##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031605_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1052412 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00861829777692 33.0 31.0 34.0 30.0 34.0 2 32.25084662660631 34.0 31.0 34.0 30.0 34.0 3 32.389927138801156 34.0 31.0 34.0 31.0 34.0 4 35.482585717380644 37.0 35.0 37.0 33.0 37.0 5 35.21331569765453 37.0 35.0 37.0 32.0 37.0 6 35.42603752142697 37.0 35.0 37.0 33.0 37.0 7 35.577874444609144 37.0 35.0 37.0 33.0 37.0 8 35.65409649452876 37.0 36.0 37.0 33.0 37.0 9 37.58525938510773 39.0 38.0 39.0 35.0 39.0 10-11 37.54480089546679 39.0 38.0 39.0 35.0 39.0 12-13 37.56887844304322 39.0 38.0 39.0 35.0 39.0 14-15 39.00640338574627 41.0 39.0 41.0 36.0 41.0 16-17 38.9785288461173 41.0 39.0 41.0 36.0 41.0 18-19 38.92962309437749 41.0 39.0 41.0 35.0 41.0 20-21 38.94781368893551 41.0 39.0 41.0 35.0 41.0 22-23 38.89694340239374 41.0 39.0 41.0 35.0 41.0 24-25 38.83342645275805 41.0 39.0 41.0 35.0 41.0 26-27 38.74624434156965 41.0 39.0 41.0 34.5 41.0 28-29 38.65272583360889 40.5 38.5 41.0 34.0 41.0 30-31 38.52637512685146 40.0 38.0 41.0 34.0 41.0 32-33 38.381110249598066 40.0 38.0 41.0 34.0 41.0 34-35 38.26524117931 40.0 38.0 41.0 34.0 41.0 36-37 38.13845623196999 40.0 38.0 41.0 33.5 41.0 38-39 37.98234911802602 40.0 38.0 41.0 33.0 41.0 40-41 37.85071435901529 40.0 38.0 41.0 33.0 41.0 42-43 37.69441340463621 40.0 38.0 41.0 32.5 41.0 44-45 37.49940517591969 40.0 37.5 41.0 31.5 41.0 46-47 37.306384286762224 40.0 37.0 41.0 31.0 41.0 48-49 37.417705233311665 40.0 37.5 41.0 31.0 41.0 50-51 37.203112469261086 39.5 37.5 40.5 31.0 41.0 52-53 37.34544028384321 40.0 37.5 40.5 31.5 41.0 54-55 37.63547213448726 40.0 38.0 41.0 32.0 41.0 56-57 37.56504296796312 40.0 37.0 41.0 32.0 41.0 58-59 37.40958721489302 40.0 37.0 41.0 31.0 41.0 60-61 37.184206850549025 40.0 36.5 41.0 31.0 41.0 62-63 36.915896055917266 40.0 36.0 41.0 30.5 41.0 64-65 36.6969086251392 39.0 35.5 41.0 30.0 41.0 66-67 36.52315775570784 39.0 35.0 41.0 30.0 41.0 68-69 36.317396608932626 39.0 35.0 41.0 30.0 41.0 70-71 36.049001246660055 38.0 35.0 41.0 29.5 41.0 72-73 35.71575248096753 37.0 35.0 40.0 29.0 41.0 74-75 35.310318582456304 37.0 35.0 39.5 29.0 41.0 76-77 34.90689055236922 36.5 34.5 39.0 29.0 41.0 78-79 34.532931019410654 36.0 34.0 39.0 28.0 40.5 80-81 34.1571147991471 35.5 34.0 37.5 28.0 39.0 82-83 33.77176096433716 35.0 34.0 37.0 27.0 39.0 84-85 33.45788578997579 35.0 34.0 37.0 27.0 39.0 86-87 33.16538722477509 35.0 34.0 36.0 26.5 37.0 88-89 32.87899178268587 35.0 34.0 36.0 26.0 37.0 90-91 32.65802033804252 35.0 34.0 35.5 26.0 37.0 92-93 32.45538914417547 35.0 33.0 35.0 25.5 36.0 94-95 32.290911259088645 35.0 33.0 35.0 25.0 36.0 96-97 32.174051607165254 35.0 33.0 35.0 25.0 36.0 98-99 32.06629722960209 35.0 33.0 35.0 25.0 36.0 100-101 31.63009543790835 34.5 32.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15403164207238973 1101 2 0.15325496153103302 1101 3 0.1711887611896401 1101 4 0.2930472479274471 1101 5 0.28151430945688816 1101 6 0.28199485180281414 1101 7 0.3192581944459505 1101 8 0.2770293239510977 1101 9 0.1900023482472193 1101 10-11 0.3510449997928262 1101 12-13 0.2799604985535282 1101 14-15 0.37510789095176733 1101 16-17 0.34921358319689944 1101 18-19 0.43735635512736337 1101 20-21 0.1669727211482126 1101 22-23 0.2296122113516006 1101 24-25 0.30950849332273833 1101 26-27 0.30711410539582573 1101 28-29 0.38947758571406155 1101 30-31 0.37519416685639584 1101 32-33 0.2017546410428679 1101 34-35 0.3223937470694622 1101 36-37 0.31210562231633077 1101 38-39 0.19725653263051157 1101 40-41 0.15653091345038206 1101 42-43 -0.004676475534736824 1101 44-45 0.21777794378001403 1101 46-47 0.4251954621358962 1101 48-49 0.36721805935307117 1101 50-51 0.2838443475012653 1101 52-53 0.2790369218746207 1101 54-55 0.24354318925904295 1101 56-57 0.35911532357626186 1101 58-59 0.5561742476891993 1101 60-61 0.30434367045943844 1101 62-63 0.12488008244363868 1101 64-65 0.34495310387278977 1101 66-67 0.054032099061409156 1101 68-69 0.13565430178710614 1101 70-71 0.21514774695902616 1101 72-73 0.2394502144099988 1101 74-75 0.2033719979380848 1101 76-77 0.12052325924450713 1101 78-79 0.14711388250015744 1101 80-81 0.205603916270654 1101 82-83 0.1770220152715325 1101 84-85 0.0012201653532173395 1101 86-87 0.13847926350675266 1101 88-89 0.19566547498828157 1101 90-91 0.23361358111193908 1101 92-93 0.30608381359852643 1101 94-95 0.09407573619556331 1101 96-97 0.30984837874343185 1101 98-99 0.19762510868259398 1101 100-101 0.2566903551707078 1102 1 0.09534689878643121 1102 2 0.22090275159328598 1102 3 0.1819352842987243 1102 4 0.18846906671813457 1102 5 0.35135909881387306 1102 6 0.151973592156061 1102 7 0.06135432458137302 1102 8 0.32669774449634303 1102 9 0.3376630260189657 1102 10-11 0.4463557001434282 1102 12-13 0.37856796632156886 1102 14-15 0.3000598157362546 1102 16-17 0.3964998070472845 1102 18-19 0.37721793909789625 1102 20-21 0.3906620055759049 1102 22-23 0.28354017209990445 1102 24-25 0.5106201851136731 1102 26-27 0.36403313100380785 1102 28-29 0.3673769398064124 1102 30-31 0.47345787780638204 1102 32-33 0.4437683202809737 1102 34-35 0.32515648636733374 1102 36-37 0.2893567855082182 1102 38-39 0.19394073808292944 1102 40-41 0.3682901872736224 1102 42-43 0.26095748307237443 1102 44-45 0.049881278855387734 1102 46-47 -0.4183685476668799 1102 48-49 0.36379317592595584 1102 50-51 0.37275305279384696 1102 52-53 0.4580073765469237 1102 54-55 0.32056754905953255 1102 56-57 0.31074962720866495 1102 58-59 0.2324986627952228 1102 60-61 -0.03508384124859276 1102 62-63 -0.18571117928341607 1102 64-65 0.248451305174747 1102 66-67 0.19035768116474117 1102 68-69 0.35792669925624665 1102 70-71 0.2426685880089181 1102 72-73 0.2968753616799944 1102 74-75 0.2920637939469586 1102 76-77 0.4323804593642535 1102 78-79 0.3046153919858199 1102 80-81 0.3665265037574059 1102 82-83 0.33256246187633565 1102 84-85 0.29766919547697057 1102 86-87 0.43213637939972926 1102 88-89 0.33572925392854813 1102 90-91 0.2673727839005551 1102 92-93 0.3896091095338221 1102 94-95 0.49055419494727914 1102 96-97 0.2209041561412377 1102 98-99 0.3255001080483666 1102 100-101 0.5108319271668371 1103 1 0.3207825443244019 1103 2 0.25474910609961654 1103 3 0.2425269894994031 1103 4 0.3867963748827492 1103 5 0.2953877833747569 1103 6 0.5200027098400355 1103 7 0.45054125902967 1103 8 0.3988631876723119 1103 9 0.507432511020852 1103 10-11 0.46969082175011323 1103 12-13 0.28919572409747474 1103 14-15 0.5346242686353477 1103 16-17 0.5290379329532087 1103 18-19 0.50245818706086 1103 20-21 0.08528684961467548 1103 22-23 0.25158627017767543 1103 24-25 0.36563161611790207 1103 26-27 0.3192516489845616 1103 28-29 0.48010153012365464 1103 30-31 0.4374311422993955 1103 32-33 0.5329541473122816 1103 34-35 0.54893900443669 1103 36-37 0.5429195920473902 1103 38-39 0.46831437073056037 1103 40-41 0.4190640256547269 1103 42-43 0.2697903640053667 1103 44-45 0.4295087437810281 1103 46-47 0.7474105447925936 1103 48-49 0.46177927661231166 1103 50-51 0.5404553594203847 1103 52-53 0.47612533368249643 1103 54-55 0.3760091500399625 1103 56-57 0.385538418180019 1103 58-59 0.5184663360629145 1103 60-61 0.5882774892267832 1103 62-63 0.5714814940457984 1103 64-65 0.5613513595612218 1103 66-67 0.4430868488055353 1103 68-69 0.3814217810943603 1103 70-71 0.47661288739541163 1103 72-73 0.5205280658692288 1103 74-75 0.46474824655073377 1103 76-77 -0.20653515792228916 1103 78-79 0.16625102226604582 1103 80-81 0.2960830939958967 1103 82-83 0.17064148715250838 1103 84-85 0.11316798839245479 1103 86-87 0.2707420429763019 1103 88-89 0.42143102407307254 1103 90-91 0.33834529288909465 1103 92-93 0.18243846149801612 1103 94-95 0.09852858162284406 1103 96-97 0.33511206998911547 1103 98-99 0.44858704520368065 1103 100-101 0.4205306851371624 1104 1 0.14572889631860875 1104 2 0.3483574047517024 1104 3 0.2530116440015533 1104 4 0.046778771539287334 1104 5 0.09724820597219974 1104 6 0.15541543323666218 1104 7 0.01638678793278814 1104 8 0.21376438275416376 1104 9 0.2813760467144988 1104 10-11 0.27410384634909235 1104 12-13 0.21394025879845202 1104 14-15 0.28114139673541416 1104 16-17 0.23202428977602807 1104 18-19 0.3747196775788453 1104 20-21 0.21635968081127288 1104 22-23 0.3770681060352885 1104 24-25 0.2921001533644443 1104 26-27 0.26758665631538747 1104 28-29 0.2856256046296295 1104 30-31 0.3195022190216079 1104 32-33 0.19979856633748483 1104 34-35 0.12002217261660064 1104 36-37 0.22631138832678488 1104 38-39 0.19185104885541904 1104 40-41 0.06615156302667202 1104 42-43 0.03273239603126399 1104 44-45 0.25315945688051755 1104 46-47 0.38890357084483895 1104 48-49 0.18496904463962238 1104 50-51 0.22228840424796914 1104 52-53 0.16963084849825094 1104 54-55 0.1079173782552374 1104 56-57 0.07494019750065917 1104 58-59 0.14954268324022735 1104 60-61 0.10991600656115708 1104 62-63 0.16130366746099867 1104 64-65 0.35348540749810553 1104 66-67 0.33671026617470545 1104 68-69 0.2010015745187772 1104 70-71 0.21703857890091882 1104 72-73 0.18758402973432453 1104 74-75 -0.028940568059802274 1104 76-77 0.2658901772971731 1104 78-79 0.3943549593440494 1104 80-81 0.351878192015576 1104 82-83 0.22124875562225554 1104 84-85 0.1399093657427386 1104 86-87 0.26131628466715284 1104 88-89 0.17387257968358938 1104 90-91 0.22029741226366895 1104 92-93 0.0366354290818478 1104 94-95 0.15494542223288477 1104 96-97 0.13741960813457155 1104 98-99 0.2203682527555344 1104 100-101 0.09453188291459114 1105 1 -0.08356411177859968 1105 2 0.047917986555020775 1105 3 0.004001406293781429 1105 4 0.03354567990977131 1105 5 -0.20495811510086526 1105 6 -0.05308350145715934 1105 7 0.03243835336698453 1105 8 0.11728796947909359 1105 9 0.0881562068565529 1105 10-11 0.21797720951905575 1105 12-13 0.1542589386319193 1105 14-15 0.15259701483825694 1105 16-17 0.11336324613858295 1105 18-19 0.16530707389142663 1105 20-21 0.12732997200961194 1105 22-23 0.1572524221350875 1105 24-25 0.21972251523450126 1105 26-27 0.13306414313778703 1105 28-29 0.19378016907378282 1105 30-31 0.07350494629333326 1105 32-33 0.2340603294823964 1105 34-35 0.1950265404268805 1105 36-37 0.05767600214092994 1105 38-39 0.10504590154209126 1105 40-41 0.1860982262943196 1105 42-43 0.2114807738979465 1105 44-45 0.17619094796853574 1105 46-47 0.2916722680946009 1105 48-49 0.1542789965442637 1105 50-51 0.15122660330364823 1105 52-53 0.005736322051298259 1105 54-55 0.14291919680266574 1105 56-57 0.13416550728555166 1105 58-59 0.08499029314147322 1105 60-61 0.1352960060556967 1105 62-63 0.18826871508935739 1105 64-65 0.08148565363382687 1105 66-67 -0.04151518316034242 1105 68-69 -0.037468779861733026 1105 70-71 0.005681063465239333 1105 72-73 0.04229252921842175 1105 74-75 0.06497449533662802 1105 76-77 0.07141823182502804 1105 78-79 0.1467709902516674 1105 80-81 0.010336305539084378 1105 82-83 0.07559794090201422 1105 84-85 0.18934418757453386 1105 86-87 0.06955732626079936 1105 88-89 0.1517330009492781 1105 90-91 0.15985186265029228 1105 92-93 0.2241403050964692 1105 94-95 0.13591275760489907 1105 96-97 0.1718110320626991 1105 98-99 -0.03316637711237291 1105 100-101 0.08973278570526944 1106 1 0.11996736584887202 1106 2 -0.010549755380459658 1106 3 0.023284704941026746 1106 4 0.082257958785938 1106 5 0.01941753526333656 1106 6 -0.10960015078077845 1106 7 0.09545864473015087 1106 8 0.1151092493958501 1106 9 0.15660703932273634 1106 10-11 0.18955629380625538 1106 12-13 0.2013453069981992 1106 14-15 0.08349581816812446 1106 16-17 0.02853949483785101 1106 18-19 0.00853678252826029 1106 20-21 -0.1744877959404434 1106 22-23 0.09265499757733409 1106 24-25 -0.07746140776237098 1106 26-27 -0.01063382772589705 1106 28-29 -0.04072490376700699 1106 30-31 -0.004961494913736431 1106 32-33 0.05155187474775147 1106 34-35 -0.03121890702369967 1106 36-37 0.08884470661543986 1106 38-39 0.17583804514250545 1106 40-41 0.06813841880212834 1106 42-43 -0.01601662464523912 1106 44-45 0.28930554214127113 1106 46-47 0.19551592053997524 1106 48-49 0.08537290913531592 1106 50-51 -0.07984779836128553 1106 52-53 -0.02252200261050774 1106 54-55 0.0970197431086035 1106 56-57 0.1977223491169724 1106 58-59 0.19407262924969615 1106 60-61 0.28309127140429524 1106 62-63 0.18399257044836048 1106 64-65 0.4368934319897093 1106 66-67 0.25204295939948906 1106 68-69 0.25488618892078563 1106 70-71 0.08284677626441095 1106 72-73 0.1154259839530738 1106 74-75 0.0375453850348606 1106 76-77 0.0036177635628007465 1106 78-79 0.20327138047019133 1106 80-81 0.17374031178258065 1106 82-83 0.23495342997590285 1106 84-85 0.13154306894810475 1106 86-87 0.13926010981958825 1106 88-89 0.07307145048100949 1106 90-91 0.23948895942448445 1106 92-93 0.38655250592893253 1106 94-95 0.22579309059136676 1106 96-97 0.13761813403980483 1106 98-99 0.3026979569146775 1106 100-101 0.4574324475158811 1107 1 0.23564276907467985 1107 2 0.14330609139373252 1107 3 0.11419952348941109 1107 4 0.3022050353988419 1107 5 0.35475649695688816 1107 6 0.22961355889663793 1107 7 0.2066607616656313 1107 8 0.25875844294423445 1107 9 0.32555235182273634 1107 10-11 0.3530517575159351 1107 12-13 0.419559772724007 1107 14-15 0.38619869115199634 1107 16-17 0.2535741470555948 1107 18-19 0.3790162432137478 1107 20-21 0.3202986203902043 1107 22-23 0.28250189777894974 1107 24-25 0.3836021003021415 1107 26-27 0.4387109303386154 1107 28-29 0.42117340772492895 1107 30-31 0.3339371946023917 1107 32-33 0.476010040070328 1107 34-35 0.37484208087953164 1107 36-37 0.49040090621220855 1107 38-39 0.5501922538118649 1107 40-41 0.38717508714889703 1107 42-43 0.18485598120153668 1107 44-45 0.42789866210095084 1107 46-47 0.42609492759642364 1107 48-49 0.330757863772412 1107 50-51 0.3344982197838746 1107 52-53 0.47161862238949226 1107 54-55 0.4599702219392441 1107 56-57 0.4905020870201966 1107 58-59 0.344668017354536 1107 60-61 0.4901855254365515 1107 62-63 0.2765612448435206 1107 64-65 0.5457171467074531 1107 66-67 0.4008112305688485 1107 68-69 0.3807524943643372 1107 70-71 0.45543687203053906 1107 72-73 0.4430154496788816 1107 74-75 0.5798368416880848 1107 76-77 0.5855860095305445 1107 78-79 0.46895938450244756 1107 80-81 0.42295276137935645 1107 82-83 0.5436731880404153 1107 84-85 0.5972216223755282 1107 86-87 0.41044521566636405 1107 88-89 0.4841570099568173 1107 90-91 0.5652828102309329 1107 92-93 0.4932340835499005 1107 94-95 0.5518232065187902 1107 96-97 0.3474058104511002 1107 98-99 0.3631345748582291 1107 100-101 0.5617041209029772 1108 1 0.22712205377722938 1108 2 0.2768009863417973 1108 3 0.10438963503332133 1108 4 0.11501672390214424 1108 5 0.24043782220476118 1108 6 0.31505678363696177 1108 7 0.2717261978082206 1108 8 0.16682324464395037 1108 9 0.21388395150403738 1108 10-11 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-1.1016619748367376 2316 80-81 -1.0997901781259927 2316 82-83 -0.9924529101261257 2316 84-85 -1.367048798150634 2316 86-87 -0.9105428208834851 2316 88-89 -0.8047894812817233 2316 90-91 -1.01270044272934 2316 92-93 -1.006168713790629 2316 94-95 -1.1526569697846867 2316 96-97 -1.0635431039157837 2316 98-99 -0.6484047532309631 2316 100-101 -1.1678559559686796 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 6.0 7 74.0 8 329.0 9 313.0 10 308.0 11 410.0 12 563.0 13 780.0 14 986.0 15 1346.0 16 1714.0 17 2175.0 18 2811.0 19 3624.0 20 4870.0 21 5689.0 22 6646.0 23 7013.0 24 6892.0 25 7111.0 26 7694.0 27 8563.0 28 10131.0 29 11878.0 30 14358.0 31 17637.0 32 21978.0 33 28529.0 34 40013.0 35 58921.0 36 99742.0 37 190275.0 38 324344.0 39 164551.0 40 138.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.09989162453391 22.270919568216865 15.824250323455411 20.80493848379381 2 13.567249677147375 21.078127473852277 43.87457485507398 21.480047993926366 3 17.102815128530814 28.080064441554136 31.790595417870342 23.026525012044704 4 11.40270088280142 18.549782704559156 42.497997529413226 27.549518883226202 5 11.72223460664224 39.586798589319294 36.84574538013501 11.845221423903455 6 27.159462902694436 40.70591618280809 19.71096075365774 12.423660160839734 7 24.674914170486073 34.3408502786722 24.59160765391712 16.39262789692461 8 23.76556051164628 38.1557312666911 21.457630184655372 16.62107803700724 9 24.513654241083543 18.02771121158718 22.664907224661132 34.79372732266815 10-11 22.90166575574121 28.53080419800155 30.174516812763873 18.393013233493363 12-13 23.450839595668775 25.826813935088786 30.400675218289162 20.321671250953273 14-15 21.02138801937657 27.666075530534762 27.82280198526524 23.489734464823435 16-17 20.280715236096196 30.942301967650604 28.511246774713566 20.265736021539634 18-19 20.168041266022403 30.042343015018485 30.372617581595495 19.41699813736361 20-21 21.133961353391463 29.009519121861484 30.269914329803647 19.586605194943406 22-23 20.74344458253992 28.793666358802444 30.057952588910048 20.40493646974759 24-25 20.504564752207312 28.766965789063597 30.247659661805454 20.480809796923637 26-27 20.5434278590514 29.01549013124138 30.005549157554267 20.43553285215296 28-29 20.66543330938834 28.895147527774295 29.963740436255005 20.475678726582366 30-31 20.90193764419258 28.66957047240054 29.92896318171971 20.499528701687172 32-33 20.62001383488596 28.917144616366976 29.96792130838493 20.494920240362138 34-35 20.722207652516314 28.934295694081786 29.898651858777743 20.44484479462416 36-37 21.023040406228738 28.766205630494525 29.649652417494288 20.56110154578245 38-39 20.743302052808215 28.906312356757617 29.82909734970715 20.521288240727014 40-41 20.851434609259492 28.988932091234233 29.812183821545172 20.347449477961103 42-43 20.883551308803018 28.885645545660825 29.63853509842153 20.59226804711463 44-45 20.979043936082245 29.125341488781658 29.166353446955174 20.729261128180926 46-47 21.061744922987856 28.890170097045964 29.187868492816772 20.86021648714941 48-49 21.187576708777932 28.78662584091366 29.266815063428442 20.758982386879964 50-51 20.90247110844039 29.07131029741822 29.020061622015824 21.00615697212557 52-53 21.004183463835787 29.10918702731589 28.977868387533572 20.908761121314747 54-55 20.98691173911348 29.042425821618124 29.15630500792637 20.814357431342025 56-57 21.132768280966108 29.127355573946712 28.94761374770507 20.792262397382114 58-59 21.084790834564597 28.922556312673663 29.002067187640435 20.99058566512131 60-61 21.12515837065322 28.915543422461777 29.06631371162768 20.892984495257323 62-63 20.946399010959077 28.833771785283634 29.35148479959158 20.868344404165708 64-65 21.003668837903206 28.992430167438304 29.05903251526621 20.94486847939228 66-67 20.79221576622187 28.98888951773725 29.01947057203693 21.19942414400395 68-69 21.017305784353546 28.8135649649423 29.055939166738803 21.11319008396535 70-71 21.12802506215132 28.600496830140017 29.14564046768987 21.125837640018794 72-73 20.902962249070836 28.92770320739547 29.038437491233733 21.13089705229996 74-75 20.792286941752423 29.08263515217257 28.958175737074725 21.16690216900028 76-77 20.872999435806975 28.944959042443024 29.08750705241282 21.09453446933718 78-79 20.937020378530466 28.956648166103534 28.75706854406309 21.349262911302915 80-81 20.89788574170224 29.120682907855276 28.68682795635012 21.294603394092366 82-83 21.040863626550564 28.992232801032458 28.701973326942298 21.26493024547468 84-85 21.124385382628592 28.908500421594585 28.757601424052815 21.20951277172401 86-87 20.776549657110905 28.967139319851054 29.0459676775207 21.210343345517337 88-89 20.877692648262396 28.855164686854586 28.828961641309313 21.438181023573705 90-91 20.964573483385497 28.91817844538095 28.807066277266717 21.310181793966837 92-93 21.038127325852535 28.71731909507086 28.8441881049596 21.40036547411701 94-95 20.78496780282848 28.654911781272357 28.994532950722345 21.56558746517682 96-97 20.77909417938092 28.67008566517115 29.164913181689574 21.385906973758352 98-99 21.0604894252033 28.507898349199134 28.7870020412132 21.644610184384362 100-101 20.94115689513781 28.41640618500339 28.90540652967114 21.737030390187666 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 16.0 1 13.0 2 24.5 3 55.0 4 61.5 5 53.0 6 71.0 7 142.0 8 267.0 9 341.0 10 331.5 11 332.5 12 390.5 13 453.5 14 502.0 15 538.5 16 581.0 17 679.0 18 757.5 19 830.0 20 931.0 21 1054.5 22 1266.0 23 1573.0 24 1982.0 25 2467.0 26 3169.5 27 4390.0 28 6465.5 29 9754.0 30 14745.5 31 21583.0 32 29728.5 33 38074.0 34 45972.0 35 52777.5 36 58890.5 37 61940.0 38 60817.0 39 56707.0 40 50488.0 41 43753.5 42 37630.5 43 33157.5 44 30238.0 45 28509.0 46 27026.0 47 26305.0 48 26064.5 49 25729.0 50 25345.5 51 24601.5 52 23566.5 53 22527.5 54 21310.0 55 19706.0 56 17858.5 57 15965.0 58 14181.0 59 12410.0 60 10594.5 61 8733.0 62 7028.5 63 5172.5 64 3553.0 65 2645.5 66 1919.5 67 1404.0 68 1047.0 69 759.0 70 606.5 71 472.5 72 341.5 73 269.0 74 202.5 75 147.5 76 101.5 77 72.5 78 53.5 79 39.5 80 35.0 81 30.0 82 23.5 83 17.5 84 10.5 85 7.5 86 7.0 87 6.0 88 5.5 89 3.5 90 2.5 91 2.0 92 0.5 93 1.0 94 2.0 95 1.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4871666229575489 2 0.3749482141974816 3 0.20505277400865823 4 0.23365374017019952 5 0.2571236359904676 6 0.20657309114681324 7 0.19707110903334435 8 0.19735616849674842 9 0.1975462081390178 10-11 0.20885356685404577 12-13 0.19811632706582594 14-15 0.2066206010573806 16-17 0.09074392918362771 18-19 0.03881559693352033 20-21 3.8007928453875477E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.14243471188089835 46-47 0.20790336864269887 48-49 0.0904113598096563 50-51 0.0643759288187516 52-53 0.07330779200541233 54-55 0.08162202635469759 56-57 0.11231342858120204 58-59 0.09345199408596634 60-61 0.14020174608423316 62-63 0.23811967176352988 64-65 0.1328377099462948 66-67 0.10523445190666772 68-69 0.10879769519921856 70-71 0.0898887507934155 72-73 0.07558826771264486 74-75 0.1393940776045883 76-77 0.21279688943113534 78-79 0.13806380010870267 80-81 0.08632550750086468 82-83 0.08922361204547269 84-85 0.09943824281745171 86-87 0.0722150640623634 88-89 0.09573246979319887 90-91 0.11611422142658959 92-93 0.16362413199393394 94-95 0.1318875117349479 96-97 0.08879602285036658 98-99 0.07900898127349365 100-101 0.06409086935534752 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1052412.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.93676892256855 #Duplication Level Percentage of deduplicated Percentage of total 1 73.25032307275238 41.70636718296794 2 14.308802025029472 16.29393908915768 3 5.170844020492603 8.83233453388298 4 2.492888104719785 5.67747975873001 5 1.3467806813575944 3.8340670221918387 6 0.8659100035882853 2.9581270669228026 7 0.5728450603344638 2.2831162780088747 8 0.3880373422635378 1.7674873991829356 9 0.2829639242681105 1.4499946412528937 >10 1.2597795020181175 11.699159782507774 >50 0.0448719797849377 1.7066874997336345 >100 0.015450464254685702 1.4311369128376328 >500 1.6793971196553712E-4 0.06588179810869005 >1k 3.3587942393107425E-4 0.29422103451442566 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1655 0.1572578039779098 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1422 0.13511818565352732 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 9.501982113468869E-5 7 0.0 0.0 0.0 0.0 9.501982113468869E-5 8 0.0 0.0 0.0 0.0 9.501982113468869E-5 9 0.0 0.0 0.0 0.0 9.501982113468869E-5 10-11 0.0 0.0 0.0 9.501982113468869E-5 9.501982113468869E-5 12-13 0.0 0.0 0.0 1.9003964226937739E-4 9.501982113468869E-5 14-15 0.0 0.0 0.0 4.7509910567344345E-4 9.501982113468869E-5 16-17 0.0 0.0 0.0 0.0019003964226937738 9.501982113468869E-5 18-19 0.0 0.0 0.0 0.0031831640080120715 9.501982113468869E-5 20-21 0.0 0.0 0.0 0.005226090162407878 9.501982113468869E-5 22-23 0.0 0.0 0.0 0.009169412739497458 1.4252973170203305E-4 24-25 0.0 0.0 0.0 0.012590126300346251 1.9003964226937739E-4 26-27 0.0 0.0 0.0 0.014823092097011437 1.9003964226937739E-4 28-29 0.0 0.0 0.0 0.016485938966868487 1.9003964226937739E-4 30-31 0.0 0.0 0.0 0.018766414674101017 1.9003964226937739E-4 32-33 0.0 0.0 0.0 0.02442009403161499 1.9003964226937739E-4 34-35 0.0 0.0 0.0 0.03168911034841868 2.850594634040661E-4 36-37 0.0 0.0 0.0 0.04252136995777319 2.850594634040661E-4 38-39 0.0 0.0 0.0 0.06128778463187421 2.850594634040661E-4 40-41 0.0 0.0 0.0 0.08337989304568932 2.850594634040661E-4 42-43 0.0 0.0 0.0 0.10727737806106354 2.850594634040661E-4 44-45 0.0 0.0 0.0 0.13853889921437612 2.850594634040661E-4 46-47 0.0 0.0 0.0 0.17749702587959848 2.850594634040661E-4 48-49 0.0 0.0 0.0 0.21849807869921667 2.850594634040661E-4 50-51 0.0 0.0 0.0 0.26016427026677763 2.850594634040661E-4 52-53 0.0 0.0 0.0 0.30567876459029353 2.850594634040661E-4 54-55 0.0 0.0 0.0 0.35879484460458455 2.850594634040661E-4 56-57 0.0 0.0 0.0 0.42511867975659723 2.850594634040661E-4 58-59 0.0 0.0 0.0 0.5029874231764746 3.3256937397141043E-4 60-61 0.0 0.0 0.0 0.5855121378319518 4.750991056734435E-4 62-63 0.0 0.0 0.0 0.6695571696255839 5.701189268081322E-4 64-65 0.0 0.0 0.0 0.7603486087197789 5.701189268081322E-4 66-67 0.0 0.0 0.0 0.8661056696426874 5.701189268081322E-4 68-69 0.0 0.0 0.0 0.9954751561175661 5.701189268081322E-4 70-71 0.0 0.0 0.0 1.1352493130066932 5.701189268081322E-4 72-73 0.0 0.0 0.0 1.2811522483590077 5.701189268081322E-4 74-75 0.0 0.0 0.0 1.4311410360201138 5.701189268081322E-4 76-77 0.0 4.7509910567344347E-5 0.0 1.5966655644367416 5.701189268081322E-4 78-79 0.0 9.501982113468869E-5 0.0 1.791978806779094 5.701189268081322E-4 80-81 0.0 9.501982113468869E-5 0.0 2.0161305648358248 5.701189268081322E-4 82-83 0.0 9.501982113468869E-5 0.0 2.254345256420489 6.651387479428209E-4 84-85 0.0 9.501982113468869E-5 0.0 2.492179868720615 6.651387479428209E-4 86-87 0.0 9.501982113468869E-5 0.0 2.7488759155159768 6.651387479428209E-4 88-89 0.0 9.501982113468869E-5 0.0 3.043579890765214 6.651387479428209E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1500 0.0 47.010323 1 GTATCAA 2525 0.0 37.927784 1 TCAACGC 2825 0.0 33.493965 4 CAACGCA 2900 0.0 33.119617 5 AACGCAG 2955 0.0 32.64386 6 ATCAACG 2900 0.0 32.627743 3 TATCAAC 3140 0.0 30.317112 2 ACGCAGA 3395 0.0 28.131866 7 CTGTCGA 85 6.392214E-4 27.952076 9 CGCAGAG 3420 0.0 27.788612 8 GTACATG 3110 0.0 26.503822 1 GTATAGG 450 0.0 24.352196 1 TACATGG 3255 0.0 23.835491 2 GCAGAGT 4000 0.0 23.640467 9 ACATGGG 3205 0.0 23.143473 3 ATAGCCC 340 0.0 22.375513 3 GAGTACT 2260 0.0 21.759731 12-13 GTGTAGC 595 0.0 21.620693 1 CATGGGG 1800 0.0 21.132431 4 AGAGTAC 3650 0.0 20.828552 10-11 >>END_MODULE