##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031604_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2114167 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01238360072785 33.0 31.0 34.0 30.0 34.0 2 32.245027474177775 34.0 31.0 34.0 30.0 34.0 3 32.402441245180725 34.0 31.0 34.0 31.0 34.0 4 35.48099133133759 37.0 35.0 37.0 33.0 37.0 5 35.20070647209989 37.0 35.0 37.0 32.0 37.0 6 35.405206400440456 37.0 35.0 37.0 33.0 37.0 7 35.555171847824695 37.0 35.0 37.0 33.0 37.0 8 35.6332503534489 37.0 35.0 37.0 33.0 37.0 9 37.5772273429677 39.0 38.0 39.0 35.0 39.0 10-11 37.534435784874134 39.0 38.0 39.0 35.0 39.0 12-13 37.55875316377562 39.0 38.0 39.0 35.0 39.0 14-15 39.01290602871013 41.0 39.0 41.0 36.0 41.0 16-17 38.96928719443639 41.0 39.0 41.0 36.0 41.0 18-19 38.88682469265673 41.0 39.0 41.0 35.0 41.0 20-21 38.88412197333513 41.0 39.0 41.0 35.0 41.0 22-23 38.821672081722966 41.0 39.0 41.0 35.0 41.0 24-25 38.763876741998146 41.0 39.0 41.0 34.5 41.0 26-27 38.676535486553334 40.5 38.5 41.0 34.0 41.0 28-29 38.56254993101302 40.0 38.0 41.0 34.0 41.0 30-31 38.45301908505809 40.0 38.0 41.0 34.0 41.0 32-33 38.328138694814555 40.0 38.0 41.0 34.0 41.0 34-35 38.21264474376906 40.0 38.0 41.0 33.5 41.0 36-37 38.09740715846951 40.0 38.0 41.0 33.0 41.0 38-39 37.934432095477796 40.0 38.0 41.0 33.0 41.0 40-41 37.80396723626846 40.0 38.0 41.0 33.0 41.0 42-43 37.639474081281186 40.0 38.0 41.0 32.0 41.0 44-45 37.4443960671035 40.0 37.0 41.0 31.5 41.0 46-47 37.285531843037944 40.0 37.0 41.0 31.0 41.0 48-49 37.44357825091396 40.0 37.0 41.0 31.0 41.0 50-51 37.20863039674728 39.5 37.0 40.5 31.5 41.0 52-53 37.332057022931494 39.5 37.0 40.5 31.5 41.0 54-55 37.60279438663076 40.0 37.0 41.0 32.0 41.0 56-57 37.498341900143174 40.0 37.0 41.0 32.0 41.0 58-59 37.30276368896118 40.0 36.5 41.0 31.0 41.0 60-61 37.023062747644815 39.5 36.0 41.0 31.0 41.0 62-63 36.69424766350056 39.0 35.0 41.0 30.5 41.0 64-65 36.41559228764804 39.0 35.0 41.0 30.0 41.0 66-67 36.18950749869807 38.5 35.0 41.0 30.0 41.0 68-69 35.92206883372979 37.5 35.0 40.0 30.0 41.0 70-71 35.60906186691969 37.0 35.0 40.0 29.0 41.0 72-73 35.23895676169385 36.5 35.0 39.0 29.0 41.0 74-75 34.810062781227785 36.0 34.0 39.0 29.0 41.0 76-77 34.41150486219868 35.5 34.0 38.5 28.5 40.0 78-79 34.060892540655495 35.0 34.0 37.0 28.0 39.0 80-81 33.72896914009158 35.0 34.0 37.0 27.5 39.0 82-83 33.406029892624375 35.0 34.0 36.0 27.0 38.5 84-85 33.140759694007144 35.0 34.0 36.0 26.5 37.0 86-87 32.89297108506565 35.0 34.0 36.0 26.0 37.0 88-89 32.65313927423898 35.0 33.0 35.0 26.0 36.5 90-91 32.463308716861064 35.0 33.0 35.0 25.0 36.0 92-93 32.28984488926372 35.0 33.0 35.0 25.0 36.0 94-95 32.137805575434676 35.0 33.0 35.0 25.0 36.0 96-97 32.035689470131736 35.0 33.0 35.0 25.0 35.5 98-99 31.921114320675706 35.0 33.0 35.0 24.5 35.0 100-101 31.465800241891962 34.5 31.5 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09720138376342646 1101 2 0.21609394774339563 1101 3 0.26212095819300885 1101 4 0.21301165691840396 1101 5 0.31171444340167653 1101 6 0.22863788607055113 1101 7 0.2228186480157177 1101 8 0.18074408202195258 1101 9 0.29470766906297285 1101 10-11 0.44984427225025314 1101 12-13 0.3777790975267692 1101 14-15 0.42875004932796656 1101 16-17 0.41189797216271273 1101 18-19 0.47988237223187724 1101 20-21 0.23921196034256553 1101 22-23 0.3548875585026323 1101 24-25 0.4071224986780422 1101 26-27 0.3828403522953394 1101 28-29 0.39817600089970995 1101 30-31 0.32305712484939164 1101 32-33 0.5054890439194608 1101 34-35 0.3186150457369621 1101 36-37 0.33615487815976053 1101 38-39 0.21476519563574925 1101 40-41 0.21131814579430142 1101 42-43 0.17843326329180798 1101 44-45 0.26393342641326 1101 46-47 0.42333519882611625 1101 48-49 0.3834255962830895 1101 50-51 0.35224460942728086 1101 52-53 0.2508493045221343 1101 54-55 0.3552160721192337 1101 56-57 0.3680188321608995 1101 58-59 0.3915063330012245 1101 60-61 0.33936085726189447 1101 62-63 0.05328973151266325 1101 64-65 0.2358452740797503 1101 66-67 0.26029921842747683 1101 68-69 0.2839261763244352 1101 70-71 0.31501533278132854 1101 72-73 0.40637979362278287 1101 74-75 0.23779529834994406 1101 76-77 0.3013896941742118 1101 78-79 0.36793666544338777 1101 80-81 0.41070693345128007 1101 82-83 0.3902549628045122 1101 84-85 0.3004281007548073 1101 86-87 0.38087195844559574 1101 88-89 0.40767595734679674 1101 90-91 0.29842198891911664 1101 92-93 0.2791039465614986 1101 94-95 0.22627044873521385 1101 96-97 0.40777728880464537 1101 98-99 0.3720208645646501 1101 100-101 0.31913346688170563 1102 1 0.06767281120080071 1102 2 0.07734662695273187 1102 3 0.12179276213725387 1102 4 0.11719292456716346 1102 5 0.27188863453562107 1102 6 0.11477404093735544 1102 7 0.19277067741047205 1102 8 0.2439223897822984 1102 9 0.23312838147749204 1102 10-11 0.18724773565990205 1102 12-13 0.28640927024363094 1102 14-15 0.11080359949296792 1102 16-17 0.1926224076859171 1102 18-19 0.34653996644365037 1102 20-21 0.2548381298387099 1102 22-23 0.1867001215845434 1102 24-25 0.33920043106557785 1102 26-27 0.25380282635378393 1102 28-29 0.19876117424029616 1102 30-31 0.30579337724508804 1102 32-33 0.2692786083282215 1102 34-35 0.09095539888227222 1102 36-37 0.0632032683660455 1102 38-39 0.025303986050694505 1102 40-41 0.18401345873729014 1102 42-43 -0.05257752158894391 1102 44-45 -0.27514163681040316 1102 46-47 -0.7217299527405885 1102 48-49 -0.16379879954811827 1102 50-51 -0.12653605263511025 1102 52-53 -0.02104990050400346 1102 54-55 1.2018761158572033E-4 1102 56-57 0.06783851711989541 1102 58-59 0.09357938894424933 1102 60-61 -0.24067066951885607 1102 62-63 -0.6874376525583727 1102 64-65 -0.3430896197158191 1102 66-67 -0.10558154683409526 1102 68-69 0.059495189354748845 1102 70-71 0.0776447570206571 1102 72-73 -0.1326351645872208 1102 74-75 -0.17951267747196908 1102 76-77 -0.2230401587386126 1102 78-79 -0.1344187706581863 1102 80-81 -0.15996068582315104 1102 82-83 -0.29284645590062297 1102 84-85 -0.19899353024576527 1102 86-87 -0.19554079288975856 1102 88-89 -0.13069485656581747 1102 90-91 -0.16038679775344633 1102 92-93 -0.2193832215851117 1102 94-95 -0.13461933227841172 1102 96-97 -0.09521095102570598 1102 98-99 -0.3748905315727562 1102 100-101 -0.10703149302938542 1103 1 -0.016373391549969085 1103 2 -0.04375619619899851 1103 3 0.006789457444590141 1103 4 0.12227270929003708 1103 5 0.053168337428019186 1103 6 0.12317110762920436 1103 7 0.09328998625760221 1103 8 0.10125408650593215 1103 9 0.37721298160968075 1103 10-11 0.26520700925699003 1103 12-13 0.26141320440156335 1103 14-15 0.3418928891414126 1103 16-17 0.2894971126240691 1103 18-19 0.2725305874111399 1103 20-21 0.029464384835087287 1103 22-23 0.15690123573806858 1103 24-25 0.3504867433432963 1103 26-27 0.2767248041336572 1103 28-29 0.15532492333544212 1103 30-31 0.2204410340606202 1103 32-33 0.21838948863539542 1103 34-35 0.30875352937042067 1103 36-37 0.3783985599658308 1103 38-39 0.2566482091331963 1103 40-41 0.1821061789714591 1103 42-43 0.14572292218406346 1103 44-45 0.23350406866279627 1103 46-47 0.3569343219580574 1103 48-49 0.2160960797519138 1103 50-51 0.40839297515578465 1103 52-53 0.25547733960551966 1103 54-55 0.275165037012016 1103 56-57 0.17243046625910097 1103 58-59 0.28088622178974276 1103 60-61 0.2958131374870945 1103 62-63 0.47758296242918874 1103 64-65 0.501095694859444 1103 66-67 0.2944121585132109 1103 68-69 0.16098702204287463 1103 70-71 0.2641773475849689 1103 72-73 0.279651997468612 1103 74-75 0.2571977370309142 1103 76-77 -0.10953498138677276 1103 78-79 0.10054173039617353 1103 80-81 0.16921188208041116 1103 82-83 0.07707139888173486 1103 84-85 -0.04650556876904233 1103 86-87 0.02293086543649281 1103 88-89 0.24663809908835788 1103 90-91 0.17954333160166414 1103 92-93 -0.07228663013954417 1103 94-95 -0.055281529741662894 1103 96-97 0.047378669092005055 1103 98-99 0.024860829645888316 1103 100-101 -0.0031036297492406106 1104 1 0.10499442071834153 1104 2 0.19651229002136716 1104 3 0.1827398900560837 1104 4 0.1168723701333505 1104 5 0.10685503452129552 1104 6 0.04300208228708868 1104 7 0.039223312806662136 1104 8 0.13574869942176804 1104 9 0.2253338944765133 1104 10-11 0.23012860670838364 1104 12-13 0.2504855347398447 1104 14-15 0.2940071739435268 1104 16-17 0.2708082904567135 1104 18-19 0.26466054445642584 1104 20-21 0.2306698477149851 1104 22-23 0.30138503657344273 1104 24-25 0.15164594203501025 1104 26-27 0.3856456114166704 1104 28-29 0.2231492779101103 1104 30-31 0.21522227400357252 1104 32-33 0.18706609625893122 1104 34-35 0.24559649879586232 1104 36-37 0.2853865902829824 1104 38-39 0.22608776253412088 1104 40-41 0.16227186918725067 1104 42-43 2.3829839914668582E-4 1104 44-45 0.13082921183806206 1104 46-47 0.2083520214449308 1104 48-49 0.3078895881138166 1104 50-51 0.30056378037214415 1104 52-53 0.18556340011725325 1104 54-55 0.12885532577979575 1104 56-57 0.15147233292645268 1104 58-59 0.1667904101668043 1104 60-61 0.2885509711885703 1104 62-63 0.3767032302198814 1104 64-65 0.3771324701856713 1104 66-67 0.14348686049687132 1104 68-69 0.16382401731640073 1104 70-71 0.18587157465234583 1104 72-73 0.060910468903252024 1104 74-75 0.056879503268199016 1104 76-77 0.13910680979839896 1104 78-79 0.15399097789760674 1104 80-81 0.30924400191235435 1104 82-83 0.16404165896172884 1104 84-85 0.06259410960506528 1104 86-87 0.10190551648108936 1104 88-89 0.11580821989119983 1104 90-91 0.21681279812113274 1104 92-93 0.22947745214629123 1104 94-95 0.20657847875863666 1104 96-97 0.17739985141994197 1104 98-99 0.27238378571942334 1104 100-101 0.3354183904935475 1105 1 0.13586672413373435 1105 2 0.18780545837262252 1105 3 0.12079768492014153 1105 4 0.1547095274524466 1105 5 0.10148319536465067 1105 6 0.06429600934913537 1105 7 0.21906000378264423 1105 8 0.1917487816849146 1105 9 0.2846400659634938 1105 10-11 0.2586587726881717 1105 12-13 0.24364199118803498 1105 14-15 0.1695981971442322 1105 16-17 0.21100284280205983 1105 18-19 0.2565512322958483 1105 20-21 0.2555864103715706 1105 22-23 0.20628911524146787 1105 24-25 0.2603223696688701 1105 26-27 0.17795909292927092 1105 28-29 0.18393101682521973 1105 30-31 0.15326645098162572 1105 32-33 0.20599301239970913 1105 34-35 0.20871467786773223 1105 36-37 0.19973356123436758 1105 38-39 0.19787317021889095 1105 40-41 0.27137378627989506 1105 42-43 0.21252113133074602 1105 44-45 0.25420086701429057 1105 46-47 0.4157064302633202 1105 48-49 0.37169258010233364 1105 50-51 0.3166494218676732 1105 52-53 0.2325922136186378 1105 54-55 0.28103881484338444 1105 56-57 0.3479492468578016 1105 58-59 0.37298479743002133 1105 60-61 0.406253139128232 1105 62-63 0.3111210197368379 1105 64-65 0.2616205585651983 1105 66-67 0.23926312029723107 1105 68-69 0.3863131778960138 1105 70-71 0.4489036831263107 1105 72-73 0.304186637686108 1105 74-75 0.29424314723663514 1105 76-77 0.3230178888935882 1105 78-79 0.2679624009027748 1105 80-81 0.3614610910253049 1105 82-83 0.18294467432297523 1105 84-85 0.22328085681735388 1105 86-87 0.2735372921783821 1105 88-89 0.33093330017418765 1105 90-91 0.2954685960472645 1105 92-93 0.280852177738943 1105 94-95 0.2793910919225695 1105 96-97 0.17299085238736467 1105 98-99 0.17645369939036826 1105 100-101 0.2016094594392399 1106 1 0.24880994690614244 1106 2 0.14749979460277274 1106 3 0.17137855390077306 1106 4 0.24921509513751516 1106 5 0.27288988610008147 1106 6 0.09238891667488502 1106 7 0.15663026379822043 1106 8 0.16555061554068828 1106 9 0.1517766918654786 1106 10-11 0.1460057069335221 1106 12-13 0.16388408995874926 1106 14-15 0.17705903783851795 1106 16-17 0.3060379866834708 1106 18-19 0.2756924785123118 1106 20-21 0.15871201982022853 1106 22-23 0.2453061171742661 1106 24-25 0.2220331386166805 1106 26-27 0.1117145016622132 1106 28-29 0.24104319343096137 1106 30-31 0.2905689896176966 1106 32-33 0.21235030509203057 1106 34-35 0.21368619332770322 1106 36-37 0.340102964414676 1106 38-39 0.26149207830888344 1106 40-41 0.24340019215406272 1106 42-43 0.05996196228971229 1106 44-45 0.2289021083472278 1106 46-47 0.4933424592029141 1106 48-49 0.310138860622331 1106 50-51 0.05235720679632294 1106 52-53 0.0467014553574856 1106 54-55 0.2991993937621231 1106 56-57 0.29343897006702946 1106 58-59 0.28711893242121533 1106 60-61 0.37200589046683064 1106 62-63 0.36395694783497134 1106 64-65 0.4282555140823803 1106 66-67 0.2342480020067157 1106 68-69 0.37455270172139166 1106 70-71 0.3271590308152881 1106 72-73 0.4189203512361246 1106 74-75 0.4392697291076857 1106 76-77 0.5020447688494514 1106 78-79 0.40880192187415076 1106 80-81 0.29457474537059625 1106 82-83 0.3420811321558688 1106 84-85 0.34811893176031816 1106 86-87 0.18938977508211252 1106 88-89 0.20898569650648113 1106 90-91 0.22144763814856816 1106 92-93 0.3084551478051978 1106 94-95 0.34569236686933635 1106 96-97 0.4285985727838195 1106 98-99 0.21727229100308065 1106 100-101 0.34581432614934826 1107 1 0.10740948408006545 1107 2 0.04275878403346667 1107 3 0.09460717973308874 1107 4 0.2341091852348569 1107 5 0.17275594644092962 1107 6 0.08637345891466452 1107 7 0.17346093016526964 1107 8 0.3156615912994809 1107 9 0.307270878943406 1107 10-11 0.2883414805508622 1107 12-13 0.2614678539884139 1107 14-15 0.1679910075301123 1107 16-17 0.23050769798880566 1107 18-19 0.20009763464712194 1107 20-21 0.016965373732368505 1107 22-23 0.07513868767245668 1107 24-25 0.1296135418351767 1107 26-27 0.12725948528194664 1107 28-29 0.2125829870121052 1107 30-31 0.15333381365311283 1107 32-33 0.17045178187780152 1107 34-35 0.23421227361659192 1107 36-37 0.22448268285557305 1107 38-39 0.26952599391999144 1107 40-41 0.29647735480156 1107 42-43 0.32972964252183345 1107 44-45 0.22731656436889836 1107 46-47 0.500189495972748 1107 48-49 0.37960712313223866 1107 50-51 0.08586503620509234 1107 52-53 0.25707492919440966 1107 54-55 0.3105758874012281 1107 56-57 0.2480759039034055 1107 58-59 0.3236564197565741 1107 60-61 0.33001102728437814 1107 62-63 0.3103781274501287 1107 64-65 0.33961904740861826 1107 66-67 0.21989679625411185 1107 68-69 0.2609555594950166 1107 70-71 0.2542826495317527 1107 72-73 0.26408428194624634 1107 74-75 0.40563525329567085 1107 76-77 0.41822037271132473 1107 78-79 0.4484644215291951 1107 80-81 0.358391966521836 1107 82-83 0.33825660789297984 1107 84-85 0.43232942695267695 1107 86-87 0.3544011683305328 1107 88-89 0.336426719691147 1107 90-91 0.28867790594904363 1107 92-93 0.359704561388007 1107 94-95 0.4171832759244083 1107 96-97 0.48404604360093373 1107 98-99 0.3124830365315283 1107 100-101 0.31318957205034437 1108 1 0.11192138748804581 1108 2 -0.009706076256009055 1108 3 0.10552290968261957 1108 4 0.037477358417369544 1108 5 0.14048534969509063 1108 6 0.12604348402844323 1108 7 0.09021584618233902 1108 8 0.13363984839967458 1108 9 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2316 70-71 -1.2412345449788447 2316 72-73 -1.1000856453369394 2316 74-75 -1.2043314790171067 2316 76-77 -0.9607553563003606 2316 78-79 -1.1260225916737667 2316 80-81 -1.2046399622159853 2316 82-83 -1.2824189181073606 2316 84-85 -1.5880682236682837 2316 86-87 -1.1356758212600795 2316 88-89 -1.0034843290995283 2316 90-91 -1.1942100929578174 2316 92-93 -1.506372844636111 2316 94-95 -1.485268711316536 2316 96-97 -1.3153627798731833 2316 98-99 -0.9950813949861264 2316 100-101 -1.4342354626885943 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 79.0 8 382.0 9 485.0 10 599.0 11 768.0 12 1132.0 13 1571.0 14 2095.0 15 2580.0 16 3357.0 17 4434.0 18 5393.0 19 6734.0 20 8266.0 21 9827.0 22 11010.0 23 12096.0 24 13214.0 25 14332.0 26 16445.0 27 18785.0 28 22182.0 29 25933.0 30 31608.0 31 38820.0 32 48515.0 33 63176.0 34 88677.0 35 134226.0 36 232626.0 37 446835.0 38 620029.0 39 227690.0 40 262.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.32482259056968 18.944404980530695 12.647659050791246 24.08311337810838 2 15.034874917826196 19.68321850755762 41.59354285971953 23.688363714896656 3 18.890956065494223 24.58890775990507 29.64801974380068 26.872116430800023 4 11.964694979889694 16.06349973810387 38.87884756223292 33.092957719773516 5 12.98188136955321 38.29140105871783 35.75654705320377 12.970170518525192 6 32.04015308327457 37.343571109038656 17.845641031473434 12.77063477621333 7 28.35449947805287 32.056225291619896 21.872119872988915 17.71715535733832 8 25.48707045041963 37.19846411991351 19.83522885854806 17.4792365711188 9 26.086713416607555 15.818168981182035 20.04727117477114 38.04784642743927 10-11 24.73084953093711 26.485472450152514 28.956266055135 19.827411963775372 12-13 25.009163195866833 23.633961185010318 29.141687530163914 22.215188088958936 14-15 22.196691960502946 25.514446489603493 26.15018870857368 26.13867284131988 16-17 22.36004571095302 28.78914260719067 26.70717817235718 22.143633509499132 18-19 22.175136698381575 28.063955348034362 28.11150878295382 21.64939917063024 20-21 23.036481435276226 27.371021317002164 27.927578716344453 21.664918531377158 22-23 22.524923527800784 27.30628658946999 27.68203741710092 22.486752465628307 24-25 22.39813600344722 27.373310623049175 27.79224630788391 22.436307065619697 26-27 22.542755610129188 27.495084352371407 27.650488348366046 22.311671689133355 28-29 22.52343357927732 27.42456012226092 27.852908497767682 22.19909780069408 30-31 22.649795404052757 27.49059085682446 27.662857286108427 22.196756453014356 32-33 22.481076471253218 27.592309406021382 27.572301525849184 22.354312596876216 34-35 22.594099709247185 27.58582931244315 27.70407919525752 22.115991783052145 36-37 22.684702769459555 27.41254593416698 27.54742175050505 22.35532954586842 38-39 22.472255029995264 27.589258559044765 27.701264848046534 22.23722156291343 40-41 22.620185633396037 27.488888058511936 27.66004293889745 22.230883369194583 42-43 22.486161216214235 27.495391802066727 27.623834824779685 22.394612156939353 44-45 22.580357912546265 27.602543905435756 27.46662864797119 22.350469534046784 46-47 22.590257586310596 27.46942713333474 27.415228620795972 22.525086659558692 48-49 22.381177359469525 27.46224503176762 27.601377788276775 22.55519982048608 50-51 22.482368426408385 27.59884969394116 27.32348429522692 22.595297584423538 52-53 22.488844079818524 27.583682713914992 27.44267614283838 22.484797063428108 54-55 22.50959348431232 27.412406571072257 27.498276332584854 22.579723612030573 56-57 22.487608598632242 27.64276303094802 27.315821810050206 22.553806560369534 58-59 22.54460365067003 27.592858681526266 27.420953832824786 22.44158383497892 60-61 22.68646825917486 27.58411540102985 27.368690545605602 22.360725794189687 62-63 22.43602782444107 27.52848278468205 27.503969738478514 22.531519652398366 64-65 22.480718001497138 27.480716817426487 27.493504780448436 22.545060400627936 66-67 22.552439150556904 27.495442106765854 27.369755285936925 22.58236345674032 68-69 22.494916962052645 27.523383868563577 27.295014124547922 22.68668504483585 70-71 22.442214616579253 27.2680211170606 27.45733897862564 22.83242528773451 72-73 22.459890643441906 27.359280290614002 27.474918117484343 22.705910948459753 74-75 22.459852268663575 27.400018472337674 27.374891356603744 22.76523790239501 76-77 22.312939583801416 27.394217852494187 27.48501421552037 22.807828348184024 78-79 22.393505928513964 27.403690510896357 27.325349391210402 22.87745416937928 80-81 22.538029059825824 27.40007252647785 27.233929136139583 22.827969277556747 82-83 22.535288947676516 27.393781646314952 27.352570868453824 22.71835853755471 84-85 22.700693450018594 27.242905885341294 27.337123994582697 22.719276670057415 86-87 22.440421319694835 27.379248076169834 27.34781831801093 22.832512286124402 88-89 22.519009701208827 27.252556681127025 27.342509815544 22.885923802120146 90-91 22.599483375438314 27.246547352121546 27.391353759970833 22.76261551246931 92-93 22.60818791024235 27.166565204105563 27.42035583749251 22.80489104815958 94-95 22.47964191364591 27.253242564574233 27.42390759697383 22.843207924806034 96-97 22.541167837423583 27.114540860106878 27.52128962770522 22.82300167476432 98-99 22.52422795401156 27.139190172722916 27.302078710524913 23.03450316274061 100-101 22.5419781174701 27.037708245871528 27.365207847116686 23.05510578954169 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 46.0 1 32.5 2 36.5 3 66.0 4 62.5 5 58.5 6 106.0 7 197.5 8 304.5 9 386.0 10 388.0 11 376.5 12 400.5 13 427.0 14 490.0 15 558.5 16 613.0 17 685.5 18 765.5 19 897.0 20 1048.0 21 1239.5 22 1519.5 23 1833.0 24 2314.5 25 2999.0 26 4198.0 27 6261.5 28 9301.5 29 14414.5 30 21985.0 31 31199.5 32 40882.5 33 49881.5 34 58773.0 35 65783.0 36 70262.5 37 72199.0 38 70424.5 39 67733.5 40 66218.0 41 65735.5 42 66460.5 43 68270.0 44 70611.0 45 73484.0 46 76243.5 47 78669.5 48 81300.5 49 82243.0 50 80910.0 51 78799.5 52 76762.0 53 75590.5 54 73096.0 55 67400.0 56 59990.0 57 52542.0 58 46707.0 59 40631.5 60 32927.0 61 25726.5 62 19802.0 63 14735.0 64 10544.5 65 7546.0 66 5352.0 67 3829.5 68 2826.0 69 2090.5 70 1559.0 71 1162.0 72 829.5 73 624.5 74 485.5 75 368.0 76 261.0 77 174.5 78 111.5 79 75.5 80 60.5 81 51.5 82 39.5 83 34.5 84 30.5 85 22.5 86 19.5 87 14.0 88 12.0 89 17.0 90 12.5 91 8.5 92 6.0 93 2.5 94 2.0 95 0.5 96 1.5 97 4.5 98 3.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.452755151319645 2 0.3477492553804879 3 0.1861253155498123 4 0.21530938662839785 5 0.2370673650662412 6 0.18650371517481826 7 0.17973982187783652 8 0.18191561972162085 9 0.17926682234657904 10-11 0.19182495990146475 12-13 0.18080407082316582 14-15 0.19085531086238694 16-17 0.083389817360691 18-19 0.03571146460993857 20-21 4.493495546945913E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.13835236289280836 46-47 0.204832447011045 48-49 0.08495071581384063 50-51 0.06328733728224875 52-53 0.0709262797120568 54-55 0.07877807193093071 56-57 0.1097122412751689 58-59 0.09232950850145707 60-61 0.1414268598459819 62-63 0.24056756159754644 64-65 0.13274731844740742 66-67 0.10271184821255842 68-69 0.10599919495479779 70-71 0.08733936344669083 72-73 0.07267637797770941 74-75 0.1372644639709162 76-77 0.21289708901898477 78-79 0.13657861465059287 80-81 0.08679541398574475 82-83 0.08776506302482255 84-85 0.09679935407184012 86-87 0.07187227877457174 88-89 0.09251870831396006 90-91 0.11266848834552805 92-93 0.15949544194001705 94-95 0.1287031724551561 96-97 0.09126525955612778 98-99 0.07944027127469117 100-101 0.06338193718850024 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2114167.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.54468731830673 #Duplication Level Percentage of deduplicated Percentage of total 1 64.78376639849563 30.153401503202048 2 15.993006790836848 14.887790007181145 3 6.932137954812616 9.679625806623585 4 3.8262659004689143 7.123693997360997 5 2.3279086290815565 5.417588962309456 6 1.4999177887176878 4.1887922687418495 7 1.0349241726280245 3.3719155409190087 8 0.7052235991820293 2.6259529530734738 9 0.5425275231212721 2.272659653272953 >10 2.282001021064873 17.046802096639876 >50 0.054113545415826525 1.6958039961659355 >100 0.017695686435562838 1.3091703481483723 >500 3.0659384347150436E-4 0.09370839906285026 >1k 2.043958956476675E-4 0.1330944672984024 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.7299953125746455E-5 0.0 6 0.0 0.0 0.0 4.7299953125746455E-5 0.0 7 0.0 0.0 0.0 4.7299953125746455E-5 0.0 8 0.0 0.0 0.0 4.7299953125746455E-5 0.0 9 0.0 0.0 0.0 4.7299953125746455E-5 0.0 10-11 0.0 0.0 0.0 4.7299953125746455E-5 0.0 12-13 0.0 0.0 0.0 7.094992968861968E-5 0.0 14-15 0.0 0.0 0.0 3.547496484430984E-4 0.0 16-17 0.0 0.0 0.0 8.986991093891826E-4 0.0 18-19 0.0 0.0 0.0 0.001253448757832281 0.0 20-21 0.0 0.0 0.0 0.001962948054718478 0.0 22-23 0.0 0.0 0.0 0.0038785961563112094 0.0 24-25 0.0 0.0 0.0 0.0058415442110296865 0.0 26-27 0.0 0.0 0.0 0.007142292921987715 0.0 28-29 0.0 0.0 0.0 0.008939691140766079 0.0 30-31 0.0 0.0 0.0 0.013362236758023373 0.0 32-33 0.0 0.0 0.0 0.02322427698474151 0.0 34-35 0.0 0.0 0.0 0.0389515113990522 0.0 36-37 0.0 0.0 0.0 0.06255418800879968 0.0 38-39 0.0 0.0 0.0 0.1022861486344267 0.0 40-41 0.0 0.0 0.0 0.14906580227578994 0.0 42-43 0.0 0.0 0.0 0.19478120697182388 0.0 44-45 0.0 0.0 0.0 0.24879775344142632 0.0 46-47 0.0 0.0 0.0 0.31471023812215404 0.0 48-49 0.0 0.0 0.0 0.3817579216778996 0.0 50-51 0.0 0.0 0.0 0.4576270464915969 0.0 52-53 0.0 0.0 0.0 0.5299250248443004 0.0 54-55 0.0 0.0 0.0 0.6135276919940573 0.0 56-57 0.0 0.0 0.0 0.7119588944487356 0.0 58-59 0.0 0.0 0.0 0.8192824880910543 0.0 60-61 0.0 0.0 0.0 0.9384547199913724 0.0 62-63 0.0 0.0 0.0 1.058289151235451 0.0 64-65 0.0 0.0 0.0 1.1822386784014696 0.0 66-67 0.0 0.0 0.0 1.3228850890208768 2.3649976562873227E-5 68-69 0.0 0.0 0.0 1.4877017756875404 4.7299953125746455E-5 70-71 0.0 0.0 0.0 1.6698775451513528 1.1824988281436614E-4 72-73 0.0 0.0 0.0 1.8527155139589255 1.4189985937723936E-4 74-75 0.0 0.0 0.0 2.0399287284306302 1.4189985937723936E-4 76-77 0.0 0.0 0.0 2.2434131267775914 1.4189985937723936E-4 78-79 0.0 0.0 0.0 2.486203786172048 1.4189985937723936E-4 80-81 0.0 0.0 0.0 2.761371263481078 1.4189985937723936E-4 82-83 0.0 0.0 0.0 3.0483400790949817 1.4189985937723936E-4 84-85 0.0 0.0 0.0 3.3335351464666703 1.4189985937723936E-4 86-87 0.0 0.0 0.0 3.633464149237028 1.4189985937723936E-4 88-89 0.0 0.0 0.0 3.9761523096330613 1.4189985937723936E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2375 0.0 34.29717 1 GTATCAA 3625 0.0 33.77154 1 CAACGCA 4005 0.0 29.551851 5 TCAACGC 4035 0.0 29.449934 4 GTACATG 6510 0.0 28.976103 1 AACGCAG 4155 0.0 28.475548 6 ATCAACG 4210 0.0 28.223091 3 TACATGG 6410 0.0 27.83516 2 TATCAAC 4445 0.0 26.867203 2 ACATGGG 6350 0.0 26.420902 3 ACGCAGA 4845 0.0 24.224058 7 CGCAGAG 4835 0.0 23.883322 8 CATGGGG 3700 0.0 22.224512 4 ATACCGT 150 3.0266752E-5 22.174332 6 GTGTACG 255 1.1548764E-8 20.548412 1 GAGTACA 4655 0.0 19.238178 1 TATAACG 175 9.915445E-5 19.006569 7 GTGTAGC 1550 0.0 18.74666 1 AGTACAT 4410 0.0 18.665 2 GTATTAG 565 0.0 18.548124 1 >>END_MODULE