FastQCFastQC Report
Fri 10 Feb 2017
SRR2031604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114167
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.039.4793931
GTATCAA35750.032.695051
GTACATG67050.028.7706851
TACATGG66000.028.1405452
TCAACGC40500.028.0312564
ATCAACG41350.027.4550383
CAACGCA41450.027.3855635
ACATGGG65550.026.5221083
AACGCAG43300.026.32526
TATCAAC46550.024.8983062
GTATAGG6550.023.2130181
ACGCAGA52000.022.0121277
CATGGGG37800.021.7397214
CGCAGAG52400.021.3908988
GTATATA12150.018.379961
GAGTACA47300.017.6796841
CCTATAC6850.017.3360083
GCAGAGT62450.017.1119069
GTATTAT10950.016.9228481
GCGTACA3652.2846507E-916.9228481
Produced by FastQC (version 0.11.5)