FastQCFastQC Report
Fri 10 Feb 2017
SRR2031605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052412
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19100.18148785836725542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17170.1631490328882605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.048.2268181
GTATCAA27900.038.4943161
CAACGCA31200.034.0987245
TCAACGC31250.034.04744
ATCAACG31600.033.519983
AACGCAG32200.033.039766
TATCAAC33750.031.3846362
GTGTACG804.4813563E-429.7010921
CGCAGAG36850.029.1283388
ACGCAGA36800.029.0388537
GTACATG32000.024.0578861
AATATCG1000.001654636323.747335
TACATGG32500.023.2380542
GCAGAGT45000.023.2196089
ACATGGG31950.022.4487463
CATGGGG17150.022.1570474
CTACACG1553.906544E-521.4512394
GAGTACT27300.021.1377312-13
GTATAGC5400.021.1207771
TATCGTA1353.7985368E-421.1087367
Produced by FastQC (version 0.11.5)