##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031606_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96981 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99064765263299 33.0 31.0 34.0 30.0 34.0 2 32.137542405213395 34.0 31.0 34.0 30.0 34.0 3 32.2410987719244 34.0 31.0 34.0 30.0 34.0 4 35.27766263494912 37.0 35.0 37.0 33.0 37.0 5 35.12386962394696 37.0 35.0 37.0 32.0 37.0 6 35.462729813056164 37.0 35.0 37.0 33.0 37.0 7 35.6195337231004 37.0 35.0 37.0 33.0 37.0 8 35.69474433136388 37.0 36.0 37.0 35.0 37.0 9 37.5297738732329 39.0 38.0 39.0 35.0 39.0 10-11 37.5007269465153 39.0 38.0 39.0 35.0 39.0 12-13 37.54309091471525 39.0 38.0 39.0 35.0 39.0 14-15 38.676648003217124 40.5 38.0 41.0 34.5 41.0 16-17 38.70160650024231 40.0 38.5 41.0 35.0 41.0 18-19 38.811808498571885 41.0 39.0 41.0 35.0 41.0 20-21 38.87449603530588 41.0 39.0 41.0 35.0 41.0 22-23 38.792706818861426 41.0 39.0 41.0 35.0 41.0 24-25 38.627225951475026 40.5 38.0 41.0 35.0 41.0 26-27 38.367004877244 40.0 38.0 41.0 34.5 41.0 28-29 38.27186252977387 40.0 38.0 41.0 34.0 41.0 30-31 38.08795537270187 40.0 37.5 41.0 34.0 41.0 32-33 37.72487394438086 40.0 36.5 41.0 34.0 41.0 34-35 37.481114857549414 40.0 35.5 41.0 33.0 41.0 36-37 37.203137728008585 40.0 35.0 41.0 32.5 41.0 38-39 36.91935533764345 39.5 35.0 41.0 31.5 41.0 40-41 36.82521318608799 40.0 35.0 41.0 31.0 41.0 42-43 36.563383549355024 39.0 35.0 41.0 30.5 41.0 44-45 36.10461327476516 39.0 35.0 41.0 29.5 41.0 46-47 35.64578113238676 39.0 35.0 41.0 25.5 41.0 48-49 35.390488858642414 39.0 35.0 41.0 23.0 41.0 50-51 34.90336766995597 38.5 35.0 40.5 22.5 41.0 52-53 35.0757674183603 39.0 35.0 40.5 22.0 41.0 54-55 35.26900114455409 40.0 35.0 41.0 19.5 41.0 56-57 35.02366958476402 39.5 35.0 41.0 16.5 41.0 58-59 34.78292655262371 39.0 35.0 41.0 14.0 41.0 60-61 34.22296635423433 39.0 34.5 41.0 11.5 41.0 62-63 33.94861364597189 38.0 34.0 41.0 10.0 41.0 64-65 33.622271372743114 37.5 34.0 40.5 10.0 41.0 66-67 33.19186747919696 37.0 33.5 39.5 10.0 41.0 68-69 32.78124065538611 37.0 33.0 39.0 10.0 41.0 70-71 32.213464493045024 36.0 32.5 39.0 9.0 41.0 72-73 31.669620853569256 35.0 31.5 37.5 8.0 40.0 74-75 31.24827543539456 35.0 31.0 37.0 8.0 39.0 76-77 30.831740237778533 35.0 31.0 36.5 7.0 39.0 78-79 30.41771068559821 35.0 30.5 36.0 7.0 38.5 80-81 30.036352481413886 35.0 29.0 36.0 7.0 37.0 82-83 29.672920468957837 35.0 29.5 35.0 7.0 37.0 84-85 29.407827306379602 35.0 29.0 35.0 7.0 36.5 86-87 29.397949082810037 35.0 29.0 35.0 7.0 36.0 88-89 29.064466235654407 35.0 28.5 35.0 7.0 36.0 90-91 28.813133500376363 34.0 27.5 35.0 7.0 36.0 92-93 28.603123292191256 34.0 27.0 35.0 7.0 35.5 94-95 28.422309524546044 34.0 25.5 35.0 7.0 35.0 96-97 28.283143089883588 34.0 25.0 35.0 7.0 35.0 98-99 28.260179829038677 34.0 25.0 35.0 7.0 35.0 100-101 27.776162341077118 34.0 24.5 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.01060155746073832 1101 2 0.18081404266669665 1101 3 0.2596740304873606 1101 4 -0.3221347180867511 1101 5 0.1901001737462451 1101 6 -0.09820456407556577 1101 7 0.3274719378048232 1101 8 0.46189863696586286 1101 9 0.45480063441299734 1101 10-11 0.5269746159821977 1101 12-13 0.5102967386071597 1101 14-15 0.7767975773943689 1101 16-17 0.8853401005372277 1101 18-19 1.04911331778208 1101 20-21 0.37153484830742656 1101 22-23 0.46007691653662164 1101 24-25 0.5484751677826054 1101 26-27 0.3696657270571464 1101 28-29 0.7305955986091064 1101 30-31 0.6461485530970066 1101 32-33 0.5441104742948824 1101 34-35 0.37619421829440824 1101 36-37 -0.3608839805366131 1101 38-39 0.21881796913242368 1101 40-41 0.5388797868511688 1101 42-43 0.6874250690386745 1101 44-45 -0.07689989713849599 1101 46-47 0.23107322269279962 1101 48-49 0.029661867183897073 1101 50-51 -0.898140965739735 1101 52-53 -0.008946807127998113 1101 54-55 0.42049612262854197 1101 56-57 0.18009465605927488 1101 58-59 0.2616551993873273 1101 60-61 -0.15546328095506823 1101 62-63 -0.21162239179211184 1101 64-65 0.8686433043423207 1101 66-67 0.22757333904623778 1101 68-69 0.02516297995169481 1101 70-71 -0.33204523372820915 1101 72-73 -0.2495144113793515 1101 74-75 -0.11921448976085713 1101 76-77 0.009017716355543826 1101 78-79 0.8160707107867893 1101 80-81 0.29913261617778275 1101 82-83 -0.12943686294349632 1101 84-85 -0.19563840525299625 1101 86-87 0.1401697097210537 1101 88-89 0.9042504406936906 1101 90-91 0.8182415984787283 1101 92-93 0.7853828166941952 1101 94-95 0.8786564445651166 1101 96-97 0.8321769773500414 1101 98-99 1.3586381192700188 1101 100-101 1.362108579036935 1102 1 -1.3775262964205375 1102 2 -0.7623823043652642 1102 3 -0.18233510193272906 1102 4 -0.3088013847534157 1102 5 -1.2864295066190508 1102 6 -1.5087981713815068 1102 7 -0.6851308019212041 1102 8 -0.5580100388332241 1102 9 -0.505564662390654 1102 10-11 -0.6278199045657473 1102 12-13 -0.29244298742023744 1102 14-15 -0.4778142947517452 1102 16-17 -0.5060754245769274 1102 18-19 -0.5029414767384708 1102 20-21 -0.043350996441439804 1102 22-23 -0.20832490994740027 1102 24-25 -0.217917526281326 1102 26-27 -0.5983708026232151 1102 28-29 -0.8978975520758254 1102 30-31 -0.45595190352400294 1102 32-33 -0.09150596406127676 1102 34-35 -0.8558605762261422 1102 36-37 -0.43330407186081743 1102 38-39 -0.8555199304109564 1102 40-41 -0.480206971139701 1102 42-43 -0.2026205930617877 1102 44-45 -1.4760779793302703 1102 46-47 -1.9224427590423616 1102 48-49 -1.1047673565603944 1102 50-51 -0.38882589724658345 1102 52-53 -0.2362527432010566 1102 54-55 -0.36343081801073396 1102 56-57 -0.18438022978547508 1102 58-59 -0.4969749375989707 1102 60-61 -0.9355546051559855 1102 62-63 -1.2944534420204263 1102 64-65 -1.0420416271645365 1102 66-67 0.037071055941218845 1102 68-69 -0.042051631920450916 1102 70-71 -0.4473876994816308 1102 72-73 -0.06595276754373813 1102 74-75 0.11256633215695189 1102 76-77 -0.14358502337048407 1102 78-79 -0.2599110243730287 1102 80-81 -0.15255688153911606 1102 82-83 0.011567703266546658 1102 84-85 -0.18586671575527802 1102 86-87 -0.003117961511826195 1102 88-89 0.41557464160693414 1102 90-91 0.24518223774813208 1102 92-93 0.31186683495903367 1102 94-95 -0.08527049607415194 1102 96-97 -0.019603844567768647 1102 98-99 -0.08090525972541229 1102 100-101 0.3477706794935571 1103 1 0.4489577218443017 1103 2 0.6054715769132741 1103 3 -0.21977802430716054 1103 4 0.6778652819132489 1103 5 0.9024289408695338 1103 6 0.7237132441436103 1103 7 0.3411705679418091 1103 8 0.7221726095686023 1103 9 0.9068554289335466 1103 10-11 0.8899883146123386 1103 12-13 0.647283039977026 1103 14-15 0.5370715499971155 1103 16-17 0.5154770868386009 1103 18-19 0.8025379753163264 1103 20-21 0.6523567661156378 1103 22-23 0.8230906151667483 1103 24-25 0.9251874965497251 1103 26-27 1.2052821654133083 1103 28-29 0.8812805301159585 1103 30-31 0.392723895562753 1103 32-33 0.009863898952417571 1103 34-35 0.5542764100752251 1103 36-37 0.33774615644968264 1103 38-39 0.7667631746118744 1103 40-41 -0.15975035013513406 1103 42-43 0.3107127402715477 1103 44-45 0.8888535275190463 1103 46-47 0.8749088391311588 1103 48-49 0.40637419595102386 1103 50-51 -0.43923685615069274 1103 52-53 -0.02949475233347698 1103 54-55 -0.6616956581933735 1103 56-57 -0.8746998644886688 1103 58-59 -0.676700964996229 1103 60-61 -0.03217560972219502 1103 62-63 0.02810363560514162 1103 64-65 0.306999468725877 1103 66-67 0.48099799658049136 1103 68-69 0.2306424320064906 1103 70-71 0.20905065668274858 1103 72-73 0.1956910680727013 1103 74-75 0.13421016777338934 1103 76-77 -0.4430370781650055 1103 78-79 -1.0058470974323903 1103 80-81 -1.002237246835918 1103 82-83 -0.8828615204777428 1103 84-85 -0.7572822408694364 1103 86-87 -1.2091453587720977 1103 88-89 -0.24643449081315438 1103 90-91 -0.16121045631579278 1103 92-93 -0.6187267723469034 1103 94-95 -0.3131243773526897 1103 96-97 -0.42809699525269806 1103 98-99 -0.6276632505929953 1103 100-101 -0.15843936616854393 1104 1 0.4192216710591339 1104 2 0.16460956555343387 1104 3 0.5014047354656483 1104 4 0.6412799160595881 1104 5 -0.021226254586530047 1104 6 0.29721808878109357 1104 7 0.40364968588367134 1104 8 0.7397135384025475 1104 9 0.5752483457394248 1104 10-11 0.4660825344586428 1104 12-13 0.7978009150187901 1104 14-15 0.5886084694758935 1104 16-17 0.21335546973201502 1104 18-19 0.07266827935908537 1104 20-21 0.4596571336398583 1104 22-23 0.8462279355810551 1104 24-25 0.48557840867802327 1104 26-27 0.24512513233612765 1104 28-29 0.4486878137778305 1104 30-31 0.8661619176142068 1104 32-33 0.6353233042313988 1104 34-35 0.6619443018226363 1104 36-37 1.222143082610728 1104 38-39 0.778707711865458 1104 40-41 0.8841019719497325 1104 42-43 0.8716883500276467 1104 44-45 0.8807512889624078 1104 46-47 0.3644678100633314 1104 48-49 -0.023629145178283295 1104 50-51 -0.23175272651487688 1104 52-53 -0.2158462391360203 1104 54-55 -0.24505683427089053 1104 56-57 -0.20112819726514886 1104 58-59 0.09351286727908104 1104 60-61 0.22525840297409871 1104 62-63 0.9139205417194134 1104 64-65 0.08990959234766649 1104 66-67 0.5166645518761825 1104 68-69 -0.2697752091562293 1104 70-71 0.3670025444208065 1104 72-73 0.19502284221236366 1104 74-75 0.2189077955715888 1104 76-77 0.1827564400441517 1104 78-79 0.6984629593668075 1104 80-81 0.8058984030137317 1104 82-83 1.420429491884434 1104 84-85 0.10372678017967729 1104 86-87 0.40712594092720167 1104 88-89 0.09720065786709853 1104 90-91 0.42607654669122397 1104 92-93 0.5224359406500909 1104 94-95 0.3960303169339774 1104 96-97 0.4574693261639382 1104 98-99 0.37576140694125115 1104 100-101 0.3427300290870505 1105 1 0.08080703691279467 1105 2 0.11095102896806708 1105 3 0.4468315647339409 1105 4 0.47786528191324606 1105 5 0.007737160047618374 1105 6 0.35453516195183 1105 7 0.16736919807879502 1105 8 0.31532329450011076 1105 9 0.3661020042760086 1105 10-11 0.5159300954342569 1105 12-13 0.32630701257976824 1105 14-15 0.44760237191491825 1105 16-17 0.507257908756408 1105 18-19 0.19080852326153064 1105 20-21 -0.18043432977476925 1105 22-23 0.2825084233859343 1105 24-25 0.37124914038534484 1105 26-27 0.5682958640434492 1105 28-29 0.5183524479241797 1105 30-31 0.6548814298093291 1105 32-33 0.858494035938719 1105 34-35 0.6366394237738575 1105 36-37 0.8796125948058489 1105 38-39 1.0123967362557025 1105 40-41 1.048126362193635 1105 42-43 1.160712740271542 1105 44-45 1.6914220206697266 1105 46-47 2.1429739076243095 1105 48-49 1.1135659767729393 1105 50-51 1.1011741027534256 1105 52-53 1.3545805901322794 1105 54-55 1.9107358486559392 1105 56-57 1.5810364368811918 1105 58-59 1.475525062401033 1105 60-61 1.634862061510681 1105 62-63 1.519713224646246 1105 64-65 1.1992083728354714 1105 66-67 0.5462377226078914 1105 68-69 1.098365034746216 1105 70-71 1.2363623005183655 1105 72-73 0.8027972324562676 1105 74-75 0.922566332156947 1105 76-77 1.4364149766295142 1105 78-79 0.5355056422936393 1105 80-81 0.6482764517942208 1105 82-83 0.7207343699332149 1105 84-85 1.239549950911389 1105 86-87 0.9856320384881769 1105 88-89 1.5414079749402667 1105 90-91 0.8597655710814678 1105 92-93 0.34728350162570365 1105 94-95 1.3876461705925145 1105 96-97 1.271646155432233 1105 98-99 1.138261406941254 1105 100-101 1.205687346160225 1106 1 0.26976052528488736 1106 2 0.3574626568750432 1106 3 0.5564245879897527 1106 4 0.7243769098202222 1106 5 0.7155859972569232 1106 6 0.5975584177657822 1106 7 0.38975291900902675 1106 8 0.4362535270582484 1106 9 0.4062182833457797 1106 10-11 0.3907847465970491 1106 12-13 0.1575570125797654 1106 14-15 0.2869919067986402 1106 16-17 0.40493232736105966 1106 18-19 0.6900817790754843 1106 20-21 0.3830831120856928 1106 22-23 0.47640377222313646 1106 24-25 0.5878189078271987 1106 26-27 0.8008540035783369 1106 28-29 0.6388466339706937 1106 30-31 0.887148871669794 1106 32-33 0.3712847336131375 1106 34-35 0.7555347726110639 1106 36-37 0.2954556180616663 1106 38-39 0.9078909223022222 1106 40-41 1.1661496180075872 1106 42-43 0.4386197170157331 1106 44-45 0.8899685322976367 1106 46-47 0.8753866983219822 1106 48-49 1.2385659767729393 1106 50-51 1.0558252655441223 1106 52-53 0.3275457064113496 1106 54-55 0.86625910446989 1106 56-57 0.6925189950207269 1106 58-59 1.2665134344940512 1106 60-61 0.6550655498827709 1106 62-63 0.5950039223206574 1106 64-65 1.322609535626171 1106 66-67 0.7232726063288126 1106 68-69 0.9406615463741232 1106 70-71 0.9530774167974414 1106 72-73 0.541023976642311 1106 74-75 1.0260547042499724 1106 76-77 0.8329266045364925 1106 78-79 0.8737323864796842 1106 80-81 0.8026369169104974 1106 82-83 1.0012576257471686 1106 84-85 0.8277766950974339 1106 86-87 0.6564169222091074 1106 88-89 1.1137916958704999 1106 90-91 1.2471202222442592 1106 92-93 1.1855102458117486 1106 94-95 1.341861286871584 1106 96-97 0.6229542949671156 1106 98-99 0.00948233717380731 1106 100-101 0.3593210670904554 1107 1 0.6332263917515064 1107 2 0.6926714590755978 1107 3 0.2434713496801777 1107 4 0.7746394754616333 1107 5 0.5406672675744986 1107 6 0.5152878501238689 1107 7 0.666697155068043 1107 8 0.6131727568657013 1107 9 0.7851880257813875 1107 10-11 0.853900525541782 1107 12-13 0.638739808278693 1107 14-15 0.8148738772912623 1107 16-17 0.4545697367134025 1107 18-19 0.5486714264873385 1107 20-21 0.5794447024832934 1107 22-23 0.790169713708508 1107 24-25 0.36251258124555363 1107 26-27 0.6865754339359214 1107 28-29 0.780382017816649 1107 30-31 1.1130803545405143 1107 32-33 1.3375262940032329 1107 34-35 1.4671501764620345 1107 36-37 1.2862658206123 1107 38-39 1.0117918975460256 1107 40-41 1.202158620258146 1107 42-43 1.961250374680148 1107 44-45 2.4082230959385456 1107 46-47 2.009170144183443 1107 48-49 2.2573831810740117 1107 50-51 1.7522493715706204 1107 52-53 1.9106289772290523 1107 54-55 2.0393648809140004 1107 56-57 2.4128240712897977 1107 58-59 2.294207858099959 1107 60-61 2.6179937819407897 1107 62-63 2.4322132246462402 1107 64-65 2.8953777276741732 1107 66-67 2.8633076150810055 1107 68-69 3.0422494433483678 1107 70-71 2.7807843435291204 1107 72-73 2.198563361488521 1107 74-75 2.1585878375332967 1107 76-77 2.703081643296187 1107 78-79 2.429121233691493 1107 80-81 2.452174301256587 1107 82-83 2.1689870581052553 1107 84-85 1.9530580154275157 1107 86-87 2.5276347266602244 1107 88-89 3.092348835155324 1107 90-91 2.739805893662112 1107 92-93 3.0419743618407544 1107 94-95 2.400751009302194 1107 96-97 1.9683531446795435 1107 98-99 2.1042560305971634 1107 100-101 2.0314265934720517 1108 1 0.29276355865192727 1108 2 -0.30861418842323474 1108 3 0.19520112995132877 1108 4 -0.45256950069544644 1108 5 -0.810197622561077 1108 6 -0.09329092500469471 1108 7 -0.1342612367038143 1108 8 0.06314938145663263 1108 9 -0.15672408268051186 1108 10-11 -0.7126025132614018 1108 12-13 -0.5326603787245787 1108 14-15 -0.9341911063459492 1108 16-17 -0.9775246999392451 1108 18-19 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17 787.0 18 884.0 19 1194.0 20 1635.0 21 1945.0 22 1538.0 23 1365.0 24 1348.0 25 1155.0 26 1195.0 27 1263.0 28 1429.0 29 1463.0 30 1533.0 31 1868.0 32 2368.0 33 3105.0 34 4336.0 35 7906.0 36 10871.0 37 17887.0 38 22541.0 39 5849.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.12202550528856 21.357312310290173 14.395673838949952 15.12498834547131 2 14.021998944547345 26.94198114671827 36.18960896513902 22.84641094359537 3 24.76939127560454 30.306066584718362 31.79869640219401 13.125845737483086 4 10.05765534913517 19.31970821020438 43.64551259531731 26.977123845343144 5 8.99441918147995 44.493592393551054 34.19904919388177 12.312939231087226 6 22.60613572977998 40.067141824191715 17.78535275281479 19.54136969321351 7 20.797355918198722 38.52303243131585 24.17269159264615 16.506920057839288 8 23.23527588985064 29.685789245357075 21.79333567459252 25.285599190199765 9 23.768307546117455 20.96717552521225 26.1067156933628 29.157801235307485 10-11 23.77508961499127 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18.151493591528236 36-37 19.66931667027562 27.981254060073624 35.27031067941143 17.079118590239325 38-39 19.454326105113374 31.335519328528267 32.27384745465607 16.93630711170229 40-41 17.5622029057238 32.724451181159196 32.395005207205536 17.318340705911467 42-43 17.228632412534413 33.188459595178436 28.957218424227428 20.625689568059723 44-45 18.18519799858517 30.804025549537084 29.888001321883888 21.122775129993858 46-47 20.694465974267555 32.65643569472434 29.745775848705623 16.903322482302485 48-49 18.912922333904355 31.222781779529836 30.730531877567024 19.13376400899878 50-51 15.410409455074467 32.51394169508313 33.89289270182571 18.182756148016693 52-53 18.305782565626547 34.04686726102031 28.009947168565297 19.63740300478785 54-55 16.272290101543796 30.56014199620243 30.565301741930156 22.60226616032362 56-57 19.19739864254561 29.919739864254563 29.326692301736816 21.556169191463006 58-59 22.183002286109435 29.20182269492566 28.401426367150208 20.213748651814697 60-61 18.98907385988103 30.12588813615334 32.403027924653 18.482010079312623 62-63 17.513257047167176 29.75470586411139 31.74197082932779 20.99006625939364 64-65 16.60694369862448 33.43436325333553 29.875769341980256 20.08292370605973 66-67 18.06146938143532 28.631003070888493 28.665066707955923 24.642460839720265 68-69 16.835526010188335 29.76449153810819 30.743583258925117 22.65639919277836 70-71 20.521712206827154 29.497123100343163 28.374745207317385 21.606419485512294 72-73 19.083244760440422 26.345362048149255 33.26746261879946 21.303930572610856 74-75 16.92884320517917 32.781274038585636 29.476145979070623 20.81373677716457 76-77 18.819110017981522 27.51741314097927 30.331934770477233 23.331542070561976 78-79 19.668652617014466 27.963695313226015 31.617396512024122 20.750255557735397 80-81 20.678532907401003 28.034122753146757 30.618100747789917 20.669243591662323 82-83 18.919964910470096 28.005057020486092 33.21688425615357 19.858093812890242 84-85 18.24595265496545 29.946482667506153 30.955932063436357 20.851632614092036 86-87 18.266715509991382 30.56388242517426 30.424059808995086 20.745342255839272 88-89 18.80441121070911 29.38295687355183 27.805902600384975 24.00672931535409 90-91 19.763078431878597 27.645495135107232 29.327173716674835 23.26425271633933 92-93 21.469442981233023 29.338249723711257 29.744161786426215 19.448145508629505 94-95 19.693762712564403 28.29907178920632 29.204051500727907 22.803113997501367 96-97 17.176799657331003 28.619054254204663 31.67520758826049 22.528938500203846 98-99 20.241308308003532 30.064971591055702 26.393741259282578 23.299978841658195 100-101 19.23936090632851 27.055206178387 28.14478144007594 25.56065147520855 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 521.0 1 322.5 2 197.0 3 377.5 4 301.0 5 137.0 6 268.0 7 552.0 8 704.0 9 605.5 10 396.0 11 236.5 12 190.5 13 176.0 14 173.5 15 167.5 16 155.5 17 143.0 18 144.5 19 144.0 20 150.5 21 158.0 22 197.0 23 245.0 24 275.0 25 336.0 26 439.5 27 576.5 28 723.0 29 928.5 30 1121.5 31 1351.0 32 1857.5 33 2690.0 34 3715.5 35 4160.0 36 4255.0 37 4490.0 38 4702.5 39 4514.0 40 3635.5 41 2956.0 42 2560.5 43 2003.5 44 1573.5 45 1582.0 46 2098.0 47 4996.5 48 11702.5 49 11183.0 50 5014.5 51 2439.0 52 916.0 53 396.0 54 223.5 55 166.5 56 160.5 57 136.0 58 107.5 59 93.0 60 79.0 61 63.0 62 46.0 63 45.5 64 47.0 65 36.5 66 30.5 67 31.5 68 25.5 69 14.0 70 11.0 71 18.0 72 14.0 73 7.0 74 9.0 75 6.5 76 3.0 77 1.5 78 2.5 79 1.5 80 0.5 81 1.0 82 0.5 83 1.0 84 1.0 85 0.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4660706736371042 2 0.3505841350367598 3 0.1773543271362432 4 0.20519483197739766 5 0.22787968777389384 6 0.17632319732731155 7 0.16601189923799506 8 0.17219867809158496 9 0.16807415885585839 10-11 0.18354110598983306 12-13 0.17013641847372166 14-15 0.17941658675410646 16-17 0.07630360586094183 18-19 0.038151802930470914 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.15415390643528115 46-47 0.22272403872923563 48-49 0.08145925490560008 50-51 0.06135222363143296 52-53 0.0711479568162836 54-55 0.07939699528773678 56-57 0.11187758426908365 58-59 0.09434837751724565 60-61 0.15363834153081532 62-63 0.24747115414359513 64-65 0.14951382229508875 66-67 0.10672193522442541 68-69 0.10878419484228868 70-71 0.0912549880904507 72-73 0.07630360586094183 74-75 0.13559356987451152 76-77 0.22169290892030397 78-79 0.13817139439684062 80-81 0.09847289675297224 82-83 0.0886771635681216 84-85 0.09950402656190387 86-87 0.07475691114754437 88-89 0.09434837751724565 90-91 0.11703323331374188 92-93 0.16704302904692672 94-95 0.13198461554325072 96-97 0.09744176694404058 98-99 0.0953795073261773 100-101 0.0644456130582279 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 96981.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.638011569276458 #Duplication Level Percentage of deduplicated Percentage of total 1 76.18645431145431 19.53269197059218 2 8.54649292149292 4.382301687959497 3 3.5472972972972974 2.7283694744331366 4 2.228120978120978 2.2849836565925283 5 1.3835263835263836 1.7735432713624317 6 1.0296010296010296 1.583815386519009 7 0.8405727155727155 1.5085429104669987 8 0.5751287001287002 1.1796125014178034 9 0.5912162162162162 1.3641847372165683 >10 4.2752574002574 22.156917334323218 >50 0.46251608751608747 8.306781740753344 >100 0.24935649935649934 12.040502778894835 >500 0.06435006435006435 11.604334869716748 >1k 0.02010939510939511 9.553417679751703 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3440 3.5470865427248635 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2304 2.3757230797785134 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1451 1.496169352759819 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1050 1.0826862993782287 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1020 1.0517524051102793 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 934 0.9630752415421576 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 931 0.9599818521153628 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 923 0.9517328136439096 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 919 0.9476082944081831 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 820 0.84552644332395 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 774 0.7980944721130944 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 692 0.7135418277806993 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 684 0.7052927893092462 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 624 0.6434250007733473 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 611 0.630020313257236 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 580 0.598055289180355 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 574 0.591868510326765 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 564 0.5815572122374485 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 545 0.5619657458677473 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 543 0.559903486249884 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 536 0.5526855775873625 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 490 0.5052536063765067 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 433 0.44647920726740287 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 427 0.44029242841381305 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 401 0.4134830533815902 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 390 0.40214062548334206 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 358 0.3691444715975294 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 356 0.3670822119796661 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 313 0.3227436301956053 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 307 0.31655685134201544 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 297 0.306245553252699 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 265 0.2732493993668863 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 254 0.2619069714686382 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 251 0.25881358204184324 No Hit ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT 237 0.24437776471680023 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 236 0.24334663490786856 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 224 0.2309730772006888 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 216 0.22272403872923563 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 205 0.2113816108309875 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 195 0.20107031274167103 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 193 0.19900805312380773 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 192 0.19797692331487612 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 180 0.18560336560769636 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 168 0.1732298079005166 No Hit TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC 163 0.16807415885585839 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 163 0.16807415885585839 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 162 0.16704302904692672 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 161 0.16601189923799506 No Hit ACTTACTACTCCGGAAAAAAAGAACCTTTCGGCTATATAGGAATAGTATG 155 0.1598251203844052 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 155 0.1598251203844052 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 152 0.15673173095761025 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 152 0.15673173095761025 No Hit TCAGTAGACCTAACAATTTTCTCCCTTCATTTAGCTGGAGTGTCATCTAT 149 0.15363834153081532 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 147 0.15157608191295202 No Hit GTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTCAG 146 0.15054495210402036 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 144 0.1484826924861571 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 144 0.1484826924861571 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 139 0.14332704344149885 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 135 0.13920252420577228 No Hit TAGTAATACCAATAATAATTGGAGGCTTTGGAAACTGACTTGTCCCACTA 132 0.13610913477897732 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 128 0.13198461554325072 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 127 0.1309534857343191 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 127 0.1309534857343191 No Hit GTGTAGGGTTGCAAGTCAGCTAAATACTTTGACACCGGTAGGAATTGCGA 127 0.1309534857343191 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 125 0.1288912261164558 No Hit TAATTATAGTGGCTGATGTAAAGTAAGCTCGTGTGTCTACATCTAATCCT 123 0.12682896649859252 No Hit GTTTATATTCTTATCCTCCCAGGATTTGGAATTATTTCACATGTAGTTAC 122 0.12579783668966088 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 121 0.12476670688072922 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 121 0.12476670688072922 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 121 0.12476670688072922 No Hit TTCTTATCCTCCCAGGATTTGGAATTATTTCACATGTAGTTACTTACTAC 121 0.12476670688072922 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 119 0.12270444726286592 No Hit GTCTACATCTAATCCTACTGTGAATATGTGGTGGGCTCATACAATAAAGC 118 0.12167331745393428 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 118 0.12167331745393428 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 115 0.11857992802713933 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 113 0.11651766840927605 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 111 0.11445540879141275 No Hit GTACAAAGCAGTGGTATCAACGCAGAGTACAAAGCAGTGGTATCAACGCA 110 0.1134242789824811 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 110 0.1134242789824811 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 107 0.11033088955568617 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 103 0.10620637031995958 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 102 0.10517524051102793 No Hit ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT 101 0.10414411070209628 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 100 0.10311298089316465 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 100 0.10311298089316465 No Hit CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT 99 0.102081851084233 No Hit ATGTATAAGTTGATCGTAACGGAAGCGTGGATAAGATGCTCGGATCCATA 99 0.102081851084233 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 99 0.102081851084233 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 99 0.102081851084233 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 98 0.10105072127530135 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 98 0.10105072127530135 No Hit CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA 98 0.10105072127530135 No Hit CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG 97 0.1000195914663697 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 97 0.1000195914663697 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.002062259617863293 0.0 12-13 0.0 0.0 0.0 0.009795733184850641 0.0 14-15 0.0 0.0 0.0 0.016498076942906344 0.0 16-17 0.0 0.0 0.0 0.03299615388581269 0.0 18-19 0.0 0.0 0.0 0.045885276497458265 0.0 20-21 0.0 0.0 0.0 0.0819748198100659 0.0 22-23 0.0 0.0 0.0 0.16343407471566596 0.0 24-25 0.0 0.0 0.0 0.24798671904806097 0.0 26-27 0.0 0.0 0.0 0.29748094987678 0.0 28-29 0.0 0.0 0.0 0.3402728369474433 0.0 30-31 0.0 0.0 0.0 0.4176075726173168 0.0 32-33 0.0 0.0 0.0 0.49545787319165613 0.0 34-35 0.0 0.0 0.0 0.5975397242758891 0.0 36-37 0.0 0.0 0.0 0.7166352172074942 0.0 38-39 0.0 0.0 0.0 0.8728513832606386 0.0 40-41 0.0 0.0 0.0 1.1301182705890844 0.0 42-43 0.0 0.0 0.0 1.3100504222476568 0.0 44-45 0.0 0.0 0.0 1.4600798094472114 0.0 46-47 0.0 0.0 0.0 1.6369185716789887 0.0 48-49 0.0 0.0 0.0 1.7910724781142697 0.0 50-51 0.0 0.0 0.0 1.959662201874594 0.0 52-53 0.0 0.0 0.0 2.1179406275456016 0.0 54-55 0.0 0.0 0.0 2.2865303513059256 0.0 56-57 0.0 0.0 0.0 2.475227106340417 0.0 58-59 0.0 0.0 0.0 2.6608304719481137 0.0 60-61 0.0 0.0 0.0 2.8593229601674555 0.0 62-63 0.0 0.0 0.0 3.0433796310617542 0.0 64-65 0.0 0.0 0.0 3.1954712778791725 0.0 66-67 0.0 0.0 0.0 3.388808117053856 0.0 68-69 0.0 0.0 0.0 3.6378259659108485 0.0 70-71 0.0 0.0 0.0 3.9084975407554055 0.0 72-73 0.0 0.0 0.0 4.1745290314597705 0.0 74-75 0.0 0.0 0.0 4.433342613501614 0.0 76-77 0.0 0.0 0.0 4.673595858982687 0.0 78-79 0.0 0.0 0.0 5.082954393128551 0.0 80-81 0.0 0.0 0.0 5.479939369567235 0.0 82-83 0.0 0.0 0.0 5.829492374795063 0.0 84-85 0.0 0.0 0.0 6.264113589259752 0.0 86-87 0.0 0.0 0.0 6.682236726781534 0.0 88-89 0.0 0.0 0.0 7.157072003794558 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACATAT 20 0.0020600425 71.32209 1 ACATATA 25 0.004997638 57.028202 2 AATCAAG 40 5.3505495E-4 47.523502 6 TGGGGCA 55 4.9151888E-5 43.203186 8 CGCAGAA 135 0.0 42.243114 9 TAAATCA 50 0.0016046104 38.018803 4 ATAAATC 50 0.0016046104 38.018803 3 ACATAAA 65 1.3113869E-4 36.575436 1 GGATATG 55 0.0025617986 34.56255 9 GTATAAG 55 0.0025617986 34.56255 3 GGGGCAG 70 2.0336037E-4 33.94536 9 TGATCAC 35 2.0597756E-4 33.892838 26-27 GGAAGCG 35 2.0597756E-4 33.892838 20-21 TAGGATA 60 0.003923744 31.682335 7 ATCCAAC 60 0.003923744 31.682335 6 TGATCGT 30 0.0039333752 31.682335 10-11 TATCCAA 60 0.003923744 31.682335 5 ATGGGGC 75 3.0489598E-4 31.682333 7 CGGATCC 30 0.003938353 31.674154 40-41 CGTAACG 30 0.003938353 31.674154 14-15 >>END_MODULE