 46.0 4 42.0 5 36.0 6 59.5 7 120.5 8 206.5 9 262.5 10 266.0 11 239.5 12 259.0 13 302.0 14 321.0 15 351.0 16 411.0 17 457.5 18 523.5 19 633.5 20 707.5 21 836.5 22 1038.0 23 1306.0 24 1706.0 25 2292.5 26 3304.5 27 4899.0 28 7687.5 29 12511.5 30 19527.5 31 28486.5 32 38972.5 33 50131.5 34 62316.0 35 73904.5 36 82884.0 37 87843.5 38 87674.5 39 84669.0 40 79197.5 41 72457.0 42 66442.5 43 61854.0 44 58893.0 45 57288.5 46 56764.5 47 56199.0 48 56077.0 49 55683.5 50 54314.5 51 53238.0 52 52403.5 53 51257.0 54 49353.0 55 46422.5 56 42209.0 57 37797.5 58 33921.0 59 28928.0 60 23031.5 61 18099.0 62 14092.0 63 10320.0 64 7268.5 65 5082.5 66 3417.5 67 2370.5 68 1693.5 69 1169.5 70 811.5 71 566.0 72 433.0 73 329.0 74 239.0 75 151.0 76 87.5 77 51.5 78 31.0 79 19.0 80 19.5 81 14.5 82 9.0 83 8.0 84 4.5 85 3.5 86 2.0 87 1.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04974401641112329 2 0.0010455047697028124 3 5.502656682646382E-4 4 0.009684675761457632 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0022285759564717846 24-25 0.0 26-27 0.0 28-29 0.021570414195973817 30-31 0.018048713919080132 32-33 2.751328341323191E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03546462231965593 50-51 0.012050818134995575 52-53 0.0 54-55 7.153453687440296E-4 56-57 0.0 58-59 0.0 60-61 2.751328341323191E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.6507970047939145E-4 80-81 0.011830711867689721 82-83 0.005447630115819918 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1817304.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.28661570572747 #Duplication Level Percentage of deduplicated Percentage of total 1 68.41540734623946 36.45625519609867 2 16.14096300806116 17.20194585861836 3 6.350996570660103 10.152693408274738 4 3.1691984000711915 6.755034289591996 5 1.8099821619069132 4.822391194787774 6 1.0883315687445148 3.479610363846028 7 0.7116115068530107 2.654355822821502 8 0.5023545088456858 2.141501732871962 9 0.3626561679291121 1.7392247868375326 >10 1.3960182197458286 11.891004320227786 >50 0.038120359609669 1.367418561607375 >100 0.014048138158868078 1.164971018304764 >500 1.0401439150091784E-4 0.03508449916621255 >1k 2.0802878300183567E-4 0.13850894694528462 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.5026566826463816E-5 0.0 5 0.0 0.0 0.0 5.5026566826463816E-5 0.0 6 0.0 0.0 0.0 1.1005313365292763E-4 0.0 7 0.0 0.0 0.0 1.1005313365292763E-4 0.0 8 0.0 0.0 0.0 1.1005313365292763E-4 0.0 9 0.0 0.0 0.0 1.1005313365292763E-4 0.0 10-11 0.0 0.0 0.0 1.3756641706615954E-4 0.0 12-13 0.0 0.0 0.0 2.2010626730585526E-4 8.253985023969572E-5 14-15 0.0 0.0 0.0 3.02646117545551E-4 1.1005313365292763E-4 16-17 0.0 0.0 0.0 5.777789516778701E-4 1.1005313365292763E-4 18-19 0.0 0.0 0.0 9.07938352636653E-4 1.1005313365292763E-4 20-21 0.0 0.0 0.0 0.0015682571545542188 1.1005313365292763E-4 22-23 0.0 0.0 0.0 0.0037693198276127714 1.1005313365292763E-4 24-25 0.0 0.0 0.0 0.006190488767977179 1.1005313365292763E-4 26-27 0.0 0.0 0.0 0.007896312339597557 1.1005313365292763E-4 28-29 0.0 0.0 0.0 0.010069861729242879 1.1005313365292763E-4 30-31 0.0 0.0 0.0 0.01631537706404652 1.1005313365292763E-4 32-33 0.0 0.0 0.0 0.030897417273059433 1.1005313365292763E-4 34-35 0.0 0.0 0.0 0.0545863542918521 1.1005313365292763E-4 36-37 0.0 0.0 0.0 0.09093140168073147 1.1005313365292763E-4 38-39 0.0 0.0 0.0 0.15162570489032107 1.1005313365292763E-4 40-41 0.0 0.0 0.0 0.2162544076280028 1.1005313365292763E-4 42-43 0.0 0.0 0.0 0.28179104871832117 1.1005313365292763E-4 44-45 0.0 0.0 0.0 0.35588432095015476 1.1005313365292763E-4 46-47 0.0 0.0 0.0 0.448328953218614 1.1005313365292763E-4 48-49 0.0 0.0 0.0 0.5438000466625287 1.1005313365292763E-4 50-51 0.0 0.0 0.0 0.6463970805104704 1.1005313365292763E-4 52-53 0.0 0.0 0.0 0.7478110431716433 1.1005313365292763E-4 54-55 0.0 0.0 0.0 0.8561583532529504 1.1005313365292763E-4 56-57 0.0 0.0 0.0 0.9875893081179593 1.1005313365292763E-4 58-59 0.0 0.0 0.0 1.1329419843900634 1.1005313365292763E-4 60-61 0.0 0.0 0.0 1.2803306436347468 1.1005313365292763E-4 62-63 0.0 0.0 0.0 1.4319013219582413 1.1005313365292763E-4 64-65 0.0 0.0 0.0 1.591670958738879 1.1005313365292763E-4 66-67 0.0 0.0 0.0 1.7692967164547042 1.1005313365292763E-4 68-69 0.0 0.0 0.0 1.9804886799346724 1.1005313365292763E-4 70-71 0.0 0.0 0.0 2.213306084177441 1.1005313365292763E-4 72-73 0.0 0.0 0.0 2.4429594608276877 1.3756641706615954E-4 74-75 0.0 0.0 0.0 2.6764922104392 1.6507970047939145E-4 76-77 0.0 0.0 0.0 2.9252673190616427 1.6507970047939145E-4 78-79 0.0 0.0 0.0 3.2201822039680756 1.6507970047939145E-4 80-81 0.0 0.0 0.0 3.5366950163538955 1.6507970047939145E-4 82-83 0.0 0.0 0.0 3.875438561737607 1.6507970047939145E-4 84-85 0.0 0.0 0.0 4.202021235852669 1.6507970047939145E-4 86-87 0.0 0.0 0.0 4.5474780223892095 1.6507970047939145E-4 88-89 0.0 0.0 0.0 4.955307422423546 1.6507970047939145E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4100 0.0 26.200556 1 TCAACGC 2905 0.0 25.835089 4 GTATCAA 3080 0.0 25.772211 1 CAACGCA 2975 0.0 25.224428 5 AACGCAG 3070 0.0 24.443869 6 GGTATCA 2200 0.0 24.414154 1 ATCAACG 3045 0.0 24.335278 3 ACATGGG 3825 0.0 24.215988 3 TACATGG 4160 0.0 23.86448 2 TATCAAC 3575 0.0 20.993267 2 CATGGGG 2300 0.0 20.858953 4 ACGCAGA 3645 0.0 20.58784 7 CGCAGAG 3770 0.0 19.779236 8 GACCGCG 125 0.006035864 18.998146 7 GCACCGT 275 2.8432623E-8 18.998146 6 GAGTACA 3325 0.0 17.726212 1 GTACTAG 590 0.0 17.72379 1 ATATCGA 245 3.7676327E-6 17.447277 6 GTGCGCG 165 0.0014652135 17.271042 9 TACACTG 1750 0.0 17.09833 5 >>END_MODULE