FastQCFastQC Report
Fri 10 Feb 2017
SRR2031603_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031603_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884525
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34250.18174341014313952No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1004.9574002E-528.497785
GTACATG49600.026.4469341
TACATGG49350.025.893012
ACATGGG49700.024.8504543
CATGGGG29550.021.7016944
GTATAGG6700.021.2811161
TACACCG1856.5601853E-620.5389395
GTATAGA10700.020.4325241
TACACTG16750.020.132765
TAATACT13750.019.6919884
GTCTAGA9950.019.5843281
ATACGCT1507.7207177E-419.0010414
TATACTG9800.018.9015885
CCTACAC9550.017.906743
ATTCGCG800.001191685617.81252980-81
CTACACT9700.017.6298314
CGTTACT1350.00939960417.5935552
TTATACT11750.017.383934
GAGTACA38600.017.2380711
TTAATAC12750.017.1381933
Produced by FastQC (version 0.11.5)