FastQCFastQC Report
Fri 10 Feb 2017
SRR2031603_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031603_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884525
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31720.16831827648877037No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG52050.027.9145621
TACATGG53450.025.2827852
ACATGGG51850.024.5697773
CATGGGG32500.022.3792934
CGTATAC1200.00474424319.8063263
GTATAGC7900.019.2963241
TACACCG2251.6561571E-619.0150855
ATGGGGG15700.018.4700685
TAGGACG1300.00753032818.2837354
GTGTACG3156.5738277E-918.1477341
GAGTACA37550.018.0148281
TATACTG10150.017.797375
TGTACGC2152.3599418E-517.7054022
TAGCCCT13500.017.606564
GTGTAGC13400.017.4197921
GTATAGA9650.017.277961
GTCCTAT5250.017.2403471
AGTACAT37650.016.9353472
AGAGTAC42700.016.63801410-11
ACCGTAT2002.7515262E-416.632898
Produced by FastQC (version 0.11.5)