##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031607_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1067003 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46090592060191 34.0 31.0 34.0 31.0 34.0 2 32.6535173753026 34.0 31.0 34.0 31.0 34.0 3 32.75778980940073 34.0 31.0 34.0 31.0 34.0 4 36.20290570879369 37.0 35.0 37.0 35.0 37.0 5 35.946830515003235 37.0 35.0 37.0 35.0 37.0 6 36.03049663403008 37.0 37.0 37.0 35.0 37.0 7 35.99057640887607 37.0 36.0 37.0 35.0 37.0 8 36.0942387228527 37.0 37.0 37.0 35.0 37.0 9 37.96382015795644 39.0 39.0 39.0 35.0 39.0 10-11 37.93362811538486 39.0 38.0 39.0 35.0 39.0 12-13 37.974284983266216 39.0 38.5 39.0 35.0 39.0 14-15 39.333973756399935 41.0 39.0 41.0 36.0 41.0 16-17 39.323256354480726 41.0 39.0 41.0 36.0 41.0 18-19 39.42224061225695 41.0 39.0 41.0 36.0 41.0 20-21 39.458911080849816 41.0 39.0 41.0 36.0 41.0 22-23 39.37632790160852 41.0 39.0 41.0 36.0 41.0 24-25 39.282821604062974 41.0 39.0 41.0 36.0 41.0 26-27 39.15587397598695 41.0 39.0 41.0 36.0 41.0 28-29 39.09765342740367 41.0 39.0 41.0 35.0 41.0 30-31 38.968129424190934 40.0 38.5 41.0 35.0 41.0 32-33 38.77658263378829 40.0 38.0 41.0 35.0 41.0 34-35 38.628686142400724 40.0 38.0 41.0 35.0 41.0 36-37 38.45123490749323 40.0 38.0 41.0 34.5 41.0 38-39 38.30561769741978 40.0 38.0 41.0 34.0 41.0 40-41 38.25230856895435 40.0 38.0 41.0 34.0 41.0 42-43 38.09803393242568 40.0 38.0 41.0 33.5 41.0 44-45 37.97736604301956 40.0 38.0 41.0 33.0 41.0 46-47 37.89785220847551 40.0 38.0 41.0 33.0 41.0 48-49 37.91861222508278 40.0 38.0 41.0 33.0 41.0 50-51 37.82523760476775 40.0 38.0 41.0 33.0 41.0 52-53 37.81858673312071 40.0 38.0 41.0 33.0 41.0 54-55 37.73851526190648 40.0 38.0 41.0 33.0 41.0 56-57 37.62737171310671 40.0 38.0 41.0 32.5 41.0 58-59 37.472320134057725 40.0 38.0 41.0 32.0 41.0 60-61 37.18471175807379 40.0 37.0 41.0 31.0 41.0 62-63 37.03834009838773 40.0 37.0 41.0 31.0 41.0 64-65 36.78062198513031 39.0 36.0 41.0 31.0 41.0 66-67 36.4780994055312 39.0 36.0 41.0 30.5 41.0 68-69 36.13849398736461 39.0 35.5 40.5 30.0 41.0 70-71 35.727186802661286 37.5 35.0 40.0 29.5 41.0 72-73 35.3291382498456 37.0 35.0 39.5 29.0 41.0 74-75 34.93959342194914 37.0 35.0 39.0 29.0 41.0 76-77 34.27628741437466 36.0 34.0 39.0 28.5 40.0 78-79 34.18770378340079 36.0 34.0 37.5 29.0 39.5 80-81 33.84899292691773 35.0 34.0 37.0 28.0 39.0 82-83 33.51316631724559 35.0 34.0 37.0 27.5 39.0 84-85 33.179202870095025 35.0 34.0 36.0 27.0 37.5 86-87 32.97224890651666 35.0 34.0 36.0 27.0 37.0 88-89 32.72128475739993 35.0 34.0 35.5 26.5 37.0 90-91 32.545245421053174 35.0 34.0 35.0 26.0 36.0 92-93 32.40822565634773 35.0 34.0 35.0 26.0 36.0 94-95 32.24642105036256 35.0 33.0 35.0 25.0 36.0 96-97 32.138542721997965 35.0 33.0 35.0 25.0 36.0 98-99 32.0851946995463 35.0 33.0 35.0 25.0 35.5 100-101 31.651755430865705 34.5 32.5 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12111759992225757 1101 2 0.11527837889074988 1101 3 0.08956268444018178 1101 4 0.107346968878403 1101 5 0.0640014727413245 1101 6 0.19220590693772266 1101 7 0.2782814308659667 1101 8 0.30331640008053995 1101 9 0.3927341433467575 1101 10-11 0.15016713077716304 1101 12-13 0.24290179118285948 1101 14-15 0.19701298618106478 1101 16-17 0.2854867667748735 1101 18-19 0.26902579959273965 1101 20-21 0.08444218583661467 1101 22-23 0.13744837259916665 1101 24-25 0.1939357537696793 1101 26-27 0.2097234735915734 1101 28-29 0.17100754895975 1101 30-31 0.06351187302826844 1101 32-33 0.14962878767565968 1101 34-35 0.1445796685849814 1101 36-37 0.20035450626284756 1101 38-39 0.1987419383790865 1101 40-41 0.260530489457679 1101 42-43 0.2528331618317239 1101 44-45 0.32914368686915196 1101 46-47 0.34539868938120577 1101 48-49 0.18338875121722964 1101 50-51 0.3777987777091667 1101 52-53 0.19975775464980217 1101 54-55 0.41670568904660144 1101 56-57 0.4522862400016834 1101 58-59 0.33911667129419953 1101 60-61 0.2790978233805106 1101 62-63 0.4347454378257467 1101 64-65 0.32258489802936197 1101 66-67 0.5803332441373854 1101 68-69 0.48286636246157144 1101 70-71 0.23403745834222178 1101 72-73 0.24508018320740632 1101 74-75 0.26882228395069774 1101 76-77 0.3202214924030997 1101 78-79 0.5126386216383665 1101 80-81 0.3565466856164008 1101 82-83 0.4352349491735481 1101 84-85 0.29674827165862183 1101 86-87 0.3506374311776881 1101 88-89 0.4527170367907374 1101 90-91 0.4104104822256147 1101 92-93 0.2650283648471188 1101 94-95 0.3274803637952033 1101 96-97 0.3485688282122439 1101 98-99 0.35936740612187634 1101 100-101 0.45702089757083186 1102 1 -0.0010480765714078188 1102 2 0.10658877010249768 1102 3 0.07213563963952652 1102 4 0.10525004401945637 1102 5 0.03185858890917359 1102 6 -0.029032369787607593 1102 7 0.08318671054313143 1102 8 0.011311766818202784 1102 9 0.01140917977765099 1102 10-11 0.07156458248287834 1102 12-13 -0.02450171365591558 1102 14-15 0.13295663973261185 1102 16-17 0.10989284979994807 1102 18-19 0.11337807699064228 1102 20-21 0.14438763290906564 1102 22-23 0.1382726449154248 1102 24-25 0.12185564316922637 1102 26-27 0.2704879618776417 1102 28-29 -0.2055929816235249 1102 30-31 -0.16357477645048135 1102 32-33 0.02752887361520351 1102 34-35 0.10980778202517882 1102 36-37 0.024080545944492826 1102 38-39 -0.028584594520815187 1102 40-41 -0.06864561187624929 1102 42-43 0.11885341367194968 1102 44-45 0.08803842961099662 1102 46-47 0.12008388909723067 1102 48-49 0.11233991513756081 1102 50-51 0.26141870073723084 1102 52-53 -0.03540646460812269 1102 54-55 -0.015901268319908013 1102 56-57 -0.215334729246905 1102 58-59 -0.13072452737560525 1102 60-61 0.3157327297811463 1102 62-63 0.07803580138737942 1102 64-65 0.20423194806112832 1102 66-67 0.13459322993868028 1102 68-69 -0.12794259019695176 1102 70-71 -0.04716494796499404 1102 72-73 0.1568113045316224 1102 74-75 0.22984010706469604 1102 76-77 0.11410483881418543 1102 78-79 0.08775277177101515 1102 80-81 -0.0028113833594218818 1102 82-83 -0.19785850073863998 1102 84-85 -0.16433979769085738 1102 86-87 0.0510051029633658 1102 88-89 0.1175916771524328 1102 90-91 0.08137113686074571 1102 92-93 -0.04060926919988361 1102 94-95 0.14962959968056566 1102 96-97 0.02620586441894801 1102 98-99 0.09962821395358645 1102 100-101 0.09380900054882702 1103 1 0.08341719469798647 1103 2 0.16514963649841263 1103 3 0.10924904170138205 1103 4 0.02149705016336867 1103 5 0.12895741219356438 1103 6 0.14914111766422877 1103 7 0.18606289506880103 1103 8 0.13820128051183644 1103 9 0.28074168003799116 1103 10-11 0.1940617187944511 1103 12-13 0.09229720334919733 1103 14-15 0.21824665327005022 1103 16-17 0.20017921864302224 1103 18-19 0.1578016946101286 1103 20-21 0.027349207417024957 1103 22-23 -0.10439735404323613 1103 24-25 0.18928040626409626 1103 26-27 0.10606642694063595 1103 28-29 0.01832246146717864 1103 30-31 0.08589101548952982 1103 32-33 0.1744683716887252 1103 34-35 0.048066659459315986 1103 36-37 0.16947469360668066 1103 38-39 0.08806020397965852 1103 40-41 0.13196253141230585 1103 42-43 0.08018841315128356 1103 44-45 0.34636395288497823 1103 46-47 0.17939868655636104 1103 48-49 0.04870146881871307 1103 50-51 0.12032529242733148 1103 52-53 0.042610821656587916 1103 54-55 0.05294804960156796 1103 56-57 0.07928570186836481 1103 58-59 0.1891921653245916 1103 60-61 0.17689486661338094 1103 62-63 0.04817221709080144 1103 64-65 0.0680640838520219 1103 66-67 0.1073663216808356 1103 68-69 0.18456183550335936 1103 70-71 0.09408466153204387 1103 72-73 -0.018702597374037566 1103 74-75 0.11255175967325926 1103 76-77 0.049572921704942985 1103 78-79 0.05748306438790962 1103 80-81 0.10960140431109977 1103 82-83 0.1295787584511956 1103 84-85 0.03560813524676831 1103 86-87 -0.1387418511134868 1103 88-89 0.11032936329218046 1103 90-91 0.15608528868829552 1103 92-93 -0.10343546934566916 1103 94-95 0.10255264456236546 1103 96-97 0.13756689742945838 1103 98-99 -0.007484146767019695 1103 100-101 -0.03866626759654679 1104 1 0.11061449509116272 1104 2 0.09677714029086815 1104 3 0.01627978378367345 1104 4 0.08818616195557638 1104 5 -0.024791697741115115 1104 6 0.08030423954900101 1104 7 0.1107788481352685 1104 8 0.14625443676087002 1104 9 0.12273186610033804 1104 10-11 0.09593395185224551 1104 12-13 0.033618679464481716 1104 14-15 -0.10355646428048715 1104 16-17 0.13176221916931752 1104 18-19 0.03281910893166895 1104 20-21 0.016877395398829265 1104 22-23 -0.07596363682085894 1104 24-25 -0.06064026182667703 1104 26-27 0.05963210602178748 1104 28-29 -0.12102500455554832 1104 30-31 -0.16892694680265663 1104 32-33 -0.021011257424923713 1104 34-35 0.09302850274590213 1104 36-37 0.062299219163165276 1104 38-39 -0.0391456100818246 1104 40-41 -0.21006248329312882 1104 42-43 -0.22879195897342441 1104 44-45 -0.1255467464741784 1104 46-47 0.14481771383105269 1104 48-49 0.08216792496558156 1104 50-51 0.14629585061437922 1104 52-53 0.14479419059252763 1104 54-55 0.1203047103860655 1104 56-57 -0.007263476175651817 1104 58-59 0.08415671750564258 1104 60-61 0.1495717961202061 1104 62-63 0.22548870884028105 1104 64-65 0.11028026910945243 1104 66-67 0.11040608825152987 1104 68-69 -0.0160588883132462 1104 70-71 -0.03646470226474463 1104 72-73 0.08317698839730525 1104 74-75 -0.0446705399459546 1104 76-77 -0.030571655862310365 1104 78-79 0.08508282910107567 1104 80-81 0.09265339960536068 1104 82-83 0.07187737149723006 1104 84-85 0.3120217911707286 1104 86-87 0.4116377810960401 1104 88-89 0.16597005553081345 1104 90-91 0.2724419804315872 1104 92-93 0.29703200844139843 1104 94-95 0.033845406396366684 1104 96-97 0.09944501265808725 1104 98-99 0.0817023335277014 1104 100-101 0.08475289899272198 1105 1 0.020041256630598525 1105 2 0.11273079708959699 1105 3 0.04187200655898948 1105 4 0.006762240742922643 1105 5 0.042178034721573 1105 6 0.012170558739263981 1105 7 -0.02900475995819818 1105 8 0.09613267361093847 1105 9 0.069067014900277 1105 10-11 0.0946126142633048 1105 12-13 0.1758778129207954 1105 14-15 0.18209219900901985 1105 16-17 0.10572528028026795 1105 18-19 -0.028671891819897155 1105 20-21 0.034756325249588826 1105 22-23 -0.0034614894947679886 1105 24-25 0.08155359779676274 1105 26-27 0.11172306480288086 1105 28-29 -0.08204066539514798 1105 30-31 -0.22009797156523092 1105 32-33 0.09831845961605978 1105 34-35 0.0752465685907282 1105 36-37 0.014105169208555424 1105 38-39 -0.012368697741528933 1105 40-41 -0.004975760600764545 1105 42-43 -0.12448681417532015 1105 44-45 -0.109420449538014 1105 46-47 0.1708833244037109 1105 48-49 0.23493553547966428 1105 50-51 0.2996747170214107 1105 52-53 0.22133538509147144 1105 54-55 0.09145550636080912 1105 56-57 0.16485569066182393 1105 58-59 0.13440170789147743 1105 60-61 0.30270603816289565 1105 62-63 0.30930275040080346 1105 64-65 0.2076017238252703 1105 66-67 0.146005948675338 1105 68-69 0.13363658180475824 1105 70-71 0.20972989428196342 1105 72-73 0.3408823508561909 1105 74-75 0.24305590535091426 1105 76-77 0.19306015579767433 1105 78-79 0.27564337881655376 1105 80-81 0.2609201902313103 1105 82-83 0.3979470083063035 1105 84-85 0.3198455005789498 1105 86-87 0.3657258751489252 1105 88-89 0.5108672278927742 1105 90-91 0.3896524330293616 1105 92-93 0.3406422492347403 1105 94-95 0.23721487825176268 1105 96-97 0.24662347497739745 1105 98-99 0.2745584480387393 1105 100-101 0.22820343240804775 1106 1 0.2098685979948982 1106 2 0.1840254939625794 1106 3 0.1554168353294827 1106 4 0.09795962154655058 1106 5 0.15839696537455694 1106 6 0.0470253924015438 1106 7 0.14815590193568084 1106 8 0.20799644467076206 1106 9 0.07143382666361475 1106 10-11 0.06671925749156316 1106 12-13 0.119710375957645 1106 14-15 0.11751947349252134 1106 16-17 0.17182541844784538 1106 18-19 0.08024381465929764 1106 20-21 0.09817219384691356 1106 22-23 0.17272646360282096 1106 24-25 0.1361388295691981 1106 26-27 0.19194781589223453 1106 28-29 0.20892466617880245 1106 30-31 0.1962185688903091 1106 32-33 0.3522654995197101 1106 34-35 0.05589745877179553 1106 36-37 0.2035534817677913 1106 38-39 0.2779487261730864 1106 40-41 0.32693880370816686 1106 42-43 0.33231124537795864 1106 44-45 0.40556615735770407 1106 46-47 0.322090398403013 1106 48-49 0.3286991277011566 1106 50-51 0.5744316245530783 1106 52-53 0.4083417579722166 1106 54-55 0.5111262540360855 1106 56-57 0.5177919494272203 1106 58-59 0.5154454097432435 1106 60-61 0.5529040256385684 1106 62-63 0.6346376909819682 1106 64-65 0.6735535267257688 1106 66-67 0.7089750038824931 1106 68-69 0.5122293060333476 1106 70-71 0.6086975350507728 1106 72-73 0.6767329400736983 1106 74-75 0.6473621470684918 1106 76-77 0.5888165018470133 1106 78-79 0.7315003623799257 1106 80-81 0.6772214791551932 1106 82-83 0.7259611346138399 1106 84-85 0.6497310463069965 1106 86-87 0.5511359210503812 1106 88-89 0.7141644960610165 1106 90-91 0.5826357117019612 1106 92-93 0.5834884335839519 1106 94-95 0.5954247777385149 1106 96-97 0.5059859383596006 1106 98-99 0.653412142919997 1106 100-101 0.7265596562191909 1107 1 0.11164294128411711 1107 2 0.1081259766646312 1107 3 0.1476424525341784 1107 4 0.09451739348620691 1107 5 0.15462725261502186 1107 6 0.1520305022024786 1107 7 0.1265221587439811 1107 8 0.10295078914516154 1107 9 0.09591928582315035 1107 10-11 0.16080353230008626 1107 12-13 0.07692008156448793 1107 14-15 -0.008835559427481599 1107 16-17 0.0891417697068917 1107 18-19 -0.07170908576352275 1107 20-21 0.02443355574661865 1107 22-23 0.05395348119446197 1107 24-25 0.01956675226617932 1107 26-27 0.09357868969419059 1107 28-29 0.05745363609906917 1107 30-31 0.1283512636111439 1107 32-33 0.21360639861595843 1107 34-35 0.1358297162225881 1107 36-37 0.17430441977754185 1107 38-39 0.1829662076182288 1107 40-41 0.1751085800932941 1107 42-43 -0.004636721981697178 1107 44-45 0.10908438266094578 1107 46-47 0.3323048021157504 1107 48-49 0.21642281794429863 1107 50-51 0.1908165677422744 1107 52-53 0.011108535845060885 1107 54-55 0.25056539230428143 1107 56-57 0.24703331812612817 1107 58-59 0.14613519648312234 1107 60-61 0.48369611236067556 1107 62-63 0.3267973953932355 1107 64-65 0.19374945614293893 1107 66-67 0.3867555309145345 1107 68-69 0.35324536110067584 1107 70-71 0.29809805091715447 1107 72-73 0.4969823066767063 1107 74-75 0.38541701271138606 1107 76-77 0.2320661065261973 1107 78-79 0.3518411128085148 1107 80-81 0.2952791933951673 1107 82-83 0.2556307285432098 1107 84-85 0.1907555224094466 1107 86-87 0.14612519722603423 1107 88-89 0.17983162397862174 1107 90-91 0.30694713613565483 1107 92-93 0.37911458952697075 1107 94-95 0.3573506788672489 1107 96-97 0.4053774405994943 1107 98-99 0.3240670187513146 1107 100-101 0.5244782050565142 1108 1 0.061481948851579205 1108 2 0.06098869997012457 1108 3 0.12060879939522806 1108 4 0.04625235254346194 1108 5 -0.004812461030923032 1108 6 0.04095389595564569 1108 7 0.035451109596344565 1108 8 0.16319628217426896 1108 9 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23.0 14 96.0 15 313.0 16 968.0 17 2193.0 18 3445.0 19 4644.0 20 6278.0 21 8213.0 22 8426.0 23 7070.0 24 6156.0 25 5720.0 26 6292.0 27 7277.0 28 8700.0 29 10536.0 30 12794.0 31 16242.0 32 20098.0 33 26108.0 34 35711.0 35 53811.0 36 91954.0 37 203517.0 38 383103.0 39 137196.0 40 116.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.24833688537425 23.567705525337946 14.056518237300086 21.12743935198772 2 13.335757584845679 24.542453767399316 41.49856231888405 20.62322632887096 3 17.998457352738573 30.23569888200248 32.110212118684494 19.655631646574452 4 9.749431292547612 19.23960509629363 45.136953752625615 25.874009858533142 5 9.53558706020508 42.76576541959113 36.926419138465405 10.772228381738383 6 23.891779123395153 43.91393463748462 19.79535202806365 12.398934211056577 7 23.757290279408775 34.7180842040744 26.22616806138314 15.29845745513368 8 22.618867988187475 36.60842565578541 23.14220297412472 17.630503381902393 9 25.24903866249673 17.03022390752416 24.21839488736208 33.50234254261703 10-11 21.987004722573413 28.025647538010674 31.951643997252116 18.0357037421638 12-13 23.809351988701064 27.50779519832653 30.032342926870868 18.65050988610154 14-15 21.638083491798994 28.756292156629364 29.419458052132935 20.186166299438707 16-17 18.43720214469875 31.7229192420265 30.23548199958201 19.604396613692746 18-19 18.066631490258228 31.651035657819143 31.90112867536455 18.38120417655808 20-21 19.585043341021535 30.221049050471272 31.049678398280044 19.144229210227152 22-23 19.50757185246556 29.661342595629904 30.980910571726877 19.85017498017766 24-25 20.137056784282706 30.25052413160975 30.64902347978403 18.963395604323512 26-27 19.620329089983816 28.99541988166856 32.898829712756196 18.485421315591427 28-29 18.48935399371115 29.76874481221325 32.740037223175484 19.001863970900118 30-31 19.703677388634137 29.70513964553393 31.070147088046614 19.521035877785316 32-33 19.29887699419636 30.041119866167136 31.814030426357952 18.845972713278552 34-35 19.947694617540908 29.59837038883677 31.83772679177097 18.616208201851354 36-37 20.140243279540922 28.443875040651246 31.91434325864126 19.501538421166575 38-39 18.967519304069434 30.718892074342808 31.956564320812593 18.357024300775162 40-41 18.624315020670046 30.235950601825863 32.48481963031032 18.654914747193775 42-43 18.809412906992765 29.959194116605108 30.41608130436372 20.815311672038412 44-45 19.561800669726324 29.627376867731392 30.856661134036173 19.954161328506107 46-47 20.26282962653338 29.761631410595847 31.01528299358109 18.960255969289683 48-49 19.1625285775427 29.943451974961928 30.93949975412883 19.95451969336654 50-51 18.07195996516917 29.75594050264746 31.234329173817642 20.937770358365725 52-53 18.931905533536455 30.59752409318437 30.39232317060027 20.078247202678906 54-55 19.137421467790254 29.574643263322304 30.19976129301025 21.088173975877194 56-57 19.9738894829724 29.4793454188976 29.67381535009742 20.872949748032575 58-59 20.40847120392351 29.04251440717599 29.924142668764752 20.624871720135747 60-61 20.299155812662107 30.59261810539338 30.218064156363273 18.890161925581243 62-63 18.777782255532554 30.209474575048056 31.127466370759972 19.885276798659422 64-65 18.440060618386266 30.88927585020848 29.960834224458598 20.709829306946652 66-67 19.27089239674115 29.961677708497543 29.10347955910152 21.663950335659788 68-69 18.88326462062431 30.156288220370513 29.505493424104714 21.454953734900464 70-71 20.07426408360614 29.0896557929078 30.474422283723662 20.3616578397624 72-73 19.84535188748298 28.82512982625166 30.579014304552093 20.750503981713265 74-75 18.842964827652782 30.887682602579375 28.85488606873645 21.41446650103139 76-77 18.62164398788007 30.008022470414797 30.18763771048441 21.18269583122072 78-79 19.250142337998273 29.833575835108167 29.457803519673103 21.45847830722046 80-81 19.836912968699476 29.72678235848217 28.95614927149773 21.48015540132063 82-83 19.890333591699054 29.549525147544948 30.074894818283294 20.4852464424727 84-85 19.244088348392648 29.628923255136115 29.58304709546271 21.543941301008527 86-87 18.220333026242663 30.16308295290641 30.328030942743368 21.288553078107558 88-89 19.262457556351762 29.288858606770553 29.00863446494527 22.440049371932414 90-91 19.348867810118623 28.963508068862037 29.841902974968203 21.845721146051137 92-93 20.431667014994336 30.10731928588767 29.19570048069218 20.265313218425813 94-95 19.54924212959101 28.619460301423704 29.727189145672504 22.104108423312773 96-97 19.11184879517677 28.672224914081777 30.394666181819545 21.821260108921905 98-99 19.186543992847255 29.50169774592949 29.07297355302656 22.238784708196697 100-101 18.651868832608717 28.783002484529096 29.67714242602879 22.887986256833393 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 212.0 1 137.0 2 122.0 3 240.0 4 218.5 5 164.5 6 239.5 7 442.5 8 627.5 9 698.0 10 624.0 11 514.5 12 557.0 13 610.5 14 640.5 15 664.5 16 722.0 17 806.5 18 868.5 19 967.5 20 1106.0 21 1280.5 22 1509.0 23 1815.0 24 2036.0 25 2420.5 26 3436.5 27 4889.5 28 6374.0 29 8803.5 30 13380.5 31 18720.0 32 29633.0 33 42981.5 34 54630.0 35 64568.0 36 76102.0 37 82327.0 38 72146.5 39 63062.0 40 54517.0 41 43725.5 42 36883.5 43 32513.5 44 31542.5 45 32388.0 46 31682.0 47 34996.0 48 47325.0 49 42364.0 50 25000.0 51 16727.0 52 12734.5 53 10941.0 54 8988.5 55 7621.0 56 6668.0 57 5935.5 58 5528.5 59 4801.0 60 3681.0 61 2719.5 62 2031.5 63 1366.5 64 943.5 65 674.5 66 451.0 67 295.0 68 233.0 69 181.5 70 122.0 71 70.0 72 44.5 73 36.0 74 20.0 75 15.5 76 12.5 77 10.5 78 8.0 79 4.0 80 3.0 81 1.0 82 1.5 83 1.0 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04498581541007851 2 6.560431413969783E-4 3 5.623226926259814E-4 4 0.010309249364809658 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0023430112192749223 24-25 0.0 26-27 0.0 28-29 0.018228627285958896 30-31 0.014901551354588505 32-33 4.6860224385498445E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.036316673898761295 50-51 0.01237109923777159 52-53 0.0 54-55 4.21742019469486E-4 56-57 0.0 58-59 0.0 60-61 4.6860224385498445E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.6860224385498445E-5 80-81 0.008247399491847726 82-83 0.003748817950839876 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1067003.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.37585625265175 #Duplication Level Percentage of deduplicated Percentage of total 1 66.1627673081393 11.49634734023875 2 12.561016327832922 4.365168281992728 3 5.394140057522832 2.8118340673856212 4 3.0918080025033845 2.1489124564918862 5 1.9747349712011437 1.715635549833773 6 1.406517262545178 1.4663665062514952 7 1.0931161229311699 1.3295680033655606 8 0.8237436935610258 1.1450601606675839 9 0.6714870045502701 1.0500895499930285 >10 5.2493923059103516 18.469987875663087 >50 0.7749838456973669 9.53046478142792 >100 0.6936722265756818 24.513103745911824 >500 0.0684139140195903 8.220111132714353 >1k 0.0330854174357035 9.96903570445388 >5k 5.607697870458221E-4 0.5656307949717505 >10k+ 5.607697870458221E-4 1.2026840486367472 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12343 1.1567914991804147 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5805 0.544047205115637 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4054 0.3799426993176214 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3823 0.35829327565152114 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3742 0.35070191930107036 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 3463 0.3245539140939622 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3243 0.30393541536434293 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 3135 0.29381360689707525 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2989 0.28013042137650973 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2913 0.27300766726991393 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2681 0.25126452315504266 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2496 0.23392624013240823 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2482 0.23261415384961429 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2391 0.22408559301145356 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 2355 0.22071165685569769 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2289 0.21452610723681187 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2072 0.19418876985350558 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 2056 0.19268924267316961 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1975 0.18509788632271887 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1847 0.17310166888003128 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 1809 0.16954029182673339 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1765 0.1654165920808095 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1747 0.16372962400293156 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 1681 0.15754407438404577 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1642 0.1538889768819769 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1634 0.1531392132918089 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 1437 0.13467628488392253 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1390 0.1302714237916857 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1359 0.1273660898797848 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1358 0.1272723694310138 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1347 0.1262414444945328 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 1345 0.12605400359699082 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 1339 0.12549168090436486 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1333 0.12492935821173887 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 1333 0.12492935821173887 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1329 0.12455447641665487 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 1321 0.12380471282648689 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 1267 0.11874380859285306 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 1253 0.11743172231005912 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1251 0.11724428141251712 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1229 0.11518243153955518 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1199 0.11237081807642527 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 1194 0.11190221583257029 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1193 0.1118084953837993 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 1153 0.10805967743295943 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1153 0.10805967743295943 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 1139 0.10674759115016545 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 1139 0.10674759115016545 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 1108 0.10384225723826457 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1101 0.1031862140968676 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 1099 0.1029987731993256 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 1097 0.1028113323017836 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 1088 0.10196784826284463 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 1084 0.10159296646776064 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 1076 0.10084320287759266 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1071 0.10037460063373768 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 9.372044877099689E-5 0.0 3 0.0 0.0 0.0 9.372044877099689E-5 0.0 4 0.0 0.0 0.0 1.8744089754199378E-4 0.0 5 0.0 0.0 0.0 1.8744089754199378E-4 0.0 6 0.0 0.0 0.0 1.8744089754199378E-4 0.0 7 0.0 0.0 0.0 1.8744089754199378E-4 0.0 8 0.0 0.0 0.0 2.811613463129907E-4 0.0 9 0.0 0.0 0.0 4.686022438549845E-4 0.0 10-11 0.0 0.0 0.0 6.560431413969783E-4 0.0 12-13 0.0 0.0 0.0 0.0012652260584084581 0.0 14-15 0.0 0.0 0.0 0.001780688526648941 0.0 16-17 0.0 0.0 0.0 0.004264280419080359 0.0 18-19 0.0 0.0 0.0 0.006794732535897275 0.0 20-21 0.0 0.0 0.0 0.012230518564615095 0.0 22-23 0.0 0.0 0.0 0.02624172565587913 0.0 24-25 0.0 0.0 0.0 0.044751514288151016 0.0 26-27 0.0 0.0 0.0 0.05627912948698364 0.0 28-29 0.0 0.0 0.0 0.06757244356388876 0.0 30-31 0.0 0.0 0.0 0.09179917957119146 0.0 32-33 0.0 0.0 0.0 0.13748789834705244 0.0 34-35 0.0 0.0 0.0 0.20609126684742218 0.0 36-37 0.0 0.0 0.0 0.2947508113847852 0.0 38-39 0.0 0.0 0.0 0.4316763870392117 0.0 40-41 0.0 0.0 0.0 0.5993422698905251 0.0 42-43 0.0 0.0 0.0 0.7416567713492839 0.0 44-45 0.0 0.0 0.0 0.8930621563388295 0.0 46-47 0.0 0.0 0.0 1.0802218925345102 0.0 48-49 0.0 0.0 0.0 1.2623207244965573 0.0 50-51 0.0 0.0 0.0 1.4741289387190102 0.0 52-53 0.0 0.0 0.0 1.664475170172905 0.0 54-55 0.0 0.0 0.0 1.8605383490018306 0.0 56-57 0.0 0.0 0.0 2.107726032635335 0.0 58-59 0.0 0.0 0.0 2.3643794815947095 0.0 60-61 0.0 0.0 0.0 2.6403393430009103 0.0 62-63 0.0 0.0 0.0 2.8917913070534946 0.0 64-65 0.0 0.0 0.0 3.143102690432923 0.0 66-67 0.0 0.0 0.0 3.4331674793791582 0.0 68-69 0.0 0.0 0.0 3.7600175444680097 0.0 70-71 0.0 0.0 0.0 4.1407100073758 0.0 72-73 0.0 0.0 0.0 4.509734274411599 0.0 74-75 0.0 0.0 0.0 4.857812021147081 0.0 76-77 0.0 0.0 0.0 5.218307727344722 0.0 78-79 0.0 0.0 0.0 5.6906119289261605 0.0 80-81 0.0 0.0 0.0 6.207058461878739 0.0 82-83 0.0 0.0 0.0 6.727816135474783 0.0 84-85 0.0 0.0 0.0 7.206540187797035 0.0 86-87 0.0 0.0 0.0 7.689528520538368 0.0 88-89 0.0 0.0 0.0 8.23624675844398 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACCGT 35 2.7890076E-4 54.281162 6 TACCTGG 225 0.0 40.111507 2 CGCAGAA 220 0.0 38.86038 9 TCTAGAC 355 0.0 38.8032 3 TAGACCC 395 0.0 37.275356 5 GGGATTC 180 0.0 36.941345 7 GTACAGG 185 0.0 33.391228 1 ATGGGAT 1005 0.0 32.609207 5 CATGGGG 2090 0.0 31.818495 4 ATGGGGT 765 0.0 31.664011 5 GGTGCCG 60 0.0039524483 31.664011 9 ACCCACT 455 0.0 31.316055 8 ACCTGGG 295 0.0 30.593523 3 ATGGGTA 295 0.0 30.590654 5 GCTGGCG 95 3.4926095E-5 29.997486 8 CATTCCG 180 2.1100277E-10 29.025343 9 GTACATG 6505 0.0 28.781252 1 TACAGGG 185 2.8921932E-10 28.243523 2 ACATGGG 6405 0.0 27.9589 3 GCGTGCA 85 6.410018E-4 27.938833 9 >>END_MODULE