FastQCFastQC Report
Fri 10 Feb 2017
SRR2031605_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031605_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052412
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16550.1572578039779098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14220.13511818565352732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15000.047.0103231
GTATCAA25250.037.9277841
TCAACGC28250.033.4939654
CAACGCA29000.033.1196175
AACGCAG29550.032.643866
ATCAACG29000.032.6277433
TATCAAC31400.030.3171122
ACGCAGA33950.028.1318667
CTGTCGA856.392214E-427.9520769
CGCAGAG34200.027.7886128
GTACATG31100.026.5038221
GTATAGG4500.024.3521961
TACATGG32550.023.8354912
GCAGAGT40000.023.6404679
ACATGGG32050.023.1434733
ATAGCCC3400.022.3755133
GAGTACT22600.021.75973112-13
GTGTAGC5950.021.6206931
CATGGGG18000.021.1324314
AGAGTAC36500.020.82855210-11
Produced by FastQC (version 0.11.5)