##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031610_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 669590 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.441721053181794 34.0 31.0 34.0 31.0 34.0 2 32.666903627593 34.0 31.0 34.0 31.0 34.0 3 32.77305216625099 34.0 31.0 34.0 31.0 34.0 4 36.22919547782972 37.0 37.0 37.0 35.0 37.0 5 35.895301602473154 37.0 35.0 37.0 35.0 37.0 6 35.95196762197763 37.0 36.0 37.0 35.0 37.0 7 35.928015651368746 37.0 36.0 37.0 35.0 37.0 8 36.05083259905315 37.0 36.0 37.0 35.0 37.0 9 37.94760077062083 39.0 39.0 39.0 35.0 39.0 10-11 37.9280746426918 39.0 38.0 39.0 35.0 39.0 12-13 37.94433832643857 39.0 38.0 39.0 35.0 39.0 14-15 39.38816365238429 41.0 39.0 41.0 36.0 41.0 16-17 39.35339685479174 41.0 39.0 41.0 36.0 41.0 18-19 39.380215505010526 41.0 39.0 41.0 36.0 41.0 20-21 39.4031840379934 41.0 39.0 41.0 36.0 41.0 22-23 39.34338475783689 41.0 39.0 41.0 36.0 41.0 24-25 39.278613031855315 41.0 39.0 41.0 36.0 41.0 26-27 39.16305351035709 41.0 39.0 41.0 36.0 41.0 28-29 39.0743365342971 40.5 39.0 41.0 36.0 41.0 30-31 38.95743813378336 40.0 38.5 41.0 35.0 41.0 32-33 38.801220896369415 40.0 38.0 41.0 35.0 41.0 34-35 38.68093012141759 40.0 38.0 41.0 35.0 41.0 36-37 38.54632611000761 40.0 38.0 41.0 34.5 41.0 38-39 38.43076584178378 40.0 38.0 41.0 34.0 41.0 40-41 38.320422198658875 40.0 38.0 41.0 34.0 41.0 42-43 38.16912065592378 40.0 38.0 41.0 33.5 41.0 44-45 38.109930703863554 40.0 38.0 41.0 33.0 41.0 46-47 38.08813527681119 40.0 38.0 41.0 33.0 41.0 48-49 38.16648919488045 40.0 38.0 41.0 33.0 41.0 50-51 38.08951149210711 40.0 38.0 41.0 33.0 41.0 52-53 37.99962066339103 40.0 38.0 41.0 33.0 41.0 54-55 37.90315118206664 40.0 38.0 41.0 33.0 41.0 56-57 37.813007213369374 40.0 37.0 41.0 33.0 41.0 58-59 37.64195477829716 40.0 37.0 41.0 33.0 41.0 60-61 37.37411102316343 40.0 36.5 41.0 32.0 41.0 62-63 37.202689705640765 39.5 36.0 41.0 32.0 41.0 64-65 36.926955301005094 39.0 36.0 41.0 31.0 41.0 66-67 36.664428978927404 39.0 35.0 41.0 31.0 41.0 68-69 36.38399468331367 38.5 35.0 40.5 31.0 41.0 70-71 36.049831240012544 37.5 35.0 40.0 31.0 41.0 72-73 35.67071566182291 37.0 35.0 39.5 30.0 41.0 74-75 35.29948027897669 36.5 35.0 39.0 30.0 41.0 76-77 34.66242775429741 36.0 34.0 39.0 29.0 40.0 78-79 34.59963485117758 35.5 34.0 37.5 30.0 39.0 80-81 34.305802804701386 35.0 34.0 37.0 29.0 39.0 82-83 34.00296002031094 35.0 34.0 37.0 29.0 39.0 84-85 33.710347376752935 35.0 34.0 36.0 29.0 37.5 86-87 33.441659821681924 35.0 34.0 36.0 29.0 37.0 88-89 33.19874848788064 35.0 34.0 35.5 29.0 37.0 90-91 33.02644528741469 35.0 34.0 35.0 29.0 36.0 92-93 32.88853402828597 35.0 34.0 35.0 29.0 36.0 94-95 32.753087710389934 35.0 34.0 35.0 27.5 36.0 96-97 32.650965516211414 35.0 33.5 35.0 27.0 36.0 98-99 32.55524724084887 35.0 33.0 35.0 27.0 35.0 100-101 32.11836646305948 34.5 32.5 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39035431612402505 1101 2 0.2807734951151275 1101 3 0.26399383861338777 1101 4 0.25002669118391196 1101 5 0.33161676542617613 1101 6 0.31697081877980793 1101 7 0.4091838826900016 1101 8 0.29348559681613295 1101 9 0.3476306438176806 1101 10-11 0.23456523541185703 1101 12-13 0.30468706842716387 1101 14-15 0.40380258904112765 1101 16-17 0.47040578533523814 1101 18-19 0.42749531771888627 1101 20-21 0.2829291259903499 1101 22-23 0.4082391885469647 1101 24-25 0.2658758528095291 1101 26-27 0.30789671370636285 1101 28-29 0.34712349794355646 1101 30-31 0.34633174185824345 1101 32-33 0.3606368249291094 1101 34-35 0.36765688809108354 1101 36-37 0.4134980376986377 1101 38-39 0.4922815069718567 1101 40-41 0.47216781736483426 1101 42-43 0.4402747212829823 1101 44-45 0.720194206401743 1101 46-47 0.5501531997395475 1101 48-49 0.698145904037986 1101 50-51 0.619720072644462 1101 52-53 0.4427098787843988 1101 54-55 0.6917918596495909 1101 56-57 0.7829573578236477 1101 58-59 0.5919156738055804 1101 60-61 0.5913768523804208 1101 62-63 0.5175711106407874 1101 64-65 0.49808501765218693 1101 66-67 0.6985497302002983 1101 68-69 0.6028537829242495 1101 70-71 0.656742017399182 1101 72-73 0.37916367471122925 1101 74-75 0.35205663372342855 1101 76-77 0.48115520345624674 1101 78-79 0.6105958205233364 1101 80-81 0.49699617868004253 1101 82-83 0.4282619425675023 1101 84-85 0.5347658431726288 1101 86-87 0.34818119058597574 1101 88-89 0.3664743769887906 1101 90-91 0.49892063409281207 1101 92-93 0.07101720760722685 1101 94-95 0.27997914541342794 1101 96-97 0.38951781964411225 1101 98-99 0.200109927026908 1101 100-101 0.2577053375444578 1102 1 0.09695192997001811 1102 2 0.1928300310820248 1102 3 0.0826189417445562 1102 4 0.11131642565398181 1102 5 0.0020605307413532614 1102 6 -0.05424886374613891 1102 7 0.12920168150773748 1102 8 -0.008263075225194427 1102 9 0.014366322104045537 1102 10-11 0.061768379869654666 1102 12-13 -0.005512951967311608 1102 14-15 0.06092350666906299 1102 16-17 0.2245209894190836 1102 18-19 0.18287899657382667 1102 20-21 9.646432839431895E-4 1102 22-23 -0.08424884514718656 1102 24-25 0.09187541401922772 1102 26-27 0.08076865801089639 1102 28-29 -0.4521237603895969 1102 30-31 -0.16192802199841339 1102 32-33 0.025144560925674853 1102 34-35 0.11856305185875016 1102 36-37 0.08475449208761887 1102 38-39 -0.1420964768794306 1102 40-41 -0.069988497043866 1102 42-43 0.10024932088683869 1102 44-45 0.09805599981803681 1102 46-47 0.2733290526768144 1102 48-49 0.08560082760503462 1102 50-51 0.0865543242443465 1102 52-53 -0.04329827282691667 1102 54-55 -0.25745369914367444 1102 56-57 -0.3641934408055931 1102 58-59 0.01856971237587146 1102 60-61 0.2663722945234497 1102 62-63 0.037353754939445594 1102 64-65 0.16279051829028646 1102 66-67 4.065547799783076E-4 1102 68-69 -0.25008194047718035 1102 70-71 0.07927853433123033 1102 72-73 0.021836958253196315 1102 74-75 0.16134353768706688 1102 76-77 0.1643146172076868 1102 78-79 -0.15759157383480726 1102 80-81 -0.02080418409446594 1102 82-83 -0.1952621615432122 1102 84-85 -0.12250185290019999 1102 86-87 -0.05714207735113064 1102 88-89 -0.043014308710652926 1102 90-91 -0.10469722281829519 1102 92-93 0.08100277696853908 1102 94-95 0.005178995853917456 1102 96-97 0.013472988871932046 1102 98-99 0.10342464782340954 1102 100-101 0.022170739547441087 1103 1 0.13110896627851787 1103 2 0.1177951978716365 1103 3 0.12831191773013018 1103 4 0.1395313260719817 1103 5 0.09680686300919916 1103 6 0.10842222878725494 1103 7 0.12873040866124796 1103 8 0.07793887543458311 1103 9 0.31897250192537285 1103 10-11 0.10331210579376204 1103 12-13 0.08692824137749966 1103 14-15 0.036519769270491054 1103 16-17 0.18248550052472723 1103 18-19 0.08737452972685134 1103 20-21 0.18280424659514694 1103 22-23 -0.005997062506416739 1103 24-25 0.15097917600594712 1103 26-27 0.1381614946636276 1103 28-29 0.08371346606725183 1103 30-31 0.0358324074745795 1103 32-33 0.04420037602280047 1103 34-35 0.1963538067149102 1103 36-37 0.208566064909526 1103 38-39 0.24516686219114092 1103 40-41 0.11277316183655017 1103 42-43 0.11103737104665612 1103 44-45 -0.18129610246865724 1103 46-47 0.19819176884762157 1103 48-49 -0.09653588250068879 1103 50-51 -0.006296671575668711 1103 52-53 -0.11652997514639907 1103 54-55 -0.17136444416457408 1103 56-57 -0.10627400797221753 1103 58-59 -0.293361686689785 1103 60-61 -0.22627175104658193 1103 62-63 -0.2586260827787754 1103 64-65 -0.2055706816113556 1103 66-67 -0.2014826346307288 1103 68-69 -0.37650600407852863 1103 70-71 -0.3124823869047262 1103 72-73 -0.1937396338294164 1103 74-75 -0.28689840969429525 1103 76-77 -0.449231403610284 1103 78-79 -0.09435905191038074 1103 80-81 -0.02193114090128745 1103 82-83 -0.21468679886802988 1103 84-85 -0.3221678638829033 1103 86-87 -0.17107741052053882 1103 88-89 -0.20600299816234013 1103 90-91 -0.1451242369974537 1103 92-93 -0.2999188687982013 1103 94-95 -0.22621597177168695 1103 96-97 -0.3469790232582213 1103 98-99 -0.201704030153806 1103 100-101 -0.12701785155164202 1104 1 0.15942029080832754 1104 2 0.17393765485442714 1104 3 0.2065432102471405 1104 4 0.12170419521964249 1104 5 0.20781126476990153 1104 6 0.1815514804879399 1104 7 0.11482484643636326 1104 8 0.1604318726334668 1104 9 0.10212576322989975 1104 10-11 0.17329009699024311 1104 12-13 0.16206829739990525 1104 14-15 0.1757154475417977 1104 16-17 0.32631303153713276 1104 18-19 0.2546214284863524 1104 20-21 0.17885266596289284 1104 22-23 0.23932106474448034 1104 24-25 0.10365024443332516 1104 26-27 0.19084256709259506 1104 28-29 0.21450578299401712 1104 30-31 0.20036822180031066 1104 32-33 0.13591706269747306 1104 34-35 0.3286167118769754 1104 36-37 0.3193803906398145 1104 38-39 0.1930660218550031 1104 40-41 -0.05834528554240137 1104 42-43 -0.026327574931734432 1104 44-45 0.16273150857575303 1104 46-47 0.25087284127658904 1104 48-49 0.30040289300951173 1104 50-51 0.40303706191772903 1104 52-53 0.24389419452146655 1104 54-55 0.2379092653192174 1104 56-57 0.24809774072726043 1104 58-59 0.09364311523098223 1104 60-61 0.3204069204220019 1104 62-63 0.08681209249132849 1104 64-65 0.22969342403089854 1104 66-67 0.06440371991108407 1104 68-69 0.18912024642167324 1104 70-71 0.36043678076233476 1104 72-73 0.3411343157504092 1104 74-75 0.2048182769803688 1104 76-77 0.41468416261620433 1104 78-79 0.18419236865784683 1104 80-81 0.19713648614953172 1104 82-83 0.38735401745850595 1104 84-85 0.5181582665692801 1104 86-87 0.461365566670338 1104 88-89 0.4684567857512292 1104 90-91 0.21119829201415286 1104 92-93 0.5449290703774636 1104 94-95 0.2503546564796153 1104 96-97 0.12457960019115433 1104 98-99 0.28017872294743285 1104 100-101 0.3012534569717644 1105 1 0.10250661624759516 1105 2 0.10256637907138355 1105 3 -0.01748276377883684 1105 4 0.07552390492170247 1105 5 0.1571810126690636 1105 6 0.053304727241183514 1105 7 0.1675368403248214 1105 8 0.06873974123544713 1105 9 0.1808308816408939 1105 10-11 0.061993305546394595 1105 12-13 0.05824858769783958 1105 14-15 0.11247060414989818 1105 16-17 0.0790918703453869 1105 18-19 0.05417261259636774 1105 20-21 0.06526404869780578 1105 22-23 0.14393336420972247 1105 24-25 0.1393836286961161 1105 26-27 0.07384485891842019 1105 28-29 -0.030270763988411886 1105 30-31 -0.037876621591586 1105 32-33 0.12502134077234217 1105 34-35 0.1773371091267819 1105 36-37 0.09125777795837564 1105 38-39 0.055231797256077186 1105 40-41 0.00845188787241824 1105 42-43 0.021055923922354225 1105 44-45 -0.11183877408276288 1105 46-47 -0.1404755162482374 1105 48-49 -0.05154515893853784 1105 50-51 -0.11146828567585487 1105 52-53 -0.055962566364705424 1105 54-55 0.06576449832151354 1105 56-57 -0.13483770617877155 1105 58-59 -0.0018496273205812486 1105 60-61 0.15862311599114065 1105 62-63 0.004592841154433813 1105 64-65 -0.029550273448101905 1105 66-67 -0.13685678429058612 1105 68-69 -0.13350198428879168 1105 70-71 -0.003151836502752303 1105 72-73 0.16015647006668132 1105 74-75 0.15593554206822802 1105 76-77 0.11508523213492339 1105 78-79 0.14744676132400514 1105 80-81 0.003037937639220445 1105 82-83 0.3581332382377269 1105 84-85 0.21918958818883283 1105 86-87 0.15800422213251863 1105 88-89 0.2764877253998108 1105 90-91 0.13255046924867742 1105 92-93 -0.07225962328181623 1105 94-95 -0.19904373924231322 1105 96-97 0.01797150240659562 1105 98-99 0.05053777565942852 1105 100-101 0.06987454176931607 1106 1 0.11567139028193907 1106 2 0.13006967287902427 1106 3 0.12441760638240851 1106 4 0.12940118705985526 1106 5 0.2082794713762155 1106 6 -0.057924019857125586 1106 7 0.04935711344944593 1106 8 0.0983552545466182 1106 9 0.12164429528039733 1106 10-11 0.11746390177785315 1106 12-13 -0.019150663614453833 1106 14-15 0.007558909564288285 1106 16-17 -0.009128584722894573 1106 18-19 0.026273277631808867 1106 20-21 0.14333121100410295 1106 22-23 -0.12186514887140021 1106 24-25 -0.07993951997485738 1106 26-27 0.013913244638978028 1106 28-29 -0.04761684383448994 1106 30-31 -0.08490356807505606 1106 32-33 -0.23913131414118283 1106 34-35 -0.3200984263522315 1106 36-37 0.035076137537608076 1106 38-39 -0.03514139637798763 1106 40-41 -0.2376778881486601 1106 42-43 -0.13135064696248122 1106 44-45 -0.35371796976630776 1106 46-47 -0.3423205713541648 1106 48-49 -0.13739328826493136 1106 50-51 -0.20430268557547038 1106 52-53 -0.2234017093473213 1106 54-55 -0.24196732299726875 1106 56-57 -0.3555058263034212 1106 58-59 -0.5355196609906088 1106 60-61 -0.5197993069965037 1106 62-63 -0.6552953785163993 1106 64-65 -0.6049470988449244 1106 66-67 -0.3800704948956053 1106 68-69 -0.5627099542576346 1106 70-71 -0.46539983407683394 1106 72-73 -0.47422042517978014 1106 74-75 -0.47372676422451576 1106 76-77 -0.4231255506410818 1106 78-79 -0.32179012095635784 1106 80-81 -0.47307150042674095 1106 82-83 -0.39467956892291056 1106 84-85 -0.44321604160810324 1106 86-87 -0.26021523956519843 1106 88-89 -0.2050787154057616 1106 90-91 -0.3521882220117547 1106 92-93 -0.4961256123651978 1106 94-95 -0.39783679401362093 1106 96-97 -0.3702011140268979 1106 98-99 -0.4353668189408211 1106 100-101 -0.2668597786606526 1107 1 0.2600451561390784 1107 2 0.19177863740129197 1107 3 0.08135467329389456 1107 4 0.20147148675163606 1107 5 0.1247257228608305 1107 6 0.005511833860055049 1107 7 0.20575352560033622 1107 8 0.18077231650332237 1107 9 0.0690725419413809 1107 10-11 0.04088328811283759 1107 12-13 -0.06333571035704466 1107 14-15 0.09224205818605924 1107 16-17 0.1394567505632054 1107 18-19 -0.02027622288188269 1107 20-21 -0.10696935515324668 1107 22-23 0.11742923108793946 1107 24-25 0.22358129377613523 1107 26-27 0.17498391593982632 1107 28-29 0.22303842682077857 1107 30-31 0.11036606709227925 1107 32-33 0.2146070002109397 1107 34-35 0.2536113251068812 1107 36-37 0.30942563950859636 1107 38-39 0.4501873319174905 1107 40-41 0.19267820077520526 1107 42-43 0.3713453403882383 1107 44-45 0.2080019244344058 1107 46-47 0.20032339072714223 1107 48-49 0.2363434230676944 1107 50-51 0.3675490205473366 1107 52-53 0.2173266444245101 1107 54-55 0.47609069173594065 1107 56-57 0.29468252848852217 1107 58-59 0.25449207019759257 1107 60-61 0.4017256017406794 1107 62-63 0.4206841028339312 1107 64-65 0.32818512840495373 1107 66-67 0.32311951392100013 1107 68-69 0.3326238016899339 1107 70-71 0.2702622494113385 1107 72-73 0.3173032448605184 1107 74-75 0.2846071155927419 1107 76-77 0.14110088315057112 1107 78-79 0.2897946095542068 1107 80-81 0.41308132562377864 1107 82-83 0.262704157514527 1107 84-85 0.29365923618789935 1107 86-87 0.15768101592696837 1107 88-89 0.15658434446702074 1107 90-91 0.22966413989176004 1107 92-93 0.4131471268308218 1107 94-95 0.4340866107998025 1107 96-97 0.03694762432388643 1107 98-99 0.21792920775673963 1107 100-101 0.3504885356186094 1108 1 0.12659232282588562 1108 2 0.11521137502498391 1108 3 0.13532659303646 1108 4 0.13058383015967223 1108 5 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-1.118409501776604 2316 74-75 -0.8427207386258644 2316 76-77 -0.7078848649948384 2316 78-79 -0.885675578521024 2316 80-81 -1.0194301405011288 2316 82-83 -1.3305331374034424 2316 84-85 -1.2780001968160803 2316 86-87 -0.8942166662228175 2316 88-89 -0.7868853511035212 2316 90-91 -0.3991258376377118 2316 92-93 -0.6138944590342987 2316 94-95 -0.6264760758133079 2316 96-97 -0.5730194394246837 2316 98-99 -0.4853674955399683 2316 100-101 -0.9010274553931801 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 10.0 14 27.0 15 100.0 16 192.0 17 447.0 18 807.0 19 1211.0 20 1884.0 21 2829.0 22 3407.0 23 3708.0 24 3510.0 25 3430.0 26 3726.0 27 4459.0 28 5655.0 29 6870.0 30 8618.0 31 10963.0 32 13964.0 33 18341.0 34 25642.0 35 38265.0 36 66512.0 37 138471.0 38 222907.0 39 83463.0 40 171.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.939228946338226 20.421775497335886 15.13224935974635 22.506746196579535 2 14.73345898461419 20.959021951139377 41.45118268521367 22.85633637903276 3 17.983921432166394 26.07562572152685 31.302429706669933 24.63802313963682 4 11.939598518341498 18.482046839526824 40.523509379854225 29.05484526227745 5 12.38922325602234 38.41768843620723 36.317597335683026 12.875490972087396 6 28.852581430427577 39.29031198195911 18.91022864738123 12.946877940232081 7 25.96215594617602 33.57382876088353 22.891321554981406 17.57269373795905 8 24.335936916620618 36.91019877835691 21.000014934512166 17.753849370510313 9 25.058020579757763 17.111814692573066 21.382487791036304 36.44767693663287 10-11 23.588539255365223 27.391911468211894 29.572798279544198 19.446750996878688 12-13 24.424050538389164 24.698472199405607 29.528368105855822 21.349109156349407 14-15 21.603219880822593 26.47157215609552 27.387057751758537 24.538150211323348 16-17 21.026000985677804 29.693693155513074 27.950014187786554 21.330291671022568 18-19 20.76009199659493 28.78134380740453 29.874400752699415 20.584163443301122 20-21 21.82678952792007 27.784913155811765 30.005077734135817 20.38321958213235 22-23 21.301605033595635 27.672230842870395 29.714776852987168 21.311387270546806 24-25 21.220896369420092 27.6913484371033 29.92099643065159 21.166758762825015 26-27 21.223733926731285 27.96442599202497 29.59803760510163 21.213802476142117 28-29 21.186416625338346 27.952261306532662 29.652871969837296 21.208450098291696 30-31 21.5705458790386 27.669851066864936 29.760262539369332 20.999340514727134 32-33 21.337550842717814 27.90635157809374 29.56490506496891 21.191192514219534 34-35 21.455368210397406 28.013037829119313 29.517465911975986 21.014128048507295 36-37 21.49113636703057 27.875192281844114 29.467808659030155 21.165862692095164 38-39 21.397272958078826 28.019310324228258 29.480278976687224 21.10313774100569 40-41 21.617183649696084 27.815304888065835 29.39574963783808 21.171761824400008 42-43 21.432966442151166 27.84532325751579 29.336608969668003 21.385101330665034 44-45 21.620170552128915 28.034842216878985 29.11244194208396 21.23254528890814 46-47 21.445884795173164 27.982496751743604 28.89977448886632 21.671843964216908 48-49 21.49553164598565 28.063533988472813 28.974459719080812 21.466474646460725 50-51 21.598361818787406 28.283270328278494 28.739938732500605 21.378429120433502 52-53 21.623904180169955 28.32240624859989 28.73646559835123 21.317223972878928 54-55 21.610523556808076 28.238076356421566 28.68148632694802 21.469913759822337 56-57 21.628235188697563 28.39439059723114 28.48661120984483 21.49076300422647 58-59 21.63846532953001 28.329052106512943 28.64125808330471 21.39122448065234 60-61 21.67199330933855 28.347570901596498 28.66194238265207 21.31849340641288 62-63 21.582236891231947 28.352573963171494 28.616317447990564 21.448871697606 64-65 21.624352215534877 28.1666392867277 28.65925417046252 21.5497543272749 66-67 21.57574037844054 28.19374542630565 28.54978419629923 21.680729998954583 68-69 21.663704655087443 28.193446736062366 28.445018593467648 21.697830015382547 70-71 21.596872713152827 27.95942293044998 28.532833524992906 21.910870831404292 72-73 21.51794381636524 28.13699428008184 28.64760525097448 21.697456652578442 74-75 21.561253901641305 28.1741812153706 28.3942412521095 21.870323630878595 76-77 21.516450365148824 28.049552711360683 28.481533475709014 21.95246344778148 78-79 21.597817166812153 28.13250227565139 28.352189441724285 21.917491115812172 80-81 21.57432812903746 28.13172599105915 28.292589666189205 22.00135621371419 82-83 21.600742579433 28.095624277508445 28.379766950734663 21.92386619232389 84-85 21.68140205200197 28.128257590465804 28.266327155423472 21.924013202108753 86-87 21.491360384713033 28.14237070446094 28.43919413372362 21.927074777102405 88-89 21.62651771979868 28.06321778999089 28.34294120282561 21.96732328738482 90-91 21.68969070625308 28.09749249540764 28.276258606012632 21.936558192326647 92-93 21.653623859376633 28.066951418031934 28.274765154796217 22.00465956779522 94-95 21.593139085111783 28.11175495452441 28.21159216834182 22.083513792021982 96-97 21.47605250974477 27.985259636493975 28.41014650756433 22.128541346196926 98-99 21.48075688107648 27.951059603638047 28.217491300646664 22.35069221463881 100-101 21.576113741244644 27.962335160321988 28.189190400095583 22.27236069833779 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9.0 1 7.0 2 12.5 3 33.5 4 39.5 5 31.5 6 50.5 7 105.0 8 180.5 9 248.5 10 281.0 11 289.0 12 287.5 13 322.5 14 371.0 15 405.5 16 454.0 17 500.0 18 553.5 19 666.0 20 781.0 21 879.5 22 1104.0 23 1430.5 24 1814.5 25 2347.0 26 3075.5 27 4094.5 28 5324.5 29 6775.0 30 8720.0 31 11089.5 32 13903.5 33 16838.0 34 19564.0 35 21540.0 36 23452.0 37 25060.0 38 25706.5 39 26128.5 40 26252.5 41 25988.0 42 25361.0 43 25004.5 44 25188.0 45 25840.5 46 26247.5 47 26084.0 48 25819.0 49 25001.5 50 23664.5 51 22335.0 52 20798.0 53 19435.0 54 17968.0 55 16128.5 56 14212.5 57 12126.5 58 10329.0 59 8597.0 60 6707.0 61 5011.5 62 3622.0 63 2523.5 64 1651.5 65 1046.0 66 672.0 67 461.0 68 336.5 69 228.0 70 150.0 71 100.5 72 73.0 73 47.5 74 31.5 75 22.0 76 20.0 77 15.5 78 5.5 79 3.5 80 3.5 81 3.0 82 1.0 83 0.0 84 1.0 85 1.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04838781941187891 2 0.001792141459699219 3 4.4803536492480473E-4 4 0.010454158514912111 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001344106094774414 24-25 0.0 26-27 0.0 28-29 0.022999148732806642 30-31 0.019190848130945803 32-33 7.467256082080079E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.032258546274585936 50-51 0.010006123149987306 52-53 0.0 54-55 9.707432906704103E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.2401768246240237E-4 80-81 0.011798264609686525 82-83 0.0047790438925312505 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 669590.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.54701951230005 #Duplication Level Percentage of deduplicated Percentage of total 1 79.64884634866249 57.78286410188607 2 13.302074168698988 19.300516685413363 3 3.850922005347325 8.381187415868343 4 1.4477198198290027 4.201110320699128 5 0.706109929542918 2.5613085418189447 6 0.34922128599771285 1.5200978069631954 7 0.20894667342570383 1.0610920875831307 8 0.131580342068527 0.7636609314784437 9 0.08697831658825252 0.567901586760747 >10 0.25994192027429697 2.842230821797578 >50 0.005382176747793085 0.25499979458866284 >100 0.0016560093220239502 0.21129365816778428 >500 2.0700116498325135E-4 0.10497104971021036 >1k 4.140023299665027E-4 0.44676519726448616 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1535 0.22924476171985844 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1440 0.2150569751639063 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 699 0.10439224002747949 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.4934512164160158E-4 0.0 14-15 0.0 0.0 0.0 2.9869024328320315E-4 0.0 16-17 0.0 0.0 0.0 8.960707298496095E-4 0.0 18-19 0.0 0.0 0.0 0.0012694335339536135 0.0 20-21 0.0 0.0 0.0 0.002090831702982422 0.0 22-23 0.0 0.0 0.0 0.003509610358577637 0.0 24-25 0.0 0.0 0.0 0.005525769500739258 0.0 26-27 0.0 0.0 0.0 0.0062724951089472665 0.0 28-29 0.0 0.0 0.0 0.007616601203721681 0.0 30-31 0.0 0.0 0.0 0.009184724980958497 0.0 32-33 0.0 0.0 0.0 0.01329171582610254 0.0 34-35 0.0 0.0 0.0 0.020684299347361816 0.0 36-37 0.0 0.0 0.0 0.03248256395704834 0.0 38-39 0.0 0.0 0.0 0.049283890141728515 0.0 40-41 0.0 0.0 0.0 0.06981884436744874 0.0 42-43 0.0 0.0 0.0 0.09192192237040578 0.0 44-45 0.0 0.0 0.0 0.11581714183306202 0.0 46-47 0.0 0.0 0.0 0.14329664421511673 0.0 48-49 0.0 0.0 0.0 0.17443510207739066 0.0 50-51 0.0 0.0 0.0 0.21147269224450782 0.0 52-53 0.0 0.0 0.0 0.24664346839110501 0.0 54-55 0.0 0.0 0.0 0.286742633551875 0.0 56-57 0.0 0.0 0.0 0.3355038157678579 0.0 58-59 0.0 0.0 0.0 0.3891187144371929 0.0 60-61 0.0 0.0 0.0 0.45266506369569437 0.0 62-63 0.0 0.0 0.0 0.5159127227109126 1.4934512164160158E-4 64-65 0.0 0.0 0.0 0.5804298152600845 1.4934512164160158E-4 66-67 0.0 0.0 0.0 0.6534595797428278 2.2401768246240237E-4 68-69 0.0 0.0 0.0 0.7420959094371182 2.9869024328320315E-4 70-71 0.0 0.0 0.0 0.8386475305784136 2.9869024328320315E-4 72-73 0.0 0.0 0.0 0.941994354754402 2.9869024328320315E-4 74-75 0.0 0.0 0.0 1.0501202228229216 2.9869024328320315E-4 76-77 0.0 0.0 0.0 1.1750474170761211 2.9869024328320315E-4 78-79 0.0 0.0 0.0 1.3161785570274347 2.9869024328320315E-4 80-81 0.0 0.0 0.0 1.4773219432787228 2.9869024328320315E-4 82-83 0.0 0.0 0.0 1.6483967801191775 2.9869024328320315E-4 84-85 0.0 0.0 0.0 1.8113323078301646 2.9869024328320315E-4 86-87 0.0 0.0 0.0 1.9895010379485953 2.9869024328320315E-4 88-89 0.0 0.0 0.0 2.1955973058140055 2.9869024328320315E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 900 0.0 44.34735 1 GTATCAA 1695 0.0 41.207714 1 TCAACGC 1955 0.0 35.228157 4 CAACGCA 1965 0.0 35.04626 5 ATCAACG 2005 0.0 34.34965 3 AACGCAG 2050 0.0 33.824802 6 TATCAAC 2105 0.0 32.94594 2 CTTATCG 60 0.0039523393 31.662483 9 ACGCAGA 2325 0.0 29.824018 7 CGCAGAG 2370 0.0 29.257742 8 TAACGTC 70 0.008394519 27.13927 5 GCAGAGT 2655 0.0 25.401543 9 GTATAGG 245 1.0913936E-11 25.212048 1 GGCCGAC 95 0.0012269642 24.996698 7 GTGGTAT 540 0.0 24.637415 1 TAGCCCT 600 0.0 23.748638 4 TGGTATC 600 0.0 22.95873 2 TACATGG 1800 0.0 21.111475 2 AGAGTAC 2430 0.0 21.108324 10-11 GTAGCCC 475 0.0 20.998796 3 >>END_MODULE