##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031609_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2032077 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.135843277592336 34.0 31.0 34.0 30.0 34.0 2 32.36236471354186 34.0 31.0 34.0 31.0 34.0 3 32.51147864967715 34.0 31.0 34.0 31.0 34.0 4 35.59950877845672 37.0 35.0 37.0 33.0 37.0 5 35.350955204945485 37.0 35.0 37.0 33.0 37.0 6 35.56150775782611 37.0 35.0 37.0 33.0 37.0 7 35.69940066247489 37.0 36.0 37.0 33.0 37.0 8 35.77214101630992 37.0 36.0 37.0 35.0 37.0 9 37.72070595750063 39.0 38.0 39.0 35.0 39.0 10-11 37.679614010689555 39.0 38.0 39.0 35.0 39.0 12-13 37.70926520008838 39.0 38.0 39.0 35.0 39.0 14-15 39.18675547235661 41.0 39.0 41.0 36.0 41.0 16-17 39.1615745860024 41.0 39.0 41.0 36.0 41.0 18-19 39.094423341241495 41.0 39.0 41.0 36.0 41.0 20-21 39.10381988477799 41.0 39.0 41.0 36.0 41.0 22-23 39.0509685410543 41.0 39.0 41.0 36.0 41.0 24-25 38.99557103397164 41.0 39.0 41.0 36.0 41.0 26-27 38.92545484250843 41.0 39.0 41.0 35.0 41.0 28-29 38.83139320015925 41.0 39.0 41.0 35.0 41.0 30-31 38.72821256281135 40.0 38.5 41.0 35.0 41.0 32-33 38.60471871882808 40.0 38.0 41.0 34.5 41.0 34-35 38.50240049958737 40.0 38.0 41.0 34.0 41.0 36-37 38.394516792424696 40.0 38.0 41.0 34.0 41.0 38-39 38.24515089733312 40.0 38.0 41.0 34.0 41.0 40-41 38.13773543030112 40.0 38.0 41.0 33.0 41.0 42-43 38.002418953612484 40.0 38.0 41.0 33.0 41.0 44-45 37.82621869151612 40.0 38.0 41.0 33.0 41.0 46-47 37.67590007662111 40.0 38.0 41.0 32.5 41.0 48-49 37.82116524127777 40.0 38.0 41.0 33.0 41.0 50-51 37.60740980779764 39.5 37.5 40.5 32.5 41.0 52-53 37.75203006578983 40.0 38.0 41.0 33.0 41.0 54-55 38.055451392836 40.0 38.0 41.0 33.0 41.0 56-57 37.98737990735587 40.0 38.0 41.0 33.0 41.0 58-59 37.84237728196324 40.0 37.5 41.0 33.0 41.0 60-61 37.62139672856885 40.0 37.0 41.0 32.0 41.0 62-63 37.361676009324455 40.0 36.5 41.0 32.0 41.0 64-65 37.14687706223731 39.5 36.0 41.0 31.0 41.0 66-67 36.97192281591691 39.0 36.0 41.0 31.0 41.0 68-69 36.76587870439949 39.0 35.0 41.0 31.0 41.0 70-71 36.49752519220482 39.0 35.0 41.0 31.0 41.0 72-73 36.15300576700588 37.5 35.0 40.0 31.0 41.0 74-75 35.74116851871263 37.0 35.0 39.5 30.0 41.0 76-77 35.32548397526275 36.5 35.0 39.0 30.0 41.0 78-79 34.92642232553196 36.0 35.0 39.0 29.0 40.5 80-81 34.53455331663121 36.0 34.0 38.0 29.0 39.0 82-83 34.14805196850317 35.0 34.0 37.0 29.0 39.0 84-85 33.80778754938912 35.0 34.0 37.0 29.0 39.0 86-87 33.498766532961106 35.0 34.0 36.0 29.0 37.0 88-89 33.195980024379 35.0 34.0 36.0 28.0 37.0 90-91 32.94922707161195 35.0 34.0 36.0 27.0 37.0 92-93 32.737470578132616 35.0 34.0 35.0 26.5 36.0 94-95 32.557701799685745 35.0 34.0 35.0 26.0 36.0 96-97 32.42931837720716 35.0 33.5 35.0 26.0 36.0 98-99 32.31216090728846 35.0 33.0 35.0 25.0 36.0 100-101 31.853989046674904 34.5 32.5 35.0 24.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24435665597523837 1101 2 0.25307635709022236 1101 3 0.22546604461269482 1101 4 0.15823699981659445 1101 5 0.2055481859335515 1101 6 0.3033265386475179 1101 7 0.28403652825610237 1101 8 0.29449869729497635 1101 9 0.3099927440118577 1101 10-11 0.3964979464293208 1101 12-13 0.30818134350671045 1101 14-15 0.36699471700319464 1101 16-17 0.3205511294072849 1101 18-19 0.3821992613144545 1101 20-21 0.22321109566911446 1101 22-23 0.34074748387845233 1101 24-25 0.3904943702624095 1101 26-27 0.25626210165762586 1101 28-29 0.34026717496352177 1101 30-31 0.26292957654563764 1101 32-33 0.2631411948740521 1101 34-35 0.20787905934072626 1101 36-37 0.2856706704073346 1101 38-39 0.35549811498206196 1101 40-41 0.2944008993238256 1101 42-43 0.3680985511315811 1101 44-45 0.08400849810827538 1101 46-47 0.3244398136026163 1101 48-49 0.20505016970590617 1101 50-51 0.18092665098361493 1101 52-53 0.21393626043682445 1101 54-55 0.1805948529934085 1101 56-57 0.25202966639475477 1101 58-59 0.39904974397557424 1101 60-61 0.24645033241382208 1101 62-63 0.10758744244376572 1101 64-65 0.16602830381489753 1101 66-67 0.16038821490302269 1101 68-69 0.24729458804297622 1101 70-71 0.20855772651401594 1101 72-73 0.21799459012029132 1101 74-75 0.22143616722427595 1101 76-77 0.17212996414041015 1101 78-79 0.198516918046586 1101 80-81 0.26856667747992446 1101 82-83 0.30601249292173094 1101 84-85 0.1364061261870262 1101 86-87 0.14658843015598677 1101 88-89 0.2871159618458847 1101 90-91 0.34584417036095516 1101 92-93 0.24532685780119579 1101 94-95 0.09939948461194348 1101 96-97 0.231852349791275 1101 98-99 0.16793442935823322 1101 100-101 0.1763774796785924 1102 1 -0.12052171952753099 1102 2 -0.029128407422568614 1102 3 0.05824657632088304 1102 4 0.014266246906366575 1102 5 0.07857521088637043 1102 6 0.09005755542343508 1102 7 0.05152146691186488 1102 8 0.13025765997214478 1102 9 0.15235462790955978 1102 10-11 0.19582031675524547 1102 12-13 0.25062729883372725 1102 14-15 0.16195514063947059 1102 16-17 0.18142425131783568 1102 18-19 0.2848691396725158 1102 20-21 0.19303904575649966 1102 22-23 -1.5658050421052394E-4 1102 24-25 0.2477403855906246 1102 26-27 0.22076459763719214 1102 28-29 0.12538286911097174 1102 30-31 0.20826448432870137 1102 32-33 0.0785599298955546 1102 34-35 -0.01815221104485687 1102 36-37 0.013241389828529293 1102 38-39 -0.07429264832372695 1102 40-41 0.1969868806746149 1102 42-43 0.005957555321195684 1102 44-45 -0.2846196026797685 1102 46-47 -0.8123838212831842 1102 48-49 -0.2693942088457604 1102 50-51 -0.15395741067806767 1102 52-53 -0.08073793661125706 1102 54-55 -0.12318853574799249 1102 56-57 -0.15676887962302288 1102 58-59 -0.008720060776376215 1102 60-61 -0.3167105091376996 1102 62-63 -0.6854811996107841 1102 64-65 -0.4202078775979885 1102 66-67 -0.1506572359954248 1102 68-69 -0.09488676903036009 1102 70-71 -0.17934104590358402 1102 72-73 -0.26524467158604637 1102 74-75 -0.3648246076980186 1102 76-77 -0.2699940080776386 1102 78-79 -0.1457718062654081 1102 80-81 -0.14244354016391014 1102 82-83 -0.46643021815511787 1102 84-85 -0.5085213316813224 1102 86-87 -0.3036398883107765 1102 88-89 -0.3748661354746403 1102 90-91 -0.15251800656579206 1102 92-93 -0.3040530718020662 1102 94-95 -0.24983829101849864 1102 96-97 -0.2509152746850276 1102 98-99 -0.31414265821210563 1102 100-101 -0.30977271166239717 1103 1 -0.09097527932790683 1103 2 -0.053007777474803675 1103 3 -0.07839939847343658 1103 4 -0.03678787345171486 1103 5 -0.07376702707232141 1103 6 0.07530310688664343 1103 7 0.1498391464877571 1103 8 -0.03059197980803674 1103 9 0.13847532258684936 1103 10-11 0.13169309734156087 1103 12-13 0.09944339577267414 1103 14-15 0.12882430284519586 1103 16-17 0.1589598687404319 1103 18-19 0.15288401451797284 1103 20-21 -0.10863550712428349 1103 22-23 0.015366207526000153 1103 24-25 0.13582788696611914 1103 26-27 -0.032091592771188004 1103 28-29 -0.132203107884159 1103 30-31 0.03831646213941298 1103 32-33 0.009946630566808778 1103 34-35 0.05755156074472012 1103 36-37 0.1349662375523124 1103 38-39 0.08458274086255813 1103 40-41 0.0025503835777342942 1103 42-43 -0.04028327735944259 1103 44-45 0.006769751902119481 1103 46-47 0.24171549970406403 1103 48-49 0.0842801627923464 1103 50-51 0.10127305880519089 1103 52-53 -0.04026644673172086 1103 54-55 -0.04808513032754291 1103 56-57 -0.01097921175023231 1103 58-59 0.023000967905275616 1103 60-61 0.12084167904398413 1103 62-63 0.14860180953549218 1103 64-65 0.15533763049644023 1103 66-67 -0.1265736442833969 1103 68-69 -0.06667796452000374 1103 70-71 -0.031236326473717213 1103 72-73 0.04113571795625148 1103 74-75 -0.06355299612696541 1103 76-77 -0.5267287917839312 1103 78-79 -0.3065868157482825 1103 80-81 -0.07892024724337432 1103 82-83 -0.35358529068455624 1103 84-85 -0.3474098609617329 1103 86-87 -0.2481595628507094 1103 88-89 -0.07018392509073834 1103 90-91 -0.2737272836017013 1103 92-93 -0.3635689596646259 1103 94-95 -0.5117572625957649 1103 96-97 -0.35376984685520796 1103 98-99 -0.17982856465204122 1103 100-101 -0.13430099522951622 1104 1 0.01458395193293427 1104 2 0.10239693854201448 1104 3 0.18020284730251035 1104 4 0.1433809071453993 1104 5 0.11693551809451463 1104 6 0.11504422734594044 1104 7 0.09530218207227392 1104 8 0.16375233035667236 1104 9 0.2728078148548292 1104 10-11 0.2937716700801545 1104 12-13 0.2919976513734994 1104 14-15 0.2581917762126764 1104 16-17 0.2668055312656179 1104 18-19 0.38438302833702664 1104 20-21 0.3164055081131636 1104 22-23 0.39891610840714975 1104 24-25 0.27054674558764447 1104 26-27 0.29333638347033997 1104 28-29 0.32937886907219394 1104 30-31 0.38185405062498035 1104 32-33 0.2955999981353159 1104 34-35 0.2524819146494224 1104 36-37 0.329400997191442 1104 38-39 0.3279705400108952 1104 40-41 0.4388263815421496 1104 42-43 0.25180937294786787 1104 44-45 0.24008855281124397 1104 46-47 0.5830901251524736 1104 48-49 0.5262626149683598 1104 50-51 0.472027332308123 1104 52-53 0.4277329047862324 1104 54-55 0.43255336158865276 1104 56-57 0.44679496752251424 1104 58-59 0.3895678063111916 1104 60-61 0.42950270132960355 1104 62-63 0.553251162899997 1104 64-65 0.6626599692632098 1104 66-67 0.43149444883511734 1104 68-69 0.2722374680381705 1104 70-71 0.2711888461470693 1104 72-73 0.26436611624065165 1104 74-75 0.24161648866155616 1104 76-77 0.294445711855019 1104 78-79 0.2751015786139277 1104 80-81 0.31435797972157786 1104 82-83 0.29959950103440747 1104 84-85 0.317243861519259 1104 86-87 0.2765444107292794 1104 88-89 0.3996895144993502 1104 90-91 0.5001280610859098 1104 92-93 0.24171344612864942 1104 94-95 0.1399100748784221 1104 96-97 0.33585309463681057 1104 98-99 0.29030229602008717 1104 100-101 0.411706333368727 1105 1 0.2787965610174652 1105 2 0.30952730522792393 1105 3 0.3479415147917422 1105 4 0.3682061097380682 1105 5 0.1723791737374185 1105 6 0.09753459099016482 1105 7 0.2827765599903529 1105 8 0.2877903428711335 1105 9 0.28601064178493374 1105 10-11 0.28729137221549905 1105 12-13 0.23461323321744487 1105 14-15 0.23738549586287405 1105 16-17 0.3385407682704553 1105 18-19 0.3487359475449523 1105 20-21 0.27136813551834393 1105 22-23 0.23557439714490158 1105 24-25 0.26966321494831647 1105 26-27 0.3230106254323246 1105 28-29 0.2586354001926239 1105 30-31 0.18958426148570595 1105 32-33 0.20125278515859435 1105 34-35 0.2115147927822818 1105 36-37 0.27872561674281116 1105 38-39 0.4165572497354333 1105 40-41 0.37051840940670644 1105 42-43 0.27522025194969757 1105 44-45 0.40228472512900026 1105 46-47 0.40693959593863127 1105 48-49 0.379150240848098 1105 50-51 0.32569025134127827 1105 52-53 0.2591084069943719 1105 54-55 0.3569073171781625 1105 56-57 0.42647667572467185 1105 58-59 0.17497733188452003 1105 60-61 0.27227686795462347 1105 62-63 0.37020624311685424 1105 64-65 0.4339420499495006 1105 66-67 0.33460779433315935 1105 68-69 0.2786921629847754 1105 70-71 0.3244830831601746 1105 72-73 0.26664124979743065 1105 74-75 0.27857238449232824 1105 76-77 0.278903667417552 1105 78-79 0.3031590039660941 1105 80-81 0.37057064277153984 1105 82-83 0.28122197174972996 1105 84-85 0.31238605276722353 1105 86-87 0.3528150436916775 1105 88-89 0.3892844579383663 1105 90-91 0.3694079935800616 1105 92-93 0.46299134142773113 1105 94-95 0.268790565056257 1105 96-97 0.32902616085760883 1105 98-99 0.2577051820055374 1105 100-101 0.2400568584738494 1106 1 0.05436335625417854 1106 2 0.008980918507070612 1106 3 0.04550098743856523 1106 4 0.1812894441512185 1106 5 0.06269063576461775 1106 6 -0.04726436893140118 1106 7 0.061225167244124634 1106 8 0.04502561166604124 1106 9 0.12730797550411665 1106 10-11 0.13966675069088552 1106 12-13 0.14774926263449828 1106 14-15 0.0872968789915447 1106 16-17 0.1651490097114774 1106 18-19 0.1341712500301071 1106 20-21 -0.019937908713785646 1106 22-23 0.1560174671039931 1106 24-25 0.05401380661530908 1106 26-27 -3.002909280311883E-4 1106 28-29 0.13580190632669087 1106 30-31 0.11802962929251493 1106 32-33 0.14118566381754505 1106 34-35 0.061911661028943854 1106 36-37 0.10021740366494925 1106 38-39 0.005417727474679168 1106 40-41 0.10049150040061505 1106 42-43 -0.02525328320837872 1106 44-45 0.10842729246990501 1106 46-47 0.42843210273786525 1106 48-49 0.23293917000211195 1106 50-51 0.02695075614789033 1106 52-53 0.05200825913161822 1106 54-55 0.1078394860382943 1106 56-57 0.0915584609622968 1106 58-59 0.10957678506586888 1106 60-61 0.29225964849432984 1106 62-63 0.34898772154285496 1106 64-65 0.45548811560517066 1106 66-67 0.38824252581179763 1106 68-69 0.21600713657247894 1106 70-71 0.05775709862025735 1106 72-73 0.1855351442317783 1106 74-75 0.28611223328934443 1106 76-77 0.2607009989771072 1106 78-79 0.24918290676540522 1106 80-81 0.2373343488716415 1106 82-83 0.09876177735412028 1106 84-85 0.08098832498730957 1106 86-87 0.14611054028449644 1106 88-89 0.211587978281365 1106 90-91 0.20148499739396897 1106 92-93 0.24949782104153684 1106 94-95 0.06376524697855501 1106 96-97 0.22523586393709394 1106 98-99 0.2864232392588093 1106 100-101 0.333686090091426 1107 1 0.07572252511697286 1107 2 -0.09718772801130626 1107 3 0.05301721474636878 1107 4 0.19887688203888132 1107 5 0.04678800762162183 1107 6 0.08131117916578035 1107 7 -0.006314343012924439 1107 8 0.1590917704867323 1107 9 0.2113388627927506 1107 10-11 0.19146623964083176 1107 12-13 0.17003027074348864 1107 14-15 0.11804332180013688 1107 16-17 0.17200840152452912 1107 18-19 0.24474777003021586 1107 20-21 0.09629967883090984 1107 22-23 0.25480993111822414 1107 24-25 0.17510677019831888 1107 26-27 0.22576139286708496 1107 28-29 0.2613418598141948 1107 30-31 0.20862406541525758 1107 32-33 0.2485185913013126 1107 34-35 0.19784870628383544 1107 36-37 0.2428640501993442 1107 38-39 0.23119838714043794 1107 40-41 0.28020329380182574 1107 42-43 0.3156297867232425 1107 44-45 0.13161211689991603 1107 46-47 0.29240334081936936 1107 48-49 0.2668317480280251 1107 50-51 0.01900638424016421 1107 52-53 0.09811116869925485 1107 54-55 0.19720262261468946 1107 56-57 0.25605049482937403 1107 58-59 0.21566853377022 1107 60-61 0.2559760290637456 1107 62-63 0.20483863056580276 1107 64-65 0.361583735900858 1107 66-67 0.15930077857598945 1107 68-69 0.2218177276443214 1107 70-71 0.17645341366633005 1107 72-73 0.2774281654881463 1107 74-75 0.3585693320522978 1107 76-77 0.32213754626526026 1107 78-79 0.363521145004448 1107 80-81 0.40098050678449937 1107 82-83 0.28494753897623326 1107 84-85 0.3107440409246536 1107 86-87 0.26034731613003714 1107 88-89 0.3078501571061665 1107 90-91 0.35807978596503176 1107 92-93 0.26690583069648 1107 94-95 0.18965270916378785 1107 96-97 0.25260173999811286 1107 98-99 0.19547488628715826 1107 100-101 0.21866271368998014 1108 1 0.05406468669132636 1108 2 0.0869996144459364 1108 3 0.037531293995073156 1108 4 0.020610391101818948 1108 5 0.13767996899451873 1108 6 0.18575219365344964 1108 7 0.03567534373068071 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-1.1149651349499834 2316 76-77 -0.7957471690996698 2316 78-79 -1.1146109816295109 2316 80-81 -1.1120174274415433 2316 82-83 -1.1330257800510353 2316 84-85 -1.633101488291267 2316 86-87 -1.1985452427018828 2316 88-89 -1.0071345795362774 2316 90-91 -1.2386187061739342 2316 92-93 -1.5027453720750437 2316 94-95 -1.2818649339985306 2316 96-97 -1.1584865869381495 2316 98-99 -0.8257073893485902 2316 100-101 -1.2804755273664625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 12.0 7 115.0 8 560.0 9 661.0 10 576.0 11 730.0 12 955.0 13 1293.0 14 1640.0 15 2104.0 16 2690.0 17 3474.0 18 4313.0 19 5607.0 20 6747.0 21 8081.0 22 8968.0 23 9506.0 24 9971.0 25 10730.0 26 12474.0 27 14529.0 28 17466.0 29 20879.0 30 25567.0 31 31505.0 32 40366.0 33 53239.0 34 75098.0 35 112845.0 36 189410.0 37 367427.0 38 654218.0 39 338037.0 40 283.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.72420322679367 21.84199063890673 14.439538283628172 21.994267850671427 2 13.650052398011542 21.00370094909047 44.18515412719531 21.16109252570268 3 17.57295564212157 26.849313177399463 31.854408695562142 23.723322484916824 4 11.030416968324452 17.463076100615282 42.3758337097891 29.130673221271163 5 11.387200633767765 39.573390632884795 37.767389274388194 11.272019458959246 6 28.448377447313938 40.76456234687724 18.946125935746746 11.84093427006207 7 25.184542822365113 33.957703421085974 24.375823004539065 16.481930752009845 8 23.039670511813004 39.56022556175981 21.434776306746638 15.96532761968055 9 24.421496118657753 17.350728793120958 22.386761729068212 35.841013359153074 10-11 22.92014739682678 28.061187384145153 30.971503066493995 18.04716215253407 12-13 23.1093821756239 25.420544713280712 31.132104075649607 20.33796903544578 14-15 20.286475847120844 27.735528290611605 27.949712153455046 24.028283708812502 16-17 20.127452969765507 31.31160128369047 28.566250130516412 19.994695616027613 18-19 20.09951608059744 30.38213879576435 30.106777333077346 19.411567790560863 20-21 20.710148129812524 29.366079596706605 30.158424738530037 19.76534753495083 22-23 20.509803516303762 29.206816473982038 29.91188326032921 20.371496749384992 24-25 20.525624767171717 29.351126950405913 29.84286520638736 20.280383076035015 26-27 20.574663263252326 29.3028020099632 29.78199152886431 20.34054319792016 28-29 20.47604495302097 29.415543800751642 29.830513312241614 20.27789793398577 30-31 20.711887394030835 29.35073326453673 29.594646265864927 20.34273307556751 32-33 20.555052785893448 29.296552246789858 29.747765463611863 20.40062950370483 34-35 20.624858211573677 29.396228587794653 29.65020518415395 20.328708016477723 36-37 20.904645837731543 29.045651321283593 29.591693621846023 20.45800921913884 38-39 20.621905567554773 29.390372510490497 29.608646719587888 20.37907520236684 40-41 20.597324806097404 29.156301655891976 29.77490518321894 20.471468354791675 42-43 20.678055014647576 29.104507358727055 29.522355698135456 20.695081928489913 44-45 20.74186103183245 29.22257136866143 29.42368168883389 20.611885910672225 46-47 20.811558667447162 29.105123492376133 29.42488251913302 20.658435321043687 48-49 20.667116516976513 29.1399814313613 29.486308707312446 20.70659334434974 50-51 20.550682881538254 29.21621582360775 29.510390027031626 20.72271126782237 52-53 20.675406782778573 29.243964279496907 29.33578449900362 20.7448444387209 54-55 20.7415769017951 29.14880418982355 29.335118165070835 20.774500743310515 56-57 20.907329848864574 29.170092754397174 29.152652346153673 20.769925050584572 58-59 20.905870397981648 28.842401790195794 29.360757651210317 20.890970160612245 60-61 20.85163810961404 28.968498906087103 29.439279394889617 20.740583589409237 62-63 20.600909817649217 29.071591840499984 29.57644863289329 20.75104970895751 64-65 20.85076114016293 29.11011253008391 29.27201373462296 20.767112595130204 66-67 20.748259943654148 29.003607454914203 29.206862391087917 21.041270210343736 68-69 20.739964741766816 28.988553759691765 29.229134424415626 21.042347074125793 70-71 20.783249436032825 28.78550037926177 29.361879759242658 21.06937042546275 72-73 20.742189693024578 28.855561891985857 29.25800729444604 21.144241120543526 74-75 20.71788088804083 29.05361868925741 29.05270700374062 21.175793418961135 76-77 20.710267966234643 29.02150935086782 29.162876451860843 21.1053462310367 78-79 20.765816865080733 29.00984004990987 29.086173369773583 21.138169715235815 80-81 20.87668446353284 28.919800819575244 28.969522045785883 21.233992671106034 82-83 20.794611925606628 28.949078727050676 29.015843525575402 21.240465821767295 84-85 20.852525992907644 28.862459625836117 28.991371246255675 21.293643135000565 86-87 20.64800984550651 28.978228291451025 29.132175136944237 21.241586726098223 88-89 20.963030263773295 28.70776724781892 28.936545306010665 21.39265718239712 90-91 20.92407055548042 28.78304039501531 28.91357734554503 21.379311703959242 92-93 21.088194780078066 28.718788332577105 28.894663772542973 21.298353114801856 94-95 20.89169466778819 28.623010047881547 29.04074785291884 21.444547431411422 96-97 20.928419845096712 28.480453858901523 29.194558559614663 21.3965677363871 98-99 20.96960906593711 28.5491570249562 28.83923943249243 21.64199447661426 100-101 20.9723730951844 28.5375674496522 28.88130338481781 21.608756070345592 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 41.0 1 30.5 2 39.0 3 68.5 4 71.5 5 74.0 6 115.0 7 207.0 8 320.5 9 371.5 10 369.5 11 375.5 12 392.0 13 447.0 14 531.5 15 599.5 16 661.0 17 765.5 18 841.0 19 953.0 20 1091.0 21 1210.5 22 1489.0 23 1821.0 24 2220.5 25 2902.0 26 4078.0 27 6126.5 28 10191.5 29 17638.5 30 28859.0 31 43370.0 32 61510.0 33 79861.5 34 96049.5 35 107794.0 36 116744.5 37 122552.5 38 119937.0 39 111655.5 40 98606.5 41 85302.0 42 75245.0 43 67315.5 44 60759.5 45 55705.0 46 53000.5 47 52104.0 48 52654.0 49 51395.5 50 48387.0 51 45882.0 52 43013.0 53 40397.5 54 38544.5 55 35886.5 56 32175.5 57 28425.5 58 25224.0 59 21851.0 60 18180.0 61 14624.5 62 11315.5 63 8502.5 64 6134.5 65 4406.5 66 3199.5 67 2402.0 68 1809.0 69 1329.5 70 1012.5 71 769.0 72 555.0 73 419.5 74 331.0 75 233.0 76 155.5 77 97.0 78 66.5 79 55.5 80 48.0 81 35.0 82 26.0 83 23.5 84 21.0 85 14.0 86 12.0 87 14.0 88 11.0 89 7.0 90 6.5 91 6.0 92 4.5 93 5.0 94 4.5 95 3.5 96 2.0 97 1.0 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46504143297719525 2 0.35387438566550383 3 0.18818184547140684 4 0.2161827529173353 5 0.23798310792356786 6 0.19113448949030967 7 0.18139076422793032 8 0.1830147184383269 9 0.17986523148483055 10-11 0.19421016034333344 12-13 0.18333458820704135 14-15 0.1925616007661127 16-17 0.08272324326292754 18-19 0.0367604180353402 20-21 3.9368586918704364E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.13988151039552144 46-47 0.21160615468803595 48-49 0.08720142002493016 50-51 0.06434303424525743 52-53 0.0727580696991305 54-55 0.08038573341462947 56-57 0.11357837326046209 58-59 0.0938940798011099 60-61 0.14239125781158884 62-63 0.2444543193983299 64-65 0.1354771497339914 66-67 0.10403149093267626 68-69 0.10809137645866765 70-71 0.08941590303910728 72-73 0.07337320386973524 74-75 0.1411609894703793 76-77 0.21541998654578542 78-79 0.13872505815478448 80-81 0.08646325902020445 82-83 0.08934208693863471 84-85 0.09861831023135442 86-87 0.07411136487446096 88-89 0.0956410608456274 90-91 0.11729378365091479 92-93 0.16576635629456954 94-95 0.13257371644873692 96-97 0.09067077674714097 98-99 0.07969678314355215 100-101 0.06412158594383972 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2032077.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.203914800908066 #Duplication Level Percentage of deduplicated Percentage of total 1 68.83718188145889 34.558960143113744 2 15.328323587870923 15.390837028924425 3 6.145082558988822 9.25521603708063 4 3.163336266389563 6.35247457617777 5 1.809981763198536 4.543408511540833 6 1.1245122257059479 3.387294958315254 7 0.762056207945862 2.678074345605199 8 0.5546797664033156 2.2277676587439714 9 0.40195154688468365 1.816158709250268 >10 1.7827048404700259 14.856796914910744 >50 0.062294707645971184 2.1237199593034792 >100 0.026712471629090184 2.3465108304597124 >500 8.86631556280776E-4 0.27676805680325256 >1k 2.9554385209358915E-4 0.18601226977073346 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 9.84214672967609E-5 0.0 4 0.0 0.0 0.0 9.84214672967609E-5 0.0 5 0.0 0.0 0.0 9.84214672967609E-5 0.0 6 0.0 0.0 0.0 1.4763220094514136E-4 0.0 7 0.0 0.0 0.0 1.4763220094514136E-4 0.0 8 0.0 0.0 0.0 1.4763220094514136E-4 0.0 9 0.0 0.0 0.0 1.968429345935218E-4 0.0 10-11 0.0 0.0 0.0 2.952644018902827E-4 0.0 12-13 0.0 0.0 0.0 4.4289660283542407E-4 0.0 14-15 0.0 0.0 0.0 5.413180701321849E-4 0.0 16-17 0.0 0.0 0.0 0.0012302683412095112 0.0 18-19 0.0 0.0 0.0 0.0020422454464077886 0.0 20-21 0.0 0.0 0.0 0.003690805023628534 0.0 22-23 0.0 0.0 0.0 0.006372790007465268 0.0 24-25 0.0 0.0 0.0 0.00984214672967609 0.0 26-27 0.0 0.0 0.0 0.011589127774193596 0.0 28-29 0.0 0.0 0.0 0.014935457662283467 0.0 30-31 0.0 0.0 0.0 0.02322746628203557 0.0 32-33 0.0 0.0 0.0 0.04205057190254109 0.0 34-35 0.0 0.0 0.0 0.07000226861482119 0.0 36-37 0.0 0.0 0.0 0.11343074105951693 0.0 38-39 0.0 0.0 0.0 0.1835314311416349 0.0 40-41 0.0 0.0 0.0 0.26490137922923196 0.0 42-43 0.0 0.0 0.0 0.3463697487841258 0.0 44-45 0.0 0.0 0.0 0.43802474020423443 0.0 46-47 0.0 0.0 0.0 0.5497331055860579 0.0 48-49 0.0 0.0 0.0 0.6633360842133442 0.0 50-51 0.0 0.0 0.0 0.7918991258697382 0.0 52-53 0.0 0.0 0.0 0.9205851943602531 2.4605366824190224E-5 54-55 0.0 0.0 0.0 1.0586951183444326 4.921073364838045E-5 56-57 0.0 0.0 0.0 1.2231819955641443 4.921073364838045E-5 58-59 0.0 0.0 0.0 1.3953949579666518 4.921073364838045E-5 60-61 0.0 0.0 0.0 1.5768595382950548 4.921073364838045E-5 62-63 0.0 0.0 0.0 1.761473605576954 4.921073364838045E-5 64-65 0.0 0.0 0.0 1.9511071676909881 4.921073364838045E-5 66-67 0.0 0.0 0.0 2.1696274304566217 4.921073364838045E-5 68-69 0.0 0.0 0.0 2.4169359724065576 4.921073364838045E-5 70-71 0.0 0.0 0.0 2.692516080837488 4.921073364838045E-5 72-73 0.0 0.0 0.0 2.962313928064734 4.921073364838045E-5 74-75 0.0 0.0 0.0 3.2363685037525647 4.921073364838045E-5 76-77 0.0 0.0 0.0 3.539211358624698 4.921073364838045E-5 78-79 0.0 0.0 0.0 3.883981758565251 4.921073364838045E-5 80-81 0.0 0.0 0.0 4.271663918247192 4.921073364838045E-5 82-83 0.0 0.0 0.0 4.6694834890607 4.921073364838045E-5 84-85 0.0 0.0 0.0 5.060954875233566 4.921073364838045E-5 86-87 0.0 0.0 0.0 5.472774899770038 4.921073364838045E-5 88-89 0.0 0.0 0.0 5.931517358840241 4.921073364838045E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATGGG 4540 0.0 25.639528 3 GTACATG 4870 0.0 25.329815 1 TACATGG 4800 0.0 24.68073 2 GGTATCA 3650 0.0 23.096258 1 GTATCAA 4975 0.0 22.49759 1 TCAACGC 4880 0.0 21.614954 4 CAACGCA 4925 0.0 21.417458 5 ATCAACG 5025 0.0 21.368406 3 AACGCAG 5065 0.0 20.91154 6 CATGGGG 2475 0.0 20.733362 4 TATCAAC 5470 0.0 19.570192 2 GTGTACG 195 1.0095768E-5 19.539642 1 ACGCAGA 5560 0.0 19.04887 7 CGCAGAG 5540 0.0 18.947115 8 GAGTACA 3735 0.0 17.852484 1 TCTATAC 1145 0.0 17.842789 3 GTATAGA 1015 0.0 17.361885 1 CGTACAC 165 0.0014619387 17.276953 3 CACCGTA 165 0.0014650804 17.271414 9 CTAATAC 1050 0.0 17.19468 3 >>END_MODULE