##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031607_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1067003 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.030670954064796 33.0 31.0 34.0 30.0 34.0 2 32.24017177083851 34.0 31.0 34.0 30.0 34.0 3 32.37197833558106 34.0 31.0 34.0 30.0 34.0 4 35.48582899954358 37.0 35.0 37.0 33.0 37.0 5 35.29020255800593 37.0 35.0 37.0 33.0 37.0 6 35.529949775211506 37.0 35.0 37.0 33.0 37.0 7 35.65035243574761 37.0 35.0 37.0 33.0 37.0 8 35.70953502473751 37.0 36.0 37.0 35.0 37.0 9 37.61244720024217 39.0 38.0 39.0 35.0 39.0 10-11 37.56013853756737 39.0 38.0 39.0 35.0 39.0 12-13 37.62139844030429 39.0 38.0 39.0 35.0 39.0 14-15 38.96315521137241 41.0 39.0 41.0 36.0 41.0 16-17 38.93941957051667 41.0 39.0 41.0 36.0 41.0 18-19 38.957121020278294 41.0 39.0 41.0 36.0 41.0 20-21 38.991369752474924 41.0 39.0 41.0 35.5 41.0 22-23 38.90896229907507 41.0 39.0 41.0 35.0 41.0 24-25 38.82274229781922 41.0 39.0 41.0 35.0 41.0 26-27 38.7026001801307 40.5 38.5 41.0 34.5 41.0 28-29 38.62769083123477 40.0 38.0 41.0 34.0 41.0 30-31 38.47995272740564 40.0 38.0 41.0 34.0 41.0 32-33 38.29019974639247 40.0 38.0 41.0 34.0 41.0 34-35 38.15132853422155 40.0 38.0 41.0 34.0 41.0 36-37 37.993459249880274 40.0 38.0 41.0 33.5 41.0 38-39 37.797681918420096 40.0 38.0 41.0 33.0 41.0 40-41 37.729041530342464 40.0 38.0 41.0 33.0 41.0 42-43 37.546656382409424 40.0 37.0 41.0 32.5 41.0 44-45 37.273785546994716 40.0 37.0 41.0 31.0 41.0 46-47 37.02113349259562 40.0 37.0 41.0 30.5 41.0 48-49 37.05973179081971 40.0 37.0 41.0 30.5 41.0 50-51 36.78786376420685 39.5 37.0 40.5 30.0 41.0 52-53 36.98641990697308 39.5 37.5 40.5 31.0 41.0 54-55 37.30901506368773 40.0 38.0 41.0 31.0 41.0 56-57 37.205248720012975 40.0 38.0 41.0 31.0 41.0 58-59 37.0624243793129 40.0 37.0 41.0 30.5 41.0 60-61 36.77246408866704 40.0 37.0 41.0 29.0 41.0 62-63 36.50899575727529 39.5 36.0 41.0 28.0 41.0 64-65 36.289977160326636 39.0 36.0 41.0 28.0 41.0 66-67 36.05128757838544 39.0 35.5 41.0 28.0 41.0 68-69 35.752794509481234 38.5 35.0 41.0 28.0 41.0 70-71 35.3597290729267 37.5 35.0 40.0 27.5 41.0 72-73 34.966067574317975 37.0 35.0 39.5 26.5 41.0 74-75 34.51117944373165 36.5 34.0 39.0 26.0 41.0 76-77 34.09762109384884 36.0 34.0 39.0 26.0 40.5 78-79 33.70655518306884 36.0 34.0 37.5 26.0 39.5 80-81 33.289912961819226 35.0 34.0 37.0 25.5 39.0 82-83 32.9054140428846 35.0 33.5 37.0 24.5 39.0 84-85 32.56744732676478 35.0 33.0 36.0 24.0 37.5 86-87 32.368802618174456 35.0 33.0 36.0 24.0 37.0 88-89 32.08230061208825 35.0 33.0 35.5 23.5 37.0 90-91 31.851846714582805 35.0 33.0 35.0 22.0 36.0 92-93 31.652087201254353 35.0 33.0 35.0 20.0 36.0 94-95 31.450193204705144 35.0 32.0 35.0 20.0 36.0 96-97 31.32319262457556 35.0 32.0 35.0 20.0 36.0 98-99 31.23110853484011 35.0 32.0 35.0 19.0 35.0 100-101 30.766480038013015 34.5 31.5 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21520670167136302 1101 2 0.24078924825444403 1101 3 0.4596488489817858 1101 4 0.26827095247866595 1101 5 0.13793677435096896 1101 6 0.22268314714601445 1101 7 0.3646474699366067 1101 8 0.29657149299002583 1101 9 0.40094492392933034 1101 10-11 0.5187301563036542 1101 12-13 0.4796456462783141 1101 14-15 0.5870689354707253 1101 16-17 0.5307763791754141 1101 18-19 0.5307327195860054 1101 20-21 0.2892238805196641 1101 22-23 0.432306586260637 1101 24-25 0.5556672214799292 1101 26-27 0.483761635462173 1101 28-29 0.5785421376213691 1101 30-31 0.5509280963755856 1101 32-33 0.7387229303678566 1101 34-35 0.7077511628774289 1101 36-37 0.809998702716392 1101 38-39 0.6750001772390348 1101 40-41 0.6226721383339893 1101 42-43 0.5494673440697326 1101 44-45 0.7255081730607458 1101 46-47 0.945352895378349 1101 48-49 1.0122168218273515 1101 50-51 0.928118780913401 1101 52-53 1.0376864896808797 1101 54-55 0.986216750467868 1101 56-57 1.1455885713772176 1101 58-59 1.0113656517289158 1101 60-61 1.0109811057390559 1101 62-63 0.7834319462887294 1101 64-65 0.9421699473065601 1101 66-67 0.9250439687158689 1101 68-69 0.8069319266947517 1101 70-71 0.8599499552783456 1101 72-73 0.7773592827710161 1101 74-75 0.6790398673274396 1101 76-77 0.6718499225574632 1101 78-79 0.7057364511315285 1101 80-81 0.7284027196637766 1101 82-83 0.7524347193218119 1101 84-85 0.5126573642915133 1101 86-87 0.5687697968357668 1101 88-89 0.676942280739631 1101 90-91 0.5594263944663602 1101 92-93 0.3897574007426954 1101 94-95 0.4910453459527524 1101 96-97 0.6827140755556691 1101 98-99 0.349758931524228 1101 100-101 0.36061215249899803 1102 1 -0.1023331854862839 1102 2 0.09001541871365504 1102 3 0.08585816781749145 1102 4 0.09504672500043654 1102 5 0.06735275910604344 1102 6 -0.16872289851403366 1102 7 -0.167307841500822 1102 8 -0.002740243362403305 1102 9 0.2602118222591159 1102 10-11 0.06104678747355052 1102 12-13 0.19994531372964985 1102 14-15 0.2690724477824986 1102 16-17 0.20330412270192255 1102 18-19 0.21569479409185277 1102 20-21 0.21527701357648965 1102 22-23 0.18937193767863647 1102 24-25 0.35129028316958255 1102 26-27 0.21551143929582395 1102 28-29 0.027522446256426747 1102 30-31 0.2471325577588388 1102 32-33 0.38414384730541684 1102 34-35 0.12289371042441388 1102 36-37 0.20776502188838464 1102 38-39 0.08630533734813639 1102 40-41 0.26221404320265407 1102 42-43 0.4264856155639549 1102 44-45 -0.07020904847644971 1102 46-47 -0.5903217300373313 1102 48-49 0.28087579130013296 1102 50-51 0.16174879249655305 1102 52-53 0.37458070931246823 1102 54-55 0.32045132805632903 1102 56-57 0.2928000200190013 1102 58-59 0.26303399982143816 1102 60-61 0.10663704864531098 1102 62-63 -0.25118450179257934 1102 64-65 0.012633647190725128 1102 66-67 0.0590655656967769 1102 68-69 0.350293283792233 1102 70-71 0.36978667014589206 1102 72-73 0.3474254188544137 1102 74-75 0.17006852629690883 1102 76-77 0.3659701631881802 1102 78-79 0.3622674827796928 1102 80-81 0.3429040834869568 1102 82-83 0.15504205033851548 1102 84-85 0.19523480329312548 1102 86-87 0.1786648011327472 1102 88-89 -0.13002405337837075 1102 90-91 -0.1632168069684532 1102 92-93 -0.13880292508026315 1102 94-95 -0.12448280858896865 1102 96-97 -0.2507986098682373 1102 98-99 0.055429446787840675 1102 100-101 -0.047772557735278554 1103 1 0.17673793812092242 1103 2 0.15634469081612679 1103 3 0.023436008075748305 1103 4 0.16596206395701074 1103 5 -0.07237024412211213 1103 6 0.146720192186784 1103 7 0.18730217620197465 1103 8 0.27695151962833364 1103 9 0.4629234748676794 1103 10-11 0.4605969280546205 1103 12-13 0.38631623948201366 1103 14-15 0.40530487514894986 1103 16-17 0.41056643656217773 1103 18-19 0.45110664455524585 1103 20-21 0.14298085612569622 1103 22-23 0.23388511064541717 1103 24-25 0.48400297711938833 1103 26-27 0.23707553384961244 1103 28-29 0.30807435711761855 1103 30-31 0.4246687443671888 1103 32-33 0.335783959770275 1103 34-35 0.39205334803765624 1103 36-37 0.3476150687487447 1103 38-39 0.22141311616726256 1103 40-41 0.06879948525200774 1103 42-43 0.05817987777368927 1103 44-45 0.021864261947371233 1103 46-47 0.23125069524643038 1103 48-49 0.3215693245709872 1103 50-51 0.4111146156768086 1103 52-53 0.209601015466788 1103 54-55 -0.021548047138921333 1103 56-57 0.1710234918507183 1103 58-59 0.08809908364940355 1103 60-61 0.3729350805103522 1103 62-63 0.3485905684979542 1103 64-65 0.38393219972638803 1103 66-67 0.22329341116173396 1103 68-69 0.19998817080671216 1103 70-71 0.37894422507663705 1103 72-73 0.3268505984857768 1103 74-75 0.20392253025401175 1103 76-77 -0.49250531322544333 1103 78-79 -0.2024976947689865 1103 80-81 0.10548869246332515 1103 82-83 0.08424334160166325 1103 84-85 0.06648128876641124 1103 86-87 0.11288119184398226 1103 88-89 0.08985931640190614 1103 90-91 0.0799467877415303 1103 92-93 -0.08494892112317132 1103 94-95 -0.1554335531479616 1103 96-97 0.015951364098231835 1103 98-99 0.00579287488322322 1103 100-101 -0.08030405830801968 1104 1 0.03183143367833452 1104 2 0.15110878480702183 1104 3 0.09807766003698504 1104 4 0.01038251533622514 1104 5 -0.09998548823220688 1104 6 0.022227142436001657 1104 7 0.12394113474815072 1104 8 0.2052122545900943 1104 9 0.20378676083405622 1104 10-11 0.2546667710935324 1104 12-13 0.15488184116617276 1104 14-15 0.2439844051944604 1104 16-17 0.20233565423345112 1104 18-19 0.2531599865570442 1104 20-21 0.24751902831850714 1104 22-23 0.22896653227323327 1104 24-25 0.2740114133907099 1104 26-27 0.33574894953333967 1104 28-29 0.19622023495421814 1104 30-31 0.33530618593246686 1104 32-33 0.14142271708428922 1104 34-35 0.0629981330288345 1104 36-37 0.10123144285480379 1104 38-39 0.1798393258821278 1104 40-41 0.12399127497987905 1104 42-43 0.12263221671056357 1104 44-45 -0.01752886829626732 1104 46-47 0.10484207012646607 1104 48-49 0.19638357180791388 1104 50-51 0.14050186374962692 1104 52-53 -0.07446720223545356 1104 54-55 0.08597958358458158 1104 56-57 0.06306824278722445 1104 58-59 -0.06865109436365202 1104 60-61 0.19852484553310745 1104 62-63 0.20283269722462194 1104 64-65 0.24394814350522154 1104 66-67 0.03469824382945319 1104 68-69 -0.00303185953291063 1104 70-71 0.15880591666513766 1104 72-73 0.10469609862509088 1104 74-75 0.1560021872305768 1104 76-77 -0.0074061676881527205 1104 78-79 0.16323595124816848 1104 80-81 0.154134632217513 1104 82-83 0.19621936401583184 1104 84-85 0.29975773531605654 1104 86-87 0.19281099277893787 1104 88-89 0.33503941918510094 1104 90-91 0.2652332339815864 1104 92-93 0.10192803315069199 1104 94-95 0.0054496238434644795 1104 96-97 0.31355437948475284 1104 98-99 0.33988276374115145 1104 100-101 0.22542564046291957 1105 1 0.2208803146286229 1105 2 0.2720923089595644 1105 3 0.18335578756863669 1105 4 0.24196553389107578 1105 5 -0.0016672349843744882 1105 6 0.2472986386342626 1105 7 0.249907431489099 1105 8 0.2993261409575041 1105 9 0.28627702466234695 1105 10-11 0.19943412783272407 1105 12-13 0.21797479598448177 1105 14-15 0.34516555655167025 1105 16-17 0.2552434509792789 1105 18-19 0.21193498582500325 1105 20-21 0.21224736716656167 1105 22-23 0.21251386617267798 1105 24-25 0.29143342641131653 1105 26-27 0.17341353719792352 1105 28-29 0.09237749730866085 1105 30-31 0.02812142804348383 1105 32-33 0.06405126383256032 1105 34-35 0.09501098282337495 1105 36-37 0.3492811583340938 1105 38-39 0.36147261138864906 1105 40-41 0.2824517397783737 1105 42-43 0.31417004381176383 1105 44-45 0.3222214318413563 1105 46-47 0.28234907333769144 1105 48-49 0.2079364301917579 1105 50-51 0.24118278573336482 1105 52-53 0.2099528489099569 1105 54-55 0.30577060971364034 1105 56-57 0.35316772742896774 1105 58-59 0.325028812520479 1105 60-61 0.5151297765746605 1105 62-63 0.397767203778848 1105 64-65 0.3165582671716791 1105 66-67 0.35791779328139484 1105 68-69 0.36401205328565567 1105 70-71 0.4394974288707303 1105 72-73 0.34175418867614127 1105 74-75 0.1766445793518372 1105 76-77 0.2213554351931677 1105 78-79 0.3364025495908223 1105 80-81 0.35701485534421096 1105 82-83 0.35125203753441525 1105 84-85 0.29869322280788424 1105 86-87 0.3502508676483913 1105 88-89 0.4342794365589242 1105 90-91 0.40347284141133244 1105 92-93 0.3101277686391626 1105 94-95 0.235914446737862 1105 96-97 0.21285088617562664 1105 98-99 0.43039316851212206 1105 100-101 0.3315340511487932 1106 1 0.31116825224873423 1106 2 0.2688330001589776 1106 3 0.3367647308408479 1106 4 0.3065043671150107 1106 5 0.14744755482127658 1106 6 0.12014492466035875 1106 7 0.21957495939657434 1106 8 0.33739057472462264 1106 9 0.3189017454745837 1106 10-11 0.2256317402336876 1106 12-13 0.29683538229855344 1106 14-15 0.22572742297762005 1106 16-17 0.31854916962579694 1106 18-19 0.4169884396190753 1106 20-21 0.23612448727067914 1106 22-23 0.28361594184964645 1106 24-25 0.26828273111093637 1106 26-27 0.18111386606614133 1106 28-29 0.29152408938215757 1106 30-31 0.24723114530268475 1106 32-33 0.3548330121551686 1106 34-35 0.36259289202140366 1106 36-37 0.37779756841727874 1106 38-39 0.3166924198520178 1106 40-41 0.22211103185876624 1106 42-43 0.2778250790202108 1106 44-45 0.4703262313289045 1106 46-47 0.557905274102076 1106 48-49 0.5576704321823627 1106 50-51 0.3832971225120403 1106 52-53 0.44590816531145094 1106 54-55 0.41367406638855186 1106 56-57 0.4235207834404946 1106 58-59 0.40178016849461073 1106 60-61 0.38351131792833826 1106 62-63 0.6157968866486669 1106 64-65 0.6807829188436472 1106 66-67 0.4528286396058334 1106 68-69 0.4752934417850909 1106 70-71 0.349154698710997 1106 72-73 0.37732019561050123 1106 74-75 0.3883798986228797 1106 76-77 0.3754162402254977 1106 78-79 0.21768632184816994 1106 80-81 0.5847721910485006 1106 82-83 0.7009193846830044 1106 84-85 0.5026547799101806 1106 86-87 0.47590371793370423 1106 88-89 0.43900176265474755 1106 90-91 0.4863044746696126 1106 92-93 0.6431302755390647 1106 94-95 0.5606127770905651 1106 96-97 0.5918531422725657 1106 98-99 0.5238517301006311 1106 100-101 0.48695111275481295 1107 1 0.05722933906128702 1107 2 0.04205868286061332 1107 3 0.06807757021636007 1107 4 0.1350479334961605 1107 5 0.054721561788902306 1107 6 0.020666509020934143 1107 7 0.08465022369752262 1107 8 0.0993788538912952 1107 9 0.10368503897261405 1107 10-11 0.1566702202056618 1107 12-13 0.08792561524933262 1107 14-15 0.04187283450112744 1107 16-17 0.12587640585247328 1107 18-19 0.21375455420545109 1107 20-21 -0.007043298215400284 1107 22-23 0.21026924575440376 1107 24-25 0.2273445446276341 1107 26-27 0.23680029998648422 1107 28-29 0.2205077328209768 1107 30-31 0.28435543458291335 1107 32-33 0.2013830388217812 1107 34-35 0.28263932207431 1107 36-37 0.28530029485735753 1107 38-39 0.4928136910748435 1107 40-41 0.33691781068807103 1107 42-43 0.30895275958643964 1107 44-45 0.265131596256019 1107 46-47 0.38760751253796855 1107 48-49 0.23373660280500985 1107 50-51 0.28120194818101396 1107 52-53 0.3244009455733732 1107 54-55 0.43528077909714114 1107 56-57 0.46814187324719114 1107 58-59 0.49984780084261615 1107 60-61 0.5486467770358914 1107 62-63 0.3544869686445935 1107 64-65 0.6082752073587088 1107 66-67 0.35646548714455406 1107 68-69 0.5197533883271106 1107 70-71 0.5048819902461972 1107 72-73 0.4873347816750453 1107 74-75 0.538457299371629 1107 76-77 0.44350966589218643 1107 78-79 0.40852205240415884 1107 80-81 0.40729575695141307 1107 82-83 0.4224960519699863 1107 84-85 0.4512890647511796 1107 86-87 0.36127575614649743 1107 88-89 0.4071468581295292 1107 90-91 0.38632547527571504 1107 92-93 0.3728826243321066 1107 94-95 0.19816258855293967 1107 96-97 0.11567167624312447 1107 98-99 0.17195018759571923 1107 100-101 0.3446501740693044 1108 1 0.08107583236824922 1108 2 0.09491650284328301 1108 3 0.08664481928881429 1108 4 0.1861895904923827 1108 5 0.1865392235576948 1108 6 0.2920000493834536 1108 7 0.1901697307501351 1108 8 0.15477005733015403 1108 9 0.2660994351284103 1108 10-11 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-1.6880455952779414 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 9.0 7 63.0 8 304.0 9 402.0 10 440.0 11 553.0 12 667.0 13 896.0 14 1085.0 15 1356.0 16 2201.0 17 3487.0 18 4629.0 19 5958.0 20 7878.0 21 9553.0 22 9399.0 23 8451.0 24 7714.0 25 7422.0 26 7939.0 27 8977.0 28 10454.0 29 12304.0 30 14790.0 31 18024.0 32 22697.0 33 29477.0 34 40910.0 35 60404.0 36 100264.0 37 201707.0 38 345401.0 39 121141.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.382184259843484 23.7108120579732 13.880418506973546 21.02658517520977 2 13.04004281869687 24.43290154051362 41.815298277563365 20.711757363226152 3 17.91743862736389 30.47414428567538 32.04053979185467 19.567877295106058 4 9.669599096788978 19.100151599686658 45.64296489161656 25.587284411907806 5 9.693512291639655 42.763030566093754 36.845606182742664 10.69785095952393 6 24.060889586682492 43.71103081476584 19.979548220375907 12.248531378175754 7 23.660427990757007 34.84638359922743 26.47332498922558 15.019863420789973 8 22.663783503625236 36.38484780767563 23.274468232939043 17.676900455760098 9 25.467878537492254 16.877617321740438 24.474376068055058 33.18012807271225 10-11 22.043137728115962 27.99041029062585 32.005795895638194 17.96065608561999 12-13 23.701659948564973 27.552855169745914 30.166670891887858 18.578813989801255 14-15 21.64654301372127 28.718039732711315 29.541766674554616 20.093650579012802 16-17 18.497544951379766 31.667993191807092 30.297021251448662 19.537440605364477 18-19 18.042129224665135 31.731736517963704 31.96733991297769 18.258794344393472 20-21 19.64036517372556 30.327535452235665 30.939625997775067 19.092473376263708 22-23 19.558895335814423 29.862708914595366 30.84799199252486 19.73040375706535 24-25 20.21381383182615 30.23360759060659 30.429108446742887 19.123470130824373 26-27 19.6575361081459 29.09349833130741 32.748595833376285 18.5003697271704 28-29 18.6687385133875 29.845886094040974 32.545644201562695 18.939731191008836 30-31 19.72351530408068 29.81041290418115 30.872265588756548 19.59380620298162 32-33 19.313956942951428 30.09283947655255 31.69002336450788 18.903180215988147 34-35 19.976935397557458 29.75549272120135 31.57615301925112 18.691418861990076 36-37 20.1196247808113 28.51022911838111 31.82980741384982 19.54033868695777 38-39 19.080499305062872 30.96528313416176 31.634306557713522 18.319911003061847 40-41 18.65571137100833 30.38637192210331 32.358250164245085 18.599666542643273 42-43 18.78373350402951 30.275781792553534 30.083748593021763 20.85673611039519 44-45 19.643793077721842 29.848435864313476 30.594361707169327 19.91340935079536 46-47 20.29390689755237 29.84869842706843 30.920280947045857 18.937113728333337 48-49 19.124605622751396 30.08312231873889 30.892114518663945 19.90015753984576 50-51 18.053289805932 29.92919919928838 31.1412860246375 20.876224970142122 52-53 19.002273486124608 30.729391373508257 30.148404995666873 20.119930144700263 54-55 19.194176542207032 29.544687093730243 30.223984196634092 21.037152167428633 56-57 20.101767561613194 29.482051339792566 29.613928813374844 20.802252285219403 58-59 20.48192092939481 29.12154403818444 29.812548781219984 20.58398625120077 60-61 20.425505357412675 30.410883567191256 30.283623313402774 18.879987761993295 62-63 18.836192824848805 30.076824613939284 31.275251012858906 19.811731548353002 64-65 18.631833115772224 30.68672045165123 30.118965729198827 20.56248070337772 66-67 19.43154930000319 29.77683790209239 29.303277534990457 21.48833526291396 68-69 19.05474975441283 29.963417597646114 29.752874097050796 21.22895855089026 70-71 20.10194801570495 28.898716597555328 30.674927195064694 20.32440819167503 72-73 19.82563562431797 28.643318353559856 30.925971344442093 20.605074677680076 74-75 18.905863940438454 30.803484904301225 29.095572069144332 21.195079086115992 76-77 18.600347825433523 29.976860168633813 30.523489958102207 20.89930204783046 78-79 19.254440363347463 29.817880460618042 29.755138873833097 21.172540302201398 80-81 19.817028702584643 29.64009329125566 29.320430512975282 21.222447493184422 82-83 19.80535120037335 29.461807794863613 30.585470756949313 20.14737024781372 84-85 19.213588084110725 29.492182460999082 30.114517764021077 21.179711690869116 86-87 18.108537645088663 30.13013956555117 30.861286539176696 20.90003625018348 88-89 19.302848206508457 29.174544995715095 29.510301359864872 22.012305437911575 90-91 19.27450312514368 28.928199108388675 30.374979708382476 21.422318058085168 92-93 20.528277158920165 29.936081195704574 29.738602742542454 19.79703890283281 94-95 19.63189435556642 28.442104048054667 30.328336945754558 21.597664650624356 96-97 19.263834353583885 28.571361570166538 30.98117586142853 21.183628214821056 98-99 19.32351834986724 29.30684219461204 29.653449234800828 21.716190220719888 100-101 18.787055498140308 28.45252676061093 30.33585105963954 22.42456668160922 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 236.0 1 153.0 2 121.0 3 248.0 4 216.5 5 153.0 6 264.5 7 479.0 8 690.5 9 729.0 10 607.0 11 516.0 12 557.0 13 616.0 14 647.0 15 697.5 16 760.0 17 843.5 18 951.0 19 1055.0 20 1210.0 21 1390.0 22 1593.5 23 1936.0 24 2283.5 25 2756.5 26 3760.5 27 5379.0 28 7103.5 29 9712.5 30 14645.0 31 20116.5 32 30758.0 33 43929.0 34 55675.0 35 65239.5 36 76449.0 37 82501.5 38 71720.5 39 61834.0 40 53317.0 41 43211.5 42 36562.0 43 32303.5 44 31148.0 45 31766.0 46 31170.5 47 34286.5 48 46880.0 49 42758.0 50 25103.0 51 15609.0 52 10823.0 53 9238.5 54 8041.5 55 7114.5 56 6312.0 57 5667.0 58 5268.0 59 4602.0 60 3737.5 61 3030.0 62 2323.5 63 1657.0 64 1253.0 65 981.0 66 709.0 67 473.5 68 354.0 69 253.5 70 178.0 71 123.5 72 81.5 73 59.0 74 41.0 75 26.0 76 19.5 77 16.0 78 15.5 79 14.0 80 12.0 81 9.5 82 6.0 83 3.5 84 4.5 85 5.0 86 3.0 87 2.5 88 2.0 89 2.0 90 2.5 91 2.0 92 1.5 93 1.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.481067063541527 2 0.36700927738722383 3 0.19437621075104758 4 0.22089909775323968 5 0.24564129622878286 6 0.19297040401948262 7 0.18556648856657387 8 0.1845355636300929 9 0.18584764991288685 10-11 0.19789072757995996 12-13 0.1846292840788639 14-15 0.1955945765850705 16-17 0.08758175937649659 18-19 0.03561377053297882 20-21 3.7488179508398756E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.1325675747865751 46-47 0.20393569652568927 48-49 0.08655083444001564 50-51 0.06574489481285432 52-53 0.07488263856802652 54-55 0.0844421243426682 56-57 0.11630707692480714 58-59 0.09550113729764584 60-61 0.13776905969336545 62-63 0.24043981132199255 64-65 0.13139606917693764 66-67 0.10693503204770745 68-69 0.1115273340374863 70-71 0.0908619750834815 72-73 0.07380485340716006 74-75 0.1453135558194307 76-77 0.2238512918895261 78-79 0.14259566280507177 80-81 0.08242713469409177 82-83 0.09189290001996246 84-85 0.1029519129749401 86-87 0.07511693968995402 88-89 0.09854705188270324 90-91 0.12108681981212799 92-93 0.17089923833391282 94-95 0.13744103812266695 96-97 0.08669141511317213 98-99 0.07591356350450748 100-101 0.06532315279338483 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1067003.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.29882192024707 #Duplication Level Percentage of deduplicated Percentage of total 1 69.77752926861791 13.46624111389885 2 11.370479790435509 4.388737292467662 3 4.769515851020721 2.761381111639336 4 2.7618724051699486 2.132035348552772 5 1.8021877054923907 1.7390049797578162 6 1.223004307796398 1.4161525406314632 7 0.943689726621343 1.274846998742236 8 0.7412979527740885 1.1444941744344688 9 0.5911785384546624 1.0268144403037618 >10 4.63703915630314 18.022585151066707 >50 0.6818613049646187 9.277516941989502 >100 0.6084300875045789 23.691814431076835 >500 0.05944431889412553 7.719342180616609 >1k 0.03147052176747822 10.189111357807633 >5k 4.995320915472734E-4 0.6198764904695065 >10k+ 4.995320915472734E-4 1.1300454465448764 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11722 1.0985911004936255 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6430 0.60262248559751 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4406 0.4129322972850123 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3531 0.33092690461039004 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3516 0.32952109787882505 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 3513 0.3292399365325121 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 3369 0.31574419190948855 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3195 0.2994368338233351 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 3194 0.2993431133745641 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2740 0.2567940296325315 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2640 0.2474219847554318 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2635 0.2469533825115768 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2288 0.2144323867880409 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 2249 0.210777289285972 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 2216 0.20768451447652914 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2184 0.2046854601158572 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2047 0.19184575863423065 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2029 0.1901587905563527 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1903 0.1783500140112071 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1883 0.17647560503578713 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 1877 0.17591328234316117 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1814 0.17000889407058836 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1734 0.16251125816890863 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 1695 0.15885616066683975 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1532 0.14357972751716724 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1527 0.14311112527331224 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1525 0.14292368437577024 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 1509 0.1414241571954343 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 1436 0.13458256443515154 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 1410 0.13214583276710562 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1327 0.12436703551911288 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 1327 0.12436703551911288 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1323 0.12399215372402889 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1315 0.1232423901338609 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1312 0.12296122878754792 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 1289 0.120805658465815 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1261 0.11818148590022708 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 1243 0.11649451782234914 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1218 0.11415150660307422 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 1210 0.11340174301290623 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1204 0.11283942032028027 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 1201 0.11255825897396726 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1197 0.11218337717888327 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 1184 0.11096501134486031 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1179 0.11049640910100533 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1152 0.10796595698418841 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 1136 0.10646642980385247 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1134 0.10627898890631048 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1106 0.10365481634072257 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1103 0.10337365499440959 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 1102 0.10327993454563858 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1093 0.10243645050669961 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 1086 0.10178040736530264 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 1073 0.10056204153127968 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 9.372044877099689E-5 0.0 3 0.0 0.0 0.0 9.372044877099689E-5 0.0 4 0.0 0.0 0.0 1.8744089754199378E-4 0.0 5 0.0 0.0 0.0 1.8744089754199378E-4 0.0 6 0.0 0.0 0.0 1.8744089754199378E-4 0.0 7 0.0 0.0 0.0 1.8744089754199378E-4 0.0 8 0.0 0.0 0.0 2.811613463129907E-4 0.0 9 0.0 0.0 0.0 4.686022438549845E-4 0.0 10-11 0.0 0.0 0.0 6.560431413969783E-4 0.0 12-13 0.0 0.0 0.0 0.0013120862827939565 0.0 14-15 0.0 0.0 0.0 0.0018744089754199377 0.0 16-17 0.0 0.0 0.0 0.004451721316622352 0.0 18-19 0.0 0.0 0.0 0.007357055228523256 0.0 20-21 0.0 0.0 0.0 0.012745981032855578 0.0 22-23 0.0 0.0 0.0 0.026382306329035626 0.0 24-25 0.0 0.0 0.0 0.04442349271745252 0.0 26-27 0.0 0.0 0.0 0.05571680679435766 0.0 28-29 0.0 0.0 0.0 0.06729128221757577 0.0 30-31 0.0 0.0 0.0 0.09133057732733647 0.0 32-33 0.0 0.0 0.0 0.13678499498126998 0.0 34-35 0.0 0.0 0.0 0.2056226646035672 0.0 36-37 0.0 0.0 0.0 0.29386046712146074 0.0 38-39 0.0 0.0 0.0 0.4292865155955513 0.0 40-41 0.0 0.0 0.0 0.5953122905933723 0.0 42-43 0.0 0.0 0.0 0.736877028461963 0.0 44-45 0.0 0.0 0.0 0.8877669509832681 0.0 46-47 0.0 0.0 0.0 1.0741300633643953 0.0 48-49 0.0 0.0 0.0 1.2567912180190683 0.0 50-51 0.0 0.0 0.0 1.4690680344853764 0.0 52-53 0.0 0.0 0.0 1.6593205454905 0.0 54-55 0.0 0.0 0.0 1.8535561755683911 0.0 56-57 0.0 0.0 0.0 2.098353987758235 0.0 58-59 0.0 0.0 0.0 2.3544451140249842 0.0 60-61 0.0 0.0 0.0 2.62857742668015 0.0 62-63 0.0 0.0 0.0 2.8784361431036274 0.0 64-65 0.0 0.0 0.0 3.1284823004246474 0.0 66-67 0.0 0.0 0.0 3.4180316269026423 0.0 68-69 0.0 0.0 0.0 3.742444960323448 0.0 70-71 0.0 0.0 0.0 4.120653831338807 0.0 72-73 0.0 0.0 0.0 4.48724136670656 0.0 74-75 0.0 0.0 0.0 4.8312422739205045 0.0 76-77 0.0 0.0 0.0 5.189394968898869 0.0 78-79 0.0 0.0 0.0 5.658231513875781 0.0 80-81 0.0 0.0 0.0 6.173553401443107 0.0 82-83 0.0 0.0 0.0 6.688875289010435 0.0 84-85 0.0 0.0 0.0 7.162819598445365 0.0 86-87 0.0 0.0 0.0 7.642152833684628 0.0 88-89 0.0 0.0 0.0 8.185778296780796 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 150 2.5465852E-11 34.83972 9 TGGTCGA 35 2.0454927E-4 33.952324 76-77 ATGGGTA 325 0.0 33.637337 5 TGGGTAT 300 0.0 30.087435 6 ATGGGAT 955 0.0 28.867008 5 GTACATG 6270 0.0 28.41723 1 CATGGGT 545 0.0 27.908121 4 CATGGGG 1855 0.0 27.416817 4 TAGGGTC 175 4.9258233E-9 27.160583 4 CGTAATC 35 0.008399099 27.138914 68-69 CGCTATG 35 0.008456932 27.100761 34-35 ACATGGG 6280 0.0 27.017485 3 ACAATGC 125 7.3797237E-6 26.604874 8 ATGCCGT 45 8.925801E-4 26.401161 72-73 CCGTACG 45 8.941718E-4 26.393106 78-79 TACGTGT 45 8.9760806E-4 26.375772 82-83 ACCTGTG 345 0.0 26.162987 7 ATGGGGA 585 0.0 25.999872 5 GTAAATG 1685 0.0 25.949217 3 GGCATTC 210 1.2623786E-9 24.884346 7 >>END_MODULE