FastQCFastQC Report
Fri 10 Feb 2017
SRR2031608_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031608_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194489
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32430.27149684928031986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27980.2342424250034952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16400.13729720407638749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23750.041.9278261
GTATCAA37150.035.9102861
GTACATG51350.029.8768671
TCAACGC45350.028.8287684
CAACGCA46300.028.4426125
ATCAACG46500.028.113443
AACGCAG47500.028.0113896
TACATGG55400.026.8825232
TATCAAC49300.026.7342682
ACATGGG52600.026.6606353
ACGCAGA53700.024.3348467
CGCAGAG54400.024.1964198
CATGGGA22400.023.1339174
CATGGGG28500.022.019114
CGTATGA1100.002849046621.6278212
CCTATAC4050.021.1276783
GCAGAGT60350.021.10229
TGGACCG2056.772916E-720.8717735
TCTATAC3252.1827873E-1120.4775923
GAGTACT31350.020.08392312-13
Produced by FastQC (version 0.11.5)