FastQCFastQC Report
Fri 10 Feb 2017
SRR2031609_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031609_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032077
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGG45400.025.6395283
GTACATG48700.025.3298151
TACATGG48000.024.680732
GGTATCA36500.023.0962581
GTATCAA49750.022.497591
TCAACGC48800.021.6149544
CAACGCA49250.021.4174585
ATCAACG50250.021.3684063
AACGCAG50650.020.911546
CATGGGG24750.020.7333624
TATCAAC54700.019.5701922
GTGTACG1951.0095768E-519.5396421
ACGCAGA55600.019.048877
CGCAGAG55400.018.9471158
GAGTACA37350.017.8524841
TCTATAC11450.017.8427893
GTATAGA10150.017.3618851
CGTACAC1650.001461938717.2769533
CACCGTA1650.001465080417.2714149
CTAATAC10500.017.194683
Produced by FastQC (version 0.11.5)