FastQCFastQC Report
Fri 10 Feb 2017
SRR2031604_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031604_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114167
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23750.034.297171
GTATCAA36250.033.771541
CAACGCA40050.029.5518515
TCAACGC40350.029.4499344
GTACATG65100.028.9761031
AACGCAG41550.028.4755486
ATCAACG42100.028.2230913
TACATGG64100.027.835162
TATCAAC44450.026.8672032
ACATGGG63500.026.4209023
ACGCAGA48450.024.2240587
CGCAGAG48350.023.8833228
CATGGGG37000.022.2245124
ATACCGT1503.0266752E-522.1743326
GTGTACG2551.1548764E-820.5484121
GAGTACA46550.019.2381781
TATAACG1759.915445E-519.0065697
GTGTAGC15500.018.746661
AGTACAT44100.018.6652
GTATTAG5650.018.5481241
Produced by FastQC (version 0.11.5)