FastQCFastQC Report
Fri 10 Feb 2017
SRR2031608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194489
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34370.28773810390886817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32620.2730874876202293No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18540.15521281485220878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24800.041.203491
GTATCAA41000.037.4425931
TCAACGC48650.031.3463944
CAACGCA49100.030.954575
ATCAACG49550.030.7770333
AACGCAG51100.029.8359876
GTACATG47750.029.0640931
TATCAAC54150.028.1637292
CGTATAC856.401137E-427.9457953
ACATGGG49200.026.6507453
TACATGG51800.026.23132
ACGCAGA58050.026.1820747
CGCAGAG59900.025.3734478
GGACCGT2251.0186341E-1025.3311566
GTGTAGC6200.024.530451
TCGCACG500.001652978223.75293222-23
TACGCTG1000.001654510723.747965
GCAGAGT64450.023.287379
CATGGGG28000.022.9055714
CCGATCG550.002879700921.58905418-19
Produced by FastQC (version 0.11.5)