##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031610_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 669590 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.856698875431235 33.0 31.0 34.0 30.0 34.0 2 32.106572678803445 34.0 31.0 34.0 30.0 34.0 3 32.25025762033483 34.0 31.0 34.0 30.0 34.0 4 35.342385638973106 37.0 35.0 37.0 33.0 37.0 5 35.0617452470915 37.0 35.0 37.0 32.0 37.0 6 35.2748816439911 37.0 35.0 37.0 32.0 37.0 7 35.427291327528785 37.0 35.0 37.0 33.0 37.0 8 35.50168013261847 37.0 35.0 37.0 33.0 37.0 9 37.428132140563626 39.0 38.0 39.0 35.0 39.0 10-11 37.376452754670765 39.0 37.5 39.0 34.5 39.0 12-13 37.399454890306004 39.0 38.0 39.0 35.0 39.0 14-15 38.78820173539032 41.0 39.0 41.0 35.0 41.0 16-17 38.75104765602832 41.0 39.0 41.0 35.0 41.0 18-19 38.68881031676101 41.0 38.5 41.0 34.0 41.0 20-21 38.695752624740514 41.0 39.0 41.0 34.0 41.0 22-23 38.648764915844026 40.5 39.0 41.0 34.0 41.0 24-25 38.57675368509088 41.0 38.0 41.0 34.0 41.0 26-27 38.47567466658701 40.0 38.0 41.0 34.0 41.0 28-29 38.37023103690318 40.0 38.0 41.0 34.0 41.0 30-31 38.236117624217805 40.0 38.0 41.0 34.0 41.0 32-33 38.07404232440747 40.0 38.0 41.0 33.0 41.0 34-35 37.940786899445925 40.0 38.0 41.0 33.0 41.0 36-37 37.81109559581236 40.0 38.0 41.0 32.5 41.0 38-39 37.645896742782895 40.0 38.0 41.0 32.0 41.0 40-41 37.49820263146104 40.0 37.0 41.0 31.0 41.0 42-43 37.32863767380039 40.0 37.0 41.0 31.0 41.0 44-45 37.1236256515181 40.0 37.0 41.0 30.5 41.0 46-47 36.91052659089891 40.0 37.0 41.0 30.0 41.0 48-49 37.01530414134022 40.0 37.0 41.0 30.0 41.0 50-51 36.79116698278051 39.5 36.5 40.5 30.0 41.0 52-53 36.919766573574876 39.5 37.0 40.5 30.5 41.0 54-55 37.2074231992712 40.0 37.0 41.0 31.0 41.0 56-57 37.12957780134112 40.0 37.0 41.0 30.5 41.0 58-59 36.95543392225093 40.0 36.0 41.0 30.0 41.0 60-61 36.69895085052047 40.0 36.0 41.0 29.0 41.0 62-63 36.39750593646859 39.0 35.0 41.0 28.5 41.0 64-65 36.149737152585914 39.0 35.0 41.0 28.0 41.0 66-67 35.947485774877165 39.0 35.0 41.0 28.0 41.0 68-69 35.71641900267328 38.0 35.0 40.5 28.0 41.0 70-71 35.411336041458206 37.0 35.0 40.0 28.0 41.0 72-73 35.047294613121466 37.0 34.5 39.5 28.0 41.0 74-75 34.621937304917935 36.0 34.0 39.0 27.0 41.0 76-77 34.209490882480324 36.0 34.0 39.0 26.0 40.5 78-79 33.83592272883406 35.0 34.0 37.5 26.0 39.5 80-81 33.497134067115695 35.0 34.0 37.0 26.0 39.0 82-83 33.15154273510656 35.0 34.0 36.5 26.0 39.0 84-85 32.863619528368105 35.0 33.0 36.0 25.5 37.0 86-87 32.61290565868666 35.0 33.0 36.0 25.0 37.0 88-89 32.34885601636823 35.0 33.0 35.5 25.0 37.0 90-91 32.13177168117804 35.0 33.0 35.0 24.5 36.0 92-93 31.965144342060064 35.0 33.0 35.0 24.0 36.0 94-95 31.81505996206634 35.0 32.0 35.0 24.0 36.0 96-97 31.70995161218059 35.0 32.0 35.0 23.5 36.0 98-99 31.603989008199047 35.0 32.0 35.0 23.0 35.0 100-101 31.150702668797322 34.5 31.5 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.43846961949037677 1101 2 0.5495605912698949 1101 3 0.5225497791626381 1101 4 0.452130948474597 1101 5 0.4455377140452157 1101 6 0.43692287530748786 1101 7 0.5326601760583003 1101 8 0.4169240155385907 1101 9 0.5160192680853868 1101 10-11 0.5875000133882224 1101 12-13 0.47390520922986923 1101 14-15 0.6643915015368549 1101 16-17 0.566097245795234 1101 18-19 0.5576437458909069 1101 20-21 0.45464807493342363 1101 22-23 0.6641257355343626 1101 24-25 0.8131359665517763 1101 26-27 0.7710708663631607 1101 28-29 0.6155801953329885 1101 30-31 0.5408453489313985 1101 32-33 0.7330562325126735 1101 34-35 0.5089063201451012 1101 36-37 0.367948044802084 1101 38-39 0.38784071675866727 1101 40-41 0.5975958142203268 1101 42-43 0.511453841748363 1101 44-45 0.4725424123286004 1101 46-47 0.534353774365222 1101 48-49 0.4231899782203712 1101 50-51 0.4217799512328426 1101 52-53 0.4701655132614917 1101 54-55 0.39812249762102425 1101 56-57 0.5655295101000704 1101 58-59 0.5215280152512065 1101 60-61 0.47602959657452004 1101 62-63 0.3802252237784458 1101 64-65 0.3989442283186406 1101 66-67 0.29539961578793594 1101 68-69 0.46444978980827045 1101 70-71 0.5679757035215047 1101 72-73 0.5828510513928506 1101 74-75 0.49804260820449286 1101 76-77 0.5108047211884781 1101 78-79 0.5650017637424085 1101 80-81 0.43543810704503727 1101 82-83 0.5264804043000666 1101 84-85 0.541049157882135 1101 86-87 0.5434989630731408 1101 88-89 0.399996607527747 1101 90-91 0.3939557184231788 1101 92-93 0.2557536369289686 1101 94-95 0.34652525143681956 1101 96-97 0.20023834162036636 1101 98-99 0.26810391952426116 1101 100-101 0.5464010794517797 1102 1 0.039355852290263726 1102 2 -0.024306047380115103 1102 3 0.007323446800619138 1102 4 0.15721442678383113 1102 5 0.2118077184022198 1102 6 0.08652719152078703 1102 7 0.07333016558295924 1102 8 0.13702524631447943 1102 9 0.16047861354622484 1102 10-11 0.058729893153497414 1102 12-13 0.18651301413505195 1102 14-15 0.05894741176406626 1102 16-17 0.21930149649899278 1102 18-19 0.36408314802367414 1102 20-21 0.05271307151888749 1102 22-23 -0.06350136687373009 1102 24-25 0.17762238104793227 1102 26-27 0.16704820995141745 1102 28-29 0.09582381665078543 1102 30-31 0.37175967049053327 1102 32-33 0.1770193123261592 1102 34-35 -0.1191431951770312 1102 36-37 0.02232961313749371 1102 38-39 -0.01029040737881104 1102 40-41 0.22253053643825638 1102 42-43 0.08888951416359703 1102 44-45 -0.5757971372006239 1102 46-47 -0.9236490499610639 1102 48-49 -0.4263420302958707 1102 50-51 -0.22809938143509356 1102 52-53 -0.13436700511671518 1102 54-55 -0.04670889257169364 1102 56-57 -0.1570916748191351 1102 58-59 -0.2617976444020229 1102 60-61 -0.39107410934945364 1102 62-63 -0.7204447039447572 1102 64-65 -0.34780578790423533 1102 66-67 -0.27193769961886005 1102 68-69 5.155712721602868E-4 1102 70-71 -0.04390809739130219 1102 72-73 -0.36452501352339084 1102 74-75 -0.3018844660839406 1102 76-77 -0.21922800267955722 1102 78-79 -0.12622737440546672 1102 80-81 0.06308591293903731 1102 82-83 -0.2773478865499257 1102 84-85 -0.23312988710005556 1102 86-87 0.03203942911788005 1102 88-89 -0.21186092776860477 1102 90-91 -0.28836165056547003 1102 92-93 -0.0790773515543357 1102 94-95 0.04377230582518621 1102 96-97 -0.1169406075695747 1102 98-99 -0.21139962942402946 1102 100-101 -0.1606183292937402 1103 1 0.11162451879300761 1103 2 0.21417850306657016 1103 3 0.13821435738171317 1103 4 0.44194518655761783 1103 5 0.24395672258220458 1103 6 0.3217743024133384 1103 7 0.4258012745084514 1103 8 0.31621137859871595 1103 9 0.4591426574770523 1103 10-11 0.5155981826379659 1103 12-13 0.4375375203230689 1103 14-15 0.5127012024369648 1103 16-17 0.5823147740991956 1103 18-19 0.4097044085760828 1103 20-21 0.32396132985837056 1103 22-23 0.3364760939901288 1103 24-25 0.4454180535337926 1103 26-27 0.23712443321180388 1103 28-29 0.39028738521074047 1103 30-31 0.47732478810384293 1103 32-33 0.4364447692559139 1103 34-35 0.4515579768580551 1103 36-37 0.4977004843775532 1103 38-39 0.44447764442022475 1103 40-41 0.2963769424705518 1103 42-43 0.6645288060249186 1103 44-45 0.6097204433255641 1103 46-47 0.6122598919289359 1103 48-49 0.3497828774985763 1103 50-51 0.47925042595774414 1103 52-53 0.4196244709917991 1103 54-55 0.376944906297247 1103 56-57 0.43984710069107535 1103 58-59 0.5121758003975785 1103 60-61 0.43855051158325153 1103 62-63 0.4410482064724235 1103 64-65 0.4387178576985775 1103 66-67 0.001584962458018424 1103 68-69 0.2758925895493505 1103 70-71 0.38624107070967995 1103 72-73 0.38995207851824176 1103 74-75 0.12208692970329338 1103 76-77 -0.24880549632761984 1103 78-79 0.0700167859092673 1103 80-81 0.3835719386746348 1103 82-83 0.19001134629984762 1103 84-85 0.13067226026490886 1103 86-87 0.2460997520422339 1103 88-89 0.2439828555278467 1103 90-91 0.2652200230678403 1103 92-93 0.045461620661054525 1103 94-95 -0.10846931371419188 1103 96-97 -0.038934706413929376 1103 98-99 0.16990108363227563 1103 100-101 0.2941599675671718 1104 1 0.024209552806613743 1104 2 0.16806005568761861 1104 3 0.3147649776298138 1104 4 0.19896799568127221 1104 5 -0.11624735905045469 1104 6 0.027116439268077386 1104 7 0.015357096837384177 1104 8 0.2239544758376084 1104 9 0.2499389760044579 1104 10-11 0.1339855375799388 1104 12-13 0.3078656515755753 1104 14-15 0.4977952400520138 1104 16-17 0.36787899978946825 1104 18-19 0.38366399240961613 1104 20-21 0.3056339989259911 1104 22-23 0.2614961019933304 1104 24-25 0.22287903792754804 1104 26-27 0.3887450814711073 1104 28-29 0.5282425672835629 1104 30-31 0.45939761723549566 1104 32-33 0.33346357677892513 1104 34-35 0.3037888692149977 1104 36-37 0.2854155704119776 1104 38-39 0.3314024143281884 1104 40-41 0.2037799036550254 1104 42-43 0.22578330782563683 1104 44-45 0.22700735809146977 1104 46-47 0.4775760183795157 1104 48-49 0.2580661908239037 1104 50-51 0.2598726748573057 1104 52-53 0.15385816446919165 1104 54-55 0.18222701914238115 1104 56-57 0.14879067812204028 1104 58-59 0.10911457590778184 1104 60-61 0.40075539353603773 1104 62-63 0.6256320400058328 1104 64-65 0.43194514861494326 1104 66-67 0.26278944425073547 1104 68-69 0.1700002326065757 1104 70-71 0.37685731720827675 1104 72-73 0.4098700457051194 1104 74-75 0.3251581581946894 1104 76-77 0.33071631239587873 1104 78-79 0.30870226009894 1104 80-81 0.21416417556938683 1104 82-83 0.5371301938388555 1104 84-85 0.5346538529019682 1104 86-87 0.34167798333475474 1104 88-89 0.17481518846101807 1104 90-91 0.16039809429633323 1104 92-93 0.29396102042096217 1104 94-95 0.15127458384483106 1104 96-97 0.36832819874813083 1104 98-99 0.47080144377633815 1104 100-101 0.3533636490397605 1105 1 0.11479396839102662 1105 2 0.14962995637516485 1105 3 0.11741967587274615 1105 4 0.05187097505483962 1105 5 -0.25326034606343484 1105 6 -0.2501180909076268 1105 7 0.10298123740270171 1105 8 0.0928811374113323 1105 9 0.1427388232160709 1105 10-11 0.14102335270599298 1105 12-13 0.14026633912332187 1105 14-15 0.15235024387172302 1105 16-17 0.16533889283760317 1105 18-19 0.28393137208876595 1105 20-21 -0.09343972147124902 1105 22-23 0.032170280755750014 1105 24-25 0.06369263609408904 1105 26-27 -0.06911397124088126 1105 28-29 0.06858825101160448 1105 30-31 0.010333446112497313 1105 32-33 0.2187576944259817 1105 34-35 0.3270604505748054 1105 36-37 0.15072305704298827 1105 38-39 0.26814802166202156 1105 40-41 0.4783883834105609 1105 42-43 0.3654796409043257 1105 44-45 0.3607640425834475 1105 46-47 0.4401046662022807 1105 48-49 0.5173385361256635 1105 50-51 0.5285701538489036 1105 52-53 0.48614426072587236 1105 54-55 0.656281640991125 1105 56-57 0.7593044290769697 1105 58-59 0.7446665239597365 1105 60-61 0.6318096334138019 1105 62-63 0.5787260048645066 1105 64-65 0.4847908323429806 1105 66-67 0.20640097977403826 1105 68-69 0.31643835330940107 1105 70-71 0.33699864646725075 1105 72-73 0.48151825044156027 1105 74-75 0.44401797484862726 1105 76-77 0.5146066867274257 1105 78-79 0.5384768972265164 1105 80-81 0.6097237630407406 1105 82-83 0.5749835169863005 1105 84-85 0.3725167253236634 1105 86-87 0.5171649963217604 1105 88-89 0.4442766093930288 1105 90-91 0.3512976359311679 1105 92-93 0.5696963145386142 1105 94-95 0.6548269138677476 1105 96-97 0.6035745700239232 1105 98-99 0.5045485789940543 1105 100-101 0.8995248407891872 1106 1 0.3047708594548766 1106 2 0.1409013661248366 1106 3 0.07393357260404088 1106 4 0.19031023305931427 1106 5 0.3921952450443271 1106 6 -0.04114429497730754 1106 7 0.09682233993945744 1106 8 0.2859688468033852 1106 9 0.48566341070587526 1106 10-11 0.4891513720296601 1106 12-13 0.43590256682476536 1106 14-15 0.3819952968863376 1106 16-17 0.22046834470618393 1106 18-19 0.40215815335902505 1106 20-21 0.34182258063887616 1106 22-23 0.5425418536273199 1106 24-25 0.5324772733569816 1106 26-27 0.19699823780610615 1106 28-29 0.44417512388108804 1106 30-31 0.5934968607976501 1106 32-33 0.3602920859603742 1106 34-35 0.5509491826157102 1106 36-37 0.543774952812278 1106 38-39 0.7557884006067539 1106 40-41 0.6967351533877704 1106 42-43 0.6182284018052613 1106 44-45 0.5669158818874607 1106 46-47 0.6434267064808452 1106 48-49 0.30610743630760595 1106 50-51 0.18284321573066364 1106 52-53 0.280489106701161 1106 54-55 0.566895282583026 1106 56-57 0.462876119061697 1106 58-59 0.7446103200991843 1106 60-61 0.5961058042317191 1106 62-63 0.5461329940106268 1106 64-65 0.8292211604689612 1106 66-67 0.6231055513699104 1106 68-69 0.41433371479171655 1106 70-71 0.28266389104553724 1106 72-73 0.3620223075760478 1106 74-75 0.5514183240967938 1106 76-77 0.4814477160684518 1106 78-79 0.30261151462200786 1106 80-81 0.39373494107366014 1106 82-83 0.4579383238541439 1106 84-85 0.4171177563377384 1106 86-87 0.12548290627010772 1106 88-89 0.29456782381468116 1106 90-91 0.49947846074243074 1106 92-93 0.48077004354712116 1106 94-95 0.523822250254387 1106 96-97 0.634887962750355 1106 98-99 0.4295464876876167 1106 100-101 0.46254374619939753 1107 1 0.3286267042825912 1107 2 0.2637290925331257 1107 3 0.2805482140012856 1107 4 0.31571007712923205 1107 5 0.3316302535330422 1107 6 0.2519386758550226 1107 7 0.24392852540881194 1107 8 0.28432939503606036 1107 9 0.3499605230848317 1107 10-11 0.3922488429020703 1107 12-13 0.3803069357815545 1107 14-15 0.49251620536121266 1107 16-17 0.26294471838460254 1107 18-19 0.44944722878108934 1107 20-21 0.4101804328841965 1107 22-23 0.5005609587052504 1107 24-25 0.38825287977197576 1107 26-27 0.44657744518582376 1107 28-29 0.43095749941026185 1107 30-31 0.4707529285908052 1107 32-33 0.42598673989032676 1107 34-35 0.505383353162415 1107 36-37 0.48149400178451884 1107 38-39 0.6505793158580317 1107 40-41 0.6320496731012639 1107 42-43 0.6757294401247123 1107 44-45 0.4538119260725111 1107 46-47 0.6635919632189982 1107 48-49 0.4537783218301499 1107 50-51 0.29939002025700034 1107 52-53 0.3401542213534796 1107 54-55 0.36610980302516793 1107 56-57 0.10535176409489821 1107 58-59 0.3468866077974653 1107 60-61 0.4316323597071161 1107 62-63 0.5281099542484569 1107 64-65 0.35474195964705757 1107 66-67 0.29112385493808546 1107 68-69 0.35196532633637645 1107 70-71 0.3118497757301455 1107 72-73 0.4431387377973408 1107 74-75 0.5302001750014043 1107 76-77 0.13713734234632113 1107 78-79 0.3166962269164415 1107 80-81 0.2968834171121628 1107 82-83 0.5898558995057428 1107 84-85 0.6051774469549756 1107 86-87 0.3069009956165871 1107 88-89 0.5011241735935315 1107 90-91 0.3546019296766403 1107 92-93 0.26864320098549754 1107 94-95 0.34468117725142733 1107 96-97 0.2994852769640346 1107 98-99 0.13664932138891572 1107 100-101 0.18846384296348262 1108 1 0.18855885335769784 1108 2 0.18453014520710553 1108 3 0.008730516367563723 1108 4 -0.07207407099703289 1108 5 0.08294721492156754 1108 6 0.16436801645681953 1108 7 -0.022784688066487035 1108 8 0.09158532639742134 1108 9 0.1927262986486511 1108 10-11 0.1400960530027291 1108 12-13 0.10353804516208953 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Count 6 5.0 7 46.0 8 156.0 9 198.0 10 192.0 11 256.0 12 398.0 13 564.0 14 777.0 15 1009.0 16 1322.0 17 1745.0 18 2162.0 19 2913.0 20 3646.0 21 4615.0 22 5101.0 23 5399.0 24 5303.0 25 5374.0 26 5739.0 27 6509.0 28 7520.0 29 8747.0 30 10591.0 31 12534.0 32 15774.0 33 20344.0 34 27906.0 35 41020.0 36 68988.0 37 131339.0 38 196262.0 39 74973.0 40 163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.36067758355743 20.051833221283136 14.965754658086821 22.62173453707261 2 14.31319051973556 20.768435096766456 42.067249314164926 22.851125069333055 3 18.069809971057175 26.150305141765962 30.955502279209952 24.824382607966907 4 11.735982312198088 18.186431468000734 40.842715382968755 29.23487083683242 5 12.653178567578252 38.35603310933027 36.204046429726525 12.786741893364958 6 28.935191420712524 38.97493916619526 19.133391398563933 12.95647801452828 7 26.06545430603502 33.41488560983895 22.92756212562642 17.592097958499618 8 24.406401747835783 36.85673881976192 20.89352118577489 17.84333824662741 9 25.192846678847246 17.299113990156776 21.193157927027265 36.31488140396871 10-11 23.667797335632084 27.449739108051098 29.49287513927582 19.389588417041 12-13 24.548603836829976 24.690913716218237 29.473857480621312 21.28662496633047 14-15 21.65742028078882 26.503878393688012 27.214006815436946 24.62469451008622 16-17 21.24737846778449 29.669765418645426 27.77217222065267 21.310683892917414 18-19 21.000332423215838 28.839394765641636 29.60000896422155 20.560263846920975 20-21 21.998842570985868 27.85677749360614 29.68066160135904 20.46371833404895 22-23 21.58066876745471 27.85980973431503 29.229379172329335 21.330142325900926 24-25 21.36620917277737 27.848832867874375 29.566824474678533 21.218133484669725 26-27 21.380919667259068 28.074866709478936 29.224749473558447 21.31946414970355 28-29 21.360310040472527 28.03551426992637 29.363565764124317 21.240609925476782 30-31 21.6933496617333 27.934631640257475 29.36953956898998 21.00247912901925 32-33 21.432742424468703 28.025134783972284 29.21750623515883 21.324616556400187 34-35 21.571558715034573 28.158051942233307 29.20436386445437 21.06602547827775 36-37 21.71978374826386 27.987574485879417 29.058154990367242 21.23448677548948 38-39 21.54631938947714 28.25609701459102 29.064726175719468 21.13285742021237 40-41 21.77601218656193 27.994444361474933 29.066891679983275 21.162651771979867 42-43 21.647351364267685 28.034916889439803 28.980420854552786 21.337310891739723 44-45 21.749404387120702 28.234368620436438 28.77606600871322 21.240160983729638 46-47 21.66301232498447 28.21089417874595 28.49600766289259 21.630085833376985 48-49 21.579685698121022 28.23739380708558 28.757044961446564 21.425875533346836 50-51 21.756546532948978 28.352767827655413 28.44265516586941 21.4480304735262 52-53 21.777597772585192 28.3680046748212 28.39393438960181 21.460463162991797 54-55 21.639779421710625 28.334251288562527 28.529375937416628 21.49659335231022 56-57 21.757078204497578 28.445060095776476 28.252565227727032 21.545296471998913 58-59 21.78856088781526 28.44082228148432 28.31428409131678 21.456332739383647 60-61 21.819954787912234 28.381989047697033 28.451609381321262 21.346446783069474 62-63 21.63207528808682 28.485974060583196 28.374512120082464 21.50743853124752 64-65 21.737192831335392 28.30662431507669 28.385360586701125 21.5708222668868 66-67 21.61333653261813 28.296029523119692 28.359566721159158 21.73106722310302 68-69 21.805490355548514 28.221868730209515 28.275017118116498 21.697623796125477 70-71 21.653155196678025 28.104879002151723 28.39142630151443 21.85053949965583 72-73 21.581693006794954 28.214114642142164 28.467365466571117 21.73682688449177 74-75 21.589278490506217 28.270556152507687 28.227186770244717 21.91297858674138 76-77 21.557610300362388 28.04015806419993 28.420876985436454 21.981354650001233 78-79 21.626199000421725 28.250648290218667 28.153217822522514 21.96993488683709 80-81 21.684492318316238 28.261287108956072 28.06129503091432 21.992925541813367 82-83 21.64615210991388 28.272195743923923 28.267188243884682 21.814463902277513 84-85 21.726625372881088 28.252707802492427 28.119662783197164 21.901004041429324 86-87 21.541871321169516 28.140134251859354 28.296011675114386 22.021982751856743 88-89 21.754988889495134 28.132411207520914 28.23951752081398 21.873082382169976 90-91 21.687440521089453 28.19201306162482 28.188499460624865 21.93204695666085 92-93 21.682966012199827 28.13559220623035 28.222050037844 21.959391743725824 94-95 21.622159150384462 28.189348103707644 28.188226568131043 22.000266177776847 96-97 21.54294597745822 28.11025740679841 28.439564710454725 21.907231905288647 98-99 21.550357704146947 28.03925217301626 28.200673035317543 22.209717087519255 100-101 21.65642264832158 28.046568067056104 28.163952113884193 22.13305717073812 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8.0 1 6.0 2 14.5 3 38.0 4 39.5 5 29.5 6 50.5 7 98.0 8 165.0 9 223.5 10 249.0 11 273.5 12 293.5 13 330.5 14 373.5 15 418.0 16 477.5 17 526.5 18 587.5 19 662.5 20 752.5 21 918.5 22 1126.0 23 1428.5 24 1895.5 25 2452.0 26 3162.5 27 4169.5 28 5430.5 29 6994.0 30 9015.5 31 11390.5 32 14271.5 33 17000.0 34 19491.0 35 21583.0 36 23343.5 37 24870.5 38 25639.5 39 25921.5 40 25791.0 41 25662.0 42 25275.5 43 24945.5 44 25159.5 45 25539.0 46 25629.5 47 25503.0 48 25328.0 49 24475.0 50 23414.5 51 22213.5 52 20365.5 53 18851.5 54 17545.5 55 15872.0 56 13935.5 57 12006.5 58 10408.5 59 8689.5 60 6940.0 61 5607.5 62 4302.0 63 2978.5 64 2040.0 65 1479.0 66 1063.5 67 735.5 68 554.5 69 426.5 70 312.0 71 217.0 72 162.0 73 121.0 74 85.5 75 60.0 76 40.5 77 34.0 78 22.0 79 17.0 80 14.0 81 11.0 82 9.0 83 4.5 84 5.5 85 3.0 86 4.0 87 4.5 88 1.0 89 2.0 90 2.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4811899819292403 2 0.37634970653683597 3 0.20549888737884375 4 0.23282904463925685 5 0.26015920189966996 6 0.20549888737884375 7 0.19519407398557326 8 0.1993757373915381 9 0.19609014471542288 10-11 0.2093071879807046 12-13 0.1986290117833301 14-15 0.2092325154198838 16-17 0.09124986932301857 18-19 0.03920309443092042 20-21 3.7336280410400394E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.14202721068116309 46-47 0.21431024955569827 48-49 0.08714287847787452 50-51 0.06257560596783106 52-53 0.07071491509729835 54-55 0.07922758703086964 56-57 0.11066473513642676 58-59 0.09289266566107618 60-61 0.1438940247016831 62-63 0.246270105587001 64-65 0.13470929972072462 66-67 0.1031228064935259 68-69 0.10573634612225391 70-71 0.08818829432936573 72-73 0.07325378216520557 74-75 0.13672545886288626 76-77 0.206170940426231 78-79 0.13560537045057422 80-81 0.08415597604504249 82-83 0.08863632969429053 84-85 0.09610358577637061 86-87 0.07086426021893995 88-89 0.0938634089517466 90-91 0.113576965008438 92-93 0.15770844845353127 94-95 0.12903418509834375 96-97 0.09110052420137696 98-99 0.07937693215251124 100-101 0.06257560596783106 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 669590.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.85267350457472 #Duplication Level Percentage of deduplicated Percentage of total 1 80.85208766041669 59.711428321480085 2 12.551784741644017 18.539657208486773 3 3.6027236415266164 7.9821231847463325 4 1.3694453075657138 4.045487887280904 5 0.6484116298846712 2.3943466199220884 6 0.32698521177189194 1.4489239251488257 7 0.19574451003770907 1.0119378773089516 8 0.11880040524988172 0.7018982032904545 9 0.07844574228484319 0.5214085013507883 >10 0.2494839991732055 2.7989873099867393 >50 0.004463934309331224 0.22882245452241545 >100 0.0010145100853658489 0.1461023499706913 >500 2.0290201668933064E-4 0.09815080936744876 >1k 4.058040333786613E-4 0.3707253471375087 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1336 0.1995250825131797 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1138 0.1699547484281426 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.4934512164160158E-4 0.0 14-15 0.0 0.0 0.0 2.9869024328320315E-4 0.0 16-17 0.0 0.0 0.0 8.960707298496095E-4 0.0 18-19 0.0 0.0 0.0 0.0012694335339536135 0.0 20-21 0.0 0.0 0.0 0.002090831702982422 0.0 22-23 0.0 0.0 0.0 0.003509610358577637 0.0 24-25 0.0 0.0 0.0 0.005525769500739258 0.0 26-27 0.0 0.0 0.0 0.0062724951089472665 0.0 28-29 0.0 0.0 0.0 0.00754192864290088 0.0 30-31 0.0 0.0 0.0 0.0094834152242417 0.0 32-33 0.0 0.0 0.0 0.013739751191027345 0.0 34-35 0.0 0.0 0.0 0.02113233471228662 0.0 36-37 0.0 0.0 0.0 0.03322928956525635 0.0 38-39 0.0 0.0 0.0 0.04935856270254932 0.0 40-41 0.0 0.0 0.0 0.06937080900252393 0.0 42-43 0.0 0.0 0.0 0.09087650651891456 0.0 44-45 0.0 0.0 0.0 0.11469705342075001 0.0 46-47 0.0 0.0 0.0 0.1418031929987007 0.0 48-49 0.0 0.0 0.0 0.17226959781358742 0.0 50-51 0.0 0.0 0.0 0.2093818605415254 0.0 52-53 0.0 0.0 0.0 0.24373123851909378 0.0 54-55 0.0 0.0 0.0 0.2838304036798638 0.0 56-57 0.0 0.0 0.0 0.3325169133350259 0.0 58-59 0.0 0.0 0.0 0.38575844920025687 0.0 60-61 0.0 0.0 0.0 0.44975283382368314 0.0 62-63 0.0 0.0 0.0 0.5126271300347974 0.0 64-65 0.0 0.0 0.0 0.5764721695365821 0.0 66-67 0.0 0.0 0.0 0.6487552084111172 0.0 68-69 0.0 0.0 0.0 0.7359727594498126 0.0 70-71 0.0 0.0 0.0 0.8316283098612585 0.0 72-73 0.0 0.0 0.0 0.9339297181857555 0.0 74-75 0.0 0.0 0.0 1.0410101704027839 0.0 76-77 0.0 0.0 0.0 1.1651906390477755 0.0 78-79 0.0 0.0 0.0 1.3049030003434938 0.0 80-81 0.0 0.0 0.0 1.46679311220299 0.0 82-83 0.0 0.0 0.0 1.6368225331919533 0.0 84-85 0.0 0.0 0.0 1.7990860078555535 0.0 86-87 0.0 0.0 0.0 1.976508012365776 7.467256082080079E-5 88-89 0.0 0.0 0.0 2.181036156453949 1.4934512164160158E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1150 0.0 44.31747 1 GTATCAA 1750 0.0 32.38903 1 CAACGCA 2005 0.0 28.208351 5 TCAACGC 1990 0.0 28.182144 4 ATCAACG 2000 0.0 28.041233 3 AACGCAG 2125 0.0 27.058676 6 TATCAAC 2210 0.0 24.970873 2 ACGCAGA 2400 0.0 23.9582 7 CGCAGAG 2410 0.0 23.466187 8 GTGTAAG 395 0.0 22.911077 1 GTACATG 1745 0.0 22.382442 1 GTATTAA 500 0.0 20.95761 1 ACATGGG 1775 0.0 20.88533 3 GCAGAGT 2720 0.0 20.44229 9 TACATGG 1825 0.0 20.332918 2 AGAGTAC 2490 0.0 19.561611 10-11 GAGTACT 1715 0.0 19.533161 12-13 GTGGTAT 690 0.0 19.328493 1 GTACCTG 380 9.094947E-12 18.801682 1 TACACTG 760 0.0 18.76086 5 >>END_MODULE