##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031608_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1194489 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.42167320084153 34.0 31.0 34.0 31.0 34.0 2 32.647196416208104 34.0 31.0 34.0 31.0 34.0 3 32.75209148012246 34.0 31.0 34.0 31.0 34.0 4 36.21786889623931 37.0 35.0 37.0 35.0 37.0 5 35.873591133949326 37.0 35.0 37.0 35.0 37.0 6 35.94528455264134 37.0 36.0 37.0 35.0 37.0 7 35.9176015852804 37.0 36.0 37.0 35.0 37.0 8 36.03950559611683 37.0 36.0 37.0 35.0 37.0 9 37.933759122101584 39.0 38.0 39.0 35.0 39.0 10-11 37.90797027013225 39.0 38.0 39.0 35.0 39.0 12-13 37.918319884067586 39.0 38.0 39.0 35.0 39.0 14-15 39.358776012169216 41.0 39.0 41.0 36.0 41.0 16-17 39.321919247477375 41.0 39.0 41.0 36.0 41.0 18-19 39.34281186348305 41.0 39.0 41.0 36.0 41.0 20-21 39.36438175655029 41.0 39.0 41.0 36.0 41.0 22-23 39.29834180138955 41.0 39.0 41.0 36.0 41.0 24-25 39.2230313548304 41.0 39.0 41.0 36.0 41.0 26-27 39.10429899312593 41.0 39.0 41.0 36.0 41.0 28-29 39.01340154660277 40.0 39.0 41.0 35.0 41.0 30-31 38.88528693022707 40.0 38.0 41.0 35.0 41.0 32-33 38.71880486132564 40.0 38.0 41.0 35.0 41.0 34-35 38.595386395354 40.0 38.0 41.0 35.0 41.0 36-37 38.44964332028172 40.0 38.0 41.0 34.0 41.0 38-39 38.312527783847315 40.0 38.0 41.0 34.0 41.0 40-41 38.18213520593325 40.0 38.0 41.0 33.5 41.0 42-43 38.00833787502438 40.0 38.0 41.0 33.0 41.0 44-45 37.90326825948167 40.0 37.5 41.0 33.0 41.0 46-47 37.85624438567454 40.0 37.5 41.0 33.0 41.0 48-49 37.930679143968675 40.0 38.0 41.0 33.0 41.0 50-51 37.82577570827358 40.0 37.5 41.0 33.0 41.0 52-53 37.71994635362904 40.0 37.0 41.0 33.0 41.0 54-55 37.589467546373385 40.0 37.0 41.0 33.0 41.0 56-57 37.45658436369025 40.0 36.5 41.0 32.5 41.0 58-59 37.24180214300843 39.5 36.0 41.0 32.0 41.0 60-61 36.94349968898834 39.0 35.5 41.0 31.0 41.0 62-63 36.731447087415624 39.0 35.0 41.0 31.0 41.0 64-65 36.414963218581335 38.5 35.0 41.0 31.0 41.0 66-67 36.10486701844889 37.5 35.0 40.0 31.0 41.0 68-69 35.79055311518147 37.0 35.0 40.0 30.0 41.0 70-71 35.445118791382754 36.5 35.0 39.0 30.0 41.0 72-73 35.07194080481277 36.0 34.5 39.0 30.0 41.0 74-75 34.71263653327908 35.5 34.0 38.5 29.0 40.0 76-77 34.110445973131604 35.0 34.0 37.0 29.0 39.0 78-79 34.079140117657005 35.0 34.0 37.0 29.0 39.0 80-81 33.83082179911243 35.0 34.0 36.5 29.0 39.0 82-83 33.564932368569316 35.0 34.0 36.0 29.0 37.5 84-85 33.307000315616136 35.0 34.0 36.0 29.0 37.0 86-87 33.08249343443096 35.0 34.0 35.0 29.0 37.0 88-89 32.86983764605618 35.0 34.0 35.0 27.5 36.0 90-91 32.723144792459365 35.0 34.0 35.0 27.0 36.0 92-93 32.610372301461126 35.0 33.0 35.0 27.0 36.0 94-95 32.49111126180316 35.0 33.0 35.0 27.0 36.0 96-97 32.39550971168424 35.0 33.0 35.0 27.0 35.0 98-99 32.29945943411785 35.0 33.0 35.0 26.0 35.0 100-101 31.846098206011106 34.5 32.5 35.0 24.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06740276365317044 1101 2 0.1328242636912904 1101 3 0.11713737098639854 1101 4 0.07190227989124054 1101 5 0.057975930808090936 1101 6 0.014987262428022063 1101 7 0.17644844566061124 1101 8 0.07023248790323322 1101 9 0.11636859786437981 1101 10-11 0.13559735149969754 1101 12-13 0.18344282877842488 1101 14-15 0.19264667939923896 1101 16-17 0.14610638755198835 1101 18-19 0.10523111811357211 1101 20-21 0.08129165342440103 1101 22-23 0.19633223126333377 1101 24-25 0.12240653104267096 1101 26-27 0.19321331380000117 1101 28-29 0.2922555771951565 1101 30-31 0.12790178264374674 1101 32-33 0.14714837404432046 1101 34-35 0.19679373934533828 1101 36-37 0.21962181672232362 1101 38-39 0.08078230201500247 1101 40-41 0.15089571230369359 1101 42-43 0.10567948446620079 1101 44-45 0.003877231040270601 1101 46-47 -0.010589270901370185 1101 48-49 -0.0405136565186055 1101 50-51 0.15919990652484728 1101 52-53 0.027828664360050936 1101 54-55 0.15714344642646694 1101 56-57 0.20788122510389684 1101 58-59 0.11883235375208301 1101 60-61 -0.1411272907315606 1101 62-63 -0.1355877018344529 1101 64-65 -0.04074853770240594 1101 66-67 -0.11782037241739829 1101 68-69 -0.151034948261632 1101 70-71 -0.14857697030975459 1101 72-73 -0.0701842733643403 1101 74-75 -0.15476290124252756 1101 76-77 -0.2591776345511647 1101 78-79 0.005961369606424682 1101 80-81 0.0734195989536559 1101 82-83 0.1531496570051587 1101 84-85 4.980101293412531E-4 1101 86-87 0.09603131155849098 1101 88-89 0.007787808214622771 1101 90-91 -0.1174494268991495 1101 92-93 -0.321997922998456 1101 94-95 -0.14583622915888128 1101 96-97 -0.016064131253756386 1101 98-99 -0.39035558371053014 1101 100-101 -0.24471084038366087 1102 1 0.061387937942207316 1102 2 0.17462325519119304 1102 3 0.20514929071411103 1102 4 0.20879227823239432 1102 5 0.19749410519506228 1102 6 0.10630488799587567 1102 7 0.2430802032208632 1102 8 0.17005621023993456 1102 9 0.26491750492288446 1102 10-11 0.2583421665980197 1102 12-13 0.1812468527857618 1102 14-15 0.27675181043829156 1102 16-17 0.2610755989192981 1102 18-19 0.39005095314805516 1102 20-21 0.25781014958806736 1102 22-23 0.19351218837309858 1102 24-25 0.24678021570551323 1102 26-27 0.3110750177774477 1102 28-29 -0.10256391807512699 1102 30-31 -0.15305273492106153 1102 32-33 0.1575396624901373 1102 34-35 0.16125490329755365 1102 36-37 0.15305651304307588 1102 38-39 0.016432960458040213 1102 40-41 0.1343576896913916 1102 42-43 0.1396019422804784 1102 44-45 0.24639742202771941 1102 46-47 0.36129214339710813 1102 48-49 0.3344940881066307 1102 50-51 0.34477211940910024 1102 52-53 0.08807735177098408 1102 54-55 -0.03429269689006986 1102 56-57 -0.09586297642024988 1102 58-59 -0.03229926090735091 1102 60-61 0.2731437051972634 1102 62-63 0.11645887783925701 1102 64-65 0.2584807971392138 1102 66-67 0.27889780516735385 1102 68-69 0.3233363380656584 1102 70-71 0.41011529725656004 1102 72-73 0.4539204979243934 1102 74-75 0.3646461288905627 1102 76-77 0.32868988538999133 1102 78-79 0.149916464770385 1102 80-81 0.08700567022020067 1102 82-83 -0.32200458595023207 1102 84-85 -0.13473014153340301 1102 86-87 -0.05322510448876727 1102 88-89 0.012442577804520738 1102 90-91 0.15261265001946356 1102 92-93 0.24626969927168574 1102 94-95 0.27451104982949914 1102 96-97 0.23451996338457093 1102 98-99 0.0945668800995847 1102 100-101 0.22506063821019495 1103 1 0.05691719185950461 1103 2 0.0751283105364422 1103 3 0.10085967453700562 1103 4 0.04773137449958398 1103 5 0.09644949357016941 1103 6 -0.0014482386513066103 1103 7 0.12688488959778255 1103 8 0.1265687407876328 1103 9 0.17458064163283638 1103 10-11 0.2004218062549299 1103 12-13 0.12277687339049237 1103 14-15 0.1602982189868598 1103 16-17 0.12839554716789792 1103 18-19 0.11980273809223263 1103 20-21 0.12005750212053101 1103 22-23 0.14984933917093457 1103 24-25 -0.001559426826460708 1103 26-27 0.1576814675354612 1103 28-29 0.17282972800278884 1103 30-31 0.11188808639284531 1103 32-33 0.16548398181544854 1103 34-35 0.04312130801267955 1103 36-37 0.22233774357640357 1103 38-39 0.22760263797014346 1103 40-41 0.17440512847618805 1103 42-43 0.21498648394514674 1103 44-45 0.24667007524541162 1103 46-47 0.19135252003230363 1103 48-49 0.180373622344014 1103 50-51 0.3809511409242674 1103 52-53 0.33797480732707186 1103 54-55 0.31250111210892584 1103 56-57 0.3775847211215577 1103 58-59 0.44954222646627784 1103 60-61 0.2931882689042027 1103 62-63 0.25299776326740187 1103 64-65 0.20972810175371137 1103 66-67 0.17216101854605625 1103 68-69 0.1624206257651153 1103 70-71 0.2699581263331723 1103 72-73 0.30576342283693947 1103 74-75 0.262027412133655 1103 76-77 0.25686037750749335 1103 78-79 0.3589911688769547 1103 80-81 0.46405056935288513 1103 82-83 0.26793168315705174 1103 84-85 0.2513324393281664 1103 86-87 0.17082971366255606 1103 88-89 0.2804573365375731 1103 90-91 0.39241187374844344 1103 92-93 0.20082717687003537 1103 94-95 0.24479424499936897 1103 96-97 0.23458321509763636 1103 98-99 0.33284566681672345 1103 100-101 0.4073602213239056 1104 1 0.0533879974766478 1104 2 0.04349120780177884 1104 3 0.07594467084151546 1104 4 0.08117834419655168 1104 5 0.061037446267874884 1104 6 0.19302238219126622 1104 7 0.08496231014471789 1104 8 0.19889320494136342 1104 9 0.13171663572004633 1104 10-11 0.0548115882209288 1104 12-13 0.14568060583690112 1104 14-15 0.1542048154391864 1104 16-17 0.309453843546315 1104 18-19 0.26137563166207656 1104 20-21 0.09967086132230918 1104 22-23 0.15479898809923753 1104 24-25 0.22806686659555453 1104 26-27 0.25328568039576993 1104 28-29 0.13845103251129132 1104 30-31 0.17695876266779464 1104 32-33 0.18499248144590297 1104 34-35 0.2348069140732889 1104 36-37 0.12194232598586296 1104 38-39 0.22017470005440032 1104 40-41 0.14116880179474123 1104 42-43 0.25448666871972847 1104 44-45 0.0611457773887949 1104 46-47 0.3938072502614247 1104 48-49 0.3688566230831114 1104 50-51 0.34127819192205777 1104 52-53 0.40504243482153157 1104 54-55 0.3242190537201637 1104 56-57 0.26280691254802235 1104 58-59 0.3140516499695991 1104 60-61 0.3726750575220805 1104 62-63 0.35667662505602493 1104 64-65 0.07856771742260094 1104 66-67 0.31415242652831665 1104 68-69 0.17125746981538015 1104 70-71 0.24728767178772415 1104 72-73 0.23710257656937728 1104 74-75 0.07372364273232535 1104 76-77 0.1317527463175452 1104 78-79 0.28370430634923594 1104 80-81 0.1954876536100869 1104 82-83 0.24489445108019225 1104 84-85 0.19956876600961238 1104 86-87 0.18979913347039457 1104 88-89 0.201251405273716 1104 90-91 0.04542647093987284 1104 92-93 0.1387904991169009 1104 94-95 0.07314882740882922 1104 96-97 0.14922013305772452 1104 98-99 0.13054614723061775 1104 100-101 0.08681236470897602 1105 1 0.07158328052226182 1105 2 0.12075472227296302 1105 3 0.03934150163777872 1105 4 0.1278810470907885 1105 5 0.07386627241522348 1105 6 0.05835691318880265 1105 7 0.0924574557069846 1105 8 0.06764976020400582 1105 9 0.09595324881072997 1105 10-11 0.13161667723076675 1105 12-13 0.10847949037923854 1105 14-15 0.0864389020962193 1105 16-17 0.10273673679120776 1105 18-19 -0.050644682565319954 1105 20-21 0.028420914473088033 1105 22-23 -0.005134803748141792 1105 24-25 -0.005652607140874011 1105 26-27 0.02761279154467644 1105 28-29 0.00597581849081763 1105 30-31 -0.09281199802614992 1105 32-33 0.10672973234592575 1105 34-35 -0.044268776178157054 1105 36-37 -0.012227954933713647 1105 38-39 -0.07064509754744108 1105 40-41 -0.16766290866817002 1105 42-43 -0.12603658297401665 1105 44-45 0.013570072963823065 1105 46-47 0.08318258688302649 1105 48-49 0.012811962255945275 1105 50-51 0.04372419073148137 1105 52-53 -0.14827311071805838 1105 54-55 -0.20024934991393906 1105 56-57 -0.07602088247423211 1105 58-59 -0.11859302644846537 1105 60-61 -0.11749780652530717 1105 62-63 -0.025787515885667744 1105 64-65 -0.1776473802330969 1105 66-67 -0.1057294192919116 1105 68-69 -0.1283366354089921 1105 70-71 -0.1529230710475531 1105 72-73 -0.1467625302991209 1105 74-75 -0.2639645110222517 1105 76-77 -0.21272231888074344 1105 78-79 0.0036355823931160103 1105 80-81 -0.059924424372731266 1105 82-83 0.07488415754615829 1105 84-85 -0.004578943546185599 1105 86-87 0.11889378083269975 1105 88-89 0.1777468024163369 1105 90-91 -0.053387026523068926 1105 92-93 0.02429885087583017 1105 94-95 -0.056899610666974354 1105 96-97 0.18932505269374644 1105 98-99 0.09537184811221522 1105 100-101 0.1748415692898675 1106 1 0.10141588485352315 1106 2 0.07731371095554351 1106 3 0.1525678557463621 1106 4 0.15657105563332863 1106 5 0.14253387646891014 1106 6 -0.17189478102638134 1106 7 0.068194487990489 1106 8 0.12491046344312196 1106 9 0.25225438701510683 1106 10-11 0.22007960832453222 1106 12-13 0.14247399365664393 1106 14-15 0.06847553973261 1106 16-17 0.1779840023084276 1106 18-19 0.15773529226277105 1106 20-21 0.07670076509877788 1106 22-23 0.10269342739923815 1106 24-25 0.10660017912743314 1106 26-27 0.20284381712175303 1106 28-29 0.27690110565190906 1106 30-31 0.11382591179413737 1106 32-33 0.06736693456880971 1106 34-35 -0.0868344050076928 1106 36-37 0.06789543546864962 1106 38-39 0.07714788630128311 1106 40-41 0.052983192379059574 1106 42-43 0.20335953153745123 1106 44-45 0.08133619462582686 1106 46-47 0.0032153816192916906 1106 48-49 0.1628433235689286 1106 50-51 0.11584236778162449 1106 52-53 -0.025603148558779765 1106 54-55 0.0711059642336167 1106 56-57 0.00549243137669464 1106 58-59 0.0823426758170882 1106 60-61 0.0737979169847307 1106 62-63 0.11034875378351217 1106 64-65 0.10938071900619661 1106 66-67 0.06138826476005477 1106 68-69 -0.016684561212350957 1106 70-71 -0.07987358314424142 1106 72-73 0.021480763889890397 1106 74-75 0.1451162669371655 1106 76-77 0.12181879095345494 1106 78-79 0.09151768705621777 1106 80-81 0.03117666842609168 1106 82-83 0.0696819910055737 1106 84-85 -0.006643321651765177 1106 86-87 0.11776527070635012 1106 88-89 0.17926266825785575 1106 90-91 0.1562387008563988 1106 92-93 0.045305130595544085 1106 94-95 0.08622439039028507 1106 96-97 -0.04965105222289168 1106 98-99 0.1580451742590938 1106 100-101 0.32409076205712495 1107 1 0.12227200728755605 1107 2 0.06152071963024497 1107 3 0.06931835546846088 1107 4 0.02969830226285808 1107 5 0.012393368413611938 1107 6 0.0015490654731706854 1107 7 0.15379439733516165 1107 8 0.12352134007863924 1107 9 0.17366022302916662 1107 10-11 0.08287823708555209 1107 12-13 0.04640208301073301 1107 14-15 0.055651973046600745 1107 16-17 0.006367909136947958 1107 18-19 -0.08341405699999882 1107 20-21 -0.03843960034396332 1107 22-23 -0.060414092445107315 1107 24-25 0.04582217753787887 1107 26-27 0.01697841716291748 1107 28-29 -0.02143973218528572 1107 30-31 -0.17196001057375554 1107 32-33 0.11492166888161393 1107 34-35 -0.09162194699167259 1107 36-37 0.04777704392240878 1107 38-39 0.025796189096254807 1107 40-41 0.04482267077171542 1107 42-43 0.0172917759275677 1107 44-45 -0.007163374838434322 1107 46-47 0.16495459182326044 1107 48-49 0.027764566749546304 1107 50-51 -0.05123209370181314 1107 52-53 0.23031045444333387 1107 54-55 0.2467888278320416 1107 56-57 0.0030664762002103885 1107 58-59 0.12333492000678348 1107 60-61 0.26468713324010196 1107 62-63 0.09541683393330658 1107 64-65 -0.09359780200140477 1107 66-67 0.11713204319102744 1107 68-69 0.14268766603343153 1107 70-71 0.1248232264202116 1107 72-73 0.15387185222410693 1107 74-75 0.013206592332757339 1107 76-77 -0.10863538763292979 1107 78-79 0.11311500933206275 1107 80-81 0.0933719304516103 1107 82-83 0.20151804669693263 1107 84-85 0.24554501017450292 1107 86-87 0.0577982334783016 1107 88-89 0.03888566233518986 1107 90-91 0.1568449969308503 1107 92-93 0.23734334150949365 1107 94-95 0.055908044910211174 1107 96-97 0.12659917807711452 1107 98-99 0.24232071404761513 1107 100-101 0.29422345340270084 1108 1 0.11500096257062609 1108 2 0.17570256184857413 1108 3 0.1219937694222537 1108 4 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-1.1318281805359405 2316 64-65 -0.9481311189092594 2316 66-67 -1.0958302227031922 2316 68-69 -1.0456780220627735 2316 70-71 -1.199072017834581 2316 72-73 -1.488214776184897 2316 74-75 -1.0256895587450998 2316 76-77 -0.884156468702912 2316 78-79 -0.9079192936745955 2316 80-81 -1.2480646369892199 2316 82-83 -1.5138398860658455 2316 84-85 -1.6411628456508964 2316 86-87 -1.2006372172583895 2316 88-89 -1.0416872787486824 2316 90-91 -0.900705474331982 2316 92-93 -0.9877615134729609 2316 94-95 -0.9022386482653459 2316 96-97 -0.8240425560879885 2316 98-99 -0.8648729023742092 2316 100-101 -0.9857245211880006 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 18.0 14 55.0 15 191.0 16 464.0 17 970.0 18 1734.0 19 2745.0 20 3814.0 21 5575.0 22 6996.0 23 6954.0 24 6674.0 25 6545.0 26 7323.0 27 8667.0 28 10668.0 29 13215.0 30 16566.0 31 20683.0 32 26563.0 33 35124.0 34 49740.0 35 77737.0 36 139588.0 37 274511.0 38 363706.0 39 107559.0 40 103.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.40029549273078 19.35879417524193 13.156378304401068 23.084532027626224 2 15.80773764475015 19.869197436189378 40.601956345565235 23.721108573495233 3 19.083203221472015 25.28637864853891 29.538336605315262 26.092081524673816 4 12.359269284514033 16.16731874235489 37.88759292392525 33.58581904920583 5 12.745952453308487 38.620447739577344 34.97796965899225 13.655630148121917 6 31.760610604199783 37.12834525893499 17.362403504762288 13.748640632102932 7 28.569036634075324 32.365220608980074 21.44230712882245 17.62343562812215 8 26.11283988383317 36.45408203842815 19.46112521756165 17.97195286017703 9 26.341975522587486 15.98315262844614 20.24589594378852 37.428975905177865 10-11 24.97791942830784 26.507611204456467 28.12629500983266 20.38817435740304 12-13 25.298516771606938 24.057609571959222 28.419809642449618 22.224064013984226 14-15 22.62134686882843 25.70312493459546 25.88228104235368 25.79324715422243 16-17 21.981868397281183 29.061632212603044 26.64013649351313 22.316362896602648 18-19 21.85645912185043 27.877318250733158 28.797083941333913 21.469138686082502 20-21 22.57785546790301 26.978691306491733 28.9078844593797 21.53556876622556 22-23 22.529362665441006 26.72469293630003 28.623000035999564 22.1229443622594 24-25 22.159894314639985 27.00372293089346 28.729105081754625 22.10727767271193 26-27 21.907192113112803 27.11653267631598 28.839487010763598 22.136788199807615 28-29 21.99764034820355 26.837187375444216 29.06918519163588 22.095987084716356 30-31 22.241782956511255 27.063157401158517 28.694759881636394 22.000299760693835 32-33 21.890332137982075 27.27686369521348 28.73815863442804 22.09464553237641 34-35 22.104933574105747 27.195436709756223 28.835719709432233 21.863910006705797 36-37 22.37559324531243 26.910084563357216 28.47150538849667 22.24281680283368 38-39 22.106691648060384 27.326496937184018 28.550241986322185 22.016569428433414 40-41 22.09488743722211 27.11674197083439 28.912865668917835 21.875504923025662 42-43 22.138336979243846 26.96023152996804 28.4914720855529 22.40995940523521 44-45 22.1015848618112 27.256508850228006 28.439315891565347 22.202590396395447 46-47 22.335073826548424 27.107533012024387 28.191008874924762 22.36638428650243 48-49 22.035652731740242 27.37242415461688 28.102027177545107 22.489895936097763 50-51 22.130381979403765 27.373634659011703 27.873741621450094 22.62224174013444 52-53 22.269606501190047 27.460110557736407 27.864509426206517 22.40577351486703 54-55 22.330958656853177 27.406479061783028 27.85248960111044 22.41007268025335 56-57 22.369021397434384 27.58928713449852 27.608500371288475 22.433191096778625 58-59 22.26713682587282 27.33110141658902 27.837970881272245 22.56379087626592 60-61 22.360942629023793 27.691422859482174 27.769908303885593 22.17772620760844 62-63 22.158429253011118 27.669698088471307 27.96116163480786 22.21071102370972 64-65 22.171238077537758 27.46090587690636 27.926167591329847 22.441688454226032 66-67 22.273708673750868 27.52465698721378 27.615407090395976 22.586227248639375 68-69 22.194511627984852 27.482379494495135 27.639434101109345 22.68367477641067 70-71 22.171363654248804 27.11812331465589 27.975854110000174 22.73465892109513 72-73 22.14256472851571 27.45960825089222 27.62331842319184 22.774508597400228 74-75 21.98718447804877 27.65056856948871 27.509211051755184 22.853035900707333 76-77 21.976384880898863 27.463919717971454 27.845338048320244 22.714357352809444 78-79 22.048470200374805 27.390723963812064 27.502822342487754 23.057983493325374 80-81 22.133787692938867 27.577897693734275 27.3393170372293 22.94899757609756 82-83 22.2710182020028 27.384340132397988 27.518002325797514 22.826639339801698 84-85 22.14369491891512 27.345459020551882 27.377606658579527 23.13323940195347 86-87 21.97362219325586 27.52834057073778 27.578445678444925 22.919591557561432 88-89 22.093254939978518 27.277354584261555 27.471538038441544 23.157852437318386 90-91 22.048005465098463 27.247006879092233 27.679367495221808 23.025620160587497 92-93 22.225026768768906 27.36919301893948 27.46157561936527 22.944204592926347 94-95 22.01895538594328 27.087315161546066 27.627253160137933 23.266476292372722 96-97 22.183084147279715 26.990914106366827 27.543242340448508 23.28275940590495 98-99 21.996853884799275 27.02431751150492 27.559190582751285 23.41963802094452 100-101 21.98617986436041 27.094221880653567 27.49192332453459 23.42767493045143 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 110.0 1 87.0 2 97.0 3 161.0 4 152.5 5 137.0 6 268.5 7 595.5 8 920.5 9 1044.0 10 915.0 11 783.0 12 813.0 13 887.5 14 914.0 15 936.5 16 1006.0 17 1060.0 18 1144.5 19 1244.0 20 1390.5 21 1496.0 22 1673.0 23 2111.0 24 2472.5 25 2880.5 26 3738.0 27 4973.0 28 6486.0 29 8957.0 30 12177.0 31 15651.5 32 20691.5 33 25818.0 34 29999.0 35 34204.5 36 40501.5 37 45279.0 38 44290.5 39 42691.0 40 40785.0 41 37984.0 42 36327.0 43 36861.5 44 38212.5 45 38930.0 46 38970.5 47 39063.0 48 40199.0 49 40269.5 50 40133.0 51 39910.0 52 39427.5 53 39281.5 54 38665.0 55 37099.5 56 34759.0 57 31781.5 58 28830.0 59 25555.0 60 21631.0 61 18010.0 62 14229.5 63 10446.5 64 7356.5 65 5206.5 66 3676.0 67 2709.0 68 2026.0 69 1438.5 70 1092.5 71 845.5 72 625.5 73 471.5 74 346.0 75 229.0 76 140.5 77 88.5 78 63.0 79 34.5 80 19.0 81 15.0 82 12.5 83 8.0 84 6.5 85 5.5 86 2.5 87 1.5 88 1.5 89 1.5 90 1.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04612851185737164 2 0.0014232027251820654 3 3.3487122945460363E-4 4 0.008204345121637788 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.002511534220909527 24-25 0.0 26-27 0.0 28-29 0.02051086280409447 30-31 0.016994714894821135 32-33 4.1858903681825454E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.034742890055915125 50-51 0.012097223164047556 52-53 0.0 54-55 8.790369773183345E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.0929451840912724E-4 80-81 0.01121818618672922 82-83 0.005023068441819054 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1194489.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.547495385840215 #Duplication Level Percentage of deduplicated Percentage of total 1 73.87642727994354 40.29774076175081 2 14.584199328172415 15.910630911193174 3 4.984736056938067 8.157146011963818 4 2.2649074508762483 4.941801149041091 5 1.2630453574730238 3.4447980404433336 6 0.7034701253555011 2.3023520050143387 7 0.46885941821406946 1.790257486614778 8 0.3293622704166903 1.4372709540659416 9 0.23837100875980863 1.1702287350399365 >10 1.1481325439311458 11.213150480154676 >50 0.0877513050736783 3.3278701192224833 >100 0.048720756296856814 4.724169215818828 >500 0.0015516142683684477 0.558686672825394 >1k 4.654842805105343E-4 0.7238974568513248 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3437 0.28773810390886817 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3262 0.2730874876202293 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1854 0.15521281485220878 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.37178073636509E-5 0.0 5 0.0 0.0 0.0 8.37178073636509E-5 0.0 6 0.0 0.0 0.0 8.37178073636509E-5 0.0 7 0.0 0.0 0.0 8.37178073636509E-5 0.0 8 0.0 0.0 0.0 8.37178073636509E-5 0.0 9 0.0 0.0 0.0 8.37178073636509E-5 0.0 10-11 0.0 0.0 0.0 2.0929451840912724E-4 0.0 12-13 0.0 0.0 0.0 7.534602662728581E-4 0.0 14-15 0.0 0.0 0.0 0.0010464725920456362 0.0 16-17 0.0 0.0 0.0 0.0020929451840912727 0.0 18-19 0.0 0.0 0.0 0.0039347369460915925 0.0 20-21 0.0 0.0 0.0 0.006781142396455723 0.0 22-23 0.0 0.0 0.0 0.012892542334002239 0.0 24-25 0.0 0.0 0.0 0.01988297924886709 0.0 26-27 0.0 0.0 0.0 0.022938679217640347 0.0 28-29 0.0 0.0 0.0 0.025450213438549873 0.0 30-31 0.0 0.0 0.0 0.03139417776136909 0.0 32-33 0.0 0.0 0.0 0.04395184886591672 0.0 34-35 0.0 0.0 0.0 0.06161630621964706 0.0 36-37 0.0 0.0 0.0 0.08882459361283361 0.0 38-39 0.0 0.0 0.0 0.12783709184429493 0.0 40-41 0.0 4.185890368182545E-5 0.0 0.1773143159962126 0.0 42-43 0.0 1.674356147273018E-4 0.0 0.2251590429045391 0.0 44-45 0.0 1.674356147273018E-4 0.0 0.2789477341356848 0.0 46-47 0.0 1.674356147273018E-4 0.0 0.34780563069228765 0.0 48-49 0.0 1.674356147273018E-4 0.0 0.4129380848212081 0.0 50-51 0.0 1.674356147273018E-4 0.0 0.4902933388252215 0.0 52-53 0.0 1.674356147273018E-4 0.0 0.5659323777782801 0.0 54-55 0.0 2.511534220909527E-4 0.0 0.6434969263007027 0.0 56-57 0.0 2.511534220909527E-4 0.0 0.7398561225762648 0.0 58-59 0.0 2.511534220909527E-4 0.0 0.8470567749054199 0.0 60-61 0.0 2.511534220909527E-4 0.0 0.9629640792003945 0.0 62-63 0.0 2.511534220909527E-4 0.0 1.0757319657192324 0.0 64-65 0.0 2.511534220909527E-4 0.0 1.1889184412748883 0.0 66-67 0.0 2.511534220909527E-4 0.0 1.3257551974107757 0.0 68-69 0.0 2.511534220909527E-4 0.0 1.4799215396709386 0.0 70-71 0.0 2.511534220909527E-4 0.0 1.648905933834468 0.0 72-73 0.0 2.511534220909527E-4 0.0 1.8242947402613168 0.0 74-75 0.0 2.511534220909527E-4 0.0 1.9926512508696188 0.0 76-77 0.0 2.511534220909527E-4 0.0 2.174821199692923 0.0 78-79 0.0 2.511534220909527E-4 0.0 2.403119660373599 0.0 80-81 0.0 2.511534220909527E-4 0.0 2.6549846838271427 0.0 82-83 0.0 2.511534220909527E-4 0.0 2.919909685229416 0.0 84-85 0.0 2.511534220909527E-4 0.0 3.178723286694143 0.0 86-87 0.0 2.511534220909527E-4 0.0 3.4528991058100997 0.0 88-89 0.0 2.511534220909527E-4 0.0 3.7588876917242438 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2480 0.0 41.20349 1 GTATCAA 4100 0.0 37.442593 1 TCAACGC 4865 0.0 31.346394 4 CAACGCA 4910 0.0 30.95457 5 ATCAACG 4955 0.0 30.777033 3 AACGCAG 5110 0.0 29.835987 6 GTACATG 4775 0.0 29.064093 1 TATCAAC 5415 0.0 28.163729 2 CGTATAC 85 6.401137E-4 27.945795 3 ACATGGG 4920 0.0 26.650745 3 TACATGG 5180 0.0 26.2313 2 ACGCAGA 5805 0.0 26.182074 7 CGCAGAG 5990 0.0 25.373447 8 GGACCGT 225 1.0186341E-10 25.331156 6 GTGTAGC 620 0.0 24.53045 1 TCGCACG 50 0.0016529782 23.752932 22-23 TACGCTG 100 0.0016545107 23.74796 5 GCAGAGT 6445 0.0 23.28737 9 CATGGGG 2800 0.0 22.905571 4 CCGATCG 55 0.0028797009 21.589054 18-19 >>END_MODULE