FastQCFastQC Report
Fri 10 Feb 2017
SRR2031610_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031610_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669590
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13360.1995250825131797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11380.1699547484281426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11500.044.317471
GTATCAA17500.032.389031
CAACGCA20050.028.2083515
TCAACGC19900.028.1821444
ATCAACG20000.028.0412333
AACGCAG21250.027.0586766
TATCAAC22100.024.9708732
ACGCAGA24000.023.95827
CGCAGAG24100.023.4661878
GTGTAAG3950.022.9110771
GTACATG17450.022.3824421
GTATTAA5000.020.957611
ACATGGG17750.020.885333
GCAGAGT27200.020.442299
TACATGG18250.020.3329182
AGAGTAC24900.019.56161110-11
GAGTACT17150.019.53316112-13
GTGGTAT6900.019.3284931
GTACCTG3809.094947E-1218.8016821
TACACTG7600.018.760865
Produced by FastQC (version 0.11.5)