##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031608_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1194489 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95751656147524 33.0 31.0 34.0 30.0 34.0 2 32.19458195094304 34.0 31.0 34.0 30.0 34.0 3 32.33944222173666 34.0 31.0 34.0 30.0 34.0 4 35.43153348419282 37.0 35.0 37.0 33.0 37.0 5 35.14367231510713 37.0 35.0 37.0 32.0 37.0 6 35.368105524621825 37.0 35.0 37.0 33.0 37.0 7 35.513168392509264 37.0 35.0 37.0 33.0 37.0 8 35.5960674397169 37.0 35.0 37.0 33.0 37.0 9 37.531191999256585 39.0 38.0 39.0 35.0 39.0 10-11 37.48367921345446 39.0 38.0 39.0 35.0 39.0 12-13 37.50570788010606 39.0 38.0 39.0 35.0 39.0 14-15 38.916990026697604 41.0 39.0 41.0 35.5 41.0 16-17 38.86956723753839 41.0 39.0 41.0 35.0 41.0 18-19 38.8083653344652 41.0 39.0 41.0 35.0 41.0 20-21 38.808870990021674 41.0 39.0 41.0 35.0 41.0 22-23 38.743115256816935 41.0 39.0 41.0 34.5 41.0 24-25 38.668098659761625 41.0 38.0 41.0 34.0 41.0 26-27 38.558703345112434 40.0 38.0 41.0 34.0 41.0 28-29 38.444148083406375 40.0 38.0 41.0 34.0 41.0 30-31 38.29021531382876 40.0 38.0 41.0 34.0 41.0 32-33 38.121657880482786 40.0 38.0 41.0 33.5 41.0 34-35 37.98399859688955 40.0 38.0 41.0 33.0 41.0 36-37 37.84835481950859 40.0 38.0 41.0 33.0 41.0 38-39 37.66083027972631 40.0 37.0 41.0 32.5 41.0 40-41 37.505017208195305 40.0 37.0 41.0 32.0 41.0 42-43 37.308225107137865 40.0 37.0 41.0 31.0 41.0 44-45 37.07023170577544 40.0 36.5 41.0 30.5 41.0 46-47 36.841073881802174 40.0 36.0 41.0 30.0 41.0 48-49 36.943756284067916 40.0 37.0 41.0 30.0 41.0 50-51 36.685295971750264 39.5 36.5 40.5 30.0 41.0 52-53 36.79303492958077 39.5 36.0 40.5 30.5 41.0 54-55 37.04508580656666 40.0 36.0 41.0 31.0 41.0 56-57 36.92072970115254 40.0 36.0 41.0 30.5 41.0 58-59 36.705702605884184 39.5 35.5 41.0 30.0 41.0 60-61 36.41630354067723 39.0 35.0 41.0 29.0 41.0 62-63 36.080648712545695 39.0 35.0 41.0 28.5 41.0 64-65 35.784910116376125 38.0 35.0 41.0 28.0 41.0 66-67 35.54035407609446 37.5 35.0 40.0 28.0 41.0 68-69 35.270381728086235 37.0 35.0 40.0 28.0 41.0 70-71 34.95096271292578 36.5 34.5 39.0 28.0 41.0 72-73 34.58399365753891 36.0 34.0 39.0 27.5 41.0 74-75 34.161626017485304 35.5 34.0 38.5 26.0 40.5 76-77 33.76891875940255 35.0 34.0 37.0 26.0 39.5 78-79 33.428464807963906 35.0 34.0 37.0 26.0 39.0 80-81 33.121054275091694 35.0 34.0 36.5 26.0 39.0 82-83 32.812133891563676 35.0 33.0 36.0 25.0 37.5 84-85 32.55528347268162 35.0 33.0 36.0 25.0 37.0 86-87 32.33515921871194 35.0 33.0 35.0 25.0 37.0 88-89 32.09644542561715 35.0 33.0 35.0 24.0 36.0 90-91 31.914389751600893 35.0 33.0 35.0 24.0 36.0 92-93 31.74887587914162 35.0 32.0 35.0 23.5 36.0 94-95 31.5958434945822 35.0 32.0 35.0 23.0 36.0 96-97 31.504165798094416 35.0 32.0 35.0 22.5 35.0 98-99 31.38577458645496 35.0 32.0 35.0 20.0 35.0 100-101 30.91821858552067 34.5 31.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09817849598033845 1101 2 0.2161206003204157 1101 3 0.24407158431700537 1101 4 0.15413149366305134 1101 5 0.19845109399693683 1101 6 0.2262297071795487 1101 7 0.28226626950334577 1101 8 0.30098063095239525 1101 9 0.1933102114137739 1101 10-11 0.3019888589377686 1101 12-13 0.2653725284012083 1101 14-15 0.42762489636359646 1101 16-17 0.4344105196292958 1101 18-19 0.4932856602321678 1101 20-21 0.15129085565980205 1101 22-23 0.3119257668342996 1101 24-25 0.3339721215658571 1101 26-27 0.28337082045749185 1101 28-29 0.5402294182788268 1101 30-31 0.36100826072453884 1101 32-33 0.34147813173969155 1101 34-35 0.3824008379627841 1101 36-37 0.40830417362381866 1101 38-39 0.3275449324832209 1101 40-41 0.30489979117254506 1101 42-43 0.4144312520057696 1101 44-45 0.6548371808027298 1101 46-47 0.7381704465863095 1101 48-49 0.775493282754411 1101 50-51 0.6725198448830554 1101 52-53 0.32665091690483905 1101 54-55 0.25155837976868156 1101 56-57 0.4918987883077932 1101 58-59 0.5942591287512116 1101 60-61 0.38825637051031237 1101 62-63 0.35659544601309534 1101 64-65 0.43007205570210516 1101 66-67 0.24983358116211463 1101 68-69 0.2948164998155107 1101 70-71 0.2701890339233586 1101 72-73 0.3238530710961669 1101 74-75 0.2671732408644445 1101 76-77 0.1979041607526213 1101 78-79 0.17569968900209432 1101 80-81 0.17760957210194306 1101 82-83 0.26668683458939313 1101 84-85 0.04165678831084563 1101 86-87 0.07765747361493425 1101 88-89 0.029081703122571412 1101 90-91 -0.019212287890997715 1101 92-93 -0.12313577810676435 1101 94-95 -0.12720734560453195 1101 96-97 0.160781894935063 1101 98-99 0.10428735476743611 1101 100-101 0.10392401348813252 1102 1 0.27160014135844435 1102 2 0.21476354211880988 1102 3 0.22596315156933144 1102 4 0.20916929742723767 1102 5 0.35808031132011564 1102 6 0.3045633436358415 1102 7 0.28372026043363974 1102 8 0.33181123451352335 1102 9 0.3714355923993722 1102 10-11 0.30242705482981336 1102 12-13 0.3377298124460779 1102 14-15 0.3928994957915961 1102 16-17 0.45551537286115007 1102 18-19 0.3899173978062862 1102 20-21 0.43356745569937516 1102 22-23 0.13394428598201102 1102 24-25 0.36567462003192475 1102 26-27 0.267748162582059 1102 28-29 0.19335165144900657 1102 30-31 0.42288032151435573 1102 32-33 0.32357055959476355 1102 34-35 0.1657556896956791 1102 36-37 0.29452813419518264 1102 38-39 0.11887524924338777 1102 40-41 0.2108795412260136 1102 42-43 -0.11190547030894749 1102 44-45 -0.22178344448332155 1102 46-47 -0.7968997147217394 1102 48-49 0.07314671127502237 1102 50-51 0.1337647409252014 1102 52-53 0.12064588439739765 1102 54-55 0.039116314561937315 1102 56-57 0.12794537197871847 1102 58-59 0.14060546439427668 1102 60-61 -0.11358335272176845 1102 62-63 -0.580046524459398 1102 64-65 -0.2107936834748969 1102 66-67 0.010884911538830977 1102 68-69 -0.10313282335421547 1102 70-71 -0.028354776672081528 1102 72-73 0.012382231858595105 1102 74-75 -0.09316568392174673 1102 76-77 -0.06863785908561937 1102 78-79 -0.18797192289924425 1102 80-81 -0.03579732351250442 1102 82-83 -0.22159929483679264 1102 84-85 -0.057122658752902566 1102 86-87 -0.07959089601655478 1102 88-89 0.05002566801093877 1102 90-91 0.09662382184884066 1102 92-93 0.05464384283629897 1102 94-95 -0.16122123996184712 1102 96-97 -0.041589659206366036 1102 98-99 -0.11708251454325591 1102 100-101 1.1905412992518905E-4 1103 1 0.19091203938901558 1103 2 0.09079593466277203 1103 3 -0.04151013416549176 1103 4 0.047628255690689514 1103 5 0.10186870558912631 1103 6 0.26475884893078216 1103 7 0.28497475274281214 1103 8 0.15352957271851153 1103 9 0.3874545679132879 1103 10-11 0.2182184200126258 1103 12-13 0.17697414614678308 1103 14-15 0.33531216530139574 1103 16-17 0.2875633866615246 1103 18-19 0.28326159254489625 1103 20-21 0.19600216747281962 1103 22-23 0.1905382770692725 1103 24-25 0.3070723870547738 1103 26-27 0.23831263620321863 1103 28-29 0.0808675841721822 1103 30-31 0.3152354894668221 1103 32-33 0.33623336087896405 1103 34-35 0.4312264836963564 1103 36-37 0.2005102128764733 1103 38-39 0.2201196787800228 1103 40-41 0.14279721816309632 1103 42-43 0.30255521300122723 1103 44-45 0.19602766290083906 1103 46-47 0.37812975482638933 1103 48-49 0.3653600420527283 1103 50-51 0.38272156683923697 1103 52-53 0.2423038459671929 1103 54-55 0.2830518169817893 1103 56-57 0.15560074597843965 1103 58-59 0.13983638106493856 1103 60-61 0.362063779388059 1103 62-63 0.5849839034480269 1103 64-65 0.6220900196253893 1103 66-67 0.10876070817499084 1103 68-69 0.10463611622122926 1103 70-71 0.05574891780297264 1103 72-73 0.12561621527898126 1103 74-75 0.1724512598704777 1103 76-77 -0.21556679674434776 1103 78-79 -0.07860396085027332 1103 80-81 0.20327019289940296 1103 82-83 0.05450114121669003 1103 84-85 -0.05880074586775663 1103 86-87 -0.04243482720634972 1103 88-89 0.28096965191050316 1103 90-91 0.1333783381169944 1103 92-93 0.13001113403377218 1103 94-95 -0.3018916122844324 1103 96-97 -0.048893315347051214 1103 98-99 0.13840888418212316 1103 100-101 0.16489168338863536 1104 1 0.1747655131510264 1104 2 0.3344147447145076 1104 3 0.2216227187539488 1104 4 0.1913089652250548 1104 5 -0.034566068985888876 1104 6 0.11795822069722561 1104 7 0.09146680743608471 1104 8 0.3047453894221377 1104 9 0.41052367360434516 1104 10-11 0.4089034717495039 1104 12-13 0.2841674203522544 1104 14-15 0.35055283418535765 1104 16-17 0.3007900126777798 1104 18-19 0.3871788135352858 1104 20-21 0.4100057705770368 1104 22-23 0.34489341380245975 1104 24-25 0.3371901808463562 1104 26-27 0.4822349384944218 1104 28-29 0.4362149603732135 1104 30-31 0.5427036158526306 1104 32-33 0.4205289078117076 1104 34-35 0.4199612397939134 1104 36-37 0.5057702830908184 1104 38-39 0.4213604400512736 1104 40-41 0.3257596165370842 1104 42-43 0.32691265941659964 1104 44-45 0.39432080776410317 1104 46-47 0.45761839119003156 1104 48-49 0.48071018987239 1104 50-51 0.3654839467357647 1104 52-53 0.4043414475932039 1104 54-55 0.36890362777262453 1104 56-57 0.3078258937980962 1104 58-59 0.2919913145460882 1104 60-61 0.4073792819749045 1104 62-63 0.48305347107551455 1104 64-65 0.3826614349986315 1104 66-67 0.4302250466820112 1104 68-69 0.38944025517171355 1104 70-71 0.3226109835827202 1104 72-73 0.34280209850145127 1104 74-75 0.10424773067608584 1104 76-77 0.21763349889497619 1104 78-79 0.17111656760501148 1104 80-81 0.3054066489230536 1104 82-83 0.32630546494173984 1104 84-85 0.2395681565712664 1104 86-87 0.24200392002202875 1104 88-89 0.3857627043864795 1104 90-91 0.19911143124337727 1104 92-93 0.2320053136346587 1104 94-95 0.21538712016198502 1104 96-97 0.18433331066921 1104 98-99 0.3469177533617227 1104 100-101 0.45431195315951456 1105 1 0.019566126865342426 1105 2 0.07412386822274186 1105 3 0.09845199136783123 1105 4 0.06383213896432238 1105 5 -0.19697270078564344 1105 6 -0.08455598827745803 1105 7 0.18022949275513156 1105 8 0.14463044200596897 1105 9 0.29010349898632626 1105 10-11 0.2763671343341869 1105 12-13 0.2645636416862658 1105 14-15 0.2961632000044432 1105 16-17 0.29479239028925974 1105 18-19 0.18058528233932236 1105 20-21 0.21457434571097878 1105 22-23 0.3212289156875343 1105 24-25 0.1592825675288907 1105 26-27 0.0657246237000848 1105 28-29 0.05636052263758273 1105 30-31 0.0834264132843856 1105 32-33 0.10453380122667966 1105 34-35 0.20210284409700563 1105 36-37 0.11915905268573823 1105 38-39 0.22392035869514615 1105 40-41 0.14212323518378867 1105 42-43 0.28171102308131424 1105 44-45 0.214615812299634 1105 46-47 0.14993518045782395 1105 48-49 0.1138325146165613 1105 50-51 -0.032783692235810236 1105 52-53 0.13042404035903132 1105 54-55 0.23692847398970684 1105 56-57 0.38729117925320367 1105 58-59 0.39194799666191926 1105 60-61 0.31351190733986556 1105 62-63 0.19123045253652293 1105 64-65 0.24684303703396182 1105 66-67 0.16950542058001616 1105 68-69 0.1197886408054103 1105 70-71 0.2438650053249276 1105 72-73 0.18327712472249402 1105 74-75 0.1753931476616586 1105 76-77 0.4232490557733968 1105 78-79 0.32247979647934955 1105 80-81 0.1508596884388922 1105 82-83 0.15701226398928014 1105 84-85 0.2893664493682735 1105 86-87 0.45974272646814995 1105 88-89 0.405176911943812 1105 90-91 0.4147906450370691 1105 92-93 0.15110584301640628 1105 94-95 0.27496161274922315 1105 96-97 0.25410743757909415 1105 98-99 0.03918006308193611 1105 100-101 0.2272992217764589 1106 1 0.2432199311127512 1106 2 0.07160087752176736 1106 3 0.1728474885958562 1106 4 0.2024209710226259 1106 5 0.22933484225226408 1106 6 -0.03388488932236555 1106 7 0.09451740866538216 1106 8 0.1893341915264415 1106 9 0.17360678550278408 1106 10-11 0.20084064127468793 1106 12-13 0.23454419518650838 1106 14-15 0.13154323531803414 1106 16-17 0.21412131619121766 1106 18-19 0.27265000015793106 1106 20-21 0.1082491375953154 1106 22-23 0.17962078047180796 1106 24-25 0.21200271607463606 1106 26-27 0.10888102178078896 1106 28-29 0.20260449790978896 1106 30-31 0.2394321668631747 1106 32-33 0.3294810007069984 1106 34-35 0.31316042048479176 1106 36-37 0.18629214810947303 1106 38-39 0.15981982352283097 1106 40-41 0.31424961506084514 1106 42-43 0.058506302892993745 1106 44-45 0.12852134390496417 1106 46-47 0.3236213403002637 1106 48-49 0.2621346537315361 1106 50-51 -0.028950320650238837 1106 52-53 0.15799051018547772 1106 54-55 0.21775250180971284 1106 56-57 0.18032929919842644 1106 58-59 0.11428603433029139 1106 60-61 0.30534015174433904 1106 62-63 0.42132471876051625 1106 64-65 0.5756599822515014 1106 66-67 0.357882097705982 1106 68-69 0.4780276347245547 1106 70-71 0.41165507261296597 1106 72-73 0.3203281338773536 1106 74-75 0.2577855725581557 1106 76-77 0.29371316177712004 1106 78-79 0.2459569499492673 1106 80-81 0.23022908162026567 1106 82-83 0.4295872307844064 1106 84-85 0.3884139271647129 1106 86-87 0.3067094619336217 1106 88-89 0.34600843002193216 1106 90-91 0.32808593603442304 1106 92-93 0.3419288682686812 1106 94-95 0.1899401403997949 1106 96-97 0.22963981347405138 1106 98-99 0.21716193566303588 1106 100-101 0.26470286963158784 1107 1 -0.0938598725598716 1107 2 -0.05711890114354645 1107 3 -0.017599628741898243 1107 4 0.16852235236011381 1107 5 0.05635043944040774 1107 6 -0.028614391851235155 1107 7 -0.038243315991401516 1107 8 0.0462498597125105 1107 9 0.183315231480762 1107 10-11 0.11765589480512517 1107 12-13 0.1824435893529639 1107 14-15 0.01840753985827348 1107 16-17 0.03549138343496594 1107 18-19 0.08652211601174287 1107 20-21 0.10588138375845801 1107 22-23 0.1639900591066734 1107 24-25 0.23589532959229587 1107 26-27 0.10108705298132747 1107 28-29 0.08868944411401714 1107 30-31 0.05442205678941292 1107 32-33 0.15656790417217792 1107 34-35 0.12479968626910676 1107 36-37 0.2396038043345854 1107 38-39 0.35636073675195235 1107 40-41 0.3541402736302075 1107 42-43 0.2585046069599173 1107 44-45 0.2887954991953876 1107 46-47 0.461471791379914 1107 48-49 0.2659161990503307 1107 50-51 0.3271848328818123 1107 52-53 0.431987770482678 1107 54-55 0.19352879590302052 1107 56-57 0.21043018409005043 1107 58-59 0.3837027335665866 1107 60-61 0.26277107523386434 1107 62-63 0.342116935352756 1107 64-65 0.48688867805584124 1107 66-67 0.28705954703805503 1107 68-69 0.2177890169408414 1107 70-71 0.38579710392452427 1107 72-73 0.4336172341925675 1107 74-75 0.34904681485996747 1107 76-77 0.2146664920115242 1107 78-79 0.261099705115889 1107 80-81 0.4442835873675506 1107 82-83 0.41098631390131146 1107 84-85 0.4801985960838806 1107 86-87 0.3056672339709223 1107 88-89 0.41382069948103606 1107 90-91 0.4281186212900572 1107 92-93 0.17816031996427384 1107 94-95 0.006189646073845978 1107 96-97 0.3132905561000179 1107 98-99 0.29005506644028856 1107 100-101 0.443404494104211 1108 1 0.3511919611755765 1108 2 0.322641154380797 1108 3 0.37849604057979747 1108 4 0.3346921643044567 1108 5 0.2498197192764806 1108 6 0.3302975954574876 1108 7 0.2699454227026763 1108 8 0.3353840545544742 1108 9 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-1.4266335954403928 2316 68-69 -1.5212392793610263 2316 70-71 -1.2492128825358648 2316 72-73 -1.0553545995405713 2316 74-75 -1.190081745276089 2316 76-77 -0.9062991433862919 2316 78-79 -1.3372044787034199 2316 80-81 -1.2290237047446837 2316 82-83 -1.2215059552894019 2316 84-85 -1.4922265681993778 2316 86-87 -1.267321187210186 2316 88-89 -1.168235667272441 2316 90-91 -1.3068514991748295 2316 92-93 -1.58538157126943 2316 94-95 -1.4602348018762221 2316 96-97 -1.3954572638174696 2316 98-99 -0.9021067313278976 2316 100-101 -1.2230500101829556 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 77.0 8 371.0 9 371.0 10 424.0 11 505.0 12 731.0 13 942.0 14 1234.0 15 1610.0 16 2182.0 17 2908.0 18 3793.0 19 4983.0 20 6435.0 21 8091.0 22 9398.0 23 9426.0 24 9457.0 25 9400.0 26 9979.0 27 11568.0 28 13519.0 29 16091.0 30 19321.0 31 23382.0 32 29580.0 33 38426.0 34 54074.0 35 82740.0 36 141686.0 37 256399.0 38 324025.0 39 101228.0 40 128.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.96019803861719 19.070718587898554 12.827944701886798 23.141138671597453 2 15.523831124103287 19.631150983653377 41.032206038002116 23.812811854241218 3 18.96980193660126 25.34624400840396 29.449758658721205 26.234195396273574 4 12.22338553123233 15.936130979646007 38.23181504729019 33.608668441831476 5 12.982012151887687 38.53395303876907 34.81806991237249 13.665964896970756 6 31.80506747406295 37.01185094480463 17.42088886279575 13.762192718336674 7 28.592322858913597 32.33906157093652 21.503576409534745 17.565039160615132 8 26.157986502772157 36.33198115050994 19.516971334115514 17.99306101260239 9 26.57431036781781 15.999936263586967 20.234801591062144 37.19095177753308 10-11 25.09187348948157 26.54561970099756 27.99659807031074 20.365908739210134 12-13 25.31754488147968 24.135633418749983 28.343231516856264 22.20359018291407 14-15 22.766833824406373 25.711510735250826 25.80716723406361 25.71448820627919 16-17 22.14203308234896 29.041855212149798 26.533910538746465 22.282201166754778 18-19 22.059336472966617 27.952953235878248 28.499018288845424 21.488692002309712 20-21 22.68161980833613 27.17011570657529 28.61659440412495 21.53167008096363 22-23 22.686604899668396 26.832017707990612 28.332324533754598 22.14905285858639 24-25 22.312428159656555 27.129801948783122 28.38083900312184 22.176930888438488 26-27 22.056419104738513 27.337003522008157 28.452250292803033 22.1543270804503 28-29 22.17031718165676 26.95600378069618 28.759703940346036 22.113975097301022 30-31 22.41175933809353 27.233026005262502 28.305367399783503 22.049847256860463 32-33 22.069730236109333 27.431227914195944 28.41574932879248 22.08329252090224 34-35 22.22444074411736 27.339808068554838 28.43592532036712 21.999825866960684 36-37 22.48291947435263 27.009541318505235 28.192976243397805 22.31456296374433 38-39 22.274880723053958 27.464087153586174 28.33818478027006 21.922847343089806 40-41 22.304767980282783 27.259229678967323 28.57920834767001 21.856793993079886 42-43 22.318958148630923 27.04101921407397 28.23048182109672 22.409540816198394 44-45 22.252516154396506 27.402181917554213 28.115293163998402 22.230008764050876 46-47 22.427617365540957 27.266939262380994 27.950511672697242 22.35493169938081 48-49 22.214690960153526 27.543864132308542 27.802882760452384 22.43856214708555 50-51 22.28875871991355 27.47863346310673 27.62849765130398 22.60411016567574 52-53 22.40073052781053 27.623920596988665 27.622203186424915 22.353145688775886 54-55 22.384232524021915 27.57326804885109 27.669703417255565 22.37279600987143 56-57 22.49945313698227 27.724404505983646 27.320228735409653 22.45591362162443 58-59 22.371424453486892 27.443345980841038 27.62053119532355 22.56469837034852 60-61 22.51011446573992 27.769846233294153 27.56634133827837 22.153697962687556 62-63 22.300013090235353 27.76409549830163 27.780710027791578 22.15518138367144 64-65 22.37588049051799 27.495949093963418 27.718340272754023 22.409830142764577 66-67 22.367311193565488 27.711192727440338 27.422066997555 22.499429081439175 68-69 22.317124477043553 27.584695478438103 27.43019704730745 22.6679829972109 70-71 22.334074327930747 27.246382519119084 27.80472599818508 22.61481715476509 72-73 22.276980329389215 27.57483767002183 27.508862645024625 22.639319355564332 74-75 22.19090102763326 27.724468871716024 27.30609688826144 22.778533212389277 76-77 22.151286491420592 27.608807272946933 27.702353505760435 22.53755272987204 78-79 22.24427494354899 27.57324169833805 27.350916734187113 22.831566623925838 80-81 22.321550509963412 27.74954190011537 27.135013577519214 22.793894012402003 82-83 22.512661932171678 27.49569626277662 27.372690446394643 22.618951358657057 84-85 22.345394247549375 27.492515612986484 27.25661862831979 22.905471511144352 86-87 22.251260541866493 27.641147806257116 27.422445834504476 22.685145817371914 88-89 22.357837349554078 27.43128906482404 27.346570926511415 22.864302659110468 90-91 22.302185105810548 27.324173618310937 27.52117761538416 22.852463660494347 92-93 22.58974743845285 27.435702420294145 27.4078207793275 22.566729361925503 94-95 22.306389438772367 27.237611275435807 27.620282673871337 22.835716611920486 96-97 22.52994018247738 27.11072735392718 27.46029830836817 22.899034155227266 98-99 22.355969620931898 27.146034844606792 27.473661280930976 23.02433425353033 100-101 22.497255507727928 27.079856034050554 27.409613953287714 23.013274504933804 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 103.0 1 78.5 2 100.5 3 160.5 4 141.0 5 143.5 6 266.5 7 538.5 8 864.5 9 1000.5 10 892.5 11 799.0 12 826.5 13 874.0 14 937.0 15 971.0 16 1032.5 17 1087.5 18 1113.5 19 1183.5 20 1335.0 21 1529.0 22 1765.0 23 2128.5 24 2494.0 25 2971.5 26 3923.5 27 5259.0 28 6653.5 29 9074.5 30 12522.0 31 16176.5 32 21196.0 33 26197.0 34 30318.0 35 34526.0 36 40623.5 37 45249.0 38 44320.5 39 42857.0 40 40521.5 41 37359.0 42 35880.0 43 36363.5 44 38100.5 45 39010.5 46 38850.5 47 38442.5 48 39140.5 49 39757.5 50 39681.5 51 39158.5 52 38705.5 53 38785.5 54 38118.0 55 36425.5 56 34091.0 57 30862.5 58 27958.5 59 25061.0 60 21704.0 61 18424.5 62 14709.5 63 11146.5 64 8254.0 65 6085.0 66 4400.0 67 3225.0 68 2482.0 69 1837.0 70 1402.0 71 1113.0 72 825.5 73 625.0 74 487.0 75 351.5 76 260.5 77 180.5 78 113.5 79 85.0 80 66.5 81 47.0 82 36.5 83 25.0 84 24.5 85 24.5 86 18.0 87 14.5 88 11.0 89 9.0 90 12.5 91 12.0 92 6.5 93 3.5 94 4.0 95 4.0 96 2.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4375092612824396 2 0.3372990458681495 3 0.18367686935585006 4 0.2082061869133998 5 0.22938679217640345 6 0.18242110224539532 7 0.17513765300475767 8 0.17547252423421228 9 0.17396560370166658 10-11 0.18623026248044142 12-13 0.17568181875262143 14-15 0.18434661181475928 16-17 0.07718781838928612 18-19 0.033528981849142186 20-21 1.6743561472730182E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.12955330689524977 46-47 0.19836934454817082 48-49 0.07915518686233193 50-51 0.062076754160147136 52-53 0.06961135682287573 54-55 0.07898775124760463 56-57 0.11050750572001919 58-59 0.09250817713683425 60-61 0.13754835749847844 62-63 0.23114486613104013 64-65 0.12942773018420428 66-67 0.10351706880515435 68-69 0.1065727687739276 70-71 0.08773626211710614 72-73 0.07162058419960335 74-75 0.13821809995738762 76-77 0.21473617588776456 78-79 0.13733906298006932 80-81 0.08137370875746867 82-83 0.08786183882815161 84-85 0.09807541132651704 86-87 0.07162058419960335 88-89 0.09376394424728901 90-91 0.11435852485874713 92-93 0.16308228874439196 94-95 0.13055792058361357 96-97 0.08589447035510583 98-99 0.07568089785674041 100-101 0.061490729508601585 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1194489.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.890474725176844 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2732446618678 42.07057378256175 2 13.798328812766744 15.423902955192382 3 4.758780274607932 7.979114659819341 4 2.1224232389676922 4.744929695746071 5 1.1439079555811542 3.1966779339668614 6 0.7049648645374701 2.3640492566141513 7 0.44724390982658196 1.74976721067063 8 0.3079660922277546 1.3769896875093441 9 0.2256793122095242 1.1351991505537535 >10 1.0885219822387617 10.9134000993292 >50 0.07818177590247948 3.035861380704525 >100 0.048793306348010136 4.784123461204741 >500 0.0015106253216429434 0.5769072269276105 >1k 4.5318759649288305E-4 0.648503499199616 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3243 0.27149684928031986 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2798 0.2342424250034952 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1640 0.13729720407638749 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.2557671104547634E-4 0.0 12-13 0.0 4.185890368182545E-5 0.0 6.697424589092072E-4 0.0 14-15 0.0 1.2557671104547634E-4 0.0 9.627547846819853E-4 0.0 16-17 0.0 1.674356147273018E-4 0.0 0.0020929451840912727 0.0 18-19 0.0 1.674356147273018E-4 0.0 0.0038928780424097665 0.0 20-21 0.0 1.674356147273018E-4 0.0 0.006781142396455723 0.0 22-23 0.0 1.674356147273018E-4 0.0 0.012934401237684065 0.0 24-25 0.0 2.0929451840912724E-4 0.0 0.02013413267095804 0.0 26-27 0.0 2.511534220909527E-4 0.0 0.02327355044709495 0.0 28-29 0.0 2.511534220909527E-4 0.0 0.025826943571686304 0.0 30-31 0.0 2.511534220909527E-4 0.0 0.03168719008714187 0.0 32-33 0.0 2.511534220909527E-4 0.0 0.043742554347507595 0.0 34-35 0.0 3.348712294546036E-4 0.0 0.061825600738056194 0.0 36-37 0.0 3.7673013313642905E-4 0.0 0.08953619497542464 0.0 38-39 0.0 4.185890368182545E-4 0.0 0.1286324110142496 0.0 40-41 0.0 4.185890368182545E-4 0.0 0.1781096351661673 0.0 42-43 0.0 4.185890368182545E-4 0.0 0.22553577303767552 0.0 44-45 0.0 5.023068441819054E-4 0.0 0.2784872861951847 0.0 46-47 0.0 5.023068441819054E-4 0.0 0.3469265937149693 0.0 48-49 0.0 5.023068441819054E-4 0.0 0.4121427656512534 0.0 50-51 0.0 5.023068441819054E-4 0.0 0.48983289088472143 0.0 52-53 0.0 5.023068441819054E-4 0.0 0.5650951997046436 0.0 54-55 0.0 5.023068441819054E-4 0.0 0.6420737235755206 0.0 56-57 0.0 5.023068441819054E-4 0.0 0.7370934349332643 0.0 58-59 0.0 5.023068441819054E-4 0.0 0.8439592160329648 0.0 60-61 0.0 5.023068441819054E-4 0.0 0.9594060723874394 0.0 62-63 0.0 5.023068441819054E-4 0.0 1.0716716520620952 0.0 64-65 0.0 5.023068441819054E-4 0.0 1.1832674892778419 0.0 66-67 0.0 5.023068441819054E-4 0.0 1.3193089262437745 0.0 68-69 0.0 5.023068441819054E-4 0.0 1.4721776424898012 0.0 70-71 0.0 5.441657478637309E-4 0.0 1.6392783859876483 0.0 72-73 0.0 5.860246515455563E-4 0.0 1.8128254006524966 0.0 74-75 0.0 5.860246515455563E-4 0.0 1.9790471071730256 0.0 76-77 0.0 5.860246515455563E-4 0.0 2.160003147789557 0.0 78-79 0.0 5.860246515455563E-4 0.0 2.3862086632861415 0.0 80-81 0.0 5.860246515455563E-4 0.0 2.636692342918185 0.0 82-83 0.0 5.860246515455563E-4 0.0 2.8985616443516853 0.0 84-85 0.0 5.860246515455563E-4 0.0 3.1545706992697298 0.0 86-87 0.0 5.860246515455563E-4 0.0 3.4262768430684583 0.0 88-89 0.0 5.860246515455563E-4 0.0 3.7316794043310573 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2375 0.0 41.927826 1 GTATCAA 3715 0.0 35.910286 1 GTACATG 5135 0.0 29.876867 1 TCAACGC 4535 0.0 28.828768 4 CAACGCA 4630 0.0 28.442612 5 ATCAACG 4650 0.0 28.11344 3 AACGCAG 4750 0.0 28.011389 6 TACATGG 5540 0.0 26.882523 2 TATCAAC 4930 0.0 26.734268 2 ACATGGG 5260 0.0 26.660635 3 ACGCAGA 5370 0.0 24.334846 7 CGCAGAG 5440 0.0 24.196419 8 CATGGGA 2240 0.0 23.133917 4 CATGGGG 2850 0.0 22.01911 4 CGTATGA 110 0.0028490466 21.627821 2 CCTATAC 405 0.0 21.127678 3 GCAGAGT 6035 0.0 21.1022 9 TGGACCG 205 6.772916E-7 20.871773 5 TCTATAC 325 2.1827873E-11 20.477592 3 GAGTACT 3135 0.0 20.083923 12-13 >>END_MODULE