FastQCFastQC Report
Fri 10 Feb 2017
SRR2031602_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031602_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054553
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14310.13569730492445614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11480.1088612900442178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21000.028.1309431
GTATCAA29100.026.030711
GTCTACG1109.286678E-525.9860611
GTACATG29300.024.0644531
TACATGG28550.023.8395542
TCAACGC31100.023.6886544
ATCAACG31300.023.5372893
CAACGCA31550.023.200135
ACATGGG27400.023.07123
TATCAAC33100.022.4318352
AACGCAG33050.022.4230776
GTGTAGC7600.021.9399851
GTATAGG3700.021.889161
GTATAAG3850.021.0363331
CATGGGG17100.020.8465524
GGACCGA1150.003720668420.6544766
GTACCTG8450.020.2968041
GTAATAC3102.564775E-1019.9319933
ACGCAGA36850.019.850137
TAGCCCT9100.019.8477524
Produced by FastQC (version 0.11.5)