FastQCFastQC Report
Fri 10 Feb 2017
SRR2031610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669590
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15350.22924476171985844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14400.2150569751639063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6990.10439224002747949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9000.044.347351
GTATCAA16950.041.2077141
TCAACGC19550.035.2281574
CAACGCA19650.035.046265
ATCAACG20050.034.349653
AACGCAG20500.033.8248026
TATCAAC21050.032.945942
CTTATCG600.003952339331.6624839
ACGCAGA23250.029.8240187
CGCAGAG23700.029.2577428
TAACGTC700.00839451927.139275
GCAGAGT26550.025.4015439
GTATAGG2451.0913936E-1125.2120481
GGCCGAC950.001226964224.9966987
GTGGTAT5400.024.6374151
TAGCCCT6000.023.7486384
TGGTATC6000.022.958732
TACATGG18000.021.1114752
AGAGTAC24300.021.10832410-11
GTAGCCC4750.020.9987963
Produced by FastQC (version 0.11.5)