FastQCFastQC Report
Fri 10 Feb 2017
SRR2031609_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031609_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032077
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37650.025.4940071
TACACCG2101.2678356E-924.8783745
GTATCAA51050.022.8045771
ACATGGG44150.022.162423
TCAACGC51350.022.0149214
ATCAACG51950.021.669233
CAACGCA52250.021.5432225
TATACCG1553.9114435E-521.4493925
AACGCAG53350.021.1880596
GTACATG52150.020.5923461
TACATGG49200.020.3703272
CTAGACG1656.327227E-520.1509134
ACACCGT3153.2196112E-1019.6011436
TATCAAC58500.019.3242112
CGCAGAG58800.019.3049728
GAGTACA39250.018.6437261
ACGCAGA60500.018.605517
AGAGTAC50000.017.7631610-11
GGCCGGT2152.398028E-517.6725887
GTATAGG8350.017.6411511
Produced by FastQC (version 0.11.5)